In Vivo Blood Glucose Quantification Using Raman Spectroscopy
Shao, Jingwei; Lin, Manman; Li, Yong-Qing; Li, Xue; Liu, Junxian; Liang, Jianpin; Yao, Huilu
We here propose a novel Raman spectroscopy method that permits the noninvasive measurement of blood glucose concentration. To reduce the effects of the strong background signals produced by surrounding tissue and to obtain the fingerprint Raman lines formed by blood analytes, a laser was focused on the blood in vessels in the skin. The Raman spectra were collected transcutaneously. Characteristic peaks of glucose (1125 cm-1) and hemoglobin (1549 cm-1) were observed. Hemoglobin concentration served as an internal standard, and the ratio of the peaks that appeared at 1125 cm-1 and 1549 cm-1 peaks was used to calculate the concentration of blood glucose. We studied three mouse subjects whose blood glucose levels became elevated over a period of 2 hours using a glucose test assay. During the test, 25 Raman spectra were collected transcutaneously and glucose reference values were provided by a blood glucose meter. Results clearly showed the relationship between Raman intensity and concentration. The release curves were approximately linear with a correlation coefficient of 0.91. This noninvasive methodology may be useful for the study of blood glucose in vivo.
Shao, Jingwei, & Lin, Manman, & Li, Yong-Qing, & Li, Xue, & Liu, Junxian, & Liang, Jianpin, & Yao, Huilu. (January 2012). In Vivo Blood Glucose Quantification Using Raman Spectroscopy. PLoS ONE, 7(10), 1- 6. Retrieved from http://hdl.handle.net/10342/5772
Shao, Jingwei, and Lin, Manman, and Li, Yong-Qing, and Li, Xue, and Liu, Junxian, and Liang, Jianpin, and Yao, Huilu. "In Vivo Blood Glucose Quantification Using Raman Spectroscopy". PLoS ONE. 7:10. (1-6), January 2012. May 20, 2019. http://hdl.handle.net/10342/5772.
Shao, Jingwei and Lin, Manman and Li, Yong-Qing and Li, Xue and Liu, Junxian and Liang, Jianpin and Yao, Huilu, "In Vivo Blood Glucose Quantification Using Raman Spectroscopy," PLoS ONE 7, no. 10 (January 2012), http://hdl.handle.net/10342/5772 (accessed May 20, 2019).
Shao, Jingwei, Lin, Manman, Li, Yong-Qing, Li, Xue, Liu, Junxian, Liang, Jianpin, Yao, Huilu. In Vivo Blood Glucose Quantification Using Raman Spectroscopy. PLoS ONE. January 2012; 7(10): 1-6. http://hdl.handle.net/10342/5772. Accessed May 20, 2019.