Author | Chalovich, Joseph | en_US |
Author | Chen, Yi-Der | en_US |
Author | Dudek, Ronald W. | en_US |
Author | Hai, Luo | en_US |
Date Accessioned | 2010-11-08T19:07:55Z | en_US |
Date Accessioned | 2011-05-17T01:27:03Z | |
Date Available | 2010-11-08T19:07:55Z | en_US |
Date Available | 2011-05-17T01:27:03Z | |
Date of Issue | 1995-04-28 | en_US |
Identifier (Citation) | Journal of Biological Chemistry; 270:17 p. 9911-9916 | en_US |
Identifier (URI) | http://hdl.handle.net/10342/2994 | en_US |
Description | The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan actin. The concentration dependence of the observed rate of caldesmon-actin binding was analyzed to a first approximation as a single-step reaction using a Monte Carlo simulation. The derived association and dissociation rates were 107 M-1 s-1 and 18.2 s-1, respectively. Smooth muscle tropomyosin enhances the binding of caldesmon to actin, and this was found to be due to a reduction in the rate of dissociation to 6.3 s -1. There is no evidence from this study for a different mechanism of binding in the presence of tropomyosin. The fluorescence changes that occurred with the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or actin-tropomyosin were reversed by the addition of myosin subfragment 1 as predicted by a competitive binding mechanism. Originally published in the Journal of Biological Chemistry, Vol. 270, No. 17, 1995. | en_US |
Language | en_US | en_US |
Publisher | East Carolina University | en_US |
Related URI | http://www.jbc.org/search?author1=&fulltext=&pubdate_year=1995&volume=270&firstpage=9911&submit=yes | en_US |
Rights | Permission granted by author to Kent Nixon Myers to upload this article on 10/03/2010. | en_US |
Subject | Caldesmon interactions | en_US |
Subject | Flourescence changes in labeled molecules | en_US |
Subject | Actin binding | en_US |
Title | Kinetics of Binding of Caldesmon to Actin | en_US |
Type | Article | en_US |
Identifier (PMID) | PMC1262633 | en_US |
Identifier (DOI) | 10.1074/jbc.270.17.9911 | |