Microsatellite analysis of brook trout, Salvelinus fontinalis, in western Pennsylvania
Horiates, Julia M.
In western Pennsylvania, the brook trout, Salvelinus fontinalis, has dramatically declined in population due to the environmental stressors of mining, logging, water withdrawal, and acid deposition. Brook trout are game fish and are valued economically in this region which makes their declining populations concerning. To better understand brook trout population dynamics in western Pennsylvania, we conducted a genetic analysis to determine patterns of population connectivity and genetic diversity. Microsatellites are short repeat sequences within the genome, and are highly susceptible to mutation making them suitable as neutral markers for analyzing patterns of regional population variation. Therefore, we conducted a microsatellite analysis of 16 individuals from 4 streams, for a total of 64 samples. Seven tri-and tetranucleotide microsatellite DNA markers were analyzed to investigate allelic diversity of brook trout. Amplified microsatellite loci were analyzed using Geneious 6.0 software that estimated PCR product sizes and converted sizes into estimates of the number of microsatellite repeats at each locus. Genepop 4.2 program was used to obtain the fixation index (FST) to measure levels of population differentiation and connectivity. The results showed moderate divergence (average pairwise divergence FST= 0.24; range of 0.06-0.30) with highly significant differentiation (P < 0.001). My study suggests that even populations that are spatially close to one another show evidence of divergence. This finding suggests limited dispersal between streams and will inform species management strategies for this species.
Horiates, Julia M.. (May 2017). Microsatellite analysis of brook trout, Salvelinus fontinalis, in western Pennsylvania (Honors Thesis, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/6261.)
Horiates, Julia M.. Microsatellite analysis of brook trout, Salvelinus fontinalis, in western Pennsylvania. Honors Thesis. East Carolina University, May 2017. The Scholarship. http://hdl.handle.net/10342/6261. October 17, 2018.
Horiates, Julia M., “Microsatellite analysis of brook trout, Salvelinus fontinalis, in western Pennsylvania” (Honors Thesis., East Carolina University, May 2017).
Horiates, Julia M.. Microsatellite analysis of brook trout, Salvelinus fontinalis, in western Pennsylvania [Honors Thesis]. Greenville, NC: East Carolina University; May 2017.
East Carolina University
Showing items related by title, author, creator and subject.
Etheridge, Sherer Brooke (East Carolina University, 2015)Juncus roemerianus, black needlerush, is common in high marshes and occasionally in low marshes along the Mid-Atlantic and southern USA. Previous work found that J. roemerianus patches remained relatively stable in the ...
Surfactant Assisted Electrospray Ionization Time of Flight Mass Spectrometry of Synthetic Polymers Brooks, James L. (East Carolina University, 2010)Synthetic polymers are complex mixtures which often require extensive characterization to determine chemical composition and structure. Mass spectrometric techniques have greatly enhanced the quantity, as well as the ...
Tolar, Elizabeth Brooke (East Carolina University, 2010)The purpose of this thesis is to examine the commercial tobacco production of Virginia and Barbados and explore the connections between them. The economic activities, especially tobacco production, that developed altered ...