Browsing by Subject "Biochemistry"
Now showing items 21-38 of 38
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Novel diagnostic and analytical applications of benchtop time-domain NMR.
(East Carolina University, 2015)High-resolution nuclear magnetic resonance (NMR) spectroscopy is a powerful technique, yet practical application outside of the research laboratory has been limited due to the high cost and complexity of the instrumentation. ... -
Paired Effects of Dietary Leucine Supplementation and Overload on Protein Translational Signaling and Hypertrophy in Aged Rat Skeletal Muscle
(East Carolina University, 2012)Sarcopenia is an age-associated disorder that causes loss of skeletal muscle mass, particularly in type II (fast-twitch) muscle fibers. This loss in muscle mass can cause disability, reductions in the quality of life, and ... -
Post-Transcriptional Regulation of mRNA Metabolism during Differentiation of 3T3-L1 Cells: Role of HuR
(East Carolina University, 2010)Our studies address early control points in the differentiation process that are necessary for expression as well as the maintenance of the adipocyte phenotype. We have focused on the post-transcriptional regulation of ... -
Proposed regulatory role of noncatalytic Adams in ectodomain shedding
(East Carolina University, 2015)Members of the ADAM (A Disintegrin And Metalloprotease) protein family uniquely exhibit both proteolytic and adhesive properties. Specifically, ADAMs catalyze the conversion of cell-surface proteins to soluble, biologically ... -
Prothymosin-Alpha : Features of the protein sequence that contribute to the anti-HIV Activity
(East Carolina University, 2010)Prothymosin-alpha (ProT[alpha]) is a protein mainly located in the nucleus of the cells. Though its exact function is not known, it is believed to be involved in cell proliferation. The ability of this protein to inhibit ... -
Resolving the Role of POU1F1 in Human Growth Hormone Locus Activation
(East Carolina University, 2012)The human growth hormone gene ( hGH-N) is regulated by a distal locus control region (LCR) composed of five deoxyribonuclease I hypersensitive sites (HSs). The region encompassing HSI and HSII contains the predominant ... -
RNA polymerase II CTD Evolutionary Diversity and Associated Protein Identification in Green and Red Algae
(East Carolina University, 2014)In model eukaryotes, the C-terminal domain (CTD) of the largest subunit (RPB1) of DNA-dependent RNA polymerase II is composed of tandemly repeated heptads with the consensus sequence YSPTSPS. Both the core motif and tandem ... -
The Role of eIF4G-1 Isoforms and Live Cell Imaging of IRES-mediated Translation Initiation in Breast Cancer Cells
(East Carolina University, 2012)The development of cancer is a consequence of mutations that lead to dysfunctional cell processes such as unrestrained cell proliferation, resistance to apoptosis, and improper regulation of cell processes such as translation. ... -
Role of Notch signaling in tumorigenesis, stemness, and epithelial to mesenchymal transtion in colorectal cancer.
(East Carolina University, 2015)Colorectal cancer is the third most commonly diagnosed cancer in both men and women in the United States. Surgical resection and combination chemotherapy are often used for treatment, but in later stages of the disease, ... -
Selective Recruitment of Germ Cell mRNAs by eIF4 Factors
(East Carolina University, 1/13/16)During germ line development critical steps in the proliferation and differentiation of stem cells into oocytes and sperm are regulated by mRNA translational control. This regulation changes the subset of proteins being ... -
Synthesis Of Non-Natural Fmoc-Protected Amino Acids To Provide Novel Fluorescent Anion Probes And Their Incorporation Into Synthetic Peptides
(East Carolina University, 2015)Cystic fibrosis is a genetic disorder caused by mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. CFTR serves to control the gradient of chloride and bicarbonate ions across the cellular ... -
The therapeutic potential of Rho GTPase intervention
(East Carolina University, 2013)Small GTPases of the Rho family are well established regulators of critical cellular functions including cytoskeletal remodeling, motility, vesicle trafficking and cell cycle control. Additionally, aberrant signaling ... -
Thermodynamic and Spectroscopic Investigations of Novel Antimicrobial Peptides containing Unnatural Amino Acids with Model Membrane Systems
(East Carolina University, 2011)With the over prescription and use of antibiotic drugs the evolution of drug resistant bacteria strains has become a world wide health crisis. In response to this crisis, the search for new drugs to kill bacteria via a ... -
Thermodynamic and spectroscopic studies of Cd²⁺ binding to the regulatory domain and full length human cardiac troponin C (HcTnC) : elucidating plausible Cd²⁺ binding sites
(East Carolina University, 2015)Toxic metals such as cadmium (Cd²⁺) have been shown to bind to and interfere with various calcium (Ca²⁺) binding proteins including the regulatory protein cardiac troponin C (cTnC). Recent structural data has shown that ... -
Thermodynamic Investigation of Copper Binding to the Amyloid-Beta (A[beta]) Peptide
(East Carolina University, 2010)Alzheimer's disease is a fatal and neurodegenerative disease with as many as 5.3 million Americans living with the disease. One of the characteristic hallmarks of Alzheimer's is the amyloid plaques that build up around the ... -
Thermodynamic studies of Ca²⁺ binding to human cardiac troponin C
(East Carolina University, 2012)Human Cardiac Troponin C (HcTnC) is an EF hand protein responsible for initiating contraction of the myocardium (heart muscle). The helix-loop-helix motif, characteristic of members of the EF-hand family, allows HcTnC to ... -
The unique roles of IFE-1, a germline-specific isoform of eukaryotic translation factor 4E, during gametogenesis
(East Carolina University, 2009)Fertility and embryonic viability are measures of efficient germ cell growth and differentiation. During oogenesis, spermatogenesis and embryogenesis cells initially proliferate then differentiate into specific tissues. ... -
Using TIRF microscopy to analyze stimulated and basal state B-cell MHC II clustering in response to ageing and dietary fish oil
(East Carolina University, 2013)This research focused on developing an efficient TIRF microscopy approach to evaluate membrane protein organization. More specifically, the data demonstrate that TIRF microscopy can detect changes in ex vivo B-cell MHC II ...