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Author | Fooksman, David Robert | en_US |
Author | Shaikh, Saame Raza | en_US |
Author | Boyle, Sarah | en_US |
Author | Edidin, Michael | en_US |
Date Accessioned | 2011-03-02T15:32:51Z | en_US |
Date Accessioned | 2011-05-17T01:40:06Z | |
Date Available | 2011-03-02T15:32:51Z | en_US |
Date Available | 2011-05-17T01:40:06Z | |
Date of Issue | 2009-05-01 | en_US |
Identifier (Citation) | Journal of Immunology; 182:9 p. 5179-5182 | en_US |
Identifier (URI) | http://hdl.handle.net/10342/3276 | en_US |
Description | Little is known about the signaling that occurs in an antigen presenting cell (APC) during contact with a T cell. Here we report the concentration of the signaling lipid, PI(4,5)P2, at the APC side of the immunological synapse. In both human and mouse cells, a PI(4,5)P2-specific fluorescent reporter, PH-GFP, detected an antigen-dependent enrichment of PI(4,5)P2 at the synapse between antigen- specific T cells and APC. When PIP(4,5)P2 was sequestered by a high concentration of PH-GFP reporter, cells were less susceptible to CTL-mediated lysis than control cells. These findings suggest a new regulatory target for modulating immune function that may be exploited for immune escape by pathogens and tumors. Originally published Journal of Immunology, Vol. 182, No. 9, May 2009 | en_US |
Language | en_US | en_US |
Publisher | East Carolina University | en_US |
Related URI | http://www.jimmunol.org/content/182/9/5179.long | en_US |
Rights | Author notified of opt-out rights by Cammie Jennings. | en_US |
Subject | Cytotoxicity | en_US |
Subject | T cells cytotoxic | en_US |
Subject | MHC | en_US |
Subject | Antigen Presentation | en_US |
Title | PI(4,5)P2 concentration at the APC side of the Immunological Synapse is Required for Effector T cell Function | en_US |
Type | Article | en_US |
Identifier (PMID) | PMC2799928 | en_US |