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Superdormant Spores of Bacillus Species Have Elevated Wet-Heat Resistance and Temperature Requirements for Heat Activation
(East Carolina University, 2009-09)
Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, D-glucose, or L-valine, respectively, germinate ...
The high concentration of Arg213 → Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities
(East Carolina University, 2005-01-15)
The C-terminal region of EC-SOD (extracellular superoxide dismutase) mediates the binding to both heparin/heparan sulphate and type I collagen. A mutation (Arg213→Gly; R213G) within this extracellular matrix-binding region ...
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis
(East Carolina University, 2008-07)
Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these ...
Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics
(East Carolina University, 2009-05-15)
Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneously measure levels of Ca-dipicolinic acid (CaDPA) and changes in spore morphology and refractive index during germination ...
Electron Emission from Foils and Biological Materials after Proton Impact
(East Carolina University, 2008-10)
Electron emission spectra from thin metal foils with thin layers of water frozen on them (amorphous solid water) after fast proton impact have been measured and have been simulated in liquid water using the event-by-event ...
Quantifying Double-Strand Breaks and Clustered Damages in DNA by Single-Molecule Laser Fluorescence Sizing
(East Carolina University, 2003-02)
Fluorescence from a single DNA molecule passing through a laser beam is proportional to the size (contour length) of the molecule, and molecules of different sizes can be counted with equal efficiencies. Single-molecule ...
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers
(East Carolina University, 2007-07)
Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many ...
Interaction cross sections needed for simulation of secondary electron emission spectra from thin metal foils after fast proton impact
(East Carolina University, 2009)
Monte Carlo simulations of secondary electron emission from thin metal foils after fast proton impact require reliable interaction cross sections with the target under consideration. Total and energy differential inelastic ...
Flow cytometer apparatus for three dimensional diffraction imaging and related methods
(2009-12-17)
A flow cytometer assembly includes a fluid controller configured to form a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid. A coherent light source is configured to illuminate ...
How Moist Heat Kills Spores of Bacillus subtilis
(East Carolina University, 2007-12)
Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores ...