Expansion of cardiac ischemia/reperfusion injury after instillation of three forms of multi-walled carbon nanotubes
Author
Urankar, Rakhee N.; Lust, Robert M.; Mann, Erin; Katwa, Pranita; Wang, Xiaojia; Podila, Ramakrishna; Hilderbrand, Susana C.; Harrison, Benjamin S.; Chen, Pengyu; Ke, Pu Chun; Rao, Apparao M.; Brown, Jared; Wingard, Christopher J.
Abstract
Background
The exceptional physical-chemical properties of carbon nanotubes have lead to their use in diverse commercial and biomedical applications. However, their utilization has raised concerns about human exposure that may predispose individuals to adverse health risks. The present study investigated the susceptibility to cardiac ischemic injury following a single exposure to various forms of multi-walled carbon nanotubes (MWCNTs). It was hypothesized that oropharyngeal aspiration of MWCNTs exacerbates myocardial ischemia and reperfusion injury (I/R injury).
Methods
Oropharyngeal aspiration was performed on male C57BL/6J mice with a single amount of MWCNT (0.01 - 100 μg) suspended in 100 μL of a surfactant saline (SS) solution. Three forms of MWCNTs were used in this study: unmodified, commercial grade (C-grade), and functionalized forms that were modified either by acid treatment (carboxylated, COOH) or nitrogenation (N-doped) and a SS vehicle. The pulmonary inflammation, serum cytokine profile and cardiac ischemic/reperfusion (I/R) injury were assessed at 1, 7 and 28 days post-aspiration.
Results
Pulmonary response to MWCNT oropharyngeal aspiration assessed by bronchoalveolar lavage fluid (BALF) revealed modest increases in protein and inflammatory cell recruitment. Lung histology showed modest tissue inflammation as compared to the SS group. Serum levels of eotaxin were significantly elevated in the carboxylated MWCNT aspirated mice 1 day post exposure. Oropharyngeal aspiration of all three forms of MWCNTs resulted in a time and/or dose-dependent exacerbation of myocardial infarction. The severity of myocardial injury varied with the form of MWCNTs used. The N-doped MWCNT produced the greatest expansion of the infarct at any time point and required a log concentration lower to establish a no effect level. The expansion of the I/R injury remained significantly elevated at 28 days following aspiration of the COOH and N-doped forms, but not the C-grade as compared to SS.
Conclusion
Our results suggest that oropharyngeal aspiration of MWCNT promotes increased susceptibility of cardiac tissue to ischemia/reperfusion injury without a significant pulmonary inflammatory response. The cardiac injury effects were observed at low concentrations of MWCNTs and presence of MWCNTs may pose a significant risk to the cardiovascular system.
Date
2012
Citation:
APA:
Urankar, Rakhee N., & Lust, Robert M., & Mann, Erin, & Katwa, Pranita, & Wang, Xiaojia, & Podila, Ramakrishna, & Hilderbrand, Susana C., & Harrison, Benjamin S., & Chen, Pengyu, & Ke, Pu Chun, & Rao, Apparao M., & Brown, Jared, & Wingard, Christopher J.. (January 2012).
Expansion of cardiac ischemia/reperfusion injury after instillation of three forms of multi-walled carbon nanotubes.
Particle and Fibre Toxicology,
9(),
38-
38. Retrieved from
http://hdl.handle.net/10342/5792
MLA:
Urankar, Rakhee N., and Lust, Robert M., and Mann, Erin, and Katwa, Pranita, and Wang, Xiaojia, and Podila, Ramakrishna, and Hilderbrand, Susana C., and Harrison, Benjamin S., and Chen, Pengyu, and Ke, Pu Chun, and Rao, Apparao M., and Brown, Jared, and Wingard, Christopher J..
"Expansion of cardiac ischemia/reperfusion injury after instillation of three forms of multi-walled carbon nanotubes". Particle and Fibre Toxicology.
9:. (38-38),
January 2012.
April 19, 2024.
http://hdl.handle.net/10342/5792.
Chicago:
Urankar, Rakhee N. and Lust, Robert M. and Mann, Erin and Katwa, Pranita and Wang, Xiaojia and Podila, Ramakrishna and Hilderbrand, Susana C. and Harrison, Benjamin S. and Chen, Pengyu and Ke, Pu Chun and Rao, Apparao M. and Brown, Jared and Wingard, Christopher J.,
"Expansion of cardiac ischemia/reperfusion injury after instillation of three forms of multi-walled carbon nanotubes," Particle and Fibre Toxicology 9, no.
(January 2012),
http://hdl.handle.net/10342/5792 (accessed
April 19, 2024).
AMA:
Urankar, Rakhee N., Lust, Robert M., Mann, Erin, Katwa, Pranita, Wang, Xiaojia, Podila, Ramakrishna, Hilderbrand, Susana C., Harrison, Benjamin S., Chen, Pengyu, Ke, Pu Chun, Rao, Apparao M., Brown, Jared, Wingard, Christopher J..
Expansion of cardiac ischemia/reperfusion injury after instillation of three forms of multi-walled carbon nanotubes. Particle and Fibre Toxicology.
January 2012;
9():
38-38.
http://hdl.handle.net/10342/5792. Accessed
April 19, 2024.
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