Author | Rogers, Marc B. | en_US |
Author | Parker, Anita C. | en_US |
Author | Smith, C. Jeffrey | en_US |
Date Accessioned | 2011-04-28T14:28:12Z | en_US |
Date Accessioned | 2011-05-17T01:40:09Z | |
Date Available | 2011-04-28T14:28:12Z | en_US |
Date Available | 2011-05-17T01:40:09Z | |
Date of Issue | 1993-11 | en_US |
Identifier (Citation) | Antimicrobial Agents and Chemotherapy; 37:11 p. 2391-2400 | en_US |
Identifier (URI) | http://hdl.handle.net/10342/3372 | en_US |
Description | Bacteroides frgiglis CS30 is a clinical isolate resistant to high concentrations of benzylpenicillin and cephaloridine but not to cephamycin or penem antibiotics. beta-Lactam resistance is mediated by a chromosomally encoded cephalosporinase produced at a high level. The gene encoding this beta-lactamase was cloned from genomic libraries constructed in Escherichia coli and then mated with B. fragilis 638 for identification of ampicillin-resistant (Apr) strains. Apr transconjugants contained a nitrocefin-reactive protein with the physical and enzymatic properties of the original CS30 isolate. The beta-lactamase gene (cepA) was localized by deletion analysis and subcloned, and its nucleotide sequence was determined. The 903-bp cepA open reading frame encoded a 300-amino-acid precursor protein (predicted molecular mass, 34,070 Da). A 13-lactamase-deficient mutant strain of B. fiugilis 638 was constructed by insertional inactivation with the cepA gene of CS30, demonstrating strict functional homology between these chromosomal beta-lactamase genes. An extensive comparison of the CepA protein sequence by alignment with other beta-lactamases revealed the strict conservation of at least four elements common to Ambler class A. A further comparison of the CepA protein sequence with protein sequences of beta-lactamases from two other Bacteroides species indicated that they constitute their own distinct subgroup of class A beta-lactamases. Originally published Antimicrobial Agents and Chemotherapy, Vol. 37, No. 11, Nov 1993 | en_US |
Language | en_US | en_US |
Publisher | East Carolina University | en_US |
Related URI | http://aac.asm.org/archive/1993.dtl | en_US |
Rights | Author notified of opt-out rights by Cammie Jennings prior to upload of this article. | en_US |
Subject | Bacteroides fragilis | en_US |
Subject | Beta-lactamase | en_US |
Subject | Cephalosporinase | en_US |
Title | Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases. | en_US |
Type | Article | en_US |
Identifier (PMID) | PMC192397 | en_US |
Identifier (DOI) | 10.1128/AAC.37.11.2391 | |
Journal Name | Antimicrobial Agents and Chemotherapy | |
Journal Volume | 37 | |
Journal Issue | 11 | |
Article Pages | 2391-2400 | |