Automatic quantitative analysis of morphology of apoptotic HL-60 cells
Liu, Yahui; Lin, Wang; Yang, Xu; Liang, Weizi; Zhang, Jun; Meng, Maobin; Rice, John R.; Sa, Yu; Feng, Yuanming
Morphological identification is a widespread procedure to assess the presence of apoptosis by visual inspection of the morphological characteristics or the fluorescence images. The procedure is lengthy and results are observer dependent. A quantitative automatic analysis is objective and would greatly help the routine work. We developed an image processing and segmentation method which combined the Otsu thresholding and morphological operators for apoptosis study. An automatic determination method of apoptotic stages of HL-60 cells with fluorescence images was developed. Comparison was made between normal cells, early apoptotic cells and late apoptotic cells about their geometric parameters which were defined to describe the features of cell morphology. The results demonstrated that the parameters we chose are very representative of the morphological characteristics of apoptotic cells. Significant differences exist between the cells in different stages, and automatic quantification of the differences can be achieved.
Liu, Yahui, & Lin, Wang, & Yang, Xu, & Liang, Weizi, & Zhang, Jun, & Meng, Maobin, & Rice, John R., & Sa, Yu, & Feng, Yuanming. (January 2014). Automatic quantitative analysis of morphology of apoptotic HL-60 cells. EXCLI Journal, 13(), 19- 27. Retrieved from http://hdl.handle.net/10342/5730
Liu, Yahui, and Lin, Wang, and Yang, Xu, and Liang, Weizi, and Zhang, Jun, and Meng, Maobin, and Rice, John R., and Sa, Yu, and Feng, Yuanming. "Automatic quantitative analysis of morphology of apoptotic HL-60 cells". EXCLI Journal. 13:. (19-27), January 2014. December 11, 2018. http://hdl.handle.net/10342/5730.
Liu, Yahui and Lin, Wang and Yang, Xu and Liang, Weizi and Zhang, Jun and Meng, Maobin and Rice, John R. and Sa, Yu and Feng, Yuanming, "Automatic quantitative analysis of morphology of apoptotic HL-60 cells," EXCLI Journal 13, no. (January 2014), http://hdl.handle.net/10342/5730 (accessed December 11, 2018).
Liu, Yahui, Lin, Wang, Yang, Xu, Liang, Weizi, Zhang, Jun, Meng, Maobin, Rice, John R., Sa, Yu, Feng, Yuanming. Automatic quantitative analysis of morphology of apoptotic HL-60 cells. EXCLI Journal. January 2014; 13(): 19-27. http://hdl.handle.net/10342/5730. Accessed December 11, 2018.