Browsing by Subject "Caldesmon"

Now showing items 1-4 of 4

  • Characterization of a caldesmon fragment that competes with myosin-ATP binding to actin. 

    Velaz, Laly; Chen, Yi-Der; Chalovich, Joseph (East Carolina University, 1993-08)
    The protein caldesmon inhibits actin-activated ATP hydrolysis of myosin and inhibits the binding of myosin*ATP to actin. Afragment isolated from a chymotryptic digest of caldesmon contains features of the intact molecule ...

  • The Interaction of Caldesmon with the COOH Terminus of Actin 

    Crosbie, Rachelle; Adams, Susan; Chalovich, Joseph; Reisler, Emil (East Carolina University, 1991-10-25)
    Caldesmon interacts with the NH2-terminal region of actin. It is now shown in airfuge centrifugation experiments that modification of the penultimate cysteine residue of actin significantly weakens its binding to caldesmon ...

  • Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity 

    Chalovich, Joseph; Bryan, Joseph; Benson, Caryl E.; Velaz, Laly (East Carolina University, 1992-08-15)
    Cleavage of caldesmon with chymotrypsin yields a series of fragments which bind both calmodulin and actin and inhibit the binding of myosin subfragments to actin and the subsequent stimulation of ATPase activity. Several ...

  • A Long Helix from the Central Region of Smooth Muscle Caldesmon 

    Wang, C.-L. Albert; Chalovich, Joseph; Graceffa, Philip; Lu, Renne C.; Mabuchi, Katsuhide; Stafford, Walter F. (East Carolina University, 1991-07-25)
    The central region of smooth muscle caldesmon is predicted to form α-helices on the basis of its primary structure. We have isolated a fragment (CT54) that contains this region. The hydrodynamic properties and the electron ...