Author | Huang, Shu-shi | en_US |
Author | Chen, De | en_US |
Author | Pelczar, Patricia L. | en_US |
Author | Vepachedu, Venkata Ramana | en_US |
Author | Setlow, Peter | en_US |
Author | Li, Yong-Qing | en_US |
Date Accessioned | 2011-03-02T15:42:16Z | en_US |
Date Accessioned | 2011-05-17T15:07:53Z | |
Date Available | 2011-03-02T15:42:16Z | en_US |
Date Available | 2011-05-17T15:07:53Z | |
Date of Issue | 2007-07 | en_US |
Identifier (Citation) | Journal of Bacteriology; 189:13 p. 4681-4687 | en_US |
Identifier (URI) | http://hdl.handle.net/10342/3281 | en_US |
Description | Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many environmental stresses and in spore stability, and Ca-DPA levels in spore populations can vary with spore species/strains, as well as with sporulation conditions. We have measured levels of Ca-DPA in large numbers of individual spores in populations of a variety of Bacillus species and strains by using microfluidic Raman tweezers, in which a single spore is trapped in a focused laser beam and its Ca-DPA is quantitated from the intensity of the Ca-DPA-specific band at 1,017 cm!1 in Raman spectroscopy. Conclusions from these measurements include the following: (i) Ca-DPA concentrations in the spore core are >800 mM, well above Ca-DPA solubility; (ii) SpoVA proteins may be involved in Ca-DPA uptake in sporulation; and (iii) Ca-DPA levels differ significantly among individual spores in a population, but much of this variation could be due to variations in the sizes of individual spores. Originally published Journal of Bacteriology, Vol. 189, No. 13, July 2007 | en_US |
Language | en_US | en_US |
Publisher | East Carolina University | en_US |
Related URI | http://jb.asm.org/cgi/content/abstract/189/13/4681 | en_US |
Rights | Author notified of opt-out rights by Cammie Jennings. | en_US |
Subject | Bacillus spores | en_US |
Subject | Ca-DPA | en_US |
Subject | Raman spectroscopy | en_US |
Title | Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers | en_US |
Type | Article | en_US |
Identifier (PMID) | PMC1913426 | en_US |
Identifier (DOI) | 10.1128/JB.00282-07 | |