Browsing Microbiology and Immunology by Issue Date
Now showing items 1-20 of 268
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Human T-cell Leukemia Virus Type 1 Basic Leucine Zipper Factor (HBZ) Interacts and Inhibits the Acetyltransferase Activity of Multiple Cellular Coactivator Families to Deregulate Transcription
(East Carolina University, 1/13/16)Of the 20 million individuals infected worldwide with the complex retrovirus, Human T-cell Leukemia Virus Type 1, 5% will develop an incurable and fatal form of leukemia known as adult T-cell leukemia (ATL). During the ... -
Butyl Rubber Stoppers Increase the Shelf Life of Prereduced, Anaerobically Sterilized Media
(East Carolina University, 1977-05)Butyl rubber stoppers as compared with neoprene or black rubber stoppers significantly increased the shelf life of prereduced, anaerobically sterilized media for growth of obligately anaerobic bacteria. Originally published ... -
Cecal and fecal bacterial flora of the Mongolian gerbil and the chinchilla.
(East Carolina University, 1988-05)The Mongolian gerbil is being increasingly used as a laboratory animal and as a pet. Both chinchillas and gerbils are used as animal models for otitis media and other otic research. Previously, only incomplete information ... -
Effects of palindromes on in vivo DNA replication and mutagenesis in bacteriophage phi X174 RF DNA.
(East Carolina University, 1988-11-25)Bacteriophage IX174 mutants with insertions of palindromic DNA sequences are rapidly outgrown by competing wild-type phage (Muller & Turnage, J. Mol. Biol. 189: 285). The basis for this defect was investigated and found ... -
Absence of unsaturated fatty acid synthesis in murine T lymphocytes.
(East Carolina University, 1989-08)Stearic acid is toxic for T lymphocytes in vitro but has little effect on B lymphocytes. To investigate the molecular basis for this difference, purified murine T and B lymphocytes were compared for their abilities to ... -
Effect of in vitro transcription on cruciform stability.
(East Carolina University, 1990-05-11)We have investigated the effect of in vitro transcription on cruciform stability. Replicative form DNA of 4X174 strain ins6240, containing a 48 bp synthetic palindrome in the J-F intercistronic region, was supercoiled in ... -
Molecular basis for the immunosuppressive action of stearic acid on T cells.
(East Carolina University, 1990-07)Studies were performed to determine the mechanism by which stearic acid (18: 0) selectively inhibits T-dependent immune responses in vitro. Incubation of mitogen-activated B and T cells with 18:0 resulted in dissimilar ... -
Nucleotide sequence of the phosphoprotein (P) gene of Newcastle disease virus (strain Beaudette C).
(East Carolina University, 1992-02) -
Analysis of rRNA restriction fragment length polymorphisms from Bacteroides spp. and Bacteroides fragilis isolates associated with diarrhea in humans and animals.
(East Carolina University, 1992-04)The Escherichia coli rRNA operon rrnB was used as a 32P-labeled hybridization probe in Southern blots of genomic DNAs from representative strains of the saccharolytic, gram-negative, obligate anaerobes of the genus Bacteroides. ... -
Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.
(East Carolina University, 1993-02)Iron acquisition by the gram-negative pathogens Bordetella bronchiseptica and Bordetella pertussis is thought to occur by hydroxamate siderophore-mediated transport as well as an apparently siderophore-independent process ... -
Identification of a circular intermediate in the transfer and transposition of Tn4555, a mobilizable transposon from Bacteroides spp.
(East Carolina University, 1993-05)Transmissible cefoxitin (FX) resistance in Bacteroides vulgatus CLA341 was associated with the 12.5-kb, mobilizable transposon, Tn4555, which encoded the 13-lactamase gene cfxA. Transfer occurred by a conjugation-like ... -
Genetic and biochemical analysis of a novel Ambler class A β-Lactamase responsible for cefoxitin resistance in Bacteroides species.
(East Carolina University, 1993-05)A clinical isolate of Bacteroides vulgatus was resistant to tetracycline, clindamycin, ampicillin, cephaloridine, cefoxitin, and other 13-lactam antibiotics except imipenem. 13-Lactam resistance was mediated by a ... -
Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium.
(East Carolina University, 1993-05-15)CCRF-CEM is a human T-cell line originally isolated from a child with acute lymphoblastic leukemia. At cell densities > 2 x 10 cells per ml, CEM cells grow in serum-free medium, but at lower cell densities the cultures ... -
Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.
(East Carolina University, 1993-11)Bacteroides frgiglis CS30 is a clinical isolate resistant to high concentrations of benzylpenicillin and cephaloridine but not to cephamycin or penem antibiotics. beta-Lactam resistance is mediated by a chromosomally encoded ... -
Insertional activation of cepA leads to high-level beta-lactamase expression in Bacteroides fragilis clinical isolates.
(East Carolina University, 1994-07)Bacteroides fragilis is an important opportunistic pathogen of humans and is resistant to many drugs commonly used to treat anaerobic infections, including P-lactams. A strain set comprised of B. fragilis isolates producing ... -
Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.
(East Carolina University, 1994-08)The gene, cbl, encoding the species-specific, clavulanate-susceptible, endogenous cephalosporinase was cloned from Bacteroides uniforinis WAL-7088. The nucleotide sequence was determined, and the cbl structural gene was ... -
Bordetella pertussis fur gene restores iron repressibility of siderophore and protein expression to deregulated Bordetella bronchiseptica mutants.
(East Carolina University, 1995-01)We report the isolation and preliminary phenotypic characterization of manganese-resistant Bordetella bronchiseptica mutants with respect to deregulation of siderophore and iron-regulated protein expression. The fur gene ... -
Biochemical and genetic analyses of a catalase from the anaerobic bacterium Bacteroides fragilis.
(East Carolina University, 1995-06)A single catalase enzyme was produced by the anaerobic bacterium Bacteroides fragilis when cultures at late log phase were shifted to aerobic conditions. In anaerobic conditions, catalase activity was detected ... -
Cloning and characterization of a conjugated bile acid hydrolase gene from Clostridium perfringens.
(East Carolina University, 1995-07)The gene encoding a conjugated bile acid hydrolase (CBAH) from Clostridium perfringens 13 has been cloned and expressed in Escherichia coli, and its nucleotide sequence has been determined. Nucleotide and predicted amino ... -
Characterization of the avian GLUT1 glucose transporter: differential regulation of GLUT1 and GLUT3 in chicken embryo fibroblasts.
(East Carolina University, 1995-11)Vertebrate cells that are transformed by oncogenes such as v-src or are stimulated by mitogens have increased rates of glucose uptake. In rodent cels, the mechanisms whereby glucose transport is up-regulated are well ...