Selection of Reliable Reference Genes in for Analysis of Nanotoxicity
Zhang, Yanqiong; Chen, Dongliang; Smith, Michael A.; Zhang, Baohong; Pan, Xiaoping
Despite rapid development and application of a wide range of manufactured metal oxide nanoparticles (NPs), the understanding of potential risks of using NPs is less completed, especially at the molecular level. The nematode Caenorhabditis elegans (C.elegans) has been emerging as an environmental model to study the molecular mechanism of environmental contaminations, using standard genetic tools such as the real-time quantitative PCR (RT-qPCR). The most important factor that may affect the accuracy of RT-qPCR is to choose appropriate genes for normalization. In this study, we selected 13 reference gene candidates (act-1, cdc-42, pmp-3, eif-3.C, actin, act-2, csq-1, Y45F10D.4, tba-1, mdh-1, ama-1, F35G12.2, and rbd-1) to test their expression stability under different doses of nano-copper oxide (CuO 0, 1, 10, and 50 µg/mL) using RT-qPCR. Four algorithms, geNorm, NormFinder, BestKeeper, and the comparative ΔCt method, were employed to evaluate these 13 candidates expressions. As a result, tba-1, Y45F10D.4 and pmp-3 were the most reliable, which may be used as reference genes in future study of nanoparticle-induced genetic response using C.elegans.
Zhang, Yanqiong, & Chen, Dongliang, & Smith, Michael A., & Zhang, Baohong, & Pan, Xiaoping. (January 2012). Selection of Reliable Reference Genes in for Analysis of Nanotoxicity. PLoS ONE, 7(3), 1- 7. Retrieved from http://hdl.handle.net/10342/5480
Zhang, Yanqiong, and Chen, Dongliang, and Smith, Michael A., and Zhang, Baohong, and Pan, Xiaoping. "Selection of Reliable Reference Genes in for Analysis of Nanotoxicity". PLoS ONE. 7:3. (1-7), January 2012. August 16, 2018. http://hdl.handle.net/10342/5480.
Zhang, Yanqiong and Chen, Dongliang and Smith, Michael A. and Zhang, Baohong and Pan, Xiaoping, "Selection of Reliable Reference Genes in for Analysis of Nanotoxicity," PLoS ONE 7, no. 3 (January 2012), http://hdl.handle.net/10342/5480 (accessed August 16, 2018).
Zhang, Yanqiong, Chen, Dongliang, Smith, Michael A., Zhang, Baohong, Pan, Xiaoping. Selection of Reliable Reference Genes in for Analysis of Nanotoxicity. PLoS ONE. January 2012; 7(3): 1-7. http://hdl.handle.net/10342/5480. Accessed August 16, 2018.