Advisor | Christensen, Tim | en_US |
Author | Gosnell, Justin A. | en_US |
Date Accessioned | 2011-06-24T15:32:02Z | |
Date Available | 2011-06-24T15:32:02Z | |
Date of Issue | 2010 | en_US |
Identifier (URI) | http://hdl.handle.net/10342/3560 | |
Description | Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding against DNA damage. The Ctf4 protein is a central member of the replication fork and links the replicative MCM helicase and polymerase [alpha]-primase. In addition, it has been implicated as a member of a complex that promotes replication fork stability, the Fork Protection Complex (FPC). This investigation represents the first phenotypic analysis of the function of the Ctf4 protein within a multicellular organism model. We show that Ctf4 interacts with Polymerase [alpha], MCM2, Psf1, and Psf2. We also demonstrate that knockdown of this central replication fork component via a GAL4-UAS RNAi system results in a lower frequency of mitosis due to an S-phase delay, endoreplication defects, as well as mitotic bridging in early embryonic development. | en_US |
Extent | 56 p. | en_US |
Format Medium | dissertations, academic | en_US |
Language | | en_US |
Publisher | East Carolina University | en_US |
Subject | Biology, Molecular | en_US |
Subject | Genetics | en_US |
Subject | Biology | en_US |
Subject | Ctf4 | en_US |
Subject | Endoreplication | en_US |
Subject | FPC | en_US |
Subject | Biology, Genetics | |
Subject | Molecular biology | |
Library of Congress Subject Headings | DNA polymerases | |
Library of Congress Subject Headings | Protein binding | |
Library of Congress Subject Headings | DNA replication | |
Library of Congress Subject Headings | Cell cycle | |
Library of Congress Subject Headings | Eukaryotic cells | |
Library of Congress Subject Headings | Genomes | |
Library of Congress Subject Headings | Drosophila | |
Title | Drosophila Ctf4 is essential for genome stability and normal cell cycle progression | en_US |
Alternative Title | Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression | |
Type | Master's Thesis | en_US |
Department | Biology: Molecular Biology and Biotechnology | en_US |
Degree | M.S. | en_US |