2024-03-28T12:10:30Zhttps://thescholarship.ecu.edu/oai/requestoai:TheScholarship.intra.ecu.edu:10342/57402021-03-03T21:11:14Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Nguyen, Nam P.
author
Vos, Paul
author
Vinh-Hung, Vincent
author
Ceizyk, Misty
author
Smith-Raymond, Lexie
author
Stevie, Michelle
author
Slane, Benjamin
author
Chi, Alexander
author
Desai, Anand
author
Krafft, Shane P.
author
Jang, Siyoung
author
Hamilton, Russ
author
Karlsson, Ulf
author
Abraham, Dave
author
2012
Background
To evaluate the feasibility of image-guided radiotherapy based on helical Tomotherapy to spare the contralateral parotid gland in head and neck cancer patients with unilateral or no neck node metastases.
Methods
A retrospective review of 52 patients undergoing radiotherapy for head and neck cancers with image guidance based on daily megavoltage CT imaging with helical tomotherapy was performed.
Results
Mean contralateral parotid dose and the volume of the contralateral parotid receiving 40 Gy or more were compared between radiotherapy plans with significant constraint (SC) of less than 20 Gy on parotid dose (23 patients) and the conventional constraint (CC) of 26 Gy (29 patients). All patients had PTV coverage of at least 95% to the contralateral elective neck nodes. Mean contralateral parotid dose was, respectively, 14.1 Gy and 24.7 Gy for the SC and CC plans (p < 0.0001). The volume of contralateral parotid receiving 40 Gy or more was respectively 5.3% and 18.2% (p < 0.0001)
Conclusion
Tomotherapy for head and neck cancer minimized radiotherapy dose to the contralateral parotid gland in patients undergoing elective node irradiation without sacrificing target coverage.
BMC Cancer; 12: p. 175-175
1471-2407
http://hdl.handle.net/10342/5740
pmc3411401
10.1186/1471-2407-12-175
Head and neck cancer
Tomotherapy
Parotid sparing
Feasibility of image-guided radiotherapy based on helical tomotherapy to reduce contralateral parotid dose in head and neck cancer
oai:TheScholarship.intra.ecu.edu:10342/30392021-03-03T20:54:56Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Lee, Tung-Kwang
author
O'Brien, Kevin F.
author
Wang, Weidong
author
Johnke, Roberta M.
author
Sheng, Chao
author
Benhabib, Sidi Mohammed
author
Wang, Tao
author
Allison, Ron R.
author
2010-05
Backgroundâ Ionizing radiation (IR) initiates intracellular oxidative stress through enhanced
formation of reactive oxygen species (ROS) that attack DNA leading to cell death. As the diversity
of IR applied in medicine, agriculture, industry, and the growing threats of global terrorism, the
acquisition of radioprotectors is an urgent need for the nation. However, the applicability of
radioprotectors currently under investigation is limited due to their inherent toxicity.
Objectiveâ This study investigated the effect of a standardized North American ginseng extract
(NAGE, total ginsenoside content: 11.7%) on DNA damage in human lymphocytes at 90 min postirradiation.
Designâ With the application of NAGE (250 â 1000 μg mlâ 1) at 90 min post-irradiation (1 and 2
Gy), DNA damage in lymphocytes obtained from 40 healthy individuals was evaluated by
cytokinesis-block micronucleus (CBMN) assay. Similar experiments were also performed in
lymphocytes treated with WR-1065 (1 mM or 3mM). In addition, before and after irradiation,
lymphocytes obtained from 10 individuals were measured for their total antioxidant capacity (TAC)
and the reactive oxygen species (ROS).
Resultsâ The significant effect of NAGE against 137Cs-induced MN in lymphocytes is
concentration-dependent. NAGE (750 μg mlâ 1) reduced MN yield by 50.7% after 1 Gy and 35.9%
after 2 Gy exposures, respectively; these results were comparable to that of WR-1065. Further, we
also found that NAGE reduces MN yield and ROS but increases TAC in lymphocytes.
Conclusionsâ Our results suggest that NAGE is a relatively non-toxic natural compound that
holds radioprotective potential in human lymphocytes even when applied at 90 min post-irradiation.
One of the radioprotective mechanisms may be mediated through the scavenging of free radicals and
enhancement of the intracellular TAC. Originally published Journal of Alternative and Complementary Medicine Vol. 16, No. 5 2010.
Journal of Alternative and Complementary Medicine; 16:5 p. 561-567
http://hdl.handle.net/10342/3039
PMC2876338
American ginseng
Radiation
Micronuclei
Lymphocytes
Oxidative stress
WR-1065
Radioprotective Effect of American Ginseng on Human Lymphocytes at 90 Minutes Post-irradiation: A Study of 40 Cases
oai:TheScholarship.intra.ecu.edu:10342/117912022-12-01T08:18:51Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Burke, Aidan M.
author
Carrasquilla, Michael
author
2022-01-12
2234-943X
http://hdl.handle.net/10342/11791
10.3389/fonc.2021.794615
brain metastases
immunotherapy
radiation necrosis
Volume of Disease as a Predictor for Clinical Outcomes in Patients With Melanoma Brain Metastases Treated With Stereotactic Radiosurgery and Immune Checkpoint Therapy
oai:TheScholarship.intra.ecu.edu:10342/57342022-12-09T19:10:05Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Seo, Hyun Won
author
Cheon, Se-Myeong
author
Lee, Myon-Hee
author
Kim, Hong Jun
author
Jeon, Hoon
author
Cha, Dong Seok
author
2015
Catalpol is an effective component of rehmannia root and known to possess various pharmacological properties. The present study was aimed at investigating the potential effects of catalpol on the lifespan and stress tolerance using C. elegans model system. Herein, catalpol showed potent lifespan extension of wild-type nematode under normal culture condition. In addition, survival rate of catalpol-fed nematodes was significantly elevated compared to untreated control under heat and oxidative stress but not under hyperosmolality conditions. We also found that elevated antioxidant enzyme activities and expressions of stress resistance proteins were attributed to catalpol-mediated increased stress tolerance of nematode. We further investigated whether catalpol’s longevity effect is related to aging-related factors including reproduction, food intake, and growth. Interestingly, catalpol exposure could attenuate pharyngeal pumping rate, indicating that catalpol may induce dietary restriction of nematode. Moreover, locomotory ability of aged nematode was significantly improved by catalpol treatment, while lipofuscin levels were attenuated, suggesting that catalpol may affect age-associated changes of nematode. Our mechanistic studies revealed that mek-1, daf-2, age-1, daf-16, and skn-1 are involved in catalpol-mediated longevity. These results indicate that catalpol extends lifespan and increases stress tolerance of C. elegans via DAF-16/FOXO and SKN-1/Nrf activation dependent on insulin/IGF signaling and JNK signaling.
Evidence-based Complementary and Alternative Medicine : eCAM; 2015: p. 1-10
1741-427X
http://hdl.handle.net/10342/5734
pmc4363898
10.1155/2015/524878
Catalpol Modulates Lifespan via DAF-16/FOXO and SKN-1/Nrf2 Activation in
oai:TheScholarship.intra.ecu.edu:10342/47542022-12-12T17:47:36Zcom_10342_7351com_10342_6421com_10342_74com_10342_73com_10342_1col_10342_7366col_10342_96col_10342_6408col_10342_7167
00925njm 22002777a 4500
dc
Yu, T G
author
Feng, Y
author
Feng, XY
author
Dai, J Z
author
Huang, Zhibin
author
2014-11-27
Objective:
To investigate the relationship between the tumour volume and metabolic rates of astrocytic tumours using MR spectroscopy (MRS) during radiation therapy (RT).
Methods:
12 healthy male Sprague-Dawley® rats (Sprague–Dawley Animal Company, Madison, WI) were used, and a tumour model was created through injecting C6 tumour cells into the right caudate nuclei of the rats. Tumours grew for 18 days after the injection and before the imaging study and radiation treatment. MRS was performed with two-dimensional multivoxel point-resolved spectroscopy sequence using a GE Signa VH/i 3.0-T MR scanner (GE Healthcare, Milwaukee, WI) equipped with rat-special coil. RT was given on the 19th day with a dose of 4 Gy in one single fraction. The image examinations were performed before RT, and on the 4th, 10th, 14th and 20th days after treatment, respectively. GE FuncTool software package (GE Healthcare) was used for post-processing of spectrum.
Results:
Metabolic ratios of serial MRS decrease progressively with time after RT. Choline-containing components (Cho)/creatine and creatine phosphate (Cr) ratios immediately prior to RT differed significantly from those on the 10th, 14th and 20th days after RT; both Cho/N-acetyl aspartate (NAA) ratios and NAA/Cr ratios immediately prior to RT differed significantly from those on the 14th and 20th days after RT. A positive correlation between changes of tumour volume and changes of Cho/Cr, lipid and lactate/Cr and glutamate plus glutamine/Cr ratio was observed on the 4th day after RT.
Conclusion:
MRS provides potential in monitoring tumour response during RT, and the imaging biomarkers predict the response of astrocytic tumours to treatment.
Advances in knowledge:
MRS is combined with both tumour size and Ki-67 labelling index to access tumour response to radiation.
The British Journal of Radiology; 88:1045 p. 1-10
http://hdl.handle.net/10342/4754
25382247
10.1259/bjr.20140418
Radiation therapy
Prognostic factor from MR spectroscopy in rat with astrocytic tumour during radiation therapy
oai:TheScholarship.intra.ecu.edu:10342/111082022-09-10T07:16:04Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Muzaffar, Mahvish
author
Navaid, Musharraf
author
Bulumulle, Anushi
author
Fessas, Petros
author
Naeem, Muntaha
author
Pinter, Matthias
author
Marron, Thomas U.
author
Szafron, David
author
2021-10-08
1664-5553
http://hdl.handle.net/10342/11108
10.1159/000519108
Cancer immunotherapy
Antibiotics
Hepatocellular carcinoma
Early Antibiotic Exposure Is Not Detrimental to Therapeutic Effect from Immunotherapy in Hepatocellular Carcinoma
oai:TheScholarship.intra.ecu.edu:10342/57272021-03-03T21:10:52Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Rana, Nitesh
author
Ju, Andrew Wenhua
author
Bazylewicz, Michael
author
Kallakury, Bhaskar
author
He, Aiwu Ruth
author
Unger, Keith R.
author
Lee, Justin S.
author
2013-11
Purpose: Yttrium-90 radioembolization (RE) is a locoregional therapy option for hepatocellular carcinoma (HCC). Sorafenib is a multikinase inhibitor used in HCC that can potentially affect the efficacy of RE by altering tumor vascularity or suppressing post-irradiation angiogenesis. The safety and efficacy of sorafenib followed by RE has not been previously reported.
Materials and Methods: Patients with HCC who received RE after sorafenib were included in this retrospective review. Overall survival, toxicity, and maximal radiographic response and necrosis criteria were examined.
Results: Ten patients (15 RE administrations) fit the inclusion criteria. All were Barcelona Clinic Liver Cancer (BCLC) stage C. Median follow-up was 16.5 weeks. Median overall survival and radiographic progression-free survival were 30 and 28 weeks, respectively. Significant differences in overall survival were seen based on Child-Pugh class (p = 0.002) and radiographic response (p = 0.009). Three patients had partial response, six had stable disease, and one had progressive disease. Grade 1 or 2 acute fatigue, anorexia, and abdominal pain were common. Three patients had Grade 3 ascites in the setting of disease progression. Two patients had Grade 3 biochemical toxicity. One patient was sufficiently downstaged following RE and sorafenib to receive a partial hepatectomy.
Conclusion: Yttrium-90 RE in patients with HCC who have received sorafenib demonstrate acceptable toxicity and rates of radiographic response. However, the overall survival is lower than that reported in the literature on RE alone or sorafenib alone. This may be due in part to more patients in this study having advanced disease compared to these other study populations. Larger prospective studies are needed to determine whether the combination of RE and sorafenib is superior to either therapy alone.
Frontiers in Oncology; 3: p. 1-10
2234-943X
http://hdl.handle.net/10342/5727
pmc3874555
10.3389/fonc.2013.00323
yttrium-90
radioembolization
Y90
HCC
hepatocellular carcinoma
sorafenib
SIRT
Yttrium-90 Radioembolization in Patients with Hepatocellular Carcinoma Who have Previously Received Sorafenib
oai:TheScholarship.intra.ecu.edu:10342/57242021-03-03T21:11:27Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Chen, Aishe
author
Dong, Lixue
author
Leffler, Nancy R.
author
Asch, Adam S.
author
Witte, Owen N.
author
Yang, Li V.
author
2011
Endothelium-leukocyte interaction is critical for inflammatory responses. Whereas the tissue microenvironments are often acidic at inflammatory sites, the mechanisms by which cells respond to acidosis are not well understood. Using molecular, cellular and biochemical approaches, we demonstrate that activation of GPR4, a proton-sensing G protein-coupled receptor, by isocapnic acidosis increases the adhesiveness of human umbilical vein endothelial cells (HUVECs) that express GPR4 endogenously. Acidosis in combination with GPR4 overexpression further augments HUVEC adhesion with U937 monocytes. In contrast, overexpression of a G protein signaling-defective DRY motif mutant (R115A) of GPR4 does not elicit any increase of HUVEC adhesion, indicating the requirement of G protein signaling. Downregulation of GPR4 expression by RNA interference reduces the acidosis-induced HUVEC adhesion. To delineate downstream pathways, we show that inhibition of adenylate cyclase by inhibitors, 2′,5′-dideoxyadenosine (DDA) or SQ 22536, attenuates acidosis/GPR4-induced HUVEC adhesion. Consistently, treatment with a cAMP analog or a Gi signaling inhibitor increases HUVEC adhesiveness, suggesting a role of the Gs/cAMP signaling in this process. We further show that the cAMP downstream effector Epac is important for acidosis/GPR4-induced cell adhesion. Moreover, activation of GPR4 by acidosis increases the expression of vascular adhesion molecules E-selectin, VCAM-1 and ICAM-1, which are functionally involved in acidosis/GPR4-mediated HUVEC adhesion. Similarly, hypercapnic acidosis can also activate GPR4 to stimulate HUVEC adhesion molecule expression and adhesiveness. These results suggest that acidosis/GPR4 signaling regulates endothelial cell adhesion mainly through the Gs/cAMP/Epac pathway and may play a role in the inflammatory response of vascular endothelial cells.
PLoS ONE; 6:11 p. 1-14
1932-6203
http://hdl.handle.net/10342/5724
pmc3217975
10.1371/journal.pone.0027586
Activation of GPR4 by Acidosis Increases Endothelial Cell Adhesion through the cAMP/Epac Pathway
oai:TheScholarship.intra.ecu.edu:10342/118062022-12-03T08:16:41Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Lee, Myon Hee
author
Namgong, Chan
author
2022-08-09
2297-1769
http://hdl.handle.net/10342/11806
10.3389/fvets.2022.932474
Caenorhabditis elegans
volatile organic compounds
cancer detection
Non-invasive Cancer Detection in Canine Urine Through Caenorhabditis Elegans Chemotaxis
oai:TheScholarship.intra.ecu.edu:10342/59772021-03-03T21:08:37Zcom_10342_107com_10342_73com_10342_74col_10342_5056col_10342_96
00925njm 22002777a 4500
dc
Guo, Lu
author
Wang, Gang
author
Feng, Yuanming
author
Yu, Tonggang
author
Guo, Yu
author
Bai, Xu
author
Ye, Zhaoxiang
author
2016-09-21
Abstract
Accurate target volume delineation is crucial for the radiotherapy of tumors. Diffusion and perfusion magnetic resonance imaging (MRI) can provide functional information about brain tumors, and they are able to detect tumor volume and physiological changes beyond the lesions shown on conventional MRI. This review examines recent studies that utilized diffusion and perfusion MRI for tumor volume definition in radiotherapy of brain tumors, and it presents the opportunities and challenges in the integration of multimodal functional MRI into clinical practice. The results indicate that specialized and robust post-processing algorithms and tools are needed for the precise alignment of targets on the images, and comprehensive validations with more clinical data are important for the improvement of the correlation between histopathologic results and MRI parameter images.
Radiation Oncology. 2016 Sep 21;11(1):123
http://dx.doi.org/10.1186/s13014-016-0702-y
http://hdl.handle.net/10342/5977
Brain tumors
Diffusion
Perfusion
Radiotherapy
Tumor volume definition
Diffusion and perfusion weighted magnetic resonance imaging for tumor volume definition in radiotherapy of brain tumors
oai:TheScholarship.intra.ecu.edu:10342/57262021-03-03T21:11:36Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Elhammali, Adnan
author
Patel, Mukund
author
Weinberg, Benjamin
author
Verma, Vivek
author
Liu, Jingxia
author
Olsen, Jeffrey R.
author
Gay, Hiram A.
author
2015-09
Background
To consolidate literature reports of serious late gastrointestinal toxicities after hypofractionated radiation treatment of pancreatic cancer and attempt to derive normal tissue complication probability (NTCP) parameters using the Lyman-Kutcher-Burman model.
Methods
Published reports of late grade 3 or greater gastrointestinal toxicity after hypofractionated treatment of pancreatic cancer were reviewed. The biologically equivalent dose in 1.8 Gy fractions was calculated using the EQD model. NTCP parameters were calculated using the LKB model assuming 1–5 % of the normal tissue volume was exposed to the prescription dose with α/β ratios of 3 or 4.
Results
A total of 16 human studies were examined encompassing a total of 1160 patients. Toxicities consisted of ulcers, hemorrhages, obstructions, strictures, and perforations. Non-hemorrhagic and non-perforated ulcers occurred at a rate of 9.1 % and were the most commonly reported toxicity. Derived NTCP parameter ranges were as follows: n = 0.38–0.63, m = 0.48–0.49, and TD50 = 35–95 Gy. Regression analysis showed that among various study characteristics, dose was the only significant predictor of toxicity.
Conclusions
Published gastrointestinal toxicity reports after hypofractionated radiotherapy for pancreatic cancer were compiled. Median dose was predictive of late grade ≥ 3 gastrointestinal toxicity. Preliminary NTCP parameters were derived for multiple volume constraints.
Radiation Oncology (London, England); 10:186 p. 1-8
1748-717X
http://hdl.handle.net/10342/5726
pmc4558934
10.1186/s13014-015-0489-2
Late gastrointestinal tissue effects after hypofractionated radiation therapy of the pancreas
oai:TheScholarship.intra.ecu.edu:10342/123572023-02-28T08:16:23Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Jo, Jasmin
author
et al
author
2022-02-08
1522-8517
http://hdl.handle.net/10342/12357
10.1093/neuonc/noac031
astrocytoma
IDH mutated glioma
oligodendroglioma
Surveillance Imaging Frequency in Adult Patients with Lower-Grade (WHO Grade 2 and 3) Gliomas
oai:TheScholarship.intra.ecu.edu:10342/117292022-11-10T08:16:26Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Muzaffar, Mahvish
author
Navaid, Musharraf
author
Naqash, Abdul Rafeh
author
2022
2072-6694
http://hdl.handle.net/10342/11729
10.3390/cancers14010186
hepatocellular carcinoma
inflammatory biomarkers
neutrophil-lymphocyte ratio
The Systemic Inflammatory Response Identifies Patients with Adverse Clinical Outcome from Immunotherapy in Hepatocellular Carcinoma
oai:TheScholarship.intra.ecu.edu:10342/57392021-03-03T21:11:09Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Dong, Lixue
author
Li, Zhigang
author
Leffler, Nancy R.
author
Asch, Adam S.
author
Chi, Jen-Tsan
author
Yang, Li V.
author
2013
Acidic tissue microenvironment commonly exists in inflammatory diseases, tumors, ischemic organs, sickle cell disease, and many other pathological conditions due to hypoxia, glycolytic cell metabolism and deficient blood perfusion. However, the molecular mechanisms by which cells sense and respond to the acidic microenvironment are not well understood. GPR4 is a proton-sensing receptor expressed in endothelial cells and other cell types. The receptor is fully activated by acidic extracellular pH but exhibits lesser activity at the physiological pH 7.4 and minimal activity at more alkaline pH. To delineate the function and signaling pathways of GPR4 activation by acidosis in endothelial cells, we compared the global gene expression of the acidosis response in primary human umbilical vein endothelial cells (HUVEC) with varying level of GPR4. The results demonstrated that acidosis activation of GPR4 in HUVEC substantially increased the expression of a number of inflammatory genes such as chemokines, cytokines, adhesion molecules, NF-κB pathway genes, and prostaglandin-endoperoxidase synthase 2 (PTGS2 or COX-2) and stress response genes such as ATF3 and DDIT3 (CHOP). Similar GPR4-mediated acidosis induction of the inflammatory genes was also noted in other types of endothelial cells including human lung microvascular endothelial cells and pulmonary artery endothelial cells. Further analyses indicated that the NF-κB pathway was important for the acidosis/GPR4-induced inflammatory gene expression. Moreover, acidosis activation of GPR4 increased the adhesion of HUVEC to U937 monocytic cells under a flow condition. Importantly, treatment with a recently identified GPR4 antagonist significantly reduced the acidosis/GPR4-mediated endothelial cell inflammatory response. Taken together, these results show that activation of GPR4 by acidosis stimulates the expression of a wide range of inflammatory genes in endothelial cells. Such inflammatory response can be suppressed by GPR4 small molecule inhibitors and hold potential therapeutic value.
PLoS ONE; 8:4 p. 1-15
1932-6203
http://hdl.handle.net/10342/5739
pmc3628782
10.1371/journal.pone.0061991
Acidosis Activation of the Proton-Sensing GPR4 Receptor Stimulates Vascular Endothelial Cell Inflammatory Responses Revealed by Transcriptome Analysis
oai:TheScholarship.intra.ecu.edu:10342/110522022-09-09T07:16:20Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Muzaffar, Mahvish
author
Navaid, Musharraf
author
Bulumulle, Anushi
author
Fessas, Petros
author
Naeem, Muntaha
author
Pinterb, Matthias
author
Marron, Thomas U.
author
Szafron, David
author
Balcar, Lorenz
author
Saeed, Anwaar
author
Jun, Tomi
author
Dharmapuri, Sirish
author
Gampa, Anuhya
author
Wang, Yinghong
author
Khan, Uqba
author
Lee, Pei-Chang
author
Yu, Bo
author
Paul, Sonal
author
Nimkar, Neil
author
Bettinger, Dominik
author
Hildebrand, Hannah
author
Abugabal, Yehia I.
author
Pressiani, Tiziana
author
Personeni, Nicola
author
Nishida, Naoshi
author
Kudo, Masatoshi
author
Kaseb, Ahmed
author
Huang, Yi-Hsiang
author
Ang, Celina
author
Pillai, Anjana
author
Rimassa, Lorenza
author
Naqash, Abdul Rafeh
author
Sharon, Elad
author
Cortellini, Alessio
author
Pinato, David J.
author
2021-10-08
1664-5553
http://hdl.handle.net/10342/11052
10.1159/000519108
Antibiotics
Cancer immunotherapy
Hepatocellular carcinoma
Early Antibiotic Exposure Is Not Detrimental to Therapeutic Effect from Immunotherapy in Hepatocellular Carcinoma
oai:TheScholarship.intra.ecu.edu:10342/117922022-12-01T08:18:52Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Woody, Susan
author
Hegde, Aparna
author
Arastu, Hyder
author
Peach, M. Sean
author
Ju, Andrew W.
author
Sharma, Nitika
author
Walker, Paul
author
2022-05-27
2234-943X
http://hdl.handle.net/10342/11792
10.3389/fonc.2022.785350
stereotactic body radiotherapy (SBRT)
stereotactic radiosurgery (SRS)
abscopal effect
Survival Is Worse in Patients Completing Immunotherapy Prior to SBRT/SRS Compared to Those Receiving It Concurrently or After
oai:TheScholarship.intra.ecu.edu:10342/121712023-02-04T08:16:20Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Muzaffar, Mahvish
author
Marinelli, Brett
author
2022
2051-1426
http://hdl.handle.net/10342/12171
10.1136/jitc-2021-004205
Transarterial chemoembolization
Nivolumab
hepatocellular carcinoma
Integrated Use of PD-1 Inhibition and Transarterial Chemoembolization for Hepatocellular Carcinoma: Evaluation of Safety and Efficacy in a Retrospective, Propensity Score-Matched Study
oai:TheScholarship.intra.ecu.edu:10342/123592023-02-28T08:16:24Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Jo, Jasmin
author
Donahue, Joseph
author
Sarai, Guneet
author
Petroni, Gina
author
Schiff, David
author
2022
1522-8517
http://hdl.handle.net/10342/12359
10.1093/neuonc/noab198
glioblastoma
intracranial hemorrhage
venous thromboembolism
Management of Venous Thromboembolism in High-Grade Glioma: Does Low Molecular Weight Heparin Increase Intracranial Bleeding Risk?
oai:TheScholarship.intra.ecu.edu:10342/57292021-03-03T21:11:03Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Kobet, Robert A.
author
Pan, Xiaoping
author
Zhang, Baohong
author
Pak, Stephen C.
author
Asch, Adam S.
author
Lee, Myon-Hee
author
2014-09
The nematode Caenorhabditis elegans (C. elegans) offers a unique opportunity for biological and basic medical researches due to its genetic tractability and well-defined developmental lineage. It also provides an exceptional model for genetic, molecular, and cellular analysis of human disease-related genes. Recently, C. elegans has been used as an ideal model for the identification and functional analysis of drugs (or small-molecules) in vivo. In this review, we describe conserved oncogenic signaling pathways (Wnt, Notch, and Ras) and their potential roles in the development of cancer stem cells. During C. elegans germline development, these signaling pathways regulate multiple cellular processes such as germline stem cell niche specification, germline stem cell maintenance, and germ cell fate specification. Therefore, the aberrant regulations of these signaling pathways can cause either loss of germline stem cells or overproliferation of a specific cell type, resulting in sterility. This sterility phenotype allows us to identify drugs that can modulate the oncogenic signaling pathways directly or indirectly through a high-throughput screening. Current in vivo or in vitro screening methods are largely focused on the specific core signaling components. However, this phenotype-based screening will identify drugs that possibly target upstream or downstream of core signaling pathways as well as exclude toxic effects. Although phenotype-based drug screening is ideal, the identification of drug targets is a major challenge. We here introduce a new technique, called Drug Affinity Responsive Target Stability (DARTS). This innovative method is able to identify the target of the identified drug. Importantly, signaling pathways and their regulators in C. elegans are highly conserved in most vertebrates, including humans. Therefore, C. elegans will provide a great opportunity to identify therapeutic drugs and their targets, as well as to understand mechanisms underlying the formation of cancer.
Biomolecules & Therapeutics; 22:5 p. 371-383
1976-9148
http://hdl.handle.net/10342/5729
pmc4201220
10.4062/biomolther.2014.084
Caenorhabditis elegans
Wnt
Notch
Ras
Cancer stem cells
Drug screening
: A Model System for Anti-Cancer Drug Discovery and Therapeutic Target Identification
oai:TheScholarship.intra.ecu.edu:10342/57332021-03-03T21:11:30Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Nguyen, Mai Lin
author
Willows, Brooke
author
Khan, Rihan
author
Chi, Alexander
author
Kim, Lyndon
author
Nour, Sherif G.
author
Sroka, Thomas
author
Kerr, Christine
author
Godinez, Juan
author
Mills, Melissa
author
Karlsson, Ulf
author
Altdorfer, Gabor
author
Nguyen, Nam P.
author
Jendrasiak, Gordon
author
2014-05
Magnetic resonance spectroscopy (MRS) is a non-invasive technique to detect metabolites within the normal and tumoral tissues. The ability of MRS to diagnose areas of high metabolic activity linked to tumor cell proliferation is particularly useful for radiotherapy treatment planning because of better gross tumor volume (GTV) delineation. The GTV may be targeted with higher radiation dose, potentially improving local control without excessive irradiation to the normal adjacent tissues. Prostate cancer and glioblastoma multiforme (GBM) are two tumor models that are associated with a heterogeneous tumor distribution. Preliminary studies suggest that the integration of MRS into radiotherapy planning for these tumors is feasible and safe. Image-guided radiotherapy (IGRT) by virtue of daily tumor imaging and steep dose gradient may allow for tumor dose escalation with the simultaneous integrated boost technique (SIB) and potentially decrease the complications rates in patients with GBM and prostate cancers.
Frontiers in Oncology; 4: p. 1-6
2234-943X
http://hdl.handle.net/10342/5733
pmc4017160
10.3389/fonc.2014.00091
MRS
IGRT
prostate cancer
GBM
The Potential Role of Magnetic Resonance Spectroscopy in Image-Guided Radiotherapy
oai:TheScholarship.intra.ecu.edu:10342/57382021-03-03T21:11:05Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Li, Zhigang
author
Dong, Lixue
author
Dean, Eric
author
Yang, Li V.
author
2013-10
Acidosis is a biochemical hallmark of the tumor microenvironment. Here, we report that acute acidosis decreases c-Myc oncogene expression in U937 human lymphoma cells. The level of c-Myc transcripts, but not mRNA or protein stability, contributes to c-Myc protein reduction under acidosis. The pH-sensing receptor TDAG8 (GPR65) is involved in acidosis-induced c-Myc downregulation. TDAG8 is expressed in U937 lymphoma cells, and the overexpression or knockdown of TDAG8 further decreases or partially rescues c-Myc expression, respectively. Acidic pH alone is insufficient to reduce c-Myc expression, as it does not decrease c-Myc in H1299 lung cancer cells expressing very low levels of pH-sensing G protein-coupled receptors (GPCRs). Instead, c-Myc is slightly increased by acidosis in H1299 cells, but this increase is completely inhibited by ectopic overexpression of TDAG8. Interestingly, TDAG8 expression is decreased by more than 50% in human lymphoma samples in comparison to non-tumorous lymph nodes and spleens, suggesting a potential tumor suppressor function of TDAG8 in lymphoma. Collectively, our results identify a novel mechanism of c-Myc regulation by acidosis in the tumor microenvironment and indicate that modulation of TDAG8 and related pH-sensing receptor pathways may be exploited as a new approach to inhibit Myc expression.
International Journal of Molecular Sciences; 14:10 p. 20236-20255
1422-0067
http://hdl.handle.net/10342/5738
pmc3821613
10.3390/ijms141020236
acidosis
tumor microenvironment
TDAG8
GPR65
c-Myc
Acidosis Decreases c-Myc Oncogene Expression in Human Lymphoma Cells: A Role for the Proton-Sensing G Protein-Coupled Receptor TDAG8
oai:TheScholarship.intra.ecu.edu:10342/57372021-03-03T21:11:02Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Fitzgerald, Timothy L.
author
Zervos, Emmanuel
author
Wong, Jan H.
author
2013
High-level evidence supports adjuvant radiotherapy for rectal cancer. We examined the influence of sociodemographic factors on patterns of adjuvant radiotherapy for resected Stage II/III rectal cancer. Methods. Patients undergoing surgical resection for stage II/III rectal cancer were identified in SEER registry. Results. A total of 21,683 patients were identified. Majority of patients were male (58.8%), white (83%), and with stage III (54.9%) and received radiotherapy (66%). On univariate analysis, male gender, stage III, younger age, year of diagnosis, and higher socioeconomic status (SES) were associated with radiotherapy. Radiotherapy was delivered in 84.4% of patients 50; however, only 32.8% of those are 80 years. Logistic regression demonstrated a significant increase in the use of radiotherapy in younger patients who are 50 (OR, 10.3), with stage III (OR, 1.21), males (OR, 1.18), and with higher SES. Conclusions. There is a failure to conform to standard adjuvant radiotherapy in one-third of patients, and this is associated with older age, stage II, area-level of socioeconomic deprivation, and female sex.
Journal of Cancer Epidemiology; 2013: p. 1-6
1687-8558
http://hdl.handle.net/10342/5737
pmc3808718
10.1155/2013/408460
Patterns of Pelvic Radiotherapy in Patients with Stage II/III Rectal Cancer
oai:TheScholarship.intra.ecu.edu:10342/57362022-12-09T15:32:14Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Joh, Daniel Y.
author
Chen, Leonard N.
author
Porter, Gerald
author
Bhagat, Aditi
author
Sood, Sumit
author
Kim, Joy S.
author
Moures, Rudy
author
Yung, Thomas
author
Lei, Siyuan
author
Collins, Brian T.
author
Ju, Andrew Wenhua
author
Suy, Simeng
author
Carroll, John M.
author
Lynch, John H.
author
Dritschilo, Anatoly
author
Collins, Sean P.
author
2014-12
Background
Proctitis after radiation therapy for prostate cancer remains an ongoing clinical challenge and critical quality of life issue. SBRT could minimize rectal toxicity by reducing the volume of rectum receiving high radiation doses and offers the potential radiobiologic benefits of hypofractionation. This study sought to evaluate the incidence and severity of proctitis following SBRT for prostate cancer.
Methods
Between February 2008 and July 2011, 269 men with clinically localized prostate cancer were treated definitively with SBRT monotherapy at Georgetown University Hospital. All patients were treated to 35-36.25Gy in 5 fractions delivered with the CyberKnife Radiosurgical System (Accuray). Rectal bleeding was recorded and scored using the CTCAE v.4. Telangiectasias were graded using the Vienna Rectoscopy Score (VRS). Proctitis was assessed via the Bowel domain of the Expanded Prostate Index Composite (EPIC)-26 at baseline and at 1, 3, 6, 9, 12, 18 and 24 months post-SBRT.
Results
The median age was 69 years with a median prostate volume of 39 cc. The median follow-up was 3.9 years with a minimum follow-up of two years. The 2-year actuarial incidence of late rectal bleeding ≥ grade 2 was 1.5%. Endoscopy revealed VRS Grade 2 rectal telangiectasias in 11% of patients. All proctitis symptoms increased at one month post-SBRT but returned to near-baseline with longer follow-up. The most bothersome symptoms were bowel urgency and frequency. At one month post-SBRT, 11.2% and 8.5% of patients reported a moderate to big problem with bowel urgency and frequency, respectively. The EPIC bowel summary scores declined transiently at 1 month and experienced a second, more protracted decline between 6 months and 18 months before returning to near-baseline at two years post-SBRT. Prior to treatment, 4.1% of men felt their bowel function was a moderate to big problem which increased to 11.5% one month post-SBRT but returned to near-baseline at two years post-SBRT.
Conclusions
In this single institution cohort, the rate and severity of proctitis observed following SBRT is low. QOL decreased on follow-up; however, our results compare favorably to those reported for patients treated with alternative radiation modalities. Future prospective randomized studies are needed to confirm these observations.
Radiation Oncology (London, England); 9: p. 1-10
1748-717X
http://hdl.handle.net/10342/5736
pmc4272823
10.1186/s13014-014-0277-4
Prostate cancer
SBRT
Rectal endoscopy
Telangiectasias
CyberKnife
Expanded prostate index composite
Bother
Proctitis
Rectal bleeding
Vienna rectoscopy score
Proctitis following stereotactic body radiation therapy for prostate cancer
oai:TheScholarship.intra.ecu.edu:10342/75472022-12-09T19:12:43Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Lu, Qun
author
Chen, Yan-Hua
author
2008-11-04
The present invention provides a method for detecting or screening for the presence of cancer in a subject. The method comprises obtaining, providing or collecting a tissue or fluid sample (such as a urine sample) from said subject, and then determining the presence or absence of delta-catenin in said sample, or increased levels of delta-catenin in said sample as compared to a normal or control subject. The presence of delta-catentin in said sample, or increased levels of delta-catenin in said sample, indicating said subject is afflicted with or at least at risk of developing cancer.
US Pat# US7445906B2
http://hdl.handle.net/10342/7547
cancer
screening
delta-catenin
Method of detecting cancer using delta-catenin
oai:TheScholarship.intra.ecu.edu:10342/57282021-03-03T21:10:55Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Wang, Ping
author
Feng, Yuanming
author
2013
Purpose. To develop a clinically viable mathematical model that quantitatively predicts tumor volume change during radiotherapy in order to provide treatment response assessment for prognosis, treatment plan optimization, and adaptation. Method and Materials. The correction factors containing hypoxia, DNA single strand breaks, potentially lethal damage, and other factors were used to develop an improved cell survival model based on the popular linear-quadratic model of cell survival in radiotherapy. The four-level cell population model proposed by Chvetsov et al. was further simplified by removing the initial hypoxic fraction and reoxygenation parameter, which are hard to obtain in routine clinics, such that an easy-to-use model can be developed for clinical applications. The new model was validated with data of nine lung and cervical cancer patients. Results. Out of the nine cases, the new model can predict tumor volume change in six cases with a correlation index R2 greater than 0.9 and the rest of three with R2 greater than 0.85. Conclusion. Based on a four-level cell population model, a more practical and simplified cell survival curve was proposed to model the tumor volume changes during radiotherapy. Validation study with patient data demonstrated feasibility and clinical usefulness of the new model in predicting tumor volume change in radiotherapy.
The Scientific World Journal; 2013: p. 1-5
1537-744X
http://hdl.handle.net/10342/5728
pmc3814055
10.1155/2013/181070
A Mathematical Model of Tumor Volume Changes during Radiotherapy
oai:TheScholarship.intra.ecu.edu:10342/109602022-08-02T07:16:06Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Ali, Hassam
author
Pamarthy, Rahul
author
Vallabhaneni, Meghana
author
Sarfraz, Shiza
author
Ali, Hadiqa
author
Rafique, Hamza
author
2021
2046-1402
http://hdl.handle.net/10342/10960
10.12688/f1000research.54390.1
Pancreatic cancer
SEER database
PNETs
Pancreatic Cancer Incidence Trends in the United States from 2000-2017: Analysis of Surveillance, Epidemiology and End Results (SEER) Database
oai:TheScholarship.intra.ecu.edu:10342/116712022-11-02T07:16:17Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Khanchandani, Ashish T.
author
Larkins, Michael C.
author
Tooley, Ann M.
author
Meyer, David B.
author
Chaudhary, Vijay
author
Fallon, John T.
author
2022
2374-2895
http://hdl.handle.net/10342/11671
10.1016/j.acpath.2022.100038
Pathology report
cancer
Health literacy
The Impact of Curated Educational Videos on Pathology Health Literacy for Patients with a Pancreatic, Colorectal, or Prostate Cancer Diagnosis
oai:TheScholarship.intra.ecu.edu:10342/57322021-03-03T21:11:24Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Justus, Calvin R.
author
Dong, Lixue
author
Yang, Li V.
author
2013-10
The tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion. It is proposed that acidosis in the tumor microenvironment is an important stress factor and selection force for cancer cell somatic evolution. Acidic pH has pleiotropic effects on the proliferation, migration, invasion, metastasis, and therapeutic response of cancer cells and the function of immune cells, vascular cells, and other stromal cells. However, the molecular mechanisms by which cancer cells and stromal cells sense and respond to acidic pH in the tumor microenvironment are poorly understood. In this article the role of a family of pH-sensing G protein-coupled receptors (GPCRs) in tumor biology is reviewed. Recent studies show that the pH-sensing GPCRs, including GPR4, GPR65 (TDAG8), GPR68 (OGR1), and GPR132 (G2A), regulate cancer cell metastasis and proliferation, immune cell function, inflammation, and blood vessel formation. Activation of the proton-sensing GPCRs by acidosis transduces multiple downstream G protein signaling pathways. Since GPCRs are major drug targets, small molecule modulators of the pH-sensing GPCRs are being actively developed and evaluated. Research on the pH-sensing GPCRs will continue to provide important insights into the molecular interaction between tumor and its acidic microenvironment and may identify new targets for cancer therapy and chemoprevention.
Frontiers in Physiology; 4: p. 1-9
1664-042X
http://hdl.handle.net/10342/5732
pmc3851830
10.3389/fphys.2013.00354
cancer
tumor microenvironment
acidosis
proton-sensing G protein-coupled receptors
GPR4
GPR65
(TDAG8)
GPR68 (OGR1)
GPR132 (G2A)
Acidic tumor microenvironment and pH-sensing G protein-coupled receptors
oai:TheScholarship.intra.ecu.edu:10342/57412021-03-03T21:11:19Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Li, Taoran
author
Zhu, Xiaofeng
author
Thongphiew, Danthai
author
Lee, W. Robert
author
Vujaskovic, Zeljko
author
Wu, Qiuwen
author
Yin, Fang-Fang
author
Wu, Q. Jackie
author
2010
The purpose of this paper is to evaluate the feasibility and clinical dosimetric benefit of an on-line, that is, with the patient in the treatment position, Adaptive Radiation Therapy (ART) system for prostate cancer treatment based on daily cone-beam CT imaging and fast volumetric reoptimization of treatment plans. A fast intensity-modulated radiotherapy (IMRT) plan reoptimization algorithm is implemented and evaluated with clinical cases. The quality of these adapted plans is compared to the corresponding new plans generated by an experienced planner using a commercial treatment planning system and also evaluated by an in-house developed tool estimating achievable dose-volume histograms (DVHs) based on a database of existing treatment plans. In addition, a clinical implementation scheme for ART is designed and evaluated using clinical cases for its dosimetric qualities and efficiency.
Journal of Oncology; 2010: p. 1-12
1687-8450
http://hdl.handle.net/10342/5741
pmc2990023
10.1155/2010/407236
On-Line Adaptive Radiation Therapy: Feasibility and Clinical Study
oai:TheScholarship.intra.ecu.edu:10342/123452023-02-21T08:16:15Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Amar, Suneetha
author
Muzaffar, Mahvish
author
Naqash, Abdul Rafeh
author
Vithayathil, Mathew
author
et al
author
2022
1478-3223
http://hdl.handle.net/10342/12345
10.1111/liv.15405
anti-programmed death-ligand
anti-vascular endothelial growth factor
immunotherapy
Impact of Older Age in Patients Receiving Atezolizumab and Bevacizumab for Hepatocellular Carcinoma
oai:TheScholarship.intra.ecu.edu:10342/121822023-02-07T08:16:38Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Corns, Robert
author
Yang, Kaida
author
Ross, Mason
author
Bhandari, Shiva
author
Aryal, Makunda
author
Ciaccio, Peter
author
2022
1526-9914
http://hdl.handle.net/10342/12182
10.1002/acm2.13623
3D star shot
linear accelerator
axes positions
A 3D Star Shot to Determine the Gantry, Collimator, and Couch Axes Positions
oai:TheScholarship.intra.ecu.edu:10342/51962021-03-03T20:58:59Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Jenkins, Todd
author
Allison, Ron R.
author
Sibata, Claudio
author
2008-05-25
A three-dimensional phantom assembly for use with a radiation treatment device includes a three-dimensional support member having at least two, spaced apart opposed surfaces configured to hold at least one generally planar radiation sensitive dosimeter sheet such that the dosimeter sheet generally conforms to a shape defined by the two opposed surfaces. During irradiation, a radiation beam trajectory passes through the two opposed surfaces. Related systems methods for determining a radiation isocenter and/or generating a 3-D visualization of the radiation isocenter using radiation patterns obtained using a phantom are also described.
U.S. Patent No. 7,349,523
http://hdl.handle.net/10342/5196
Radiation isocenter measurement devices and methods and 3-D radiation isocenter visualization systems and related methods
oai:TheScholarship.intra.ecu.edu:10342/51662021-03-03T20:58:22Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Anagnostou, Athanasius A.
author
Sigounas, George
author
1999-07-13
The use of human erythropoietin (EPO) to prevent or treat endothelial injury due to chemotherapy, radiation therapy, mechanical trauma, or to a disease state which damages the endothelium (such as inflammation, heart disease or cancer) is described. The use of EPO in conjunction with the administration of chemotherapeutic agents is described.
U.S. Patent No. 5,922,674
http://hdl.handle.net/10342/5166
Method of treatment using chemotherapy and erythropoietin
oai:TheScholarship.intra.ecu.edu:10342/57312021-03-03T21:11:21Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Cavalieri, Ronaldo
author
Gay, Hiram A.
author
Liu, Jingxia
author
Ferreira, Maria Clara
author
Mota, Helvecio C.
author
Sibata, Claudio H.
author
Allison, Ron R.
author
2011
Background
To evaluate the daily total error shift patterns on post-prostatectomy patients undergoing image guided radiotherapy (IGRT) with a diagnostic quality computer tomography (CT) on rails system.
Methods
A total of 17 consecutive post-prostatectomy patients receiving adjuvant or salvage IMRT using CT-on-rails IGRT were analyzed. The prostate bed's daily total error shifts were evaluated for a total of 661 CT scans.
Results
In the right-left, cranial-caudal, and posterior-anterior directions, 11.5%, 9.2%, and 6.5% of the 661 scans required no position adjustments; 75.3%, 66.1%, and 56.8% required a shift of 1 - 5 mm; 11.5%, 20.9%, and 31.2% required a shift of 6 - 10 mm; and 1.7%, 3.8%, and 5.5% required a shift of more than 10 mm, respectively. There was evidence of correlation between the x and y, x and z, and y and z axes in 3, 3, and 3 of 17 patients, respectively. Univariate (ANOVA) analysis showed that the total error pattern was random in the x, y, and z axis for 10, 5, and 2 of 17 patients, respectively, and systematic for the rest. Multivariate (MANOVA) analysis showed that the (x,y), (x,z), (y,z), and (x, y, z) total error pattern was random in 5, 1, 1, and 1 of 17 patients, respectively, and systematic for the rest.
Conclusions
The overall daily total error shift pattern for these 17 patients simulated with an empty bladder, and treated with CT on rails IGRT was predominantly systematic. Despite this, the temporal vector trends showed complex behaviors and unpredictable changes in magnitude and direction. These findings highlight the importance of using daily IGRT in post-prostatectomy patients.
Radiation Oncology (London, England); 6: p. 142-142
1748-717X
http://hdl.handle.net/10342/5731
pmc3220642
10.1186/1748-717X-6-142
Post-prostatectomy radiotherapy
IGRT
CT on rails
shift total error
prostate bed
Total error shift patterns for daily CT on rails image-guided radiotherapy to the prostate bed
oai:TheScholarship.intra.ecu.edu:10342/57252021-03-03T21:11:32Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Gay, Hiram A.
author
Taylor, Quendella Q.
author
Kiriyama, Fumika
author
Dieck, Geoffrey T.
author
Jenkins, Todd
author
Walker, Paul
author
Allison, Ron R.
author
Ubezio, Paolo
author
2013
Background. To characterize the lung tumor volume response during conventional and hypofractionated radiotherapy (RT) based on diagnostic quality CT images prior to each treatment fraction. Methods. Out of 26 consecutive patients who had received CT-on-rails IGRT to the lung from 2004 to 2008, 18 were selected because they had lung lesions that could be easily distinguished. The time course of the tumor volume for each patient was individually analyzed using a computer program. Results. The model fits of group L (conventional fractionation) patients were very close to experimental data, with a median Δ% (average percent difference between data and fit) of 5.1% (range 3.5–10.2%). The fits obtained in group S (hypofractionation) patients were generally good, with a median Δ% of 7.2% (range 3.7–23.9%) for the best fitting model. Four types of tumor responses were observed—Type A: “high� kill and “slow� dying rate; Type B: “high� kill and “fast� dying rate; Type C: “low� kill and “slow� dying rate; and Type D: “low� kill and “fast� dying rate. Conclusions. The models used in this study performed well in fitting the available dataset. The models provided useful insights into the possible underlying mechanisms responsible for the RT tumor volume response.
Computational and Mathematical Methods in Medicine; 2013: p. 1-13
1748-670X
http://hdl.handle.net/10342/5725
pmc3821906
10.1155/2013/637181
Modeling of Non-Small Cell Lung Cancer Volume Changes during CT-Based Image Guided Radiotherapy: Patterns Observed and Clinical Implications
oai:TheScholarship.intra.ecu.edu:10342/57352021-03-03T21:11:40Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Guo, Lu
author
Shen, Shuming
author
Harris, Eleanor
author
Wang, Zheng
author
Jiang, Wei
author
Guo, Yu
author
Feng, Yuanming
author
2014
Purpose
To develop a tri-modality image fusion method for better target delineation in image-guided radiotherapy for patients with brain tumors.
Methods
A new method of tri-modality image fusion was developed, which can fuse and display all image sets in one panel and one operation. And a feasibility study in gross tumor volume (GTV) delineation using data from three patients with brain tumors was conducted, which included images of simulation CT, MRI, and 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET) examinations before radiotherapy. Tri-modality image fusion was implemented after image registrations of CT+PET and CT+MRI, and the transparency weight of each modality could be adjusted and set by users. Three radiation oncologists delineated GTVs for all patients using dual-modality (MRI/CT) and tri-modality (MRI/CT/PET) image fusion respectively. Inter-observer variation was assessed by the coefficient of variation (COV), the average distance between surface and centroid (ADSC), and the local standard deviation (SDlocal). Analysis of COV was also performed to evaluate intra-observer volume variation.
Results
The inter-observer variation analysis showed that, the mean COV was 0.14(±0.09) and 0.07(±0.01) for dual-modality and tri-modality respectively; the standard deviation of ADSC was significantly reduced (p<0.05) with tri-modality; SDlocal averaged over median GTV surface was reduced in patient 2 (from 0.57 cm to 0.39 cm) and patient 3 (from 0.42 cm to 0.36 cm) with the new method. The intra-observer volume variation was also significantly reduced (p = 0.00) with the tri-modality method as compared with using the dual-modality method.
Conclusion
With the new tri-modality image fusion method smaller inter- and intra-observer variation in GTV definition for the brain tumors can be achieved, which improves the consistency and accuracy for target delineation in individualized radiotherapy.
PLoS ONE; 9:11 p. 1-9
1932-6203
http://hdl.handle.net/10342/5735
pmc4223022
10.1371/journal.pone.0112187
A Tri-Modality Image Fusion Method for Target Delineation of Brain Tumors in Radiotherapy
oai:TheScholarship.intra.ecu.edu:10342/122122023-02-09T08:16:16Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Snyder, Rebecca A.
author
2022-03-17
0732-183X
http://hdl.handle.net/10342/12212
10.1200/JCO.22.00068
cancer
treatment
Losing patients
A Soft Spot
oai:TheScholarship.intra.ecu.edu:10342/30382021-03-03T20:54:51Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Lee, Tung-Kwang
author
Wang, Weidong
author
O'Brien, Kevin F.
author
Roberta, Johnke
author
Allison, Ron R.
author
Diaz, Angelica
author
Wang, Tao
author
2008-12
To explore the radioprotective effect of a standardized North American ginseng extract (NAGE) on human peripheral blood lymphocytes (PBL), a micronuclei (MN) assay was conducted in PBL obtained from 12 volunteers. NAGE (50â 1000 µg/mL) and WR-1065 (1 mm and 3 mm) were applied to PBL cultures at 0 h and 90 min post-irradiation. It was found that (1) the baseline MN yield of PBL ranged from 14.4 ± 1.5 to 15.9 ± 1.5 per 1000 binucleated cells (p > 0.05); after irradiation (1 Gy and 2 Gy), the MN yield increased sharply; (2) MN yields declined with increasing concentrations of NAGE and WR-1065. Even at 90 min post-irradiation of 1 Gy, the maximum level of MN reduction rate caused by NAGE and WR-1065 was 53.8% and 59.2%, respectively; after 2 Gy irradiation, it was 37.3% and 42%, respectively; (3) the MN distribution in PBL followed a non-Poisson distribution in all cases; and (4) both NAGE and WR-1065 showed no significant effect on the proliferation index of lymphocytes. The results indicate that NAGE is a relatively non-toxic natural product, which can be administered as a dietary supplement and has the potential to be a radiation countermeasure. Originally published Phytotherapy Research Vol. 22, No. 12 2008.
Phytotherapy Research; 22:12 p. 1614-1622
http://hdl.handle.net/10342/3038
PMC2642735
10.1002/ptr.2533
Ginseng
WR-1065
Radioprotection
Micronuclei
Lymphocytes
Effect of North American Ginseng on 137Cs-induced Micronuclei in Human Lymphocytes
oai:TheScholarship.intra.ecu.edu:10342/115102022-10-14T07:15:57Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Bulumulle, Anushi
author
Brody, Heather
author
Macherla, Shravanti
author
Walker, Paul R.
author
Sehgal, Kartik
author
Gill, Ritu R.
author
Qilleri, Aleksandra
author
McDonald, Danielle C.
author
Cherry, Cynthia R.
author
Shea, Meghan
author
Huberman, Mark S.
author
VanderLaan, Paul A.
author
Weiss, Glen J.
author
Costa, Daniel B.
author
Rangachari, Deepa
author
2021
1525-7304
http://hdl.handle.net/10342/11510
10.1016/j.cllc.2020.05.028
Dosing Intervals
Survival Outcomes
Nonesmall-cell Lung Cancer
Association of Extended Dosing Intervals or Delays in Pembrolizumab-Based Regimens With Survival Outcomes in Advanced Non-small-cell Lung Cancer
oai:TheScholarship.intra.ecu.edu:10342/118182022-12-06T08:16:51Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Muzaffar, Mahvish
author
Bulumulle, Anushi
author
Sharma, Rohini
author
2022
2471-254X
http://hdl.handle.net/10342/11818
10.1002/hep4.1927
immune checkpoint inhibitors (ICIs)
hepatocellular cancer (HCC)
tyrosine kinase inhibitors (TKIs)
Patterns and Outcomes of Subsequent Therapy After Immune Checkpoint Inhibitor Discontinuation in HCC
oai:TheScholarship.intra.ecu.edu:10342/57302021-03-03T21:11:07Zcom_10342_74com_10342_73col_10342_96
00925njm 22002777a 4500
dc
Liu, Yahui
author
Lin, Wang
author
Yang, Xu
author
Liang, Weizi
author
Zhang, Jun
author
Meng, Maobin
author
Rice, John R.
author
Sa, Yu
author
Feng, Yuanming
author
2014-01
Morphological identification is a widespread procedure to assess the presence of apoptosis by visual inspection of the morphological characteristics or the fluorescence images. The procedure is lengthy and results are observer dependent. A quantitative automatic analysis is objective and would greatly help the routine work. We developed an image processing and segmentation method which combined the Otsu thresholding and morphological operators for apoptosis study. An automatic determination method of apoptotic stages of HL-60 cells with fluorescence images was developed. Comparison was made between normal cells, early apoptotic cells and late apoptotic cells about their geometric parameters which were defined to describe the features of cell morphology. The results demonstrated that the parameters we chose are very representative of the morphological characteristics of apoptotic cells. Significant differences exist between the cells in different stages, and automatic quantification of the differences can be achieved.
EXCLI Journal; 13: p. 19-27
1611-2156
http://hdl.handle.net/10342/5730
pmc4464453
cell morphology
quantitative analysi
apoptosis
Automatic quantitative analysis of morphology of apoptotic HL-60 cells