2024-03-28T18:28:23Zhttps://thescholarship.ecu.edu/oai/requestoai:TheScholarship.intra.ecu.edu:10342/45632021-03-03T20:57:57Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats
Holland, Nathan A.
Wingard, Christopher J.
The uses of engineered nanomaterials have expanded in biomedical technology and consumer manufacturing. Exposure to particulate matter has been demonstrated to negatively influence cardiovascular health and expand myocardial infarction. Furthermore, pulmonary exposure to various engineered nanomaterials has, likewise, demonstrated the ability to exacerbate cardiac ischemia reperfusion (I/R) injury. We hypothesized that pulmonary exposure to AgNP induces cardiovascular toxicity in the form of expanded I/R injury, electrical dysfunction and inducing a persistent increase in circulating proinflammatory cytokines. To test this hypothesis, we exposed male SD rats to an intratracheal (IT) instillation of 200 µg of 20 or 110 nm polyvinylprryolidone (PVP) or citrate capped AgNP, in 200 ul of the respective PVP or citrate vehicle. Serum samples were collected prior to instillation and 1, 3, 6, 24, 48, 72, and 168 hours following instillation. Serum samples were analyzed by multiplex assay for concentrations of: G-CSF, GM-CSF, MIP-1a, IL -1b, IL-2, IL-5, IL-6, IL-10, IL-13, IL-17a, IL-18, MCP-1, IFNy, RANTES, and TNFa. Twenty four and 168 hours after IT exposure, cardiac ischemia was induced by left anterior descending coronary artery ligation for 20 minutes followed by 2 hours of reperfusion. Intraoperative ECG was monitored throughout cardiac I/R surgery for heart rate (HR), PR interval, and QT interval. To test the impact of silver ion exposure on cardiac I/R injury we administered 200 ul of 0.01 mg/mL, 0.1 mg/mL, or 1 mg/mL silver acetate (AgAc) and induced cardiac I/R 24 hours later. Intratracheal instillation of AgNP resulted in expansion of I/R injury for both sizes of citrate and PVP capped AgNP at both 24 hours and 168 following instillation; exposure to 0.1 and 1 mg/mL AgAc also resulted in expansion of I/R injury. Intratracheal instillation of AgNP did not result in increased serum concentrations of selected proinflammatory cytokines, however post I/R serum levels of IL-2, IL-6, and IL-18 were significantly elevated in rats exposed to 20 nm PVP capped AgNP compared to vehicle controls at 24 hours post instillation. Instillation of AgNP had no impact on HR or QT interval. However, exposure to 20 nm AgNP resulted in a differential prolongation or shortening of PR interval during reperfusion based on capping agent. In conclusion IT instillation of AgNP exacerbates cardiac I/R injury 24 and 168 hours following instillation, without inducing a strong systemic inflammatory response or electrical dysfunction. Exposure to AgNP may result in a sensitization of the immune system in response to a secondary insult (e.g., cardiac I/R) which are largely correlated with capping agents and particle size and may drive expansion of I/R injury at 24 and 168 hours following IT instillation of AgNP. Â
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4563
https://thescholarship.ecu.edu/bitstream/10342/4563/1/Holland_ecu_0600O_11215.pdf
291c44e02f16e1c97ded2e2d5ae741d6
https://thescholarship.ecu.edu/bitstream/10342/4563/2/Holland_ecu_0600O_11215.pdf.txt
459d5150cb02fddcfa55a3e9a62dc30c
https://thescholarship.ecu.edu/bitstream/10342/4563/3/NEDL_Holland.pdf
894fcb5eefe752fd531ee4ea8ff806f4
Physiology
Toxicology
Cardiac
Inflammation
Ischemia
Nanomaterial
Reperfusion
Silver
Biology, Physiology
Myocardial Infarction
Reperfusion Injury
Nanoparticles--adverse effects
oai:TheScholarship.intra.ecu.edu:10342/104322022-03-29T07:18:43Zcom_10342_73com_10342_122col_10342_117col_10342_124
Investigation of pica history and trace elements in pica substances ingested by pregnant females in eastern North Carolina
Corbett, Robin Webb
Rose, Mary Ann
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10432
https://thescholarship.ecu.edu/bitstream/10342/10432/1/Corbett_Investigation-of-pica-history-and-trace_1985.pdf
a3b3b3898e9565127b0f2a1321fdadf9
https://thescholarship.ecu.edu/bitstream/10342/10432/2/Corbett_Investigation-of-pica-history-and-trace_1985.pdf.txt
e6e4a170207611764dda0a6930389a38
NURS
oai:TheScholarship.intra.ecu.edu:10342/38942021-03-03T20:57:47Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
The effectiveness of Farabloc technology with Mirror Therapy in reducing phantom limb pain in individuals with a unilateral lower extremity vascular amputation
Houston, Helen
Dickerson, Anne Elizabeth
Objective :the objective of this study was to investigate the effectiveness of combining two interventions, Farabloc technology to eliminate electromagnetic fields and Mirror Therapy to assist in the sensory cortex reorganization, to decrease or eliminate phantom limb pain in vascular amputees. Methods:fourteen older adults with a unilateral vascular amputation participated in the study. Nine individuals started the intervention within 48 hours of surgery and were compared to five individuals who were approximately 18 months post-surgery. Measures of residual limb edema and temperature, phantom limb pain variables, activities of daily living and quality of life interference were completed pre and post intervention and 4 weeks after the end of therapy. Results:all fourteen subjects reported an overall decrease in phantom limb pain using a visual analogue scale. For the acute group, wound healing and edema reduction decreased time to prosthetic fitting from 12 weeks to eight weeks, significant for improving functional ambulation, return to work and decreasing wheelchair mobility dependence. Activities of daily living and quality of life variables both showed significant differences. Conclusion:use of this combined treatment protocol shows promising results for not only acute amputee intervention, but also improved perception of pain and improved quality of life for amputees with chronic phantom limb pain. Implications for activities of daily living and quality of life are discussed
East Carolina University
2012
Master's Thesis
http://hdl.handle.net/10342/3894
https://thescholarship.ecu.edu/bitstream/10342/3894/5/Houston_ecu_0600M_10629.pdf.txt
25a6491bfe08b343f9dab7bc93a995f2
https://thescholarship.ecu.edu/bitstream/10342/3894/6/Houston%2520document%5b1%5d.pdf.txt
cb492b7df9b5c170d7c87527940eff3b
https://thescholarship.ecu.edu/bitstream/10342/3894/1/Houston_ecu_0600M_10629.pdf
aa3ef212aaa62dae655686f635a1792b
https://thescholarship.ecu.edu/bitstream/10342/3894/2/FinalTables.docx
79bf98b8f156bd3a218d64b7a06c429f
https://thescholarship.ecu.edu/bitstream/10342/3894/3/Houston%2520document%5b1%5d.pdf
12c16718577c675a9d4120a550b15967
https://thescholarship.ecu.edu/bitstream/10342/3894/4/Houston_2_of_2001.jpg
d35a6e396e7e2168b5ac4bd1c26e59e5
https://thescholarship.ecu.edu/bitstream/10342/3894/8/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/3894/9/Houston.pdf
12c16718577c675a9d4120a550b15967
Electromagnetics
Health sciences
Activities of Daily Living
Electromagnetic fields
Mirror therapy
Phantom limb pain
Quality of life
Vascular amputation
Phantom Limb--therapy
Electromagnetic Fields--therapeutic use
Somatosensory Cortex
Amputees
Pain Management--therapy
oai:TheScholarship.intra.ecu.edu:10342/48062021-03-03T20:56:49Zcom_10342_73com_10342_1col_10342_117col_10342_72
Preferred Distribution Methods of Health Information for Hispanics
Simmons, Cathryn
Larson, Kim L.
Emerging Hispanic communities in the U.S. have experienced barriers in health care access and health literacy. A program evaluation was conducted at a health department in eastern North Carolina to assess the preferred method of receiving health information by a sub-set of Hispanic adults. Evaluation data included an assessment tool completed by Hispanic adults receiving care at the local health department (LHD), key informant interviews, and 7 weeks of participant-observation at the LHD. The top three preferences for ages 18-35 were nurses (76%), doctors (76%), and family/friends/neighbors (70%). The top three preferences for ages 35 and older were family/friends/neighbors (65%), doctors (57%), and television (57%). The three preferences for the whole sample were doctors (68%), family/friends/neighbors (68%), and nurses (65%). The findings indicate a difference of preferences between the younger and older populations. Based on the results, a possible method to consider for distributing health information for the older adult Hispanic population may be to implement a CHW program, whereas methods to consider for distributing health information to the younger adult population may be through television, Internet, or radio.
2015
Honors Thesis
http://hdl.handle.net/10342/4806
https://thescholarship.ecu.edu/bitstream/10342/4806/1/Simmons_Honors_Final%20Paper.docx
01602d60dc15ff9eeaa1c5d183d3ffdc
https://thescholarship.ecu.edu/bitstream/10342/4806/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Hispanics
Latinos
Health information preferences
Information distribution
oai:TheScholarship.intra.ecu.edu:10342/104742022-03-29T07:21:32Zcom_10342_73com_10342_122col_10342_117col_10342_124
Comparison of values held about purposes in life and death anxiety attitudes
Hunter, Gwendolyn C.
Koldjeski, Helen D.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10474
https://thescholarship.ecu.edu/bitstream/10342/10474/1/Hunter_Comparison-of-values-held-about-purposes_1982.pdf
59111a3f4248a78d3fda990e5e415349
https://thescholarship.ecu.edu/bitstream/10342/10474/2/Hunter_Comparison-of-values-held-about-purposes_1982.pdf.txt
0ee4116f278f879b6d1570cd7ec11433
NURS
oai:TheScholarship.intra.ecu.edu:10342/103412023-01-04T16:37:50Zcom_10342_73com_10342_122col_10342_117col_10342_124
Post-placement needs of adoptive mothers
Cauley, Lisa W.
Poston, Iona
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10341
https://thescholarship.ecu.edu/bitstream/10342/10341/1/Cauley_Post-placement-needs-of-adoptive-mothers_1993.pdf
638ca7321f0101ee33df43eca9d22e44
https://thescholarship.ecu.edu/bitstream/10342/10341/2/Cauley_Post-placement-needs-of-adoptive-mothers_1993.pdf.txt
30c9de452a3148b85296a09dcd20e3eb
https://thescholarship.ecu.edu/bitstream/10342/10341/4/Cauley_Permissions_Yes.pdf
f7c759112b4354ba65abbffbaad769e5
NURS
oai:TheScholarship.intra.ecu.edu:10342/103952023-01-04T15:27:53Zcom_10342_73com_10342_122col_10342_117col_10342_124
A descriptive study of the role of the nurse in health promotion programs in the workplace throughout the United States
Singley, Jan Overman
Kirkpatrick, Mary K.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10395
https://thescholarship.ecu.edu/bitstream/10342/10395/1/Singley_A-descriptive-study-of-the-role_1986.pdf
76a32fa19dcb881e3727b0c7549273ef
https://thescholarship.ecu.edu/bitstream/10342/10395/2/Singley_A-descriptive-study-of-the-role_1986.pdf.txt
db7b06a04492e97d9b81193b92001f35
https://thescholarship.ecu.edu/bitstream/10342/10395/4/Singley_Permissions_Yes.pdf
a078c9960f894a3f55f0ce956a3e1a1d
NURS
oai:TheScholarship.intra.ecu.edu:10342/60522021-03-03T21:09:38Zcom_10342_122com_10342_2com_10342_1com_10342_107com_10342_73col_10342_124col_10342_8858col_10342_112
The Effectiveness of Interactive Metronome® as a Tool to Improve Cognition and Motor Performance in Healthy Older Adults in Eastern North Carolina
Reilly, Rebekah
Trujillo, Leonard
The purpose of this study was to measure the effectiveness of the Interactive Metronome (IM) in improving cognitive and motor performance in healthy older adults. As the aging adult population continues to rise, it is important to explore tools which can support this population to live as independently as possible, for as long as possible. Literature indicates there is a natural decline in cognition with aging, and that cognitive decline may be related to decline in functional performance. 13 healthy older adults completed 18 sessions of IM protocol and researchers gathered data from IM assessments, d2 Test of Attention, and Nine Hole Peg Test across four points of measure. Results indicated a significant increase in percentage of change from the baseline measure to the final point of measure on each of the four assessments. Researchers concluded the participants' improved scores on cognitive and fine motor measures may indicate IM could be beneficial in preventatively treating this population. Future research should explore if increased performance on assessment scores might contribute to increased functional performance.
East Carolina University
2016-12-15
Master's Thesis
en
http://hdl.handle.net/10342/6052
https://thescholarship.ecu.edu/bitstream/10342/6052/1/REILLY-MASTERSTHESIS-2016.pdf
9573ca44a8a29ff84f8fa0d43e4d20fd
https://thescholarship.ecu.edu/bitstream/10342/6052/2/LICENSE.txt
94fcd5802e3eac877c8f263881beaeb2
https://thescholarship.ecu.edu/bitstream/10342/6052/3/PROQUEST_LICENSE.txt
c03d879aaa0b9cd2a665dc78e3f4ce1c
https://thescholarship.ecu.edu/bitstream/10342/6052/4/Signature%20Sheet_Reilly.docx
b07c5b5c3b9ac134b408dca011c0f1fe
https://thescholarship.ecu.edu/bitstream/10342/6052/5/Signature%20Sheet.pdf
82c6591bd2c126c01e511f1ea60248ca
https://thescholarship.ecu.edu/bitstream/10342/6052/6/Signature%20Sheet_1.pdf
82c6591bd2c126c01e511f1ea60248ca
https://thescholarship.ecu.edu/bitstream/10342/6052/7/Non%20exclusive%20distribution.pdf
1d90fe43164b00ae61a1423e4884b497
https://thescholarship.ecu.edu/bitstream/10342/6052/8/NEDL.pdf
e81cd8776e8f977a7f6a60fc615c0f5f
https://thescholarship.ecu.edu/bitstream/10342/6052/9/NEDL_1.pdf
e81cd8776e8f977a7f6a60fc615c0f5f
https://thescholarship.ecu.edu/bitstream/10342/6052/11/REILLY-MASTERSTHESIS-2016.pdf.txt
1158831d6653514071072bd07e113f3f
Interactive Metronome
Aging Adults
Cognitive therapy
Psychomotor Performance
Adult
Cognition
Humans
North Carolina
Aged
oai:TheScholarship.intra.ecu.edu:10342/36002021-03-03T20:52:40Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Motor Learning Guided Treatment with Childhood Apraxia of Speech: Cueing & Feedback
Williamson, Sarah Elizabeth
Ball, Laura J.
The aim of this study was to determine if participants with childhood apraxia of speech (CAS) respond with improved speech production when provided motor learning guided (MLG) treatment strategies. Five participants, chronological ages 4;8 to 5;10 years, were provided three different types of treatment where cueing and feedback were systematically manipulated for six weeks. Treatment types included the following: verbal model with knowledge of performance feedback (VMKP), verbal model with knowledge of results feedback (VMKR), and visual model with knowledge of results feedback (KR). Each participant received 24 individual sessions, lasting approximately 15 minutes each for a total of 360 minutes. Following VMKP treatment, participants increased performance accuracy by an average of 13.4%. Following VMKR treatment, participants increased performance accuracy by an average of 4.8%. Finally, following KR treatment, participants increased performance accuracy by an average of 16%. All three treatment types produced positive outcomes; however, KR treatment resulted in the strongest positive outcome. The results of this study suggest that children with CAS may benefit from intervention where no verbal model is provided prior to speech practice and summary knowledge of results feedback is offered at intervals following 5 productions. Intervention in this study resulted in increased accuracy of speech performance and yielded optimal motor learning of those speech skills. Â
East Carolina University
2011
Master's Thesis
http://hdl.handle.net/10342/3600
https://thescholarship.ecu.edu/bitstream/10342/3600/2/Williamson_ecu_0600M_10362.pdf.txt
e6d64d25c3d646bb75ce8e767a46f0b2
https://thescholarship.ecu.edu/bitstream/10342/3600/1/Williamson_ecu_0600M_10362.pdf
b2f5692e491db77b8cf6c0b1d5439a71
https://thescholarship.ecu.edu/bitstream/10342/3600/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Childhood apraxia of speech
Cueing
Feedback
Motor learning
Speech intervention
Speech sound disorder
Apraxias
Rehabilitation of Speech and Learning Disorders
Speech Therapy
oai:TheScholarship.intra.ecu.edu:10342/64002021-03-03T21:15:21Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Targeting Kremen1 Downregulation with RVG-9R/siRNA Complexes in the Triple-Transgenic Mouse Model of Alzheimer’s Disease
Baker, Kelly E.
Murashov, Alexander K.
Alzheimer's disease (AD) is a progressive disease characterized by cognitive decline and memory loss. Memory loss observed in AD results from the loss of neurons and synapses which may be caused by the disruption of the canonical Wnt signaling pathway by Dickkopf-1 (Dkk-1). Under normal conditions, the canonical Wnt signaling pathway is responsible for normal neuronal development, synaptic plasticity, and overall normal brain function. Amyloid-[beta] (A[beta]) plaques and neurofibrillary tangles are two characteristic morphological changes observed in AD. An increased level of A[beta] has been associated with increased expression of Dkk-1, which may be linked to synaptic loss seen in AD. Kremen1 (Krm1) is a receptor for Dkk-1. Published and unpublished observations from our laboratory showed that silencing Krm1 with miR-431 can promote regenerative axon growth and prevent synaptic loss in a cell culture model. This study focused on downregulating Krm1 the triple-transgenic mouse model of AD (3xTg-AD). It was hypothesized that application of siRNA-431 in vivo would downregulate Krm1 thereby preventing synaptic loss and memory deficits in the 3xTg-AD mouse model of AD. Tail vein injections of RVG-9R/siRNA complexes and control injections were administered to 3xTg-AD mice and wild-type (WT) mice at 4, 6, or 12 months of age. Within each age cohort there were three different groups: 3xTg-AD mice injected with RVG/siRNA, 3xTg-AD mice injected with control peptide/siRNA, and WT mice injected with saline. Each group of mice was approximately half male and half female. Following the injections, the Barnes Maze was administered to each mouse in order to assess memory function. Data gathered from the Barnes Maze shows 3xTg-AD mice have a longer primary latency in the probe phase compared to WT mice. Of mice tested, fewer 3xTg-AD mice have been successful in finding the target hole during probe phase compared to WT mice. After completion of the Barnes Maze, mice were sacrificed and brains were collected for analysis. The brains were analyzed for Krm1 downregulation at the protein and mRNA levels via Western blot and qPCR, respectively. In 4 month old mice, WT mice showed the lowest levels of Krm1 protein and mRNA expression levels and 3xTg-AD CPep/siRNA treated mice showed the highest. The 4 month old 3xTg-AD RVG-9R/siRNA treated mice had Krm1 protein and mRNA expression levels that fell between the other two groups. Immunofluorescence was performed on coronal brain sections to analyze number of synapses. Six month old 3xTg-AD CPep/siRNA treated mice had significantly fewer synapses than both the WT and 3xTg-AD RVG-9R/siRNA treated groups. In conclusion, IF, qPCR, and Western blot data reveal the potential for RVG-9R/siRNA treatment to target and downregulate Kremen1 in vivo and provide protection from synaptic loss. However, further studies are need to confirm the ability of RVG-9R/siRNA treatment to downregulate Kremen1.
East Carolina University
2017-07-19
Master's Thesis
en
http://hdl.handle.net/10342/6400
https://thescholarship.ecu.edu/bitstream/10342/6400/1/BAKER-MASTERSTHESIS-2017.pdf
0a2fc27f0becfc4ea2cd52b98d5bf18a
https://thescholarship.ecu.edu/bitstream/10342/6400/2/LICENSE.txt
171663e6cdb606402e2fda76765fee74
https://thescholarship.ecu.edu/bitstream/10342/6400/3/PROQUEST_LICENSE.txt
b857219098972b01777f2c826aadfdfd
https://thescholarship.ecu.edu/bitstream/10342/6400/4/signature%20page.pdf
d82fe217e9e6885737f175bb18bb03eb
https://thescholarship.ecu.edu/bitstream/10342/6400/5/NEDL.KEB%20copy.pdf
1e12f0f9a9f543cad8085b0b8a784f90
https://thescholarship.ecu.edu/bitstream/10342/6400/7/BAKER-MASTERSTHESIS-2017.pdf.txt
542fd764e33112b2876db5ee08832ea6
Kremen1
Wnt Signaling
3xTg-AD Mouse Model
Alzheimer Disease
Down-Regulation
RNA, Small Interfering
Mice, Ttransgenic
oai:TheScholarship.intra.ecu.edu:10342/101302023-01-04T16:13:04Zcom_10342_73com_10342_122col_10342_117col_10342_124
Communicating with the patient requiring mechanical ventilation
Perkins, Ann
Duldt, Bonnie Weaver
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10130
https://thescholarship.ecu.edu/bitstream/10342/10130/1/Perkins_Communicating-with-the-patient-requiring-mechanical_1986.pdf
c7e8a1400db08b3b6c8201e0dfb940a8
https://thescholarship.ecu.edu/bitstream/10342/10130/2/Perkins_Communicating-with-the-patient-requiring-mechanical_1986.pdf.txt
e1a641f7ccf62eef4b55ded5f6436b3b
https://thescholarship.ecu.edu/bitstream/10342/10130/4/Perkins_Permissions_Yes.pdf
b5ae2b4bfc0a1c739134babd60a4f799
NURS
oai:TheScholarship.intra.ecu.edu:10342/43872021-03-03T20:55:12Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_39
Cost Comparison of Foods Purchased for an All-Organic Diet and a Conventional, Non-Organic Diet
Chestnut, Caroline Knauss
Myers, Kimberly B.
The objectives of this study were: To determine if there are mean cost differences between all-organic foods and conventional (non-organic) foods; to determine if there are differences in the mean cost of all-organic foods among higher, moderate, and lower price grocery venues; and to determine if the mean cost difference between all-organic and conventional foods varies among higher, moderate, and lower price grocery venues. The sample included selected organic food items and their conventional counterparts at a lower price (Walmart Supercenter), moderate price (Food City) and higher price (The Fresh Market) grocery venues in Kingsport, TN. Product price and package size in ounces or fluid ounces were collected. Cost per ounce was calculated for analysis. A repeated measures analysis of variance (ANOVA) with two within-subjects factors was used to determine statistically significant differences. A p value [less than or equal to] 0.05 was chosen as statistically significant. There was a significant main effect of organic status, F(1, 27) = 27.497, p < 0.001, for all foods e.g., food costs were significantly higher for organic foods compared with conventional foods. There was not a significant main effect of organic status in the Dairy group, F(1, 4) = 5.779, p = 0.074, though there was a trend towards significance since the p value was not much larger than 0.05. There was not a significant main effect of organic status in the Fruit group, F(1, 1) = 4.267, p = 0.287. There was a significant main effect of organic status in the Grain group, F(1, 8) = 10.318, p = 0.012; in the Protein group, F(1, 3) = 52.658, p = 0.005; and in the Vegetable group, F(1, 7) = 7.763, p = 0.027 e.g., food costs were significantly different for organic and conventional foods in the Grain group, Protein group, and Vegetable group. There was not a significant main effect of grocery venue, F(2, 54) = 0.664, p = 0.519, for all organic foods e.g., organic food costs were not significantly different among the lower price, moderate price, and higher price grocery venues. There was a significant interaction between the organic status and grocery venue, F(2, 54) = 8.633, p = 0.001 e.g., the difference in mean food costs between organic and conventional foods was significantly different among lower price, moderate price, and higher price grocery venues. It was found that organic foods were significantly more expensive than their conventional counterparts. Organic food costs were not influenced by grocery venue. Therefore an all-organic shopper may not significantly benefit by shopping for organic food at a lower price grocery venue. The differences in food costs between organic and conventional foods, however, were significantly different among grocery venues. Perceived cost increases between conventional and organic food items may depend on a chosen grocery venue. Further research is needed to analyze cost and availability of organic food items at various grocery venues including food cooperatives, superstores, health food stores, bargain grocers, and traditional national and local grocery stores.
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4387
https://thescholarship.ecu.edu/bitstream/10342/4387/1/Chestnut_ecu_0600O_11187.pdf
b7724ab7fef119e7b8f3173f0aa627c8
https://thescholarship.ecu.edu/bitstream/10342/4387/3/Chestnut.pdf
5c30db9cd7540b3d3ce89b2ce054f747
https://thescholarship.ecu.edu/bitstream/10342/4387/2/Chestnut_ecu_0600O_11187.pdf.txt
8145fb9ce92a2e50a15f20384957de58
Nutrition
Health
Organic
Natural foods--Prices
Food prices
oai:TheScholarship.intra.ecu.edu:10342/18132021-03-03T20:54:18Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Subjective and objective screening measures for dysphonia pre-thyroidectomy
Sanders, Ashley
Cox, Kathleen T.
There has been a link established between post-surgical dysphonia and thyroidectomy procedures and various studies identify that a large percentage of these patients have dysphonia after surgery. Most of these incidences of dysphonia resolve six months after the procedure, but there are reported cases of dysphonia that persists longer than six months and which are unrelated to any potential complications that could cause dysphonia. There may be a percentage of patients who present with dysphonia before surgery, which would indicate that the resilient dysphonia is related not to the surgery, but rather to their baseline vocal quality. The purpose of this study is to determine if there is a percentage of patients who are scheduled for thyroidectomies that have dysphonia and if there are any screening methods (subjective or objective) that surgeons may use to refer these potential cases of dysphonia for a full voice evaluation before the surgery. Eleven patients were included in this study. Each patient was a candidate for thyroidectomy and elected to have the procedure performed by Dr. Walter E. Pofahl, the cooperative surgeon for this study. For each patient, the following protocol was administered: background questions targeting factors that may indicate an increased risk of dysphonia, Voice Handicap Index, Consensus Auditory-Perceptual Evaluation of Voice, and Voice Evaluation Suite. This protocol was designed to measure present dysphonia subjectively, by gaining the patients' and the investigator's opinion of dysphonia, and objectively, through acoustic measurements. Graphical comparisons of the data revealed several "risk factors" surgeons could keep in mind and the CAPE-V may be a useful tool for pre-surgical referral. Â
East Carolina University
2013
Master's Thesis
http://hdl.handle.net/10342/1813
https://thescholarship.ecu.edu/bitstream/10342/1813/2/Sanders_ecu_0600M_10905.pdf.txt
127ac196662cce97598446602228a0dc
https://thescholarship.ecu.edu/bitstream/10342/1813/1/Sanders_ecu_0600M_10905.pdf
3714f862b582c8986ce46fa0ea201ade
Speech Therapy
CAPE-V
Dysphonia
Screening
Thyroidectomy
VES
VHI
Voice Disorders
Thyroidectomy--trends
oai:TheScholarship.intra.ecu.edu:10342/46622021-03-03T20:56:29Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
TMEFF2 is an epigenetic modulator that promotes androgen independent growth in castration-resistant prostate cancer cells
Corbin, Joshua Moses
Ruiz-Echevarria, Maria
While the ability to detect PCa has improved significantly due to PSA screenings, the survival rate for men diagnosed with PCa has remained stagnant, and the disease remains the second leading cause of cancer related deaths in men. Most patients initially respond to androgen deprivation treatment; however, a significant percentage of patients relapse with currently untreatable castration resistant prostate cancer (CRPC), during which the PCa cells develop the ability to grow in androgen depleted conditions. The androgen receptor (AR) plays a vital role in prostate development and homeostasis, and the deregulation of AR drives PCa tumorigenesis and progression to CRPC. Delineating molecular mechanisms that contribute to AR activity and/or PCa cell growth in androgen-depleted conditions may aid in the development of future CRPC therapies Epigenetic alterations play a critical role in differentiation during development, and aberrations in epigenetic regulation are associated with tumorigenesis and cancer progression. Two types of epigenetic modifications, DNA methylation and the methylation of multiple histone lysines, play significant roles in prostate cancer (PCa). Many histone methyltransferases (HMT) and demethylases (HDM), including the JMJD2 family of histone demethyalses, act as coregulators of AR, and many of these enzymes are implicated in CRPC. Because of this, HDMSs and HMTs have proven as attractive targets for therapeutic intervention. We have been studying TMEFF2, a protein that is regulated transcriptionally and translationally by the AR, and is overexpressed in PCa and CRPC suggesting a role in this disease. Data presented here demonstrate that TMEFF2 modulates JMJD2 controlled methyl histone marks and increases growth in androgen depleted conditions in CRPC cells. In correlation with its effect on histone methylation and growth, TMEFF2 overexpression increases resistance to the anti-growth effects of the pan-jumonji demethylase inhibitor, JIB-04, suggesting that TMEFF2 modulates growth, at least in part, by increasing jumonji demethylase activity. Additionally, TMEFF2 positively regulates PSA expression without altering AR levels in CRPC cells, indicating that TMEFF2 is a novel activator of AR. All together this data suggests a model in which TMEFF2, by modulating the activity of AR and JMJD2 enzymes, increases CRPC cell growth. Because CRPC remains to be a significant obstacle in the successful treatment of metastatic PCa, the results presented have the potential to be of therapeutic value.
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4662
https://thescholarship.ecu.edu/bitstream/10342/4662/1/Corbin_ecu_0600O_11285.pdf
a5c9a6b3510e8ec034a0f5866eb4c92e
https://thescholarship.ecu.edu/bitstream/10342/4662/2/CorbinNEDL.PDF
3ce8069d8bdd8bf992e81a832c3e896a
https://thescholarship.ecu.edu/bitstream/10342/4662/3/Corbin_ecu_0600O_11285.pdf.txt
456e742de0615419340064447e6fa4b8
Oncology
Prostatic Neoplasms--pathology
Prostatic Neoplasms--genetics
Prostatic Neoplasms, Castration-Resistant--pathology
Prostate--metabolism
oai:TheScholarship.intra.ecu.edu:10342/101572022-03-25T07:24:44Zcom_10342_73com_10342_122col_10342_117col_10342_124
A comparison of perceptions, benefits, advantages and convenience of breastfeeding between a group of Medicaid and non-Medicaid eligible women
Ketchie, Monica F.
Lieber, Marilyn Therese
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10157
https://thescholarship.ecu.edu/bitstream/10342/10157/1/Ketchie_A-comparison-of-perceptions-benefits-advantages_1994.pdf
992a7afebb9337ce9995360c03a40d5c
https://thescholarship.ecu.edu/bitstream/10342/10157/2/Ketchie_A-comparison-of-perceptions-benefits-advantages_1994.pdf.txt
0c60d73cc6b783a710d5a39ee8f2ee35
NURS
oai:TheScholarship.intra.ecu.edu:10342/46392021-03-03T20:56:19Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Temporal stability of the electronic version of the Motor-Free Visual Perception Test third edition (MVPT-3)
Eanes, Andrea Sparks
Trujillo, Leonard
In today's technology based society, children are learning information from digital devices and are currently being tested by paper assessments. New electronic assessments have been created to try to compensate for this discrepancy. However, very few of these electronic assessments have been tested to see if they are a reliable measure. The electronic version of the Motor-Free Visual Perception Test 3rd edition (MVPT-3) is a recently developed assessment that has not been assessed to see if it is a reliable measure. The purpose of this study sought to determine if the electronic version of the MVPT-3 is reliable assessment to use with children ages 4 years 0 months to 10 years 11 months. A test-retest correlational design was used to assess temporal stability. The main research questions reviewed if multiple administrations of the Electronic Version of the MVPT-3 yields consistent outcome scores and if the electronic version of the MVPT-3 is reliable for professionals to use clinically. Fifty-four participants were recruited from four daycare centers and afterschool programs in Eastern and Central North Carolina and ranged in ages 4 years 0 months to 10 years 11 months. Complete data was collected on forty-eight participants and was analyzed via a single tailed Pearson product-moment correlation. The results indicated that the electronic version of the MVPT-3 presents clinically acceptable test-retest reliability with a correlation coefficient of r=.81. Learning effects were revealed for certain items. Results indicate that this is an appropriate screening assessment for school aged children but should not be used for diagnostic purposes. The results also demonstrate that further and more extensive research needs to be conducted in order to determine if the electronic version of the MVPT-3 is a reliable measure of visual perception.
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4639
https://thescholarship.ecu.edu/bitstream/10342/4639/1/Eanes_ecu_0600O_11328.pdf
a2ed2130d761bfbcb6f1a9d2860f3147
https://thescholarship.ecu.edu/bitstream/10342/4639/2/Eanes_NEDL.pdf
3f40b201c07b63c523ad7fd1e4cda722
https://thescholarship.ecu.edu/bitstream/10342/4639/3/Eanes_ecu_0600O_11328.pdf.txt
4325e81bf2acd52b06593b5111bb2e4f
Occupational Therapy
Electronic
MVPT-3
Visual perception--Testing
Visual Perception--physiology
Occupational Therapy--methods
oai:TheScholarship.intra.ecu.edu:10342/100492023-01-04T16:18:42Zcom_10342_73com_10342_122col_10342_117col_10342_124
Identifying the roles, responsibilities, and practices of nurses in telehealth/telemedicine programs
Horton, Maria Chahl
Trought, Elizabeth A.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10049
https://thescholarship.ecu.edu/bitstream/10342/10049/1/Horton_Identifying-the-roles-responsibilities-and-practices_1996.pdf
09f62dd13fb5ac7a9055f398ca6c0ca6
https://thescholarship.ecu.edu/bitstream/10342/10049/2/Horton_Identifying-the-roles-responsibilities-and-practices_1996.pdf.txt
0c5b018bb8ee58a50006289418d69269
https://thescholarship.ecu.edu/bitstream/10342/10049/4/Horton_Permissions_Yes.pdf
b2e9048a64007f10fd5f525e22592bc6
NURS
oai:TheScholarship.intra.ecu.edu:10342/35992021-03-03T20:52:41Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Schedule Distribution and Motor Learning Guided Treatment with Childhood Apraxia of Speech
Webb, Caitlin L.
Ball, Laura J.
The purpose of this study was to examine the use of motor learning guided (MLG) treatment with different treatment schedules in the treatment of participants with a diagnosis of childhood apraxia of speech (CAS). Five participants, chronological ages 4;6 to 5;11 years, received MLG treatment for diagnosed CAS in two different treatment schedules, mass and distributed. The mass schedule consisted of four weekly 60-minute treatment sessions for a total of 240 minutes of intervention. The distributed schedule consisted of 16 15-minute treatment sessions provided four days a week for a total of 240 minutes of intervention. With the mass treatment schedule, participants demonstrated an increase in performance accuracy by an average of 9.1%. With the mass treatment schedule, participants demonstrated an increase in probe accuracy by an average of 5%. With the distributed treatment schedule, participants demonstrated an increase in performance accuracy by an average of 21.4%. With the distributed treatment schedule, participants demonstrated an increase in probe accuracy by an average of 17%. Both treatment schedules produced positive outcomes with the distributed treatment schedule resulting in the highest improvement in speech production accuracy. The results of this study suggest that children with CAS may benefit from shorter and more frequent intervention sessions to yield motor learning of speech skills and to increase accuracy of speech production performance. Â
East Carolina University
2011
Master's Thesis
http://hdl.handle.net/10342/3599
https://thescholarship.ecu.edu/bitstream/10342/3599/2/Webb_ecu_0600M_10384.pdf.txt
6f6e56ea4f07a4781f337f8d430a8254
https://thescholarship.ecu.edu/bitstream/10342/3599/1/Webb_ecu_0600M_10384.pdf
10f1899c343a5132fdcc49aa803c68c1
https://thescholarship.ecu.edu/bitstream/10342/3599/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Pediatrics
Practice distribution
Speech impairment
Treatment
Apraxias
Speech
Speech Therapy
oai:TheScholarship.intra.ecu.edu:10342/95522022-03-29T14:04:05Zcom_10342_74com_10342_73col_10342_106oai:TheScholarship.intra.ecu.edu:10342/47652021-03-03T20:57:24Zcom_10342_73com_10342_1col_10342_117col_10342_72
An Evaluation of Referral and Follow-Up Care for Postpartum Home Visitation Recipients
Caddell, Lindsay E.
Larson, Kim L.
A collaborative partnership was developed with a local Health Department, specifically the Postpartum Home Visitation (PPHV) program in the county and a senior nursing student at East Carolina University College of Nursing. The goal of the county PPHV program is to assess both mother and infant within two weeks following delivery. Public health nurses complete assessments and make referrals to essential community resources during this visit. Postpartum home visits often result in referrals to health department services or other community resources. Public health nurses, social workers, case managers and interpreters work together on referral and follow-up. There was no current comprehensive tracking system to monitor client progression through the referral and follow-up process. Therefore, the overall goal of this project was to evaluate the referral and follow-up process to community resources for the recipients of the PPHV program.
2015
Honors Thesis
http://hdl.handle.net/10342/4765
https://thescholarship.ecu.edu/bitstream/10342/4765/1/Caddell%20FINAL%20SHP.doc
ace165b2484686cdbbcdd3f0eb1709bf
https://thescholarship.ecu.edu/bitstream/10342/4765/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/4765/3/Caddell%20FINAL%20SHP.doc.txt
6e81f2b39a7a59cbd345aed851e761c8
Postpartum home visitation
Postpartum referral
Home visitation follow-up
oai:TheScholarship.intra.ecu.edu:10342/35982021-03-03T20:52:40Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Use of Oral Language General Outcome Measures to Describe Children According to Language Status
Strein, Alicia
Ball, Laura J.
The purpose of the study was to determine if short language samples can effectively distinguish children with language impairments from those who are typically developing. Four elicitation methods (individual picture descriptions, multi-scene picture description, narrative, and interview) were administered to 14 typically developing children and 3 children with language impairments in Chapel Hill, NC as well as to 16 typically developing children in Greenville, NC. Each task was administered 3 times and lasted 1 minute. All samples were collected and transcribed by the principal investigator and then analyzed using 5 general language sample measures (Number of Total Words, Number of Different Words, Number of Total Utterances, Mean Length of Utterance, Errors/Omissions). Intercorrelations between the elicitation tasks were found for each measure and then the effect size was determined comparing the typically developing children and children with language impairments in Chapel Hill, NC. Significant correlations were observed between most measures. A review of the correlation strength revealed the strongest relationships between the descriptions of individual pictures and multi-scene picture description tasks. Notable differences were observed between children with language impairments and typically developing children. The largest group differences were observed for the Number of Different Words measure. The results suggest that the short language samples produce meaningful information and are clinically feasible. Â
East Carolina University
2011
Master's Thesis
http://hdl.handle.net/10342/3598
https://thescholarship.ecu.edu/bitstream/10342/3598/2/Strein_ecu_0600M_10372.pdf.txt
3bde5197b4f7eeebae30502746aa6a8e
https://thescholarship.ecu.edu/bitstream/10342/3598/1/Strein_ecu_0600M_10372.pdf
e8cbec1aceb9e8d3bd7baacd4e17b055
https://thescholarship.ecu.edu/bitstream/10342/3598/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Curriculum based measures
General outcome measures
Language sample analysis
Oral language
Language Development
Child Development
Speech Therapy
oai:TheScholarship.intra.ecu.edu:10342/60562021-03-03T21:09:34Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Effects of the Interactive Metronome® on older adults : does use improve cognitive and motor abilities?
Christy, Lauren S
Trujillo, Leonard
The purpose of this pre-test, post-test, quasi-experimental study was to examine the relationship between the Interactive Metronome[registered] (IM) protocol and cognitive plasticity, cognitive retention, and motor skills of older adults in southeastern North Carolina. The IM protocol was carried out in various settings with an occupational therapist and/or graduate occupational therapy students present for motivation and guidance. All participants underwent pre- and post-test assessments in order to track cognitive and motor function as they moved through the protocol. Cognitive assessments included the Woodcock-Johnson III (WJ III) and the d2 Test of Attention (d2). Motor assessments included the Four Step Square Test (FSST) and the Nine Hole Peg Test (NHPT). The participants' scoring and progress within the IM program was also tracked and examined for trends and indicators. This pre-test, post-test, quasi-experimental design was used to compare the differences between the assessment scores before and after intervention with the IM protocol. In all, a relationship was delineated between positive percentage of change on IM performance and positive percentage of change on the cognitive and motor assessments.
East Carolina University
2016-12-15
Master's Thesis
en
http://hdl.handle.net/10342/6056
https://thescholarship.ecu.edu/bitstream/10342/6056/1/CHRISTY-MASTERSTHESIS-2016.pdf
f57c17fab4dcf58d0fca5494394c0f85
https://thescholarship.ecu.edu/bitstream/10342/6056/2/LICENSE.txt
d9cd2fa944e98cc396fc912467a31781
https://thescholarship.ecu.edu/bitstream/10342/6056/3/PROQUEST_LICENSE.txt
50f9d7c0af91a821da930541711025d6
https://thescholarship.ecu.edu/bitstream/10342/6056/4/Committee_Signatures.docx
3fb30dac5b749607634181c6bdd06bb7
https://thescholarship.ecu.edu/bitstream/10342/6056/5/SignaturePage_Signed.pdf
b524367be0b2dfab53357eab686f3258
https://thescholarship.ecu.edu/bitstream/10342/6056/6/SignaturePage_Signed_1.pdf
b524367be0b2dfab53357eab686f3258
https://thescholarship.ecu.edu/bitstream/10342/6056/7/Non-Exclusive%20Distribution%20License.pdf
932bf50afa6a40564a13fe50f68ac489
https://thescholarship.ecu.edu/bitstream/10342/6056/8/NEDL_signed.pdf
191ebaaf0853141d26fd5c25e0fb97a0
https://thescholarship.ecu.edu/bitstream/10342/6056/10/CHRISTY-MASTERSTHESIS-2016.pdf.txt
f4e97d2d9a55b87cb0a20c47e3d1e338
Interactive Metronome
Healthy Older Adults
Aging In Place
Quality of Life
Cognition
Motor Skills Disorders
North Carolina
Aged
Cognitive therapy
oai:TheScholarship.intra.ecu.edu:10342/103052023-01-04T16:26:01Zcom_10342_73com_10342_122col_10342_117col_10342_124
An investigation of the adherence behavior and adverse effects of pregnant women on supplemental iron
Williams, Sandra L.
Bergstrom, Linda
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10305
https://thescholarship.ecu.edu/bitstream/10342/10305/1/Williams_An-investigation-of-the-adherence-behavior_1994.pdf
a4ac8d7d75188a1e48a5836a508e1987
https://thescholarship.ecu.edu/bitstream/10342/10305/2/Williams_An-investigation-of-the-adherence-behavior_1994.pdf.txt
67395fefde4a63a998e7bb5eb00e8493
https://thescholarship.ecu.edu/bitstream/10342/10305/4/Williams_Permissions_Yes.pdf
146bc2bd37eaace4bc0912c51ff23cb7
NURS
oai:TheScholarship.intra.ecu.edu:10342/65572022-09-29T13:41:08Zcom_10342_73com_10342_1col_10342_117col_10342_72
Indigenous Ways of Explaining Health and Illness
Davis, Morgan
Larson, Kim
The purpose of this qualitative descriptive study was to examine indigenous ways of explaining health and illness from the perspectives of Mayans in one rural village in Guatemala. A bilingual research team conducted interviews with 10 Maya adults (nine female and one male). Interviews were conducted in Spanish, audiotaped, and transcribed from Spanish to English. A native-speaker validated all interviews.
Content analysis was used to identify commonalities and differences in explaining health and illness. Health was explained as eating healthy foods and drinking filtered water. All participants reported the use of herbs contributing to health and in treating illness. Herbs used were those grown on el monte/the mountain. Illness was explained by changes in behavior, such as sadness, lethargy, and a lack of appetite. Participants used the puesto de salud/health post and family members for advice on health and illness. Only one participant reported contact with a traditional healer for illness care.
A Mayan explanation of health coincides with the Sustainable Development Goals (2017) of food security and access to safe drinking water. For many families living in Guatemala these basic necessities come only as a result of global partnerships. The puesto de salud is a valuable community resource that may benefit from global partnerships to build capacity and sustain resources.
East Carolina University
2017-12-11
Honors Thesis
http://hdl.handle.net/10342/6557
https://thescholarship.ecu.edu/bitstream/10342/6557/1/DAVIS-HONORSTHESIS-2017.pdf
14072c7ea6cab971b31382d7877083f7
https://thescholarship.ecu.edu/bitstream/10342/6557/2/LICENSE.txt
8e71d3fa43b741f0a3c5b69dbffb6e8b
https://thescholarship.ecu.edu/bitstream/10342/6557/4/DAVIS-HONORSTHESIS-2017.pdf.txt
2c3c9a8e4efbe24edb87f709f5420175
Maya, indigenous, health, illness, culture
oai:TheScholarship.intra.ecu.edu:10342/40632021-03-03T20:52:59Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Carry-over effect of a handwriting readiness program on handwriting-related skills of children the year following intervention
Lear, Whitney Shay
Donica, Denise
The purpose of this follow-up study was to determine if children who had participated in an occupational therapy based handwriting readiness program would show greater improvements in handwriting-related skills a year following intervention when compared to a control group and an alternate experimental group. The entire study (initial study and follow-up study) was a time series longitudinal design with 4 data collection points. Sixteen children (4 from the control group, 6 from the experimental group, and 6 from the alternate experimental group) were tested in September 2010, received intervention, and were again tested in March 2011 during the initial study. This follow-up study then included 2 more post-testing sessions in September 2011 and in March 2012. Testing sessions included the Beery-Buktenica Developmental Test of Visual-Motor Integration - Sixth Edition (VMI) and four of the eight subtests from the Bruininks-Oseretsky Test of Motor Proficiency - Second Edition (BOT-2). All children completed all testing at the first session of the initial study in September 2010, participated in the intervention during the initial study, and completed all tests at both testing sessions for this follow-up study in September 2011 and in March 2012. (At the second testing session in March 2011, 2 children did not complete the Upper-Limb Coordination Subtest of the BOT-2 and 1 child did not complete the Fine Motor Integration Subtest or the Fine Motor Precision Subtest of the BOT-2). The dependent variables were the scores received on the VMI and the Fine Motor Precision Subtest, Fine Motor Integration Subtest, Manual Dexterity Subtest, and Upper-Limb Coordination Subtest of the BOT-2. The independent variable was the handwriting instruction program in which the child participated during the initial study. Data analysis indicated that children who participated in the Fine Motor and Early Writing (FMEW) Pre-K curriculum (experimental group) showed greater improvements in median scores on the BOT-2 Fine Motor Precision and Manual Dexterity subtests from the end of the intervention year to one year following intervention when compared to the control group and the alternate experimental group. Both the VMI and the Fine Motor Integration and Upper-Limb Coordination subtests of the BOT-2 showed the control group with the greatest median change in scores. It is difficult to draw conclusions from the results of this study, as limitations including a lack of randomization between the three groups leading to considerable differences in age and gender strongly affected results, leading to inconclusive data about the effects of the FMEW curriculum on handwriting-related skills of children one year following intervention.
East Carolina University
2012
Master's Thesis
http://hdl.handle.net/10342/4063
https://thescholarship.ecu.edu/bitstream/10342/4063/1/Lear_ecu_0600M_10830.pdf
dce963e62ea1d26d37346cba3fa12954
https://thescholarship.ecu.edu/bitstream/10342/4063/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/4063/3/Lear_ecu_0600M_10830.pdf.txt
0206fac1425f7d4b42530cc103133652
Early childhood education
Fine motor and early writing pre-K curriculum
Handwriting
Handwriting without tears
Head Start
Occupational Therapy
Occupational Therapy--methods
Early Intervention (Education)
Learning
Psychomotor Performance
Motor Skills--physiology
Treatment Outcome
oai:TheScholarship.intra.ecu.edu:10342/44722021-03-03T20:56:42Zcom_10342_1com_10342_74com_10342_73col_10342_72col_10342_93
Relationship Between Cardiovascular Disease Risk and Fast Food Consumption in Undergraduate Honors College Students
Maidah, Atta N.
Lai, Hsiao L.
College students are often pushed towards fast foods as a means of getting a pleasurable, quick bite to eat. Fast foods are high in fat, salt, and sugar content and can lead to cardiovascular disease(CVD) risk. Honors students may be more conscious of negative effects of bad dietary choices. Objectives include assessing Honors students knowledge of American Dietary Association nutritional recommendations and analyzing association between fast food consumption and CVD risk factors: physical inactivity, stress level, BMI, and family history of CVD and CVD risks. An 18-question survey was administered between April and June 2013 to East Carolina University Honors College students via the Honors College list serve. The survey measured frequency of fast food, sweetened beverage and snack consumption; height, weight, exercise frequency, stress levels, course load, and family history of CVD, and nutritional knowledge. Students were categorized as frequent fast food consumers (FFF) 3 or more times a week or infrequent fast food consumers (IFF) less than 3 times a week. 92 responses representing 36.2% of the 2012-2013 Honors College were collected. Twenty-three percent of participants were male, 79.3% White, 13% Asian, 5.4% Black descent, and 2% Other. Thirty-seven percent were able to correctly identify their nutritional recommendations. IFF were able to answer 2 out of 3 nutritional questions correctly versus only 30% of FFF. A fifth(21.7%) of participants had 3 or more CVD risks. Thirty percent of FFF had 3 or more cardiovascular disease risk factors versus 18.8% of IFF. Frequent fast food consumers had more risk factors compared to IFF. Students who answered nutritional questions correctly also consumed less fast food, demonstrating that they applied their knowledge to their own eating habits.
2014
Undergraduate Thesis
http://hdl.handle.net/10342/4472
https://thescholarship.ecu.edu/bitstream/10342/4472/2/Atta_Maidah%20%281%29.pdf
9ab72637acd2d5dd1eac37af943c8d02
https://thescholarship.ecu.edu/bitstream/10342/4472/3/Atta_Maidah%20%281%29.pdf.txt
cdfb4df04c3c29ab021e841c51881b53
Fast food
Cardiovascular Diseases
Honors college students
CVD risk
Food consumption
oai:TheScholarship.intra.ecu.edu:10342/104292022-12-13T15:16:00Zcom_10342_73com_10342_122col_10342_117col_10342_124
Modes of coping with anger in individuals with schizopnrenia
Currin, Candace T.
Duldt, Bonnie Weaver
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10429
https://thescholarship.ecu.edu/bitstream/10342/10429/1/Currin_Modes-of-coping-with-anger-in_1987.pdf
b906abe917aa10808f230c0c2d595421
https://thescholarship.ecu.edu/bitstream/10342/10429/2/Currin_Modes-of-coping-with-anger-in_1987.pdf.txt
1f92c68ba73c878366765eeb8d585be6
NURS
oai:TheScholarship.intra.ecu.edu:10342/87162021-10-05T14:43:00Zcom_10342_73com_10342_122col_10342_117col_10342_123
METHODS OF IDENTIFYING NEUROLOGICAL DELIRIUM (MIND) STUDY
Mulkey, Malissa Ann
Gantt, Laura, 1957-
Delirium is a major concern for critically ill older patients because it is associated with significant risk to quality of life and mortality. The United States spends approximately $164 billion in Medicare funding annually to combat the consequences of delirium. This figure is only expected to increase as the number of individuals aged 65 years or older increases. By 2030, one in four individuals in the United States will be 65 years old or older. Of further concern is that more adults are surviving critical illness and are therefore living with more comorbidities. Currently, as many as 80% of adults in intensive care units develop delirium. Unfortunately, healthcare providers fail to recognize 80% of these cases. Delirium is an acute fluctuating disorder impacting attention and global cognitive function with an underlying organic cause. As the brain attempts to adapt to overwhelming stress, reductions in cortical activity result in neurotransmitter imbalances. These imbalances are reflected electrographically on electroencephalogram (EEG). Once the ability to compensate has been exhausted, behavioral symptoms associated with delirium begin to appear. Standardized assessments for delirium need to be capable of early, accurate, and objective identification. EEG is the gold standard for delirium detection but is not always feasible due to cost, technical setup, and need for skilled interpretation. Currently available instruments, while effective in the clinical research setting, have not translated well into practice. As a result, they fall short of accurate detection for a variety of reasons, including intermittent and retrospective data collection and requiring examiner interpretation. The lack of objective physiological monitoring capability for delirium detection prevents nurses and other healthcare providers from proactively managing this debilitating clinical problem. Because nurses provide frontline care to this patient population, the lack of adequate methods for detection presents a gap in nursing science. Recently developed technology, signal processed limited lead EEG, may provide an alternative to traditional monitoring methods for delirium. Limited lead EEG can provide the EEG waveform information needed to determine delirium status, is much cheaper than traditional EEG and can be applied by nurses, thereby overcoming limitations seen with traditional EEG such as cost and technical set-up. Critical care nurses currently use this type of EEG for sedation titration and monitoring. Because these monitors can analyze the most reliable biomarker, EEG, they may provide much needed objective, accurate, early identification of delirium. The design for this study was prospective exploratory and cross-sectional. After appropriate Institutional Review Board permissions, a convenience sample of patients were recruited from the cardiac, medical and surgical intensive care units in a large southeastern academic medical center. Study data were collected by the author and prepared for analysis. Analytical methods included descriptive statistics, statistical methods to compare groups, and statistical methods to explore relationships among variables. Study results were then described and discussed.
East Carolina University
8/5/2020
Master's Thesis
http://hdl.handle.net/10342/8716
https://thescholarship.ecu.edu/bitstream/10342/8716/1/Mulkey_Malissa_Dissertation_07.14.20.pdf
959dc77defc58a621b16e22e538034ae
https://thescholarship.ecu.edu/bitstream/10342/8716/2/Mulkey_Malissa_NonExclusive_Distribution_License_July%202020_Complete.pdf
b754d3aeca42ce8919a4c8d389b97ff6
https://thescholarship.ecu.edu/bitstream/10342/8716/3/Mulkey_Malissa_Signature%20Page_July%202020.pdf
a78df78173628ff07b06c1e47c67f129
https://thescholarship.ecu.edu/bitstream/10342/8716/4/Mulkey_Malissa_Dissertation_07.14.20.pdf.txt
10dd2c712a11b1423e945a8101b37b0d
Delirium
Risk factors
oai:TheScholarship.intra.ecu.edu:10342/52352022-03-29T14:02:38Zcom_10342_30com_10342_1com_10342_74com_10342_73col_10342_31col_10342_106oai:TheScholarship.intra.ecu.edu:10342/102432022-03-25T07:31:13Zcom_10342_73com_10342_122col_10342_117col_10342_124
The meaning of illness for breast cancer patients and their spouses
Roberson, Donna W.
Kirkpatrick, Mary K.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10243
https://thescholarship.ecu.edu/bitstream/10342/10243/1/Roberson_The-meaning-of-illness-for-breast_1993.pdf
5fe1bd2af7051c410816d1ba86c7a124
https://thescholarship.ecu.edu/bitstream/10342/10243/2/Roberson_The-meaning-of-illness-for-breast_1993.pdf.txt
93dbb444c61d65897308c654e2f4ea87
NURS
oai:TheScholarship.intra.ecu.edu:10342/94582022-12-12T18:52:55Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Using Eye Tracking Technology To Compare Hazard Detection On Road Versus Driving Simulator At Night Across Two Age Groups
Leonardo, Juliette
Dickerson, Anne Elizabeth
Rationale: Though previous literature exists that demonstrates the validity of using driving
simulation compared to on-road driving, few studies have examined hazard detection between
two driving conditions. Even fewer studies have specifically examined hazard detection at night.
Results of current research regarding age differences in driving is inconclusive, and night driving
is an under-researched area. Purpose: This study sought to analyze the visual components of
night driving for older adults. Eye tracking technology and driving simulation were combined to
analyze on-road scanning behavior, visual attention, glance patterns, and hazard detection.
Research questions sought to determine if there were significant differences in hazard detection
between: 1) on-road and simulated drives, 2) older adults and younger adults, or any interaction
effect. Design: This quasi-experimental study used a 2x2 repeated-measures factorial design and
examined comparisons between two age groups (older adults v. younger adults) and across two
driving situations (on-road v. simulated). Driving conditions were counterbalanced.
Participants: Participants included 16 older drivers (65+ years) and 17 younger drivers (20-40
yeras). All participants were healthy, community-living adults obtained through convenience
sampling. Methods: Instruments included wearable Tobii Pro eye tracking glasses which tracked
and recorded pupil glances, a STISIM driving simulator, and each participant’s personal vehicle.
In both the on-road and simulated conditions, a pedestrian “hazard� stood at three locations.
Pedestrians stared at their cell phone and appeared to cross the street, though they did not
actually walk. Outcomes examined from the eye tracking recordings in the Tobii Pro Analysis
software included total fixation duration, fixation count, and time to first fixation. Analysis: The
majority of findings indicate that night hazard detection behavior was similar across the driving
conditions with the exception of time to first fixation. Time to detect the hazards was generally
quicker on the road in both age groups. Comparison of the eye tracking measures indicated few
statistically significant differences between older and younger adults’ hazard detection behavior.
Though, older adults did take slightly longer to initially see hazards. Discussion: Findings
indicated that, despite age-related visual decline, older adults detected hazards similarly to
younger adults, especially when assessing on-road performance. However, they may take slightly
longer to see hazards at night which should be considered in self-regulation. Results also support
the use of driving simulators as a safe mechanism to observe driving habits, behaviors, and
mistakes without risk to the driver, evaluator, or environment. Though, fitness to drive
assessment should also include on-road observation due to limitations in absolute validity of
simulation. Future research should place emphasis on analysis on time to first fixation, as it may
offer the most driving-safety related information. Occupational therapists have a vital role in
determining/addressing fitness to drive, reducing risk of crashes, and researching ways to address
occupational needs.
2021-05-01
Thesis
en_US
http://hdl.handle.net/10342/9458
https://thescholarship.ecu.edu/bitstream/10342/9458/1/USING%20EYE%20TRACKING%20TECHNOLOGY%20TO%20COMPARE%20HAZARD%20DETECTION%20ON%20ROAD%20VERSUS%20DRIVING%20SIMULATOR%20AT%20NIGHT%20ACROSS%20TWO%20AGE%20GROUPS.pdf
d6e0e6ab43230fada875416e62af01ca
https://thescholarship.ecu.edu/bitstream/10342/9458/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/9458/3/Signature%20Page%20Leonardo.pdf
5cc1261931328518951d6dc023a6a4fa
https://thescholarship.ecu.edu/bitstream/10342/9458/4/JLeonardoNEDL.pdf
27725d7b97b826e0626b9642182c6991
https://thescholarship.ecu.edu/bitstream/10342/9458/5/USING%20EYE%20TRACKING%20TECHNOLOGY%20TO%20COMPARE%20HAZARD%20DETECTION%20ON%20ROAD%20VERSUS%20DRIVING%20SIMULATOR%20AT%20NIGHT%20ACROSS%20TWO%20AGE%20GROUPS.pdf.txt
5f812324600d888891fa1171389ba59f
Eye Tracking Technology
Driving
Hazard Detection
Night Driving
oai:TheScholarship.intra.ecu.edu:10342/44232021-03-03T21:10:45Zcom_10342_122com_10342_74com_10342_73col_10342_124col_10342_100
Paternal diet and exercise epigenetically program energy expenditure and glucose metabolism in mouse offspring
Koury, Michael
Murashov, Alexander K.
It is currently estimated that a third of Americans suffer from metabolic syndrome, which is an obesogenic disease shown to increase risk for developing type 2 diabetes (T2DM) by five-fold (Diabetes Care, 2012). The pervasiveness of obesity and T2DM is largely the result of unbalanced nutrition and lack of physical activity. Epidemiological investigations are now finding the detrimental effects of poor lifestyle choices, such as consuming a high-fat diet, have heritable consequences that persist transgenerationally by way of epigenetic modifications. The epigenome is yet another tier of genetic regulation, one that is capable of producing heritable changes in gene expression without altering the underlying sequence of nucleotides. In an effort to better understand the pathogenesis of these overly prevalent diseases (i.e. obesity and T2DM) researchers have now set their sights on faulty epigenetic machinery. To date, research has primarily focused on epigenetic modifications associated with maternal descent, mainly because of the significant influence had by lifestyle and the environment during 9-months in utero. Consequently, the roles of paternal lifestyle choices, like diet and activity, have not received adequate attention. Using a C57BL/6J mouse model we examined the transgenerational effects of a paternal high fat-diet and prolonged exercise on male offspring's susceptibility to glucose intolerance. Founder fathers (F₀) were randomly divided into three groups: control-diet fathers (CF) // high-fat fathers (FF); 60% of energy derived from fat // control-diet fathers with exercise wheel (EF). After 12 weeks of a high-fat diet (HFD) or free-wheel running F₀ males from each group were mated with control females. At 4 weeks of age their male offspring (F�) were assigned to either a HFD or control diet (CD; 10% of energy derived from fat) for a duration of 12 weeks. Metabolic profiles were assessed via indirect calorimetry (i.e. metabolic cages), glucose tolerance testing (GTT), fasting plasma insulin, eMRI imaging (fat/lean body composition), as well as monitoring of developmental milestones. A comparison between cohorts of offspring on post-natal day 7 revealed a significantly lower mean birth weight in the HFD-father offspring (FFO); a factor shown in humans to be predictive of obesity and impaired glucose tolerance in adulthood (Bhargava et. al. 2004). Additionally, when challenged with a HFD only exercise father offspring (EFO) exhibited diabetic traits such as fasting hyperglycemia, fasting hyperinsulinemia, lower energy expenditure, as well as increased body weight and adiposity. To better understand the molecular mechanisms driving these observations quantitative real-time PCR was utilized to examine the methylation profile and gene expression within insulin sensitive tissues like the liver, pancreas, and gastrocnemius muscle. Data show intriguing differences in expression of several metabolic genes such as: Ogt, Oga, Pdk4, Glut4, Ptpn1, Igf2, H19, and FoxO1. Furthermore, with the exception of Glut4, methylation patterns in fathers were preserved within male offspring. These novel findings suggest that offspring have a phenotype that is epigenetically programmed to thrive under the same experimental conditions as their respective fathers.
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4423
https://thescholarship.ecu.edu/bitstream/10342/4423/2/Koury_ecu_0600O_11122.pdf.txt
ba942a51ce76be9d30be655c52138046
https://thescholarship.ecu.edu/bitstream/10342/4423/3/Koury.pdf
eb54c0edbf2062663b565ce7a9caca38
https://thescholarship.ecu.edu/bitstream/10342/4423/1/Koury_ecu_0600O_11122.pdf
cc7a6cac2b4abdaaf3df6e7a31d60b5c
Physiology
Genetics
Public health
Diabetes
Energy
Epigenetics
Metabolism
Obesity
O-GlcNAc
Biology, Genetics
Biology, Physiology
Metabolic Syndrome X--metabolism
Diabetes Mellitus, Type 2--metabolism
Diet, High-Fat
oai:TheScholarship.intra.ecu.edu:10342/104502022-03-29T07:22:29Zcom_10342_73com_10342_122col_10342_117col_10342_124
Dissatisfaction with telephone communications with physicians among nurses in two nursing home settings
McDaniel, James Goodlett
Vincent, Pauline
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10450
https://thescholarship.ecu.edu/bitstream/10342/10450/1/McDaniel_Dissatisfaction-with-telephone-communications-with-physicians_1985.pdf
2ba4b710a6d282510d3b5163676f6109
https://thescholarship.ecu.edu/bitstream/10342/10450/2/McDaniel_Dissatisfaction-with-telephone-communications-with-physicians_1985.pdf.txt
563d4bc46509ba681cbf714d1ebffa08
NURS
oai:TheScholarship.intra.ecu.edu:10342/104422022-03-29T07:19:40Zcom_10342_73com_10342_122col_10342_117col_10342_124
An assessment of level of knowledge about individual blood pressure values and the disease of hypertension among residents of eastern North Carolina
Edwards, Sue G.
Rose, Mary Ann
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10442
https://thescholarship.ecu.edu/bitstream/10342/10442/1/Edwards_An-assessment-of-level-of-knowledge_1985.pdf
ec82721b90cc162b4f938339da869f40
https://thescholarship.ecu.edu/bitstream/10342/10442/2/Edwards_An-assessment-of-level-of-knowledge_1985.pdf.txt
f8b5f0e1f278f3955a0b6618f7ed02cd
NURS
oai:TheScholarship.intra.ecu.edu:10342/43412021-03-03T20:54:00Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
A case study of occupational therapists serving military personnel: identifying therapeutic approaches to be considered when assessing the occupational performance of military service members with mild traumatic brain injury
Pippin, Kelly
Trujillo, Leonard
The purpose of this study was to explore the means by which occupational therapists experienced in assessing the occupational performance of military service members (MSMs) with mild traumatic brain injury (mTBI) utilize available assessment tools and techniques to conduct assessments with regards to the uniqueness of military culture.   Qualitative data was collected through face-to-face and phone interviews with three occupational therapists who have experience working with MSMs with mild traumatic brain injury as a primary client base. Written follow up questionnaires were used to gather additional information, as needed, based on responses to semi-structured interview questions developed by the researcher to achieve information saturation. Interviews were recorded and transcribed using LiveScribe technology. Qualitative data analysis was conducted through review of interview transcriptions, which led to the development of seven notable themes including: Understanding Military Culture, Understanding Authority, The MSMs Work Roles, Understanding the Unimaginable, Assessing the Whole Person, Assessment Tools and Techniques, and Treating the Whole Person.   Findings indicate there is a general consensus among participants that assessments currently available can be used to address occupational performance of military service members with mild traumatic brain injury; however, the approach used to carry out such assessments must address the uniqueness of military culture in order to achieve the greatest effectiveness and sensitivity.   While it is understood within the profession that occupational therapists should be client-centered and culturally sensitive, the extent to which cultural differences should be attended to may be greater when assessing occupational performance of military service members because of the magnitude of unique cultural aspects. Â
East Carolina University
2013
Master's Thesis
http://hdl.handle.net/10342/4341
https://thescholarship.ecu.edu/bitstream/10342/4341/3/Pippin_ecu_0600M_11091.pdf.txt
52be2385b5c532b6e6427d115f050c6b
https://thescholarship.ecu.edu/bitstream/10342/4341/1/Pippin_ecu_0600M_11091.pdf
37af78d35894b467d111d894ee19ccaf
https://thescholarship.ecu.edu/bitstream/10342/4341/5/Pippin%2c%20Kelly%20Michelle.pdf
e3eb9cb60669d98caa6d817ce77f3e65
Occupational Therapy
Assessment
Mild traumatic brain injuries
Military
Military culture
Occupational performance
Brain Injuries
Military Personnel--psychology
Veterans--psychology
Activities of Daily Living
Qualitative Research
Occupational Therapy--methods
oai:TheScholarship.intra.ecu.edu:10342/68212022-10-03T17:20:10Zcom_10342_73com_10342_1col_10342_117col_10342_72
An Examination of Nursing's Contribution to Global Health
Curtis, Jacqueline
Larson, Kim
Nurses provide the vast majority of front-line global healthcare, but little is known about the contribution that nursing has made to global population health. Study abroad programs have become a popular venue for engaging nursing students in global health initiatives. Yet, many programs lack reliable tools and evaluation methods to measure the contribution of nursing. Global health is one of the four priorities for the future of nursing science (Eckhardt et al., 2017). The purpose of this study was to examine the contribution of nursing to global health.
A qualitative descriptive study was conducted with five nursing students who completed a 2017 study abroad program, in either Guatemala or Nicaragua. In-person interviews were conducted, audio-taped and transcribed. Content analysis was used to look for commonalities and differences within and between cases. Three themes were identified: Seeking Sustainability, Practicing Advocacy, and Engaging in Informed Practice. Seeking Sustainability described efforts by participants to build community partnerships which empowered communities to continue the health promotion efforts. Practicing Advocacy defined how participants used the knowledge and experience they gained to improve care for host country nationals and Latino patients in the US. Engaging in Informed Practice involved applying principles of safety, quality, and ethical behavior to clinical practice abroad. The only outcome-specific program identified by participants was the water filter study in Guatemala. Most participants could not name a measurement or evaluation tool used during their program. This suggests a need for further research, since evaluation of nursing initiatives’ contributions to global health helps fulfill the United Nation 2030 Sustainability Development Goals.
East Carolina University
2018-05-01
Honors Thesis
http://hdl.handle.net/10342/6821
https://thescholarship.ecu.edu/bitstream/10342/6821/1/CURTIS-HONORSTHESIS-2018.pdf
00533c500d61f981b4d1e613748ae636
https://thescholarship.ecu.edu/bitstream/10342/6821/2/LICENSE.txt
960eea43c11ce9eed2255899f1292b0e
https://thescholarship.ecu.edu/bitstream/10342/6821/4/CURTIS-HONORSTHESIS-2018.pdf.txt
7e757e029f338d3bda2f318f26fbaa42
nursing
global health
oai:TheScholarship.intra.ecu.edu:10342/50192021-03-03T20:58:25Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Genetic control of cell fate specification in Caenorhabditis elegans germline.
Mamillapalli, Srivalli Swathi
Lee, Myon-Hee
The precise regulation of germ cell fates (sperm or oocyte) lies at the heart of reproduction and fertility. The nematode Caenorhabditis elegans hermaphrodites produce a discrete number of sperm during larval development and then switch to produce oocyte during adulthood. A number of positive (e.g., fbf genes) and negative (e.g., gld-3) regulators are important for this switch. Here, we found that aberrant activation of MPK-1 (an ERK homolog) by removal of both fbf-1 and lip-1 partially inhibits sperm-oocyte switch, resulting in Mog (masculinization of germline) sterility. The fbf-1 gene encodes a conserved PUF (Pumilio and FBF) RNA-binding protein and the lip-1 gene encodes an MPK-1/ERK phosphatase. Notably, inhibition of MPK-1/ERK signaling by either genetic mutation or chemical inhibition reprograms the germ cell fate and thus helps in regaining the fertility. We also found that fbf-1; lip-1 Mog sterility was enhanced by the depletion of G2/M cell cycle regulators, including CYB-3/Cyclin B, CDK-1/CDK1, and CDC-25.1/CDC25. Markedly, cdc-25.1 mRNA is a direct target of FBF-1. These results suggest that FBF-1 and LIP-1 may promote sperm-oocyte switch by activating MPK-1/ERK signaling and G2/M cell cycle progression.
East Carolina University
2015
Master's Thesis
http://hdl.handle.net/10342/5019
https://thescholarship.ecu.edu/bitstream/10342/5019/1/MAMILLAPALLI_ecu_0600O_11516.pdf
4c7f9de0d3f0b26814c68efec2c7a9a3
https://thescholarship.ecu.edu/bitstream/10342/5019/2/MAMILLAPALLI_ecu_0600O_11516.pdf.txt
facfb804b5ec0a62ed9135611edc5fb0
https://thescholarship.ecu.edu/bitstream/10342/5019/4/MAMILLAPALLI_NEDL.pdf
dda0c66e2cdac942019b6419df1391c9
Developmental biology
Genetics
Medicine
C. elegans
Cell cycle regulation
Cell fate specification
Genetic controls
Germlines
MPK-1/ERK signaling
Caenorhabditis elegans
Spermatogenesis--physiology
Disorders of Sex Development-- metabolism.
Disorders of Sex Development-- genetics
Caenorhabditis elegans--growth & development
Caenorhabditis elegans--metabolism
RNA-Binding Proteins--metabolism
oai:TheScholarship.intra.ecu.edu:10342/91242022-12-14T17:29:51Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
PUF-8 and MPK-1: Genetic and Chemical Control of Spermatocyte Dedifferentiation in Caenorhabditis elegans
Gaddy, Matthew A.
Lee, Myon-Hee
Stem cells face a number of major fate decision during their development: the decision to self-renew or differentiate, and then whether to remain differentiated or dedifferentiate, as occurs in some oncogenesis. A regulatory network controlling these decisions is vital to the development of all multicellular organisms, including humans. Aberrant regulation can result in either loss of specific cell type or uncontrolled cell proliferation, leading to tumors. However, our understanding of how differentiated cell can be reverted to an undifferentiated state remains far more limited.Using the nematode C. elegans germline as a model system, we previously reported that PUF-8 (a PUF RNA-binding protein) and LIP-1 (a dual-specificity phosphatase) inhibit the formation of germline tumors via repressing the dedifferentiation of spermatocytes into mitotic cells (termed "spermatocyte dedifferentiation") at least in part by inhibiting MPK-1 (an ERK MAPK homolog) activation. To gain insight into the molecular competence for spermatocyte dedifferentiation, we compared the germline phenotypes between two competent mutants -- puf-8(q725); lip-1(zh15) with a high MPK-1 activity and puf-8(q725); fem-3(q20gf) with a low MPK-1 activity. puf-8(q725); lip-1(zh15) mutants developed germline tumors more aggressively than puf-8(q725); fem-3(q20gf) mutants at 25°C with aging. This result suggests that MPK-1 activation is critical to induce the formation of germline tumors via spermatocyte dedifferentiation. This idea was confirmed by treatment of puf-8(q725); fem-3(q20gf) mutant worms with Resveratrol, which stimulates MPK-1 activation. Our results show that 100 mM RSV significantly induced the formation of germline tumors via spermatocyte dedifferentiation at 25°C with aging. Therefore, we conclude that MPK-1 activation is required to promote the formation of germline tumors via spermatocyte dedifferentiation in the absence of PUF-8. Since PUF-8 and MPK-1 are broadly conserved, we therefore suggest that similar molecular mechanisms may control dedifferentiation-mediated tumorigenesis in other organisms, including humans.
East Carolina University
2021-05-03
Master's Thesis
en
http://hdl.handle.net/10342/9124
https://thescholarship.ecu.edu/bitstream/10342/9124/1/GADDY-MASTERSTHESIS-2021.pdf
37a1ef4e92a1c4f57d6c4c10934ab272
https://thescholarship.ecu.edu/bitstream/10342/9124/2/LICENSE.txt
1120b03448b5757a40f3f22b5f5e7cba
https://thescholarship.ecu.edu/bitstream/10342/9124/3/PROQUEST_LICENSE.txt
45577290cf638eca545618af68341b1d
https://thescholarship.ecu.edu/bitstream/10342/9124/4/ECUThesisSignaturePage.pdf
c6259a6b1f6f13a66162849b0599c276
https://thescholarship.ecu.edu/bitstream/10342/9124/5/Vireo-NonExclusive-Distribution_License_Edited-Verio-Version_L0615-2.pdf
67ecc0b8fab6c2953d9c894c77929d3a
https://thescholarship.ecu.edu/bitstream/10342/9124/7/GADDY-MASTERSTHESIS-2021.pdf.txt
59b7420af2bbb4b22a66acc6e2a55f7d
Differentiation
Dedifferentiation
Tumorigenesis
MPK-1
PUF-8
Germline
Resveratrol
Spermatocytes
Caenorhabditis elegans
Animals
Caenorhabditis elegans Proteins
oai:TheScholarship.intra.ecu.edu:10342/44072022-11-28T15:38:28Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Use of monoamine oxidase and redox enzymes in atrial tissue as novel predictors of postoperative atrial fibrillation
Darden, Timothy M.
Anderson, Ethan
Postoperative atrial fibrillation (POAF) occurs in approximately 30% of cardiac surgery patients. The complication occurs despite advances in surgical procedures. It is associated with increased mortality, morbidity, and healthcare cost. The exact pathogenesis of this complication is unknown, but oxidative stress and inflammation are considered to be significant factors.   A precisely controlled balance between reactive oxygen species (ROS) generation and ROS scavenging influence the oxidative environment within cells and tissues. Increased activity of monoamine oxidase (MAO) activity, a ROS generating enzyme, can produce a more oxidative environment. A decrease in glutathione (GSH), a ROS scavenging molecule, can also yield an oxidative environment. The enzymes GSH-peroxidase (GPx) and GSH-reductase (GR) are responsible for maintaining the cellular redox environment within a range that is compatible with favorable homeostasis. Despite the well-characterized role that these enzymes play in maintaining redox environment, a comprehensive evaluation of these enzymes in human myocardium has never been attempted.  Furthermore, since reports have recently documented the association between oxidative stress in atrial tissue and the incidence of POAF, we tested the hypothesis that these enzymes are associated with POAF, in a cohort of patients undergoing cardiac surgery at Vidant Medical Center, East Carolina Heart Institute (ECHI).  Human right atrial appendage tissue were obtained from 244 patients undergoing elective coronary artery bypass graft surgery at ECHI between January, 2010, and December, 2012. The generation of ROS were determined using assays on MAO, NADPH oxidase (NOX), along with the activity of glutathione reductase (GR) and glutathione peroxidase (GPx). Patient outcomes, including POAF development, were analyzed in relation to the assays performed. A statistical model was then created to measure the association for risk of POAF development.   This was the first study to determine that MAO activity is a major source of ROS in human atrial tissue. MAO activity is significantly associated with POAF. Total glutathione (GSHt) activity is inversely related to POAF development. GPx is also significantly associated with POAF as well, but the trend is not a linear. GR activity is not correlated with POAF.   MAO, GSHt and GPx are enzymes that contribute to the atrial oxidative environment and increased risk of POAF development. Because this is a pilot study, further exploration is needed to validate our study and to determine if and where these enzymes fit in the etiology of POAF. Â
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4407
https://thescholarship.ecu.edu/bitstream/10342/4407/3/Darden.pdf
dbb780ef5fb2e4c7c3436dbdd616d404
https://thescholarship.ecu.edu/bitstream/10342/4407/1/Darden_ecu_0600O_11170.pdf
648fbf6bfc49b84a803f3a1cff4e313e
https://thescholarship.ecu.edu/bitstream/10342/4407/2/Darden_ecu_0600O_11170.pdf.txt
73b6d305af669a13d6d6e7b62dcaccc4
Health sciences
Pharmacology
Surgery
Cardiopulmonary bypass
Catecholamines
Monoamine oxidase
Oxadative stress
Postoperative atrial fibrillation
Redox
Atrial Fibrillation--metabolism
Atrial Fibrillation--prevention & control
Postoperative Complications--prevention & control
Monoamine Oxidase--metabolism
oai:TheScholarship.intra.ecu.edu:10342/101922022-03-25T07:27:16Zcom_10342_73com_10342_122col_10342_117col_10342_124
The influence of gender on the level of human need fulfillment
Moore, Hope Sylvain
McSweeney, Maryellen
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10192
https://thescholarship.ecu.edu/bitstream/10342/10192/1/Moore_The-influence-of-gender-on-the_1989.pdf
ac5d739972fc7be1b1c4ccb985ac5269
https://thescholarship.ecu.edu/bitstream/10342/10192/2/Moore_The-influence-of-gender-on-the_1989.pdf.txt
809525b835078859c4122e4d1cfe14b6
NURS
oai:TheScholarship.intra.ecu.edu:10342/40642021-03-03T20:57:38Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Impact of preschool handwriting without tears instruction one year following intervention
Phelps, Anna Call
Donica, Denise
Handwriting is a communication skill that must be taught to young students as it serves as the primary manner in which elementary school students are able to demonstrate their knowledge of academic concepts. Occupational therapists who work in the school system commonly engage students who struggle with handwriting skills in specific occupations to reach the goals of improving handwriting abilities and achieving overall academic success. Handwriting Without Tears (HWT)® is a program used by some therapists to teach students the basic component skills of handwriting and proper letter/number formation, sizing, and spacing in order to improve functional written communication.   This paper illustrates the outcomes of a quantitative study that compared results of the Shore Handwriting Screening between students who participated in the HWT Get Set for School program at a Head Start center and those students in control and alternative intervention groups one year following the initial study. Â
East Carolina University
2012
Master's Thesis
http://hdl.handle.net/10342/4064
https://thescholarship.ecu.edu/bitstream/10342/4064/1/Phelps_ecu_0600M_10799.pdf
b2f03c3df19ab677fc291ed1ff943ea8
https://thescholarship.ecu.edu/bitstream/10342/4064/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/4064/3/Phelps_ecu_0600M_10799.pdf.txt
990316464102f0d948f5ddd30ccec5c8
Occupational Therapy
Fine motor skills
Handwriting
Handwriting without tears
Children's skills test
Child, Preschool
Motor Skills--physiology
Psychomotor Performance
Treatment Outcome
Occupational Therapy--methods
oai:TheScholarship.intra.ecu.edu:10342/91352022-12-13T18:17:23Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
The Circadian Rhythm and its Role in the Dynamic Dopamine Neuron Phenotype
Barker, Samantha
Eells, Jeffrey B.
The circadian rhythm is strongly implicated in many neuropsychiatric and neurodegenerative disorders, all of which are associated with altered dopamine (DA) neurotransmission in the substantia nigra and ventral tegmental area. Progress has been made in elucidating the circadian rhythm-dopaminergic network and its role in the onset of neuropsychiatric and neurodegenerative disorders. Previous research suggests that circadian rhythm transcription factors are responsible for directly regulating the DA phenotype; however, it is currently unknown what the relationship between the circadian rhythm and dopaminergic genes looks like with respect to age and time of day. Using a transgenic mouse model with Cre recombinase expression under control of dopamine transporter (DATCre) and yellow fluorescent protein (YFP) Cre reporter and immunohistochemistry techniques, we are able to characterize sub-populations of DA neurons in the ventral midbrain (VMB) that are responsive to the circadian rhythm. Here, we demonstrate a dynamic DA neuron phenotype, where classic dopaminergic markers, such as dopamine transporter (DAT) and tyrosine hydroxylase (TH) are not detected in dopaminergic neurons, due to regulation by the circadian rhythm. In this study, mice transgenic for DATCre/YFP were analyzed at postnatal day 0 (P0), P21, P35 and adulthood (>P60). Each time point included mice taken at subjective dawn (circadian time 0) and subjective dusk (CT12), excluding P0 mice. Results revealed that between P21 and P35, there was a significant loss of the DA neuron phenotype at CT12, as compared to CT0. There was no statistical difference between P35 and adults at CT0 or CT12. This suggests that between P21 and P35, DA neurons begin to transition to a 'former' phenotype throughout the circadian rhythm. Additionally, qRT-PCR data revealed abnormal dopaminergic gene mRNA levels at P21. Elucidating the molecular characteristics of these DA neurons is crucial to understanding the biological mechanisms behind the dopaminergic-circadian rhythm network, which will have future implications in understanding neuropsychiatric and neurodegenerative disorders.
East Carolina University
2021-05-04
Master's Thesis
en
http://hdl.handle.net/10342/9135
https://thescholarship.ecu.edu/bitstream/10342/9135/1/BARKER-MASTERSTHESIS-2021.pdf
54278c4d5bdb037db0848aa336594343
https://thescholarship.ecu.edu/bitstream/10342/9135/2/LICENSE.txt
85fc3b2cd2d595d32892ed112bc5a60f
https://thescholarship.ecu.edu/bitstream/10342/9135/3/PROQUEST_LICENSE.txt
2f0179ce9fd4c0b521f26124654f09a2
https://thescholarship.ecu.edu/bitstream/10342/9135/4/SBarker_Thesis%20Signiture%20Page.pdf
f8b038f509889a08dbfe3ea9840e63b0
https://thescholarship.ecu.edu/bitstream/10342/9135/5/SBarker_Thesis%20NEDL.pdf
16502429b92be1a9926952d0dbc3d73b
https://thescholarship.ecu.edu/bitstream/10342/9135/7/BARKER-MASTERSTHESIS-2021.pdf.txt
c6572d7def3a9a135c667a8185887e63
Neurobiology
Circadian Rhythm
Dopaminergic Neurons
Phenotype
Retina
oai:TheScholarship.intra.ecu.edu:10342/46372021-03-03T20:56:28Zcom_10342_122com_10342_2com_10342_1com_10342_107com_10342_73col_10342_124col_10342_8858col_10342_112
The effectiveness of the Interactive Metronome® as a tool to improve selective attention of veterans within their roles in post-secondary education settings in eastern North Carolina
Baker, Karla
Trujillo, Leonard
The purpose of this study was to measure the effectiveness of the Interactive Metronome specific to improving attention. As veteran enrollment continues to increase in higher education, attention in the classroom and workforce due factors such as Post Traumatic Stress Disorder (PTSD) and Traumatic Brain Injury (TBI) have become an issue. Could veterans who have expressed concern of attention problems benefit from the Interactive Metronome (IM)? Three veteran students and employees from East Carolina University self-identified as having problems with attention and participated in the study. Subjects were given the IM-Home system after meeting with the PI to complete the Canadian Occupational Performance Measure (COPM), d2 Test of Attention, and learning to use the system. After 15 at-home sessions (4-6 weeks) with the IM, participants retook the COPM and D2. It was found that all participants rated their satisfaction with attention in school or work higher than before they started the IM. Additionally, post-test scores of the d2 Test of Attention showed that all participants were able to process more information. Based on these results and past studies, it is believed that the Interactive Metronome is a valuable tool in the rehabilitation process and may be especially helpful for veterans with attention problems.
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4637
https://thescholarship.ecu.edu/bitstream/10342/4637/1/Baker_ecu_0600O_11341.pdf
eacac55be1276b6097ca30d10ba82bd0
https://thescholarship.ecu.edu/bitstream/10342/4637/2/Baker_NEDL.pdf
45952f11c1e6fe8ed1fff6ddb5809ae1
https://thescholarship.ecu.edu/bitstream/10342/4637/3/Baker_ecu_0600O_11341.pdf.txt
5ebfd5f7f4d7241399a1028928ad1974
Occupational Therapy
East Carolina University--Employees
Stress Disorders, Post-Traumatic--physiopathology
Mental Processes--physiology
Attention--physiology
Veterans--education
Behavior Therapy--instrumentation
Behavior Therapy--methods
oai:TheScholarship.intra.ecu.edu:10342/72952022-09-30T12:17:44Zcom_10342_74com_10342_73com_10342_1col_10342_75col_10342_72
TIMECOURSE OF SYNAPSE DEVELOPMENT IN HUMAN IPSC-DERIVED NEURONS
Orbita, Adrienne Marie
Litwa, Karen
Understanding the underlying mechanisms in brain formation give an insight to what affects the formation of the brain when affected with autism, specifically, the excitatory and inhibitory synapses. The mechanism of how it is regulated in the brain is important because it shows the inherent development of the brain when affected with a neurodegenerative disease. The studying of these synapses also give light to any disruption of function in the brains affected with autism. Autism is associated with affecting the regions of the brain that control social interaction, communication, and their reasoning. As autism prevalence rises, there is a greater need to understand these mechanisms to boost the knowledge than can be used for further research.
To make this a feasible task, human induced pluripotent stem cells (hIPSC) are used to conduct these experiments. This allows for a model that can develop both excitatory and inhibitory synapses that can recapitulate synapse development and gather insight on the 3-D and 2-D models created. In this experiment, the motion of decreased inhibitory synapses will result in an increase of excitatory synapse formation in 3-D models. In 2-D models, synapse area will increase with the increase in neurite length and density over time. The result from these various experiments contribute to a greater understanding to the mechanisms of these synapses and show the relationship of how important these are to the development of the human brain. The project also supports the future directions of using data from this experiment to observe drug studies that may include ROCK inhibitors and previous research to be able to observe further development of the stem cells.
East Carolina University
2019-06-12
Honors Thesis
http://hdl.handle.net/10342/7295
https://thescholarship.ecu.edu/bitstream/10342/7295/1/ORBITA-HONORSTHESIS-2018.pdf
1f3e90efce94e09681a061a4cca98420
https://thescholarship.ecu.edu/bitstream/10342/7295/2/LICENSE.txt
0c970009d09e7cd823ee2e2b20e25e2a
https://thescholarship.ecu.edu/bitstream/10342/7295/4/ORBITA-HONORSTHESIS-2018.pdf.txt
f9f23ba5df36281bf56eb889595e100f
autism, hIPSC, synapses
oai:TheScholarship.intra.ecu.edu:10342/101642022-03-25T07:25:14Zcom_10342_73com_10342_122col_10342_117col_10342_124
A descriptive study of telephone communications between diabetes clients and diabetes nurse educators
Leggett-Frazier, Nancy
Pokorny, Marie E.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10164
https://thescholarship.ecu.edu/bitstream/10342/10164/1/Leggett-Frazier_A-descriptive-study-of-telephone-communications_1994.pdf
1f4ef14cddb4a485af2119883b9b280e
https://thescholarship.ecu.edu/bitstream/10342/10164/2/Leggett-Frazier_A-descriptive-study-of-telephone-communications_1994.pdf.txt
b2861c9752712817c160c2da95de9416
NURS
oai:TheScholarship.intra.ecu.edu:10342/104342023-10-19T17:02:15Zcom_10342_73com_10342_122col_10342_117col_10342_124
The correlation between life satisfaction and physical functioning of spinal cord injured individuals following discharge from rehabilitation
Dunnum, Linda R.
Vincent, Pauline
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10434
https://thescholarship.ecu.edu/bitstream/10342/10434/1/Dunnum_The-correlation-between-life-satisfaction-and_1987.pdf
b34eabd7a7c0da49a5d392ddab5c042b
https://thescholarship.ecu.edu/bitstream/10342/10434/2/Dunnum_The-correlation-between-life-satisfaction-and_1987.pdf.txt
6c5a147b4992faf5a1a049292bbff709
https://thescholarship.ecu.edu/bitstream/10342/10434/4/Hofler_Permissions_Yes.pdf
6e0acaf70c4f5ad2a4dd0e7947b53d91
NURS
oai:TheScholarship.intra.ecu.edu:10342/31702021-03-03T20:52:42Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Influence of linguistic context and working memory on auditory comprehension in young and older adults with aphasia
Yu, Kun
Hough, Monica Strauss
The purpose of the current investigation was to examine the influence of linguistic context on auditory comprehension in adults with aphasia, explore effects of the explanatory variables of age, working memory (WM), aphasia severity, and auditory comprehension relative to linguistic contextual influences, and investigate relationships among these explanatory variables.  Eight young (<60) and eight older (>60) individuals with aphasia as the result of a left hemisphere cerebrovascular accident (CVA) participated in the investigation. The participants underwent pre-experimental testing, including two subtests of the Boston Diagnostic Aphasia Examination-III to confirm presence of auditory comprehension impairment as well as ability to perform the experimental tasks. The Western Aphasia Battery-Revised also was administered to determine the presence and severity of aphasia. The participants were administered a series of experimental tasks, including listening span to measure WM capacity, modified Token Test to measure auditory comprehension, and a linguistic context task to examine the influence of predictive and non-predictive contexts on auditory comprehension of passive and active sentences.  Results indicated that age did not appear to influence WM, aphasia severity, and auditory comprehension skills in this group of aphasic individuals. Thus, the persons with aphasia (PWA) had reduced WM capacity, regardless of age. However, decreased severity of aphasia was highly related to both increased WM capacity and auditory comprehension skills; that is, WM capacity as well as auditory comprehension increased as severity of aphasia decreased. Moreover, a strong relationship was observed between WM and auditory comprehension, indicating that auditory comprehension increased with increasing WM.   Non-predictive context facilitated comprehension of active sentences more than predictive context. Predictive context may have had an adverse influence on comprehension of active sentences, as the PWA may have "lost interest" as well as experienced decreased attention when they heard target sentences containing "old" information that was consistent and possibly repetitious of preceding linguistic context. Non-predictive context facilitated comprehension of active sentences because participants were presented with novel information that was not conveyed in target sentences. However, predictive context was more beneficial than non-predictive context in the comprehension of passive sentences. The PWA had difficulty with passive sentences possibly due to syntactic complexity and semantic reversibility of the sentence contexts. Predictive context facilitated comprehension of the passive sentences because it provided semantic constraints and made one interpretation of target sentences more plausible than the other. In contrast, the non-predictive context simply familiarized the participants with the lexical items of passive sentences; it did not provide the semantically supportive framework, thus making it more difficult to determine which interpretation of the passive sentences was more plausible. This latter result is a robust finding that is consistent with previous research and continues to require further exploration relative to its use in language treatment in aphasia. Â
East Carolina University
2010
Master's Thesis
http://hdl.handle.net/10342/3170
https://thescholarship.ecu.edu/bitstream/10342/3170/2/Yu_ecu_0600M_10285.pdf.txt
281d52d630fdc750219b7f5d71f6cacc
https://thescholarship.ecu.edu/bitstream/10342/3170/1/Yu_ecu_0600M_10285.pdf
4312633da053af4570a9ce6f4bce7dd7
https://thescholarship.ecu.edu/bitstream/10342/3170/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Speech Therapy
Working Memory
Aphasia
Cerebrovascular Accident
Language Disorders
Auditory Perceptual Disorders
Language Therapy
Neuropsychological Tests
Speech Disorders
Rehabilitation
oai:TheScholarship.intra.ecu.edu:10342/42472021-03-03T20:56:06Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Using TIRF microscopy to analyze stimulated and basal state B-cell MHC II clustering in response to ageing and dietary fish oil
Melton, Mark T.
Shaikh, Saame Raza
This research focused on developing an efficient TIRF microscopy approach to evaluate membrane protein organization. More specifically, the data demonstrate that TIRF microscopy can detect changes in ex vivo B-cell MHC II lateral organization using a monoclonal antibody under differing conditions. MHC II clustering is dependent on the underlying lipid environment and upon LPS stimulation MHC II expression is dramatically increased. Using mice fed a fish oil or control diet for three weeks, or using mice aged for nine months, we imaged splenic B-cell MHC II clustering using TIRF microscopy. We also used LPS to stimulate B-cells from both experimental conditions to determine if either ageing the animals or feeding them fish oil could affect MHC II clustering. We then determined cluster quantity, size, and intensity using the NIH ImageJ software. The data showed that neither a relevant dose of fish oil, nor aging the mice approximately nine months, had an affect on MHC II clustering in the absence of LPS stimulation. However upon LPS stimulation, MHC II clustering dramatically changed in aged mice as well as fish oil fed mice compared to control animals. Taken together, the data establish the TIRF microscopy protocol as a relevant alternative to more costly and time consuming approaches to address membrane protein clustering. Moreover, either ageing the animals or feeding them fish oil significantly affects MHC II clustering upon stimulation with LPS. Â
East Carolina University
2013
Master's Thesis
http://hdl.handle.net/10342/4247
https://thescholarship.ecu.edu/bitstream/10342/4247/2/melton.pdf
08e18500464278dacfe879da19948497
https://thescholarship.ecu.edu/bitstream/10342/4247/3/Melton_ecu_0600M_11039.pdf.txt
86d3418edcb737c7124828145befcf56
https://thescholarship.ecu.edu/bitstream/10342/4247/1/Melton_ecu_0600M_11039.pdf
491493ec120e67d569ff137b26e6014c
Chemistry, Biochemistry
Immunology
B cells
Lipid rafts
MHC class II
Plasma membrane
Protein clustering
TIRF
Biochemistry
Microscopy--methods
Membrane Microdomains
Cell Membrane Structures
Biochemical Phenomena
oai:TheScholarship.intra.ecu.edu:10342/102662023-01-04T16:50:41Zcom_10342_73com_10342_122col_10342_117col_10342_124
A comparison of nurse and patient perceptions of patient's postsurgical pain
Stephenson, Nancy Lou Nicks
Brown, Sylvia T.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10266
https://thescholarship.ecu.edu/bitstream/10342/10266/1/Stephenson_A-comparison-of-nurse-and-patient_1990.pdf
266b4db0195caf8e8a72e417be1250e6
https://thescholarship.ecu.edu/bitstream/10342/10266/2/Stephenson_A-comparison-of-nurse-and-patient_1990.pdf.txt
527581a135b5e47ead0202f326428e6f
https://thescholarship.ecu.edu/bitstream/10342/10266/4/Stephenson_Permissions_Yes.pdf
bbe231e24db9ad61b3579f15643c85c3
NURS
oai:TheScholarship.intra.ecu.edu:10342/68292022-09-30T17:28:04Zcom_10342_73com_10342_1col_10342_117col_10342_72
Exploring the Use of Integrative Therapies during Pregnancy
Nelson, Taylor
Reis, Pamela
Background: About 60% of women experience back pain while pregnant (Jayson, 2016). “Morning sickness� affects about 80-90% of pregnant women (Viljoen, Visser, Koen, & Musekiwa, 2014). Stress and anxiety during pregnancy can cause negative birth outcomes (Effati-Daryani, Mohammad-Alizadeh-Charandabi, Mirghafourvand, Taghizadeh, & Mohammadi, 2015). Purpose: The purpose of this research was to (1) explore the published literature regarding the use of integrative therapies during pregnancy for low back and pelvic pain (LBPP), nausea and vomiting (N/V), and depressed mood or anxiety, and (2) to survey ethnic and racially diverse pregnant women receiving prenatal care at a birth center in North Carolina about their use of integrative therapies to alleviate common discomforts of pregnancy, including LBPP, N/V, and depressed mood or anxiety. Methodology: A convenience sample of 31 was obtained by requesting participation in a survey from pregnant women at a birth center in Chapel Hill, North Carolina that assessed their use of integrative therapies during pregnancy. Pregnant women of all trimesters and races were included in the study. Those women who were unable to speak and read English were excluded from this study. Results: Yoga (32.3%) and chiropractic services (29.1%) were commonly reported integrative therapies used to treat low back and pelvic pain during pregnancy. Acupressure (12.9%) was the most commonly reported integrative therapy used to treat nausea and vomiting during pregnancy. Meditation/yoga (6.5%) and herbs (6.5%) were commonly reported integrative therapies used to treat mood disturbances during pregnancy. About 23% of participants reported having used integrative therapies during pregnancy without notifying their healthcare providers. Implications: The findings of this research were used to understand what integrative therapies women are using to alleviate their common discomforts of pregnancy, specifically related to LBPP, N/V, & mood disturbances.
East Carolina University
2018-05-03
Honors Thesis
http://hdl.handle.net/10342/6829
https://thescholarship.ecu.edu/bitstream/10342/6829/1/NELSON-HONORSTHESIS-2018.pdf
5d7f79e7b94963ff6d60730910f05959
https://thescholarship.ecu.edu/bitstream/10342/6829/2/LICENSE.txt
34c853222498d1b5c0a730966ea2cab9
https://thescholarship.ecu.edu/bitstream/10342/6829/4/NELSON-HONORSTHESIS-2018.pdf.txt
ad1527670d0b24b68dd31c61b79271f9
pregnancy
pregnant
integrative therapies
CAM
alternative therapies
discomforts
oai:TheScholarship.intra.ecu.edu:10342/101182022-03-25T07:16:01Zcom_10342_73com_10342_122col_10342_117col_10342_124
Neonatal nurses selection of appropriate intervention in response to preterm infant behaviors
Sarvey, Sharon Isenhour
Henning, Emilie D.
East Carolina College
Thesis
en
http://hdl.handle.net/10342/10118
https://thescholarship.ecu.edu/bitstream/10342/10118/1/Sarvey_Neonatal-nurses-selection-of-appropriate-intervention_1988.pdf
97fe1534fe6bffa0096c09e0ce067509
https://thescholarship.ecu.edu/bitstream/10342/10118/2/Sarvey_Neonatal-nurses-selection-of-appropriate-intervention_1988.pdf.txt
19b2f3b67210e0f975feb7636a4f6ec4
NURS
oai:TheScholarship.intra.ecu.edu:10342/104772022-03-29T07:21:27Zcom_10342_73com_10342_122col_10342_117col_10342_124
Nursing director management style and staff nurse job satisfaction in local public health departments
Hogan, Karen C.
Lawler, Therese G.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10477
https://thescholarship.ecu.edu/bitstream/10342/10477/1/Hogan_Nursing-director-management-style-and-staff_1988.pdf
dc82ef3ef7ca5ab22b4482caa0831759
https://thescholarship.ecu.edu/bitstream/10342/10477/2/Hogan_Nursing-director-management-style-and-staff_1988.pdf.txt
f97b8a304bd23e96b865e5edf29f42c2
NURS
oai:TheScholarship.intra.ecu.edu:10342/74732022-12-12T18:51:05Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
30-Day Immunotoxicity Study of PFMOAA in C57BL/6 Mice
Vance, Samuel
DeWitt, Jamie C.
Within the past five years, two classes of per- and polyfluoroalkyl substances (PFAS) were phased out of production in the U.S., which led to the development and production of PFAS to replace these two major classes. One family of these PFAS are perfluoro-ether carboxylic acids (PFECA), which have emerged in the public and scientific arenas due to their presence in drinking water systems across the U.S., including Wilmington, NC. Although manufacturers have touted them as having more favorable environmental and toxicological properties very little is known about the toxicity and environmental fate these emerging PFECA. One compound, perfluoro-2-methoxyacetic acid (PFMOAA), was identified as the dominant PFECA in the Cape Fear River, in concentrations as high as 35,000 ng/L. There is very little mention of PFMOAA in the publicly available scientific literature and to our knowledge, we are the first to investigate its potential for toxic effects. In this 30-day study, we orally administered 25,000, 2,500,000, or 250,000,000 ng/L of PFMOAA in water to male and female C57BL/6 mice and investigated immune and liver alterations following exposure. Mice given PFMOAA showed no signs of overt toxicity during the study and no evident changes were observed in liver mass or peroxisomal enzyme activity. While mild alterations in splenic and thymic lymphocyte sub-populations were observed in males, these results do not point to any definitive alterations in immune function. Ultimately, we concluded that the doses administered were too low to achieve an internal dose sufficient to induce changes to immune endpoints, likely due to rapid excretion of PFMOAA in mice. Further investigation into serum and organ concentrations of PFMOAA as well its effects on antibody production will be more conclusive of immunotoxic effects.
East Carolina University
2019-07-18
Master's Thesis
en
http://hdl.handle.net/10342/7473
https://thescholarship.ecu.edu/bitstream/10342/7473/1/VANCE-MASTERSTHESIS-2019.pdf
48265cfcd282c16dbf4896f0f97d173d
https://thescholarship.ecu.edu/bitstream/10342/7473/2/LICENSE.txt
9a65d290b845fc4c88d46d008f169922
https://thescholarship.ecu.edu/bitstream/10342/7473/3/PROQUEST_LICENSE.txt
4fd0c626e20c595311bb19755219619a
https://thescholarship.ecu.edu/bitstream/10342/7473/4/SUNCW20519071110490.pdf
e8b9348dd7c1724d36e1a1cfa56c7cb4
https://thescholarship.ecu.edu/bitstream/10342/7473/5/liscensepfmoaa.pdf
5b83118da5ec66d3b84e7e64469b0f67
https://thescholarship.ecu.edu/bitstream/10342/7473/7/VANCE-MASTERSTHESIS-2019.pdf.txt
1359fb786cf580a7a7b8763a88aa3131
PFAS
PFMOAA
PFOA
Environmental Pollutants
Mice
Mice, Inbred C57BL
oai:TheScholarship.intra.ecu.edu:10342/44642021-03-03T20:56:43Zcom_10342_73com_10342_1col_10342_117col_10342_72
The Effects of School Sports Programs on Obesity in Adolescents
Little, Cameron B.
Kim Larson
In the Southeastern United States, healthcare issues related to obesity in adolescents ages 13-18 years have become a growing concern. Along with an increasing body mass index in these adolescents comes many other comorbidities that cost patients and taxpayers millions of dollars annually in healthcare. North Carolina has identified this as a problem and is taking steps to address this issue, including increasing physical activity and addressing nutrition in schools (Park, 2007). Research from the CDC shows that almost one third of North Carolina's population is classified as obese. This means that they have a body mass index of thirty or higher. This is one of the highest percentages in the country. This is a growing concern because the CDC has shown that this trend has been increasing steadily (CDC, 2010). The rural areas in which many of these adolescents live and the access to physical activity in these areas play a major role in this epidemic (Lipman, Schucker, Ratcliffe, Holmberg, Baier, & Deatrick, 2011). Programs such as after-school sports teams can help to provide these adolescents with the access to physical activity that can help combat many of these risk factors of these comorbidities, such as increasing body fat due to sedentary activity. For this to be effective, schools must provide activities that interest the students and will provide them with healthy habits to carry on later in life.
2014
Undergraduate Thesis
http://hdl.handle.net/10342/4464
https://thescholarship.ecu.edu/bitstream/10342/4464/2/Little_Cameron.pdf
d95b60286bb20ab31b5a668e384a8136
https://thescholarship.ecu.edu/bitstream/10342/4464/3/Little_Cameron.pdf.txt
b0ec0e80994ef63c74ecae035a2e4571
Nursing
Obesity
Adolescents
BMI
Wayne County (N.C.)
Sports
oai:TheScholarship.intra.ecu.edu:10342/102602023-01-10T14:47:47Zcom_10342_73com_10342_122col_10342_117col_10342_124
Assessment of the predictive validity of the North Carolina Preterm Labor/Low Birth Weight Risk Assessment form
Blackburn, Sandra G.
Lieber, Marilyn Therese
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10260
https://thescholarship.ecu.edu/bitstream/10342/10260/1/Blackburn_Assessment-of-the-predictive-validity-of_1995.pdf
0ccbaed3cdfd39a4db27d71851c8cd6b
https://thescholarship.ecu.edu/bitstream/10342/10260/2/Blackburn_Assessment-of-the-predictive-validity-of_1995.pdf.txt
b0d282f41431b36d7aee0166d4db8283
https://thescholarship.ecu.edu/bitstream/10342/10260/4/Blackburn_Permissions_Yes.pdf
6d1a71891b6c0d9d9eedc5d677def2bb
NURS
oai:TheScholarship.intra.ecu.edu:10342/69192021-03-03T21:18:44Zcom_10342_122com_10342_2com_10342_1com_10342_107com_10342_73col_10342_124col_10342_8858col_10342_112
The Effectiveness of Visual Scanning Training to Improve Functional Performance Poststroke: A Case Study in Eastern North Carolina
Gartz, Rachel
Dickerson, Anne Elizabeth
Rationale: Although an abundance of research exists regarding overall rehabilitation interventions poststroke, there is a lack of evidence for treatment of visual deficits. Additionally, eye tracking glasses may be used to further understand the effects of visual field deficits poststroke, though no studies have yet used eye tracking in the context of daily occupations. Purpose: This study evaluated the effectiveness of component-based, occupation-based, and combined occupational therapy treatment for visual scanning training on improving occupational performance in instrumental activities of daily living. The second portion of the study described the differences in visual scanning tendencies during cooking and driving, between an individual with a visual field deficit poststroke and a healthy control of similar age and gender. Design: This study used a case study design with a health control for the eye tracking portion. Participant: The participant was a 55-year-old female who had a stroke 7 years prior. Methods: One participant with a visual field deficit poststroke underwent three visual scanning treatments - component-based, occupation-based, and combined. The researchers administered the Assessment of Motor and Process Skills (AMPS) and took standardized measures on the Vision Coach (full field, 60 dots, all red, speed 0, fixator off) at pretest, after the component-based intervention, after the occupation-based intervention, and after the combined intervention to determine the change in occupational performance - measured by motor skills and process skills - after each intervention. After the interventions were complete, the participant completed a cooking tasks and a task on the driving simulator, while wearing the Tobii Glasses Pro 2 eye tracking glasses. Analysis: Data from the AMPS was compared between times and to the AMPS standardization sample to determine observable improvements. Vision Coach data was also compared between times. The participant's eye tracking data - duration of first fixation, total visit duration, and heat maps - were compared to a healthy control of the same age and gender. Results: With regards to the AMPS and in order of time, the participant scores of motor skills were 1.4 (mild to moderate increased physical effort), 1.8 (questionable to mild increased physical effort), 2.0 (questionable increased physical effort), and 1.8 (questionable to mild increased physical effort). Her scores of process skills were 0.8 (questionable to mild inefficiency), 1.0 (questionable inefficiency), 0.8 (questionable to mild inefficiency), 1.2 (questionable inefficiency). As per the AMPS standardization sample, an observable difference is one of 0.30 logits or more. Comparison of the eye tracking measures and heat maps revealed differences between time spent viewing areas of the visual field, including during two crashes on the driving simulator. Discussion: Overall, visual scanning training as a compensatory method was effective for this participant and could therefore be considered by occupational therapists when treating clients with chronic visual field deficits poststroke, with the combined training being most effective. This study also supports the use of eye tracking glasses during occupations to understand visual scanning tendencies between individuals with and without visual deficits.
East Carolina University
2018-06-18
Master's Thesis
en
http://hdl.handle.net/10342/6919
https://thescholarship.ecu.edu/bitstream/10342/6919/1/GARTZ-MASTERSTHESIS-2018.pdf
c43baa5032bae3636106d5e5fb36237f
https://thescholarship.ecu.edu/bitstream/10342/6919/2/LICENSE.txt
838eab8487088fb8801639183ffd0517
https://thescholarship.ecu.edu/bitstream/10342/6919/3/PROQUEST_LICENSE.txt
ba7f5d46a2669653ab6f9b91d184ac54
https://thescholarship.ecu.edu/bitstream/10342/6919/4/Signature%20Pages.pdf
0cb20a2ac35a54acf82eda9bece4c106
https://thescholarship.ecu.edu/bitstream/10342/6919/5/Non-Exclusive-Distribution-License%20AD%20signed.pdf
ab5eacae06248f8c224e4ed015493ee3
https://thescholarship.ecu.edu/bitstream/10342/6919/7/GARTZ-MASTERSTHESIS-2018.pdf.txt
64673d12dd27ecf65ecc971521fb38ce
visual rehabilitation
occupational therapy
preparatory
occupation based
Stroke Rehabilitation
Radionuclide Imaging
Vision, Low
North Carolina
Blindness
oai:TheScholarship.intra.ecu.edu:10342/63932021-03-03T21:15:19Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Structural and functional analysis of the Vaccinia virus O1 virulence protein
Weeks, Anastasia C.
Mannie, Mark D.
Poxviruses are double-stranded DNA viruses capable of causing disfiguring and deadly disease in a wide range of hosts, from insects to mammals. Orthopoxviruses (OPXV) encode many proteins that are not essential for viral replication, but are responsible for vast differences in pathogenesis. Of the>200 proteins in the prototypical OPXV vaccinia virus (VACV), many remain functionally cryptic. The objective of these studies was to understand how the VACV O1 protein functions by investigating cell-specific effects that may contribute to virulence. The O1L gene is expressed early as the O1 protein, a 78 kDa protein that lacked N-linked glycosylation. These data are the first to demonstrate the reduced ability of an O1 deletion mutant ([delta]O1) to induce cell migration compared to the parental VACV Western Reserve strain (VACV-WR). [delta]O1-infected cell monolayers also exhibited reduced plaque diameter and clearance in plaque foci. These observations indicated that O1 is a significant contributor to VACV cytopathic effects (CPE) in vitro, in agreement with published reports. The results reported herein are the first to describe an altered immunological response with [delta]O1, as levels of anti-VACV immunoglobulin significantly increased with [delta]O1 infection at a time point (seven days post-infection) when VACV-WR induced VACV-specific antibody levels were comparable to sera from mock-infected mice. [delta]O1 was more immunogenic in an ex vivo antigen presentation assay, although mitogen-induced CD4+ T cell activation during [delta]O1 infection was equivalent to VACV-WR infection. Surprisingly, of all the immune cell types tested, [delta]O1 significantly differed from VACV-WR infection in the metabolic readout of only one cell type - RAW 264.7 macrophages. VACV-WR infected RAW 264.7 macrophages were more metabolically active than [delta]O1-infected cells at higher infectious doses, which may be indicative of a specialized niche for O1 function. Taken together, these data may provide clues into the mechanism of O1 virulence.
East Carolina University
2017-07-26
Master's Thesis
en
http://hdl.handle.net/10342/6393
https://thescholarship.ecu.edu/bitstream/10342/6393/1/WEEKS-MASTERSTHESIS-2017.pdf
2294fd7086527ad59f04b30f612a354a
https://thescholarship.ecu.edu/bitstream/10342/6393/2/LICENSE.txt
2abd76b8122cb1b45f21ad6c1221026a
https://thescholarship.ecu.edu/bitstream/10342/6393/3/PROQUEST_LICENSE.txt
8f0723c351fa5676b1af12c6910dc586
https://thescholarship.ecu.edu/bitstream/10342/6393/4/Anastasia%20Weeks066.pdf
43655ae444b84468f91828352a494720
https://thescholarship.ecu.edu/bitstream/10342/6393/5/AWeeks%20Nonexclusive%20License.pdf
0e7e1cac495e24708cf42bf6e4429203
https://thescholarship.ecu.edu/bitstream/10342/6393/6/AWeeks%20Nonexclusive%20License_title%20edit.pdf
3b8ab517f5f21616ac465fcd6e745f1b
https://thescholarship.ecu.edu/bitstream/10342/6393/7/AWeeks%20Nonexclusive%20License_title%20edit_1.pdf
3b8ab517f5f21616ac465fcd6e745f1b
https://thescholarship.ecu.edu/bitstream/10342/6393/9/WEEKS-MASTERSTHESIS-2017.pdf.txt
e74b7313d6e20e1258993ce78de49419
poxvirus
O1L
mutant
immune
attenuated
plaque
morphology
cell
migration
Virulence
Viral Proteins
Vaccinia virus
oai:TheScholarship.intra.ecu.edu:10342/43282023-05-31T13:03:32Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
The contribution of motility and chemotaxis in the Borrelia burgdorferi infectious life cycle
Yerke, Aaron
Motaleb, M. D.
Lyme disease has emerged as an increasing problem for people in the east and northeastern part of the United States. It can cause a chronic debilitating infection if left untreated and is difficult to diagnose. The illness is caused by an infection with the spirochete known as Borrelia burgdorferi. B. burgdorferi is a Gram-negative bacterium that is transmitted by ticks of the Ixodes genus. The primary carriers in regions of high Lyme disease incidence are Ixodes scapularis vector and white-footed Mus musculus rodents. B. burgdorferi is not known to produce common virulence factors such as toxins or capsules. Chemotaxis and motility are important for B. burgdorferi to cause infection and are considered as invasive attributes of this organism. Only a handful of studies have reported that non-chemotactic and non-motile B. burgdorferi mutants are unable to disseminate in hosts, and are, therefore, non-infectious in mice. Although motility and chemotaxis has been shown to be crucial for the infectious life cycle of B. burgdorferi, little is known about the mechanism of motility or assembly of periplasmic flagella. It is not known how incremental reductions of motility affect B. burgdorferi's virulence. Recent cryo-electron microscopy tomography revealed that spirochetes possess a unique flagellar structure called the collar. However, the gene or genes that encode for the B. burgdorferi collar proteins are unknown. Because of its location in the periplasmic flagellar motor, we hypothesize that this organelle is important for flagellar assembly as well as motility. We also hypothesize that less motile or less chemotactic mutants will exhibit a reduced invasive phenotype in vivo. Accordingly, using various comprehensive approaches, we have identified several putative genes in the B. burgdorferi genome with no significant similarity to other bacterial species. In vitro gene mutant analyses indicate that the cells are motility deficient. Additionally, fliZ putatively encodes a regulator of the flagella assembly complex. In other bacteria, inactivation of fliZ creates a reduced motile phenotype. To demonstrate if reduced motility is important for survivability or transmission between hosts, we plan to assay these mutants in mouse-tick-mouse infection cycle experiments. Importantly, mutants deficient in chemotaxis response regulator cheY2 exhibit normal motility and chemotaxis in vitro but exhibit reduced virulence in mice. Specifically, the cheY2 mutants were significantly attenuated in mouse infection and dissemination to distant tissues after needle inoculation. Additionally, while ΔcheY2 mutant cells can survive normally in the Ixodes ticks, mice fed upon by the ΔcheY2-infected ticks failed to establish persistent infection. These data suggest that CheY2, despite resembling a typical chemotaxis response regulator, functions distinctively from most other CheY proteins. These data lead us to propose that CheY2 serves as a regulator for a virulence determinant that is required for productive infection within murine, but not Ixodes tick hosts.
East Carolina University
2013
Master's Thesis
http://hdl.handle.net/10342/4328
https://thescholarship.ecu.edu/bitstream/10342/4328/3/Yerke_ecu_0600M_11053.pdf.txt
51056399e9bd13af5607a69a7431c938
https://thescholarship.ecu.edu/bitstream/10342/4328/1/Yerke_ecu_0600M_11053.pdf
27a9c98cda552c81aca9e161590f8217
https://thescholarship.ecu.edu/bitstream/10342/4328/5/Yerke%2c%20Aaron%20Matthew.pdf
e712259a829aeebf4a41ca087a29e17c
https://thescholarship.ecu.edu/bitstream/10342/4328/7/Re_%20Available%20Thesis.pdf
cf74516983c5f96130be469309eab3c1
2024-05-16
Biology, Molecular
Borrelia
Burgdorferi
Chemotaxis
Collar
Flagella
Motility
Molecular biology
Lyme Disease
Ticks
Ixodes
Tick-Borne Diseases
Erythema Chronicum Migrans
Borrelia burgdorferi--pathogenicity
Chemotaxis
Mice
Treponema pallidum
Gram-Negative Anaerobic Bacteria
Gram-Negative Bacterial Infections
Borrelia Infections
oai:TheScholarship.intra.ecu.edu:10342/103542022-04-05T14:06:30Zcom_10342_73com_10342_122col_10342_117col_10342_124
Gerontological course unit and a nursing home experience : the effects on knowledge and attitudes of associate degree nursing students
Davies, Carole Ann
Reynolds, Betty J.
East Carolina University
Master's Thesis
en
http://hdl.handle.net/10342/10354
https://thescholarship.ecu.edu/bitstream/10342/10354/1/Davies_Gerontological-course-unit-and-a-nursing_1989.pdf
65a63c32add057d992059a1c362981db
https://thescholarship.ecu.edu/bitstream/10342/10354/2/Davies_Gerontological-course-unit-and-a-nursing_1989.pdf.txt
76aa6179c0ec834ede367ee2aa2aa000
NURS
oai:TheScholarship.intra.ecu.edu:10342/46632022-12-09T16:06:56Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Fesselin, an intrinsically disordered smooth muscle protein, organizes and stabilizes actin-myosin and myosin
Kingsbury, Nathaniel
Chalovich, Joseph
Fesselin is an intrinsically disordered protein that is known to bind a large variety of cytoskeletal proteins. The proteins fesselin is known to bind include: actin (Leinweber et al. 1999), [alpha]-actinin (Pham et al. 2006), calmodulin (Schroeter et al. 2004), filamin (Weins et al. 2001), and smooth myosin (Schroeter et al. 2005). The binding of fessilin to smooth myosin is of particular interest because unphosphorylated smooth muscle myosin filaments are unstable in the presence of ATP (Trybus et al. 1982, Ikebe et al. 1983, Suzuki et al. 1978). However, in smooth muscle cells unphosphorylated myosin filaments are maintained (Milton et al. 2011). Several proteins have been identified that stabilize myosin filaments and actin-myosin filament interactions. Our experiments show that fesselin may be one such protein. The organization of F-actin and myosin filaments by fesselin was observed by monitoring the rate of dissociation of actin-myosin by ATP in a stopped-flow device. Actin-myosin dissociation was measured by light scattering (a measure of particle size) and by pyrene-actin or acrylodan-tropomyosin fluorescence (a measure of myosin-actin bond breaking). The stopped-flow studies were further supported with electron microscopy analysis. These experiments showed that fesselin was able to tether actin and myosin filaments together without significantly impacting the rate of the actin-myosin bond breaking. The stabilization of myosin filaments by fesselin was tested using a similar method. First, stopped-flow rapid kinetics were used to measure the rate of ATP induced myosin filament break down. Next, electron microscopy was used to support the stopped-flow data and observe the effects of fesselin on myosin filament organization. Through the stopped-flow and electron microscopy experiments it was found that fesselin stabilizes myosin filaments. The electron microscopy experiments further revealed that fesselin enhanced myosin filament size and organized them into bundles. Previously published results showed that the interaction between fesselin and actin is regulated by calmodulin (Schroeter et al. 2004). The final set of experiments presented here examined the possibility that calmodulin regulates the interactions between fessilin and myosin. The calmodulin regulation of fesselin myosin interactions would greatly expand the role that fesselin has within smooth muscle by placing it within the calcium signaling pathway. The regulation of fesselin-myosin interactions by calmodulin was tested using pyrene labeled actin as well as N,N'-Dimethyl-N-(Iodoacetyl)-N'-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Ethylenediamine labeled fesselin (IANBD-fesselin). These experiments showed that the interactions between fesselin and myosin are regulated by calmodulin. Overall, our results support that fesselin plays a critical role within smooth muscle to organize contractile elements.
East Carolina University
2014
Master's Thesis
http://hdl.handle.net/10342/4663
https://thescholarship.ecu.edu/bitstream/10342/4663/1/Kingsbury_ecu_0600O_11348.pdf
032b696c8fe3c15eafe5113f6b36293f
https://thescholarship.ecu.edu/bitstream/10342/4663/2/Kingsbury_NEDL.pdf
ac73b3640db19090d83618c88c720b99
https://thescholarship.ecu.edu/bitstream/10342/4663/3/Kingsbury_ecu_0600O_11348.pdf.txt
5819b2e16ebd472bdc5b14cba48bae83
Biochemistry
Molecular biology
Actin
Fesselin
Myosin
Rapid kinetics
Synaptopodin-2
Smooth Muscle Myosins--metabolism
Cytoskeletal Proteins--metabolism
Actins--metabolism
Adenosine Triphosphatases--metabolism
Membrane Proteins--physiology
Myosins--metabolism
oai:TheScholarship.intra.ecu.edu:10342/103472022-12-13T15:15:37Zcom_10342_73com_10342_122col_10342_117col_10342_124
Communication satisfaction as a component of job satisfaction : a comparison study between night and day nurses
Cooke, Valorie L.
Duldt, Bonnie Weaver
East Carolina University
Master's Thesis
en
http://hdl.handle.net/10342/10347
https://thescholarship.ecu.edu/bitstream/10342/10347/1/Cooke_Communication-satisfaction-as-a-component-of_1992.pdf
45c4b8bd8da38ec94047839d6f7498dc
https://thescholarship.ecu.edu/bitstream/10342/10347/2/Cooke_Communication-satisfaction-as-a-component-of_1992.pdf.txt
e258cce876296a9e945d18ee334db9a0
NURS
oai:TheScholarship.intra.ecu.edu:10342/68462022-10-03T17:05:36Zcom_10342_107com_10342_73com_10342_1col_10342_110col_10342_72
The Effect Of Fine Arts on Language and Literacy Skills in Children
Culver, Lauren
Walker, Marianna
Abstract
Current techniques of teaching literacy to elementary school students are commonly limited to the use of standard reading programs such as Macmillan, McGraw Hill and Houghton (LaJevic, 2013). Although systematic, these approaches do not allow for children to experience various ways of learning or engagement with these structured literacy programs. This study will utilize the constructivist approach to explore how children’s experiences and reflections enhance natural literacy development. The purpose of this study is to determine if creativity and engagement in the arts enhance language and literacy skills in children.
By integrating art practices with literacy tasks, specifically dance and visual arts, children will use such active experiences to enhance specific language and literacy skills. Children, who participate in artistic activities, will excel in subsequent oral and written language activities.
Current art integration in classrooms and has been minimal and undervalued. Funding for art programs have substantially decreased making this study significant and necessary in order to discuss benefits of arts in school settings.
The study was completed in a school in Greenville, North Carolina and will include five students who are at a second grade reading level. Pre-experimental language and literacy testing was completed before the study in the areas of spelling, reading, and oral language. Experimentally, the children have participated in listening, dance and visual art activities. Following each activity, each child was asked to generate words and statements relating to that activity. Dependent variables will include number and type (part of speech) of words generated (written), and number/type of written statements for each activity. Following each group activity, a group discussion was conducted followed by collection of data relating to word and sentence generation. Relationships of data to standardized measures of oral/written language were completed.
East Carolina University
2018-05-01
Honors Thesis
http://hdl.handle.net/10342/6846
https://thescholarship.ecu.edu/bitstream/10342/6846/1/CULVER-HONORSTHESIS-2018.pdf
aac9623cfdc0a973a19531da596cafc5
https://thescholarship.ecu.edu/bitstream/10342/6846/2/LICENSE.txt
db402c7dadcf50ecd6f96d7215b26c3a
https://thescholarship.ecu.edu/bitstream/10342/6846/4/CULVER-HONORSTHESIS-2018.pdf.txt
4406ff6eb6c19bb444b6495f0d0950c9
language, literacy, fine arts
oai:TheScholarship.intra.ecu.edu:10342/70712022-12-05T19:37:42Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
A COMPARISON OF THE EFFICACY OF KEYBOARDING WITHOUT TEARS PROGRAM AND MIXED METHOD INSTRUCTION
Branson, Sydney
Donica, Denise
The purpose of this study was to investigate the two-year impact of using the Keyboarding Without Tears[trademark] instruction program compared to a mixed method instruction approach on students' net typing words per minute (WPM) and technique. In the current technological era, it is becoming increasingly important for students to possess keyboarding skills so that they are successful in education, social activities, and future work. Although the Common Core State Standards (National Governors Association & Council of Chief School Officers, 2010) emphasize the use of technology, including keyboarding, across grades, there has been no systematic implementation of keyboarding in schools (Poole & Preciado, 2016). This is largely due to the fact that there is not enough research identifying how the effectiveness of keyboarding interventions would balance the cost of implementing these interventions. This study utilized a pre-test post-test design over a two-year intervention period to measure the effectiveness of the two different instruction programs. Results revealed that students in the Keyboarding Without Tears group had significant improvements in their keyboarding speed in second and third grade and their keyboarding technique in all grades as compared to students in the mixed methods group. Results also indicated that both forms of keyboarding curriculum are effective at significantly improving students' keyboarding speed and technique. Occupational therapists and teachers can utilize this information to support the implementation of a keyboarding curriculum in schools and improve the keyboarding skills of students that need improvement with this skill.
East Carolina University
2018-11-27
Master's Thesis
en
http://hdl.handle.net/10342/7071
https://thescholarship.ecu.edu/bitstream/10342/7071/1/BRANSON-MASTERSTHESIS-2018.pdf
c19a0486ae5c1b177a075186351e7b19
https://thescholarship.ecu.edu/bitstream/10342/7071/2/LICENSE.txt
8554278d31bc5efce2377e7f3e3df854
https://thescholarship.ecu.edu/bitstream/10342/7071/3/PROQUEST_LICENSE.txt
7ff32c43e1fa2ca859ef73ac3840fcd5
https://thescholarship.ecu.edu/bitstream/10342/7071/4/Branson%20thesis%20final%2010.17.18%20.pdf
6b9ade39c6b679b850369004f5a6423a
https://thescholarship.ecu.edu/bitstream/10342/7071/5/Sydney%20Branson%20Signatures%202018.pdf
2f00d90a6d565e09be67906e50c60772
https://thescholarship.ecu.edu/bitstream/10342/7071/6/Branson%20NEDL.pdf
f1853afb0ee2f606f42a905633031e16
https://thescholarship.ecu.edu/bitstream/10342/7071/8/BRANSON-MASTERSTHESIS-2018.pdf.txt
567586d87fca7ed0080298ec2cc0318c
Keyboarding
occupational therapy
elementary schools
Keyboarding
Psychomotor Performance
Handwriting
oai:TheScholarship.intra.ecu.edu:10342/39062021-03-03T20:53:58Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
The Effect of Drawing to Enhance Word Retrieval in Individuals with Chronic Severe Aphasia
Taylor, Anna Meredith
Hough, Monica Strauss
The purpose of the current study was to examine if individuals with severe chronic aphasia could increase their ability to retrieve object names by implementing the compensatory strategy of drawing. This unique treatment protocol was a combination of drawing and Semantic Feature Analysis. To date, there have been few other studies incorporating a word retrieval approach, such as SFA, and drawing.   Three individuals with chronic aphasia who had accompanying anomia participated in the study. Each participant underwent a pre-treatment training on the process of drawing which included: correctly holding a pencil/pen, moving one's hand around the paper, tracing objects, copying pictures of objects, and drawing pictures of objects from memory. The treatment protocol for each participant involved measurement of baseline performance, five days of individual treatment over a period of two weeks, and maintenance sessions. During the actual therapy sessions, treatment on drawing to name using a SFA cueing script was utilized. Participants were administered the Western Aphasia Battery-Revised and the Boston Naming Test-II pre- and post-treatment.   The results revealed that each participant demonstrated a clinically remarkable improvement in naming treatment pictures following the SFA treatment protocol; however, only Participant 1 showed some generalization of the retrieval process to untreated stimuli. Participants 2 and 3 demonstrated no generalization to untreated stimuli. Relative to drawing, Participant 1 showed a moderate increase in drawing abilities with some generalization to untreated stimuli. Participants 2 and 3 were able to proficiently draw the treatment and probe stimuli. Although all three participants showed some mild decrease on WAB-R AQ scores, scores on naming and word finding subtests were relatively constant. Participant 1 showed a clinically relevant increase on the BNT-II. Overall, the findings suggest that drawing may be a viable approach for improving word retrieval skills as well as a means for enhancing functional communication abilities in adults with severe, chronic aphasia. Â
East Carolina University
2012
Master's Thesis
http://hdl.handle.net/10342/3906
https://thescholarship.ecu.edu/bitstream/10342/3906/2/Taylor_ecu_0600M_10643.pdf.txt
405e9ecadb8f4b2c432d4bf7f24e0cf9
https://thescholarship.ecu.edu/bitstream/10342/3906/1/Taylor_ecu_0600M_10643.pdf
6134e3a4d5f71397ceceeff340bc1b8d
https://thescholarship.ecu.edu/bitstream/10342/3906/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/3906/4/Taylor_anna.pdf
f883988985b58b9dad5961b632a85c3b
Speech Therapy
Aphasia
Drawing
Neuropsychological Tests
Aphasia--therapy
oai:TheScholarship.intra.ecu.edu:10342/102022022-03-25T07:27:54Zcom_10342_73com_10342_122col_10342_117col_10342_124
The relationship between perception of risk and the implementation of universal precautions among licensed nurses
Nifong, Mary H.
Eason, Frances R.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10202
https://thescholarship.ecu.edu/bitstream/10342/10202/1/Nifong_The-relationship-between-perception-of-risk_1992.pdf
08dc45b6ae6baf44f1a74a08aaf99b3f
https://thescholarship.ecu.edu/bitstream/10342/10202/2/Nifong_The-relationship-between-perception-of-risk_1992.pdf.txt
033d3369e7608a25a2bee3b50398324a
NURS
oai:TheScholarship.intra.ecu.edu:10342/100582022-03-25T07:23:21Zcom_10342_73com_10342_122col_10342_117col_10342_124
The relationship between structured tuberculosis education and the development of self-care knowledge in the military health care setting : a pilot study
Aultowski, Glenda P.
Droes, Nellie S.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10058
https://thescholarship.ecu.edu/bitstream/10342/10058/1/Aultowski_The-relationship-between-structured-tuberculosis-education_1994.pdf
4e1cc5c039763eff7e33365225fd3215
https://thescholarship.ecu.edu/bitstream/10342/10058/2/Aultowski_The-relationship-between-structured-tuberculosis-education_1994.pdf.txt
d2309b0d01b91062c20d3ff3da4ee1cc
NURS
oai:TheScholarship.intra.ecu.edu:10342/68402022-09-30T12:12:48Zcom_10342_73com_10342_1col_10342_117col_10342_72
PAIN SUPPORT FROM FAMILY AND PROVIDERS IN THE DIABETIC AMPUTEE: A LIVED EXPERIENCE
Paul, Joanna
Horne , Carolyn
Chronic pain after lower extremity amputation surgery has been reported in up to 80% of patients. Amputations, which are among the most debilitating chronic complication of diabetes have a variety of consequences including increased depression, inability to perform daily activities, and perceived inability to reintegrate into society. This study sought to understand the lived experience of chronic pain among those who have undergone a diabetes-related lower limb amputation. Researchers used a qualitative empirical phenomenology design. Private, semi-structured interviews were conducted on a purposive sample (N = 11). Participants were primarily male (56%) with a mean age of 60.8 years (SD = +15.4). Interviews were transcribed verbatim. Transcribed interviews were read and re-read by each researcher separately and then compared to enhance credibility. Codes were identified for each participant separately and then across participants for common themes. Three major themes are highlighted by this research: 1) phantom pain is distinct from real amputation pain 2) sympathetic but not empathetic and 3) identification of a new normal. The first theme revealed that the amputee was hesitant to speak to others about their pain. Participants gravitated toward non-pharmacological treatments and discussed the need for alternatives. The second theme uncovered that family members had the desire to help but could not due to lack of understanding or knowing the experience of the amputee. Finally, identifying a new normal included the amputation being a choice and spirituality assisting with adjustment. This research has implications for how chronic neuropathic or phantom pain is managed among persons with amputations. More research is needed in identifying and teaching amputees alternative pain treatment beyond pharmacologic methods. This is especially important since amputees may be hesitant in discussing their pain with their healthcare provider and family if they feel the only solution is medication.
East Carolina University
2018-05-03
Honors Thesis
http://hdl.handle.net/10342/6840
https://thescholarship.ecu.edu/bitstream/10342/6840/1/PAUL-HONORSTHESIS-2018.pdf
f976b34b581727b76961d1c78e9b114c
https://thescholarship.ecu.edu/bitstream/10342/6840/2/LICENSE.txt
2b2fb1c11b2ed478dcb656dbf8876791
https://thescholarship.ecu.edu/bitstream/10342/6840/4/PAUL-HONORSTHESIS-2018.pdf.txt
0e07788432aa2fa4b1ce6c42fa0992e0
Amputation
Chronic pain
Diabetes
oai:TheScholarship.intra.ecu.edu:10342/49212021-03-03T20:56:35Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Discourse processing treatment for adults with aphasia.
Frisco, Nicole
Wright, Dr. Heather Harris
The general goal of treatment for people with aphasia (PWA) is to improve their ability to communicate, which involves the discourse level. Treatment at this level is not a new concept, and there are many well-established procedures that utilize the discourse approach.   Deficits that PWA exhibit during conversation are often undetectable by standardized testing. Although these deficits may not be detectable by standardized measures, they significantly impact the PWA's ability to maintain social relationships and engage in everyday communication. Multi-level analyses can identify strengths and weaknesses in PWA's discourse that relate to functional aspects of language processing and structural linguistics. Multi-level discourse analyses have revealed a more productive investigation of discourse production by more thoroughly documenting linguistic abilities in PWA.   The purpose of this study was to determine if using an intensive discourse processing treatment improved discourse production in adults with aphasia. Study aims included a) determining if the discourse processing treatment improved performance on measures of micro- and macro- linguistic processes for individuals with aphasia for trained and untrained discourse productions, and b) determining if treatment effects were maintained.   Participants included four PWA who met study criteria. The study included three phases: baseline, treatment, and maintenance. Baseline took place during week one of the study, treatment was during weeks two, three, and four, and the maintenance phase included data collection one week after treatment and one month post-treatment. Treatment involved twelve sequential picture stimuli and was administered in a four-step procedure.   A multi-level discourse analyses was applied to analyze changes in PWA communication. Results indicated that the discourse processing treatment resulted in improvements in participant's discourse for trained and untrained productions. The multi-level analysis was more beneficial than standardized measures for analyzing discourse and documenting change in response to treatment. Â
East Carolina University
2015
Master's Thesis
http://hdl.handle.net/10342/4921
https://thescholarship.ecu.edu/bitstream/10342/4921/1/Frisco_ecu_0600O_11431.pdf
7bdbbfb110a71cfad0afd703395ca518
https://thescholarship.ecu.edu/bitstream/10342/4921/2/FriscoNEDL.pdf
d5214750c882b66f78c464ddfe521709
https://thescholarship.ecu.edu/bitstream/10342/4921/3/Frisco_ecu_0600O_11431.pdf.txt
a0932ac19391d60872cae72bf78c80ff
Speech Therapy
Aphasia
Discourse
Treatment
Adult
oai:TheScholarship.intra.ecu.edu:10342/119712023-01-06T08:17:06Zcom_10342_73com_10342_122col_10342_117col_10342_124
Demographic characteristics, health beliefs, and preferred methods of smoking cessation interventions of prenatal military-related smokers
Eady, Mary Lee.
Lieber, Marilyn Therese.
East Carolina University
Masters Thesis
en
http://hdl.handle.net/10342/11971
https://thescholarship.ecu.edu/bitstream/10342/11971/1/Eady_Demographic-characteristics-health-beliefs-and-preferred_1994.pdf
e5c7189f47ab6271cb44046bae29b0a1
https://thescholarship.ecu.edu/bitstream/10342/11971/2/Eady_Demographic-characteristics-health-beliefs-and-preferred_1994.pdf.txt
8558acad9f239ab7b1a8f6710cb79a6b
NURS
oai:TheScholarship.intra.ecu.edu:10342/40612021-03-03T20:54:50Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
A comparison of drivers' braking responses across ages
Brown, Danielle
Dickerson, Anne Elizabeth
As driving is associated with independence, it is important for occupational therapists to understand what skills are required to drive safely and how to assess them in order to keep older drivers driving independently as long as possible. This study examined the reaction times and braking forces of younger and older adults in both simple and complex reaction situations. Results showed that all participants had an increase in reaction time with increased complexity of the situation. Although previous research has shown that reaction times slow with age, our results did not demonstrate statistically significant differences between the braking reactions of younger and older drivers. However, this study's results show that, the time difference between a brake reaction in a simple situation and one in a complex situation increases significantly with age (z = 2.364, p = 0.024). Additionally, younger and older drivers were able to consistently demonstrate application of sufficient force (e.g., 30 pounds) to control brake pedals. These findings support the concept that age related changes may influence the ability to process and react to complex stimuli. Â
East Carolina University
2012
Master's Thesis
http://hdl.handle.net/10342/4061
https://thescholarship.ecu.edu/bitstream/10342/4061/1/Brown_ecu_0600M_10844.pdf
2dcc671c90a4e600ba8367344fbb398f
https://thescholarship.ecu.edu/bitstream/10342/4061/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/4061/3/Brown_ecu_0600M_10844.pdf.txt
d0765c69a142ad3c1affdad403aace7a
Occupational Therapy
Aging
Driving
Driving simulator
Reaction force
Automobile driving simulators
Automobile Driving
Aging--physiology
Reaction Time
Cognition
Female
Male
Humans
Middle Aged
Adult
Occupational Therapy--methods
oai:TheScholarship.intra.ecu.edu:10342/102932022-03-26T07:19:09Zcom_10342_73com_10342_122col_10342_117col_10342_124
Self-efficacy and knowledge of AIDS among adolescents
Bobbitt, Deborah Carol
Horns, Phyllis N.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10293
https://thescholarship.ecu.edu/bitstream/10342/10293/1/Bobbitt_Self-efficacy-and-knowledge-of-AIDS_1992.pdf
06d478ee9985e7960b09adf75eddf5cf
https://thescholarship.ecu.edu/bitstream/10342/10293/2/Bobbitt_Self-efficacy-and-knowledge-of-AIDS_1992.pdf.txt
697a534ffd393d7bf47e8abd311201f5
NURS
oai:TheScholarship.intra.ecu.edu:10342/119702023-10-19T16:42:38Zcom_10342_73com_10342_122col_10342_117col_10342_124
Perceptions of head nurses regarding communication behaviors needed by nurses
Edwards, Rachel K.
Duldt, Bonnie W.
East Carolina University
Masters Thesis
en
http://hdl.handle.net/10342/11970
https://thescholarship.ecu.edu/bitstream/10342/11970/1/Edwards_Perceptions-of-head-nurses-regarding-communication_1988.pdf
9174ef9fee5eef5bb70f4339516175cf
https://thescholarship.ecu.edu/bitstream/10342/11970/2/Edwards_Perceptions-of-head-nurses-regarding-communication_1988.pdf.txt
99892acfa62994c657b46adc8c6742d4
https://thescholarship.ecu.edu/bitstream/10342/11970/4/Edwards_Permissions_Yes.pdf
393ff012bbd2edf2c7c286f7b896c07a
NURS
oai:TheScholarship.intra.ecu.edu:10342/44812021-03-03T20:56:42Zcom_10342_73com_10342_1col_10342_117col_10342_72
Are the Health Services for Children with Special Health Care Needs Met Through School Services?
Schillo, Rachel A.
Larson, Kim L.
The Maternal and Child Health Bureau recognizes children with special health care needs (CSHCN) as "those with chronic physical, developmental, behavioral, or emotional conditions that require health and related services of a type or amount beyond that required of children generally" (Kuhlthau, Bloom, Van Cleave, Knapp, Romm, Klatka... Perrin, 2011, p.136). These children may need medication, medical care, mental or educational services, or special therapy in order to maintain functionality (Houtrow, Okumura, Hilton, & Rehm, 2011). A significant number of CSHCN attend school daily who require additional health support (DurRant, Gibbons, Poole, Suessmann, & Wyckoff, 2010). Federal law mandates schools to adapt their services to provide equal opportunity for the student body including CSHCN, but many schools struggle to adapt programs due to cost of personnel and resources (Murphy & Carbone, 2008). When school services are not fully available to CSHCN, they are more likely to have unfavorable outcomes in school such as repeating grade, lack of engagement, and increased absences (Reuben & Pastor, 2013). To ensure that the health needs of children with disabilities are met adequately at schools, it is important to understand how schools provide comprehensive services for them (Reuben & Pastor, 2013). / This honors project was conducted in rural eastern North Carolina at a school-based health center in collaboration with a school nurse in Wayne County. The purpose of this project was to examine the health services available to the 81 CSHCN at Brogden Middle School and determine barriers to available health services. The objectives of this project were 1) to interview 4-5 key informants in the public school who have close contact with CSHCN by Feb 11, 2014 and 2) identify programs and services most utilized by CSHCN at this public school by Feb 17, 2014. Public health interventions used in this project were advocacy and policy enforcement. Written recommendations based on the findings will be provided to the Director of the school-based health center, school nurse, and key teachers to improve services for CHSCN in this school. /
2014
Undergraduate Thesis
http://hdl.handle.net/10342/4481
https://thescholarship.ecu.edu/bitstream/10342/4481/2/Schillo_Rachel.pdf
c3622907594b9bbd281c91599091f4c9
https://thescholarship.ecu.edu/bitstream/10342/4481/3/Schillo_Rachel.pdf.txt
3129512b28e4c55d095b6e34dc3ba0dc
Children with special health care needs
Health services
School services
oai:TheScholarship.intra.ecu.edu:10342/104602022-03-29T07:20:45Zcom_10342_73com_10342_122col_10342_117col_10342_124
A study of sibling visitation in a neonatal intensive care unit
Hager, Rhonda C.
Henning, Emilie D.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10460
https://thescholarship.ecu.edu/bitstream/10342/10460/1/Hager_A-study-of-sibling-visitation-in_1988.pdf
73e0a32c0f6b71adcc55757710e23904
https://thescholarship.ecu.edu/bitstream/10342/10460/2/Hager_A-study-of-sibling-visitation-in_1988.pdf.txt
29050c41da03175b1829065a56fe4e02
NURS
oai:TheScholarship.intra.ecu.edu:10342/100602022-07-05T13:08:14Zcom_10342_73com_10342_122col_10342_117col_10342_124
Examination of three dimensions of a nursing unit climate before and after a computerized bedside terminal system of documentation is activated
James, Elaine N.
Tranbarger, Russell E.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10060
https://thescholarship.ecu.edu/bitstream/10342/10060/1/James_Examination-of-three-dimensions-of-a_1994.pdf
b5445aa074edabe9c53ddc654e3559e7
https://thescholarship.ecu.edu/bitstream/10342/10060/2/James_Examination-of-three-dimensions-of-a_1994.pdf.txt
14162621124a9dec913f5df313420ad8
https://thescholarship.ecu.edu/bitstream/10342/10060/4/JamesElaineN.pdf
6a841128c8f4e5c4fa2e5ec9f1b5ef5c
NURS
oai:TheScholarship.intra.ecu.edu:10342/68612022-09-30T17:35:28Zcom_10342_73com_10342_1col_10342_117col_10342_72
Examining the Outcomes of a Community-Academic Partnership
Forbis, Kelly
Tyndall, Deborah
In the fall of 2013, a community-academic partnership was established between the ECU College of Nursing and Wells Elementary School in Wilson, North Carolina. Community-academic partnerships have been shown to provide opportunities for mutual academic and community service-related initiatives. Although there are many potential benefits to implementing these partnerships, research is limited on reported outcomes. Investigating these outcomes is critical to the development and sustainability of community-academic partnerships. The purpose of this study is to examine the outcomes of a four-year community-academic partnership between the College of Nursing and Wells Elementary School.
A single case study research design was used. Data was triangulated from multiple sources of evidence: interviews, physical artifacts, documentation, and participant observation. Purposive sampling was used to recruit individuals for interviews who participated directly or indirectly in the partnership. Ten participants provided consent to participate in audio-taped, semi-structured interviews. Data is being analyzed using content analysis. Preliminary findings suggest an overarching theme of reciprocity with two subthemes, mutual learning and engagement.
Preliminary findings from this study suggest reciprocity is a significant factor contributing to the sustainability of a community-academic partnership. Service learning opportunities and leadership development were areas both partners reported mutual benefit. Academic partners should identify innovative approaches to provide service learning and leadership opportunities that provide mutual benefit for community partners. Fostering and building relationships is also essential in the sustainability of a community-academic partnership.
East Carolina University
2018-05-03
Honors Thesis
http://hdl.handle.net/10342/6861
https://thescholarship.ecu.edu/bitstream/10342/6861/1/FORBIS-HONORSTHESIS-2018.pdf
3aa872e091b999de4e1425be2c7c0803
https://thescholarship.ecu.edu/bitstream/10342/6861/2/LICENSE.txt
300fa56753a4ff0da0673bb4965152db
https://thescholarship.ecu.edu/bitstream/10342/6861/4/FORBIS-HONORSTHESIS-2018.pdf.txt
1fd9df29c8cd812040528969c8e7f407
Community-Academic Partnership
Service Learning
Leadership
Case Study Research
oai:TheScholarship.intra.ecu.edu:10342/70162021-03-03T21:19:48Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Identification of Biomarkers in Non-Small Cell Lung Cancer Patients Treated with PD-1 Monoclonal Antibody Immunotherapy
Atwell, Druid Carlisle
Yang, Li V
Cancer immunotherapy works by taking a patient's existing immune system and priming it to recognize cancer cells in order for immune cells to mount an effective response to the disease. This is a less invasive means of treating cancer for the patient. However current immunotherapy does come with its own unique side effects such as auto immune disorders that manifest in the patients' treatment due to the blocking of essential immune regulatory checkpoints. In this study, patients are treated with drugs nivolumab and pembrolizumab, both of which are PD-1 (Programmed Death Receptor 1) monoclonal antibodies. These antibodies bind to PD-1 and prevent ligand interaction with PD-L1. PD-1 is a receptor expressed on the surface of activated B-cells, macrophages and T-cells. When PD-1 is activated by PD-L1 a signal propagates from the receptor to inside the cell that results in the apoptosis of the cell that expresses PD-1. The activation of PD-1 on activated T-cells ultimately results in a reduction of T-cell proliferation and IFN-[gamma] secretion. An apoptotic signal occurs through the inhibition of the cell survival signal that is propagated through the PI3K pathway. While there is knowledge on how the expression and activation of PD-1 on immune cells regulates the progression of cancer, there is a lack of evidence to suggest biomarkers in non-small cell lung cancer patients for optimizing immunotherapy. This study serves to identify biomarkers in non-small cell lung cancer patients undergoing PD-1 monoclonal antibody immunotherapy. To accomplish this, blood samples were collected from non-small cell lung cancer patients undergoing the immunotherapy treatment and the cell counts were taken. Cell types of interest include cytotoxic T-cells, helper T-cells, B-cells, and granulocytes. Cytotoxic T-cells were identified by CD8 expression, a known marker of cytotoxic T-cells. Helper T-cells were identified by CD4 expression and B-cells were identified by CD19 expression, both of which are known markers of helper T-cells and B-cells, respectively. Secondly, this study investigated the expression levels of known immune regulatory genes and how these changed over the course of the immunotherapy treatment. Known immune regulatory genes included PD-L1, PD-1, CTLA4, CD28, A2A, CD80 and CD86. The expression levels of the proton sensing family of G-protein coupled receptors (G2A, GPR4, OGR1 and TDAG8) were also investigated. Thirdly, we investigated how tumor cell expression of PD-1 and PD-L1 was altered when introduced into an acidic environment. Due to the tumor microenvironment being characteristically acidic this would provide insight on how anti PD-1 and anti PD-L1 immunotherapies could potentially be used in various cancers and may also lead to the development of potential future combination therapies. Our study shows that approximately 90% of patients exhibited an increase in cytotoxic T-cell counts with 50% of patients achieving healthy donor cytotoxic T-cell counts after receiving immunotherapy. Additionally 2 patients out of the total 16 patients achieved and sustained cytotoxic T-cell counts above that of healthy donors. There was an observable trend that indicated a possible correlation that PD-1 levels at baseline could predict patient response to the PD-1 monoclonal antibody immunotherapy. In addition to our research into the clinical aspects of PD-1 monoclonal antibody immunotherapy, we also investigated the change in expression of PD-1 and PD-L1 mRNA in several cancer cell lines. We observed that there was a variation in how cancer cells responded to acidosis. PD-1 and PD-L1 mRNA expression was shown to be regulated through several variables such as the acidity of the media, duration of exposure to acidic conditions and cancer cell type. It was also observed that there was PD-1 and PD-L1 expression in these cancer cell lines, at 5 hour and 24 hour treatment times, with a prominent level of PD-L1 mRNA expression in most of these cancer cells.
East Carolina University
2018-08-22
Master's Thesis
en
http://hdl.handle.net/10342/7016
https://thescholarship.ecu.edu/bitstream/10342/7016/1/ATWELL-MASTERSTHESIS-2018.pdf
8df50c2bfdb03ff828617a66f11f7484
https://thescholarship.ecu.edu/bitstream/10342/7016/2/PROQUEST_LICENSE.txt
dcd77021d7d25508ea4bf44e085f1b60
https://thescholarship.ecu.edu/bitstream/10342/7016/3/LICENSE.txt
4fa84c5a807ba44258fd43ef51faaf50
https://thescholarship.ecu.edu/bitstream/10342/7016/4/thesis%20signature%20page%208.9.18_1.jpg
5d58c826b6a5dba7c1e1bd8a96738a1a
https://thescholarship.ecu.edu/bitstream/10342/7016/5/thesis%20signature%20page%208.9.18.jpg
5d58c826b6a5dba7c1e1bd8a96738a1a
https://thescholarship.ecu.edu/bitstream/10342/7016/6/Thesis%20Defense%20Signature%20page%20CCI07302018.jpg
e1de49e9de53523150be21ae9255a6de
https://thescholarship.ecu.edu/bitstream/10342/7016/7/ECU%20Non-Exclusive%20Distribution%20License.pdf
cde49298c080b772d83efaad866412ce
https://thescholarship.ecu.edu/bitstream/10342/7016/9/ATWELL-MASTERSTHESIS-2018.pdf.txt
a84c27df15c882b7a35529b2bea42b0c
PD-1
PD-L1
Antibodies, Monoclonal
Carcinoma, Non-Small-Cell Lung
Lung Neoplasms
Immunotherapy
Humans
oai:TheScholarship.intra.ecu.edu:10342/29192021-03-03T20:52:52Zcom_10342_107com_10342_73com_10342_122col_10342_115col_10342_124
Facial Affect Recognition Accuracy in Inidividuals with Substance Use Disorders
Buckhout, Ashley
Toriello, Paul
Substance use, like many human behaviors, occurs along a broad continuum from no use to extremely heavy use. Satisfactory interpersonal interaction involves understanding others' facial affect. There is a lack of research on facial affect recognition accuracy in individuals with substance use disorders. This archival study will seek to explore individuals with substance use disorders facial affect recognition accuracy. This archival study was conducted with data from 10 Project Working Recovery consumers. These consumers had met the admission criteria for PWR, including being at least 18 years old, presenting with a substance use disorder, and being psychiatrically and medically stable enough to receive services at the intensive outpatient level. Data was collected as part of Project Working Recovery's clinical service; consumers were informed of the objectives, benefits, and possible inconveniences prior to completing the Diagnostic Analysis of Nonverbal Accuracy 2-Adult Facial Expressions (DANVA2-AF). The DANVA2-AF was administered and consumers were asked to identify the facial affect of 24 stimuli. Demographic data and results from the DANVA2-AF were analyzed to explore the study objective. Â
East Carolina University
2010
Master's Thesis
http://hdl.handle.net/10342/2919
https://thescholarship.ecu.edu/bitstream/10342/2919/3/Buckhout_ecu_0600M_10243.pdf.txt
df1d07f1702ec11802e2056d2a9d9fbd
https://thescholarship.ecu.edu/bitstream/10342/2919/2/embargo20110114.txt
bc949ea893a9384070c31f083ccefd26
https://thescholarship.ecu.edu/bitstream/10342/2919/1/Buckhout_ecu_0600M_10243.pdf
1f11cf43f2f252cc47452a5ca7e3339b
Health Sciences, Rehabilitation and Therapy
Substance-Related Disorders
Substance Abuse Detection--methods
oai:TheScholarship.intra.ecu.edu:10342/74662021-12-01T09:01:54Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_39
Feeding Practices in NC-based Early Head Start Programs among Teachers Serving 12-36mo Children
Bullard, Caitlin Elizabeth
Stage, Virginia
Background: Food behaviors are developed during the first few years of life making caregiver interactions especially important in the formation of healthy food preferences. Limited data exists on caregiver feeding styles and practices who work directly with toddlers 12-36 months. Of particular interest are differences in feeding practices among different ethnicities. Objective: To determine feeding styles of Early Head Start (EHS) teachers in North Carolina (NC) and examine differences in feeding practices by ethnicity. Design: Cross sectional data surveying the feeding practices of EHS teachers Participants/Setting: NC-based EHS teachers recruited throughout the state of NC aged 42.81 ± 12.74 working with toddlers 12-36 months. Main outcome measures: Validated instruments, Caregiver Feeding Style Questionnaire (CFSQ) and Mealtime Verbal Comments Checklist Statistical Analyses: Fisher's Exact Test, Mann-Whitney U Test, Pearson's [chi]^2, and Independent t-tests test were used to determine variations between ethnicities of childcare providers who completed the survey. Results: Teachers were categorized as authoritative (17.19%), authoritarian (34.38%), indulgent (29.69), and uninvolved (18.75%). Feeding styles were not significantly different among African Americans (AA) and White (p=0.33) teachers. However, significant differences were observed between individual items in the CFSQ such as "physically struggle with the child to get him or her to eat" (White [mu]=35.93 and AA [mu]=28.09; p=0.02). Statistical significant differences were also observed between 4 items in the Verbal Comments Checklist such as "when you're playing with your cup, you're showing me you're done" (p=0.045). White teachers were more likely to use both supportive (42.5±6.35) and unsupportive (57.48±6.34) comments during mealtime compared to AA with a mean of 37.24±12.69 for supportive comments and 62.76±12.69 unsupportive comments. Conclusions: Additional research is needed to explore direct observations of teacher-toddler interactions during mealtime to understand ethnic differences between teacher feeding styles.
East Carolina University
2019-07-22
Master's Thesis
en
http://hdl.handle.net/10342/7466
https://thescholarship.ecu.edu/bitstream/10342/7466/1/BULLARD-MASTERSTHESIS-2019.pdf
3f65fff683767571b610441b8abb62df
https://thescholarship.ecu.edu/bitstream/10342/7466/2/LICENSE.txt
708472c365dcf01158a5ec39210aab7e
https://thescholarship.ecu.edu/bitstream/10342/7466/3/PROQUEST_LICENSE.txt
932b69c98f055e0c7630741eb79e5060
https://thescholarship.ecu.edu/bitstream/10342/7466/4/Bullard_Signature%20Page.pdf
c9300618cd24f82aa9e7eab97679dda7
https://thescholarship.ecu.edu/bitstream/10342/7466/5/Signatures%20071819.pdf
a590f7c92a741be9bf8c42139765cbc9
https://thescholarship.ecu.edu/bitstream/10342/7466/6/Signatures%20071819_1.pdf
a590f7c92a741be9bf8c42139765cbc9
https://thescholarship.ecu.edu/bitstream/10342/7466/7/Bullard_License%20Document.pdf
85111e9d4456fee1b4c816cc57bd03dc
https://thescholarship.ecu.edu/bitstream/10342/7466/9/BULLARD-MASTERSTHESIS-2019.pdf.txt
3c78840d17c5324b44dd0352b05494ec
Feeding Styles
Ethnicity
Toddlers
Early Head Start Program
Feeding Styles
Educational Personnel
Early Intervention (Education)
Child
Feeding Behavior
oai:TheScholarship.intra.ecu.edu:10342/48232021-03-03T20:54:56Zcom_10342_73com_10342_1com_10342_2col_10342_117col_10342_72col_10342_8858
Evaluation of the Sexual Health Education of Teenage Males in a Middle School in Rural North Carolina
Dierolf, Karina
Larson, Kim L.
Teenage pregnancy has serious consequences for the young couple including limited education, inadequate employment opportunities, and adverse health outcomes (Alford & Houser, 2011). North Carolina is consistently among the nation’s leading states in teenage pregnancy and births. Teenage pregnancy has an enormous economic impact costing North Carolina taxpayers $325 million per year (The National Campaign to Prevent Teen and Unplanned Pregnancy, 2015). Although the most effective method of preventing pregnancy is to have both genders knowledgeable in contraceptive options and supportive of each other, pregnancy prevention and sexual health education has been disproportionately geared towards the female population (Starr, 2013). Adolescent males are receiving more education on the prevention of sexually transmitted infections than on pregnancy prevention, leading to gaps in their sexual health knowledge.
This project was conducted at a middle school in rural eastern North Carolina. The middle school has a population of 524, represented by African American (44.2%), Hispanic (37.5%), and White (11.6%) students. The purpose was to evaluate the sexual health education provided to adolescent males in this rural school setting. The Healthy Youth Act of 2009 mandated comprehensive sexual health education for students in grades 7-9 in all North Carolina public schools. The project addressed two public health interventions: collaboration and policy development and enforcement. A senior nursing student collaborated with a school nurse, a family nurse practitioner, a health educator, and a physical/health education teacher to conduct this evaluation. The project objectives were to 1) obtain data on sexual health information received by adolescent males using a three-tiered algorithm, 2) interview 4 key informants on their role in sexual health education with males, and 3) observe sexual health education classes. The major finding of this program evaluation was the assessment form (RAAPS) used in the school-based health center may not have the types of questions that help identify sexual health needs of boys and the school nurse may have other knowledge that influences the referral action. Additionally, the school has a higher than expected number of at-risk students, with sixty percent of selected males with a completed RAAPS referred for further sexual health education. A written report of findings will be provided to the school administration and the school-based center advisory board.
2015
Honors Thesis
http://hdl.handle.net/10342/4823
https://thescholarship.ecu.edu/bitstream/10342/4823/1/Dierolf%20-%20Final%20Thesis.doc
2cc1e4934437311fd9a226c8c46c1070
https://thescholarship.ecu.edu/bitstream/10342/4823/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/4823/3/Dierolf%20-%20Final%20Thesis.doc.txt
326e5e645427fdb55e66219b68ce6bf1
Sexual health
Health education
Teenage males
oai:TheScholarship.intra.ecu.edu:10342/11052021-03-03T20:52:36Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Priming Anxiety into People who Stutter: Arousal's Influence on Fluency
Hudock, Daniel Joseph
Kalinowski, Joseph Stanley
Many experimental psychologists (Bargh, Chen & Burrows, 1996; Berner & Maier, 2004; Bowers, 1999; Chartrand, VanBaaren & Bargh, 2006; Chen & Haviland-Jones, 2000; Hazlett, Dawson, Schell & Filion, 1990; Macky-Sim & Laing, 1981; Sato & Aoki, 2006; Wilkowski & Robinson, 2007) have examined priming's influence over behavior, cognition, and language across modalities. This idea of priming or influencing a behavior with subtle cues has revealed great effect on state anxiety and cognition. The change in state anxiety is relevant to stuttering and is sought due to the hypothesized interaction between the pathology and anxiety. It has been suggested that people who stutter (PWS) are more anxious and less confident than typical speakers (Daniels & Gabel, 2004; Guitar, 2003; Peters & Hulstijn, 1984). Yet this interaction of anxiety's influence on the degree and severity of stuttering has yet to be fully explored but is determined to have a strong correlation (Guitar, 2003; Miller & Watson, 1992; Peters & Hulstijn, 1984). However situational anxieties have shown to either increase (Craig, 1990) or have no effect (Armson, Foote, Witt, Kalinowski & Stuart, 1997; Kalinowski, Stuart, Wamsley & Rastatter, 1999) on dysfluencies in PWS. Though this study did not examine the effects of situations on anxiety it tried to examine a more basic unit of anxiety. This compartmentalized approach examined basic emotional priming's influence on arousal. It examined the relationship between physiologic and self-reported arousals' effect on fluency in PWS. This relationship was examined via priming tasks of silent readings of emotionally embedded analogous word sets then physiologic, self-reported arousal assessments and speech samples to assess priming's influence. Priming sets included positive, negative, and neutral conditions post baseline data. Conditions were randomly chosen and administered to influence altered arousal states in PWS. Experimental trials were separated into physiologic and speech sample collection phases. Initially during the physiologic phase participants remained seated and motionless while connected to skin conductance transducers and heart rate electrodes as they silently read the priming tasks. Post priming interstimulus phases of self reported arousal assessments via the Self Assessment Manikin (SAM) arousal scale (Lang, 1980) and regression to physiologic baseline were administered. The independent variable for this experiment was the priming condition, with dependent variables of physiologic arousal (skin conductance and heart rate), self-reported arousal (Self Assessment Manikin arousal scale Lang, 1980), and stuttered syllable counts. A significant difference was noted in stuttered syllables during the positive priming condition of the speech sample phase. This positive condition differed from all other conditions (Baseline, Negative, and Neutral) by the significant reduction of stuttered syllables per passage. These results are similar to Young, (1985) which explains the true baseline of (PWS) can never be increased, but only decreased. He describes the true baseline as PWS interacting in real situations not involved with the clinic environment.
East Carolina University
2008-11
Master's Thesis
en
http://hdl.handle.net/10342/1105
https://thescholarship.ecu.edu/bitstream/10342/1105/3/Hudock_ecu_0600M_10002.pdf.txt
a5b9ef22cae6fa22b865b6fd4f051746
https://thescholarship.ecu.edu/bitstream/10342/1105/2/license.txt
709f460da4acceff4d7499a459e97b6a
https://thescholarship.ecu.edu/bitstream/10342/1105/1/Hudock_ecu_0600M_10002.pdf
6e03f5ddfcb6f407c5409438abfffab0
Stuttering
Priming
Fluency
Dysfluency
Arousal
Anxiety
Health sciences
Speech Pathology
Stuttering
Speech-Language Pathology
oai:TheScholarship.intra.ecu.edu:10342/43442021-03-03T20:53:34Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Examining the use of the Shore Handwriting Screening to assess the handwriting skills of pre-kindergarteners
Schofield, Erin
Donica, Denise
The purpose of this study was to examine the use of the Shore Handwriting Screening (SHS; Shore, 2003) and the SHS Score Sheet, created by researchers at East Carolina University, and assess their relationship to a standardized fine motor assessment in the pre-kindergarten population. Standardized assessments to measure handwriting skills of pre-kindergarten students are lacking (Feder & Majnermer, 2003; Puranik & Lonigan, 2009; Rosenblum, Weiss, & Parush, 2009). A prominent reason students are referred to occupational therapy in school is because of handwriting difficulties and fine motor problems (Asher, 2006; Feder, Majnemer, & Synnes, 2000). In order to provide the most effective intervention for students, it is important that occupational therapy practitioners are appropriately evaluating students' skills. Pre-kindergarten students' scores on the SHS were compared to their scores on a portion of a standardized fine motor assessment, the Bruininks-Oseretsky Test of Motor Proficiency, Second Edition (BOT-2; Bruininks & Bruininks, 2005). Testing the SHS and BOT-2 across two socioeconomic groups provided an opportunity to compare the scores across a broad range of pre-kindergarten   students. Thirty-six students from a federally funded pre-kindergarten program and fourteen students from a private pre-kindergarten classroom completed the SHS and BOT-2 and scores were compared within and across the two classrooms. Results show that the SHS displayed moderate to strong correlations with three subtests of the BOT-2 (Fine Motor Precision, Fine Motor Integration, and Manual Dexterity). Students from the private pre-kindergarten classroom obtained higher scores on both the SHS and three of the four subtests of the BOT-2 in comparison to the federally funded pre-kindergarten students. The SHS, along with the SHS Score Sheet, is a functional and practical handwriting screening that shows evidence of being related to a standardized fine motor assessment. Â
East Carolina University
2013
Master's Thesis
http://hdl.handle.net/10342/4344
https://thescholarship.ecu.edu/bitstream/10342/4344/3/Schofield_ecu_0600M_11059.pdf.txt
347b09d23bcca8afcca6755d11e37f86
https://thescholarship.ecu.edu/bitstream/10342/4344/1/Schofield_ecu_0600M_11059.pdf
f3b3a782cda46143efe61e0fb0e47563
https://thescholarship.ecu.edu/bitstream/10342/4344/5/Schofield%2c%20Erin%20Elizabeth.pdf
cf93b29d2ea3e8a4855b687dcdee73e6
Occupational Therapy
Child, Preschool
Motor Skills--physiology
Handwriting
Occupational Therapy--methods
Social Class
oai:TheScholarship.intra.ecu.edu:10342/76252022-12-05T17:48:02Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
Matrix Metalloproteinase 12 is Critical for Granuloma Formation in the Murine Model of Granulomatous disease
Neequaye, Nicole N
Mohan, Arjun
Matrix Metalloproteinase 12 (MMP12) is a protein produced primarily by alveolar macrophages that degrades elastin in the extracellular matrix (ECM) and enables infiltration of immune cells that participate in the inflammatory response. To our knowledge, few studies have been conducted to clarify the role of MMP12 in granulomatous diseases such as sarcoidosis, a chronic inflammatory disease characterized by granuloma formation primarily in the lungs. Previous studies have shown an increase in gene and protein expression of MMP12 in lung tissue and bronchoalveolar lavage (BAL) of patients with sarcoidosis as well as a correlation between MMP12 elevation and disease severity. Our murine model uses multiwall carbon nanotubes (MWCNT) to mimic the characteristics (gene, protein expression, and granuloma formation) observed in sarcoidosis patients. Based on these observations, we hypothesized that MMP12 plays a role in the acute and late inflammatory response in pulmonary sarcoidosis. MMP12KO mice were used to address this hypothesis. Analysis of gene expression of BAL cells in C57BL/6 (wildtype) mice shows a significant elevation in MMP12 after oropharyngeal instillation of MWCNT at all time points (3, 10, 20, 60, 90 days). We observed similar trends in proinflammatory genes chemokine (C-C motif) ligand 2 (CCL2), matrix metalloproteinase 14 (MMP14), and interferon-gamma (IFNIÌ‚Ä‘) at all time points and osteopontin (OPN) at 20, 60, and 90 days. MMP12 protein levels increased in BAL cells at all time points. Evaluation of BAL cells from MMP12KO mice shows a similarity in the expression of all proinflammatory genes explored with wildtype at 10 days. CCL2 and MMP14, identified through gene expression profiling of the wildtype to be directly regulated by MMP12, is significantly reduced at 60 days in MMP12KO MWCNT instilled mice compared to wildtype. Histological analyses at 3, 10, 20, and 60 days shows increasing exacerbation in wildtype and continuous attenuation of granuloma formation in MMP12KO mice after exposure to MWCNT. A proposed mechanism for the reduction of granulomas at 60 days in MMP12KO, lead to an investigation into the relationship between MMP12, a pro-inflammatory mediator and PPAR[gamma], an anti-inflammatory modulator. Gene analysis showed a significant increase in MMP12 in PPAR[gamma]KO mice compared to wildtype and a substantial rise in PPARIÌ‚Ä‘ in MMP12KO mice compared to wildtype. Interestingly, MMP12 significantly increased, and PPAR[gamma] decreased dramatically in African American sarcoid patient's vs. controls when adjusted for race. MMP12 is seemingly instrumental in driving granuloma pathogenesis during inflammation. Evaluation of genes in MMP12KO mice suggests that the macrophage-secreted cytokines and matrix genes explored are necessary for granuloma formation. The significant increase in PPAR[gamma] intrinsically and after instillation with MWCNT in MMP12KO and its' decrease in wildtype mice after MWCNT instillation at 60 days suggests an inverse relationship between MMP12 and PPAR[gamma]. A reduction in granuloma formation in MMP12KO mice compared to wildtype supports a critical role for MMP12 in granuloma formation.
East Carolina University
2019-08-16
Master's Thesis
en
http://hdl.handle.net/10342/7625
https://thescholarship.ecu.edu/bitstream/10342/7625/1/NEEQUAYE-MASTERSTHESIS-2019.pdf
1e4c1a0f4cec43af1b9f4d9a566ed093
https://thescholarship.ecu.edu/bitstream/10342/7625/2/LICENSE.txt
c0184cee0c2d81b46385323a935dac4e
https://thescholarship.ecu.edu/bitstream/10342/7625/3/PROQUEST_LICENSE.txt
9e022dbe6b35e8be9c8bd9c51d15d555
https://thescholarship.ecu.edu/bitstream/10342/7625/4/scanned%20and%20signed%20signature%20page.pdf
7f2cced7e9ed3ffc04cb0fae52eeb307
https://thescholarship.ecu.edu/bitstream/10342/7625/5/corrected%20signature%20page.pdf
ba5482002a1f591cc8a20f9680095214
https://thescholarship.ecu.edu/bitstream/10342/7625/6/corrected%20signature%20page_1.pdf
ba5482002a1f591cc8a20f9680095214
https://thescholarship.ecu.edu/bitstream/10342/7625/7/Distribution%20License.pdf
6cbaeaf7b982959a4c3029d2a14d17c1
https://thescholarship.ecu.edu/bitstream/10342/7625/9/NEEQUAYE-MASTERSTHESIS-2019.pdf.txt
50a0c4f76716fda1005f29895bfe9924
sarcoidosis
MMP12
granulomas
inflammation
osteopontin
PPARg
Granuloma
Matrix Metalloproteinase 12
Mice
Disease Models, Animal
oai:TheScholarship.intra.ecu.edu:10342/44712021-03-03T20:58:00Zcom_10342_73com_10342_1col_10342_117col_10342_72
Comparison of Usual Versus Best Practice in Preventing and Managing Low Back Pain
Mosakowski, Joshua, H.
Larson, Kim L.
Musculoskeletal disorders contribute to 34% of work-related injuries in the United States that result in missed days from work (Bureau of Labor Statistics, 2013). Healthcare workers are at a particularly high risk of injuring themselves in the hospital setting. Low back pain (LBP) has a significant public health impact as back injuries have the highest rate of occurrence out of all musculoskeletal disorders. Risk factors for LBP include obesity, joint disease, poor posture, and poor lifting techniques. Two frequently used treatments for LBP, with some evidence of effectiveness, are chiropractic and massage therapy. Prevention is critical to reducing the incidence of LBP. Exercise programs that incorporate aerobic and strength-training have proven effective at preventing LBP (Hasan et al., 2010). Evidence-based guidelines for managing LBP include prescribing acetaminophen, educating the client, and treating in a primary care setting (Williams et al., 2010). / This project is being conducted at a local hospital in rural eastern North Carolina. This hospital has experienced a high incidence of work-related LBP. The purpose of this project is to compare usual practice with best practice for the prevention and management of LBP among hospital workers. Collaboration and policy development and enforcement are two public health interventions used in this project. This project is a collaborative partnership with an interdisciplinary team including the staff development nurse, employee health nurse, rehabilitation specialist, physical therapist, occupational therapist, and insurance coordinator. The two project objectives are 1) to interview and observe the work of 5-7 key informants who are involved in LBP prevention and management, and 2) to make 2-4 recommendations, based on findings, to the hospital administration on the prevention and management of LBP. In this program evaluation, data generated from key informants will be organized in a matrix to identify gaps between usual and best practice. In collaboration with the staff development nurse this data will be analyzed and interpreted to provide recommendations in the prevention and management of LBP by February 26, 2014. /
2014
Undergraduate Thesis
http://hdl.handle.net/10342/4471
https://thescholarship.ecu.edu/bitstream/10342/4471/2/Mosakowski_Joshua.pdf
4b8b2542b3eeabc16455774d64d40b3d
https://thescholarship.ecu.edu/bitstream/10342/4471/3/Mosakowski_Joshua.pdf.txt
34d3048fa392da737f08410d623db615
Low back pain
Back injuries
Hospital employees
Chiropractic
Massage therapy
oai:TheScholarship.intra.ecu.edu:10342/102282023-11-22T15:24:48Zcom_10342_73com_10342_122col_10342_117col_10342_124
The relationship between three methods of pushing and the duration of the second stage of labor
Prather, Caren J.
Lieber, Marilyn Therese
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10228
https://thescholarship.ecu.edu/bitstream/10342/10228/1/Prather_The-relationship-between-three-methods-of_1996.pdf
9f894fe54953d91b6b3a3655e2f3f314
https://thescholarship.ecu.edu/bitstream/10342/10228/2/Prather_The-relationship-between-three-methods-of_1996.pdf.txt
65cc97ac83290cf8cb9e106e00059aff
https://thescholarship.ecu.edu/bitstream/10342/10228/4/Prather_Caren_No_OA.pdf
b2975849f6e5ba1658cf4400edc25c6f
NURS
oai:TheScholarship.intra.ecu.edu:10342/28162022-12-09T19:39:09Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_39
FOOD OPTION WITH A MORE NUTRIENT FAVORABLE PROFILE IN GROCERY STORES : BUYING HEALTHIER FOODS WITHOUT PAYING MORE.
Mammarella, Sarah
Colby, Sarah E.
Obesity is more prevalent in people of lower-socioeconomic status. Since over half of meals consumed are prepared in the home, grocery store purchases may be playing an important role in the obesity epidemic. The objective of this study was to assess the price differences between more nutrient favorable (MNF; i.e. low in fat, low in sodium, low in sugar, low in calories and whole grain) foods and less nutrient favorable (LNF; i.e. high in fat, high in sodium, high in sugar, high in calories, and refined) food option pairs (n=126; 63 MNF, 63 LNF) in all grocery stores in Greenville, NC (n=13) in the summer of 2009. Anovas, independent t tests and post-hoc analyses were used to produce means for each food item across the 13 grocery stores. LNF food items never cost more than MNF food items. Of the MNF and LNF food pairs assessed, 14 (22.2%) of foods had statistically significant differences in price (P<0.01). When looked at for cent-for-cent differences, 33 (52.4%) of the food pairs assessed had no differences in price. The findings of this study show that consumers can largely reduce calorie, fat, sugar, and sodium content of grocery store food purchases without increasing cost.
East Carolina University
2010
Master's Thesis
en_US
http://hdl.handle.net/10342/2816
https://thescholarship.ecu.edu/bitstream/10342/2816/3/Mammarella_ecu_0600M_10128.pdf.txt
46fbf2c690737aaac003022777a852e5
https://thescholarship.ecu.edu/bitstream/10342/2816/2/embargo20110415.txt
bc949ea893a9384070c31f083ccefd26
https://thescholarship.ecu.edu/bitstream/10342/2816/1/Mammarella_ecu_0600M_10128.pdf
1ddc5db4d9796b18857315e4b0e86f5f
Health Sciences, Nutrition
Food prices--North Carolina--Greenville
Nutrition--Economic aspects
Obesity--Economic aspects
oai:TheScholarship.intra.ecu.edu:10342/76132021-12-01T09:01:54Zcom_10342_122com_10342_74com_10342_73col_10342_124col_10342_527
Development of small-molecule inhibitors of the initiating proteases, C1r and C1s, of the classical complement pathway
Rohlik, Denise L
Complement is a proteolytic cascade that upon activation plays a key effector role in the innate immune system and acts to prime the adaptive immune response. During normal homeostatic events, complement is tightly regulated for its roles in immune complex clearance, lysis of target cells, opsonization, and recruitment of leukocytes and monocytes to target areas. Several endogenous regulators are responsible for the control of complement activation, however when dysregulation occurs, aberrant complement activation has been linked to autoimmune, proinflammatory, and neurodegenerative diseases, including Alzheimer’s disease. Inhibition of the classical complement component C1 may ameliorate hallmarks of autoimmune and inflammatory disease.
The serine proteases within the C1 complex, C1r and C1s, are promising therapeutic targets for structure-based small-molecule drug development. We investigated the activity of a series of small-molecule compounds identified in a large-scale fragment library screen and those from a cheminformatics computational docking screen in which hit compounds were predicted to bind the C1r or C1s proteases. Using surface plasmon resonance and ELISA-based assays for hit validation, we analyzed the binding affinities and the inhibitory IC50’s of several compounds predicted to bind and inhibit the activation of C1r or C1s in a dose-dependent manner. In this study, we have identified four lead compounds (cmp-1611, cmp-1663, cmp-1696, cmp-1827) and their 10 active structural analogues that target and inhibit C1r activation.
Given their abilities to bind and inhibit C1r and favorable physicochemical properties, our lead compounds may provide a starting point for optimizing affinity and specificity necessary for developing novel routes of therapeutic upstream complement inhibition.
East Carolina University
2019-12-12
Master's Thesis
en
http://hdl.handle.net/10342/7613
https://thescholarship.ecu.edu/bitstream/10342/7613/1/ROHLIK-MASTERSTHESIS-2019.pdf
b88b910900c48c6aadda6e488b0136e6
https://thescholarship.ecu.edu/bitstream/10342/7613/2/LICENSE.txt
d227fa0ebd5fe3197623436eb014eab0
https://thescholarship.ecu.edu/bitstream/10342/7613/3/PROQUEST_LICENSE.txt
e6a5754f1105b92be124205ea1724df6
https://thescholarship.ecu.edu/bitstream/10342/7613/4/Committee%20Approval%20Form.pdf
43384a82fc2e61c67bab2f3450282170
https://thescholarship.ecu.edu/bitstream/10342/7613/5/Thesis%20Committee%20Approval%20Form.pdf
658c223a586cc8ae4d1974ccd7ccacfe
https://thescholarship.ecu.edu/bitstream/10342/7613/6/Thesis%20Committee%20Approval%20Form_1.pdf
658c223a586cc8ae4d1974ccd7ccacfe
https://thescholarship.ecu.edu/bitstream/10342/7613/7/Thesis%20Committee%20Approval%20Form_2.pdf
658c223a586cc8ae4d1974ccd7ccacfe
https://thescholarship.ecu.edu/bitstream/10342/7613/8/Non-exclusive%20dist%20form_1.pdf
9d8aeed32be1642048a311865ec442c2
https://thescholarship.ecu.edu/bitstream/10342/7613/10/ROHLIK-MASTERSTHESIS-2019.pdf.txt
bfe7a7a6b5e8c3ed34d4549fabed5b34
Complement
classical pathway
small-molecule inhibitor
C1r
C1s
oai:TheScholarship.intra.ecu.edu:10342/37262021-03-03T20:53:20Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Effects of a structured handwriting readiness program on the fine motor skills of children in head start
Winslow, Brittni
Donica, Denise
The purpose of this study was to investigate the effectiveness of a structured handwriting readiness program on improving fine motor skills of preschool children attending a rural Head Start. Pre- and post-testing of an experimental classroom and a control classroom was completed using the Fine Manual Control and Manual Coordination composites of the Bruininks-Oseretsky Test of Motor Proficiency Second Edition (BOT-2). The experimental classroom was exposed to the Fine Motor and Early Writing curriculum during center time in approximately four, 5-10 minute increments, twice a week for 16 weeks. Data analysis indicated that the experimental class had an increase greater than the control class in mean total point scores between pre-test and post-test in the Fine Motor Integration and Manual Dexterity subtests. The data also supported the use of the Fine Motor and Early Writing curriculum with preschool students with the largest deficit in fine motor skills by indicating an increase in scores for the lower quadrant of scores for the Fine Motor Control, Fine Motor Integration, Manual Dexterity and Upper-Limb Coordination subtests. Overall, data analysis suggests that the Fine Motor and Early Writing curriculum has a positive effect on the development of fine motor integration and manual dexterity skills in preschool children, and an even greater effect on the students with greater fine motor delay. Â
East Carolina University
2011
Master's Thesis
en_US
http://hdl.handle.net/10342/3726
https://thescholarship.ecu.edu/bitstream/10342/3726/2/Winslow_ecu_0600M_10541.pdf.txt
0368b1319a92705ecf4b8c3226c978a2
https://thescholarship.ecu.edu/bitstream/10342/3726/1/Winslow_ecu_0600M_10541.pdf
29dcb152a88382b00ec2a79707feda9b
https://thescholarship.ecu.edu/bitstream/10342/3726/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Education, Elementary
Teacher education
Fine motor skills
Head Start
Occupational Therapy
Child, Preschool
Early Intervention (Education)
Female
Handwriting
Humans
Male
Motor Skills--physiology
Occupational Therapy--methods
Psychological Tests
Treatment Outcome
Psychomotor Performance
oai:TheScholarship.intra.ecu.edu:10342/104762022-12-13T15:15:04Zcom_10342_73com_10342_122col_10342_117col_10342_124
A comparison of progressive relaxation technique to therapeutic touch in reducing state anxiety in hospitalized cardiovascular patients
Hooks, Patricia Pike
Duldt, Bonnie Weaver
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10476
https://thescholarship.ecu.edu/bitstream/10342/10476/1/Hooks_A-comparison-of-progressive-relaxation-technique_1988.pdf
149d57163b4b44d69bea25b04de5d5af
https://thescholarship.ecu.edu/bitstream/10342/10476/2/Hooks_A-comparison-of-progressive-relaxation-technique_1988.pdf.txt
73580f4664e6d8596d756f8f87d63b1b
NURS
oai:TheScholarship.intra.ecu.edu:10342/22332021-03-03T20:56:03Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Use of a Pressure Vest to Reduce the Physiological Arousal of People with Profound Intellectual and Physical Disabilities During Routine Nail Care
LaChappelle, Rebecca
Velde, Beth P.
This single subject ABAB study explored whether the use of a commercially available deep pressure vest would decrease physiological arousal in a male with profound mental retardation during nail care activities. Psychophysiological responses of electrodermal activity, skin temperature, electromyography, and heart rate were used as indicators of physiological arousal and recorded using the NeXus-10. Visual and statistical analysis revealed that the use of the deep pressure vest did not reduce physiological arousal during nail care. Â
East Carolina University
2009
Master's Thesis
en_US
http://hdl.handle.net/10342/2233
https://thescholarship.ecu.edu/bitstream/10342/2233/2/LaChappelle_ecu_0600M_10011.pdf.txt
32ff5ef2e5a17d43753ae5e7bd446a8e
https://thescholarship.ecu.edu/bitstream/10342/2233/1/LaChappelle_ecu_0600M_10011.pdf
0b7e7ad58a13bb2e70ec69ae5a194887
https://thescholarship.ecu.edu/bitstream/10342/2233/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Health sciences
Deep pressure
Rehabilitation and therapy
Mental retardation
Psychophysiology
Mentally Disabled Persons--rehabilitation
Arousal--physiology
Occupational Therapy--methods
oai:TheScholarship.intra.ecu.edu:10342/61612021-03-03T21:14:02Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
Vision Coach: Effects of Standing Versus Sitting on Visual Reaction Times
Miller, Megan E
Dickerson, Anne Elizabeth
Assessment and intervention tools for occupational therapy practice must be evidence-based for appropriate use and include normative data with healthy adults. The overall goal of this research was to collect normative data on healthy adults' visual reaction time when completing the full field 60 light task on a novel device, the Vision Coach. The specific research question in this study was to determine if a change in body positioning in regards to person's base of support will affect a person's reaction time. We hypothesized that reaction times would be significantly different in the positions of standing versus sitting. Reaction times from 121 healthy adults, ages ranging from 21-79 years, were collected. Participants completed eight trials total, four trials in a standing position, and four trials in a sitting position. There were no significant differences on the factors of body position, gender, height, and wingspan on the averaged visual reaction times. The implication is that clients can be standing or sitting for use of the tool and therapists have normative data available for usage. This research also provides foundational data for further studies on the Vision Coach apparatus as well baseline criteria for the process of standardization of the Vision Coach. Future studies will need to address the limitation of learning to determine the number of practice trials required in both positions.
East Carolina University
2017-05-03
Master's Thesis
en
http://hdl.handle.net/10342/6161
https://thescholarship.ecu.edu/bitstream/10342/6161/1/MILLER-MASTERSTHESIS-2017.pdf
dda4c7cee04c2eaa710c055ad556cc49
https://thescholarship.ecu.edu/bitstream/10342/6161/2/LICENSE.txt
3fdf90a5121d4b2d85d52a0e796a3e95
https://thescholarship.ecu.edu/bitstream/10342/6161/3/PROQUEST_LICENSE.txt
439e05989e240dce12cf8b9c626ea2f8
https://thescholarship.ecu.edu/bitstream/10342/6161/4/Signpage.pdf
ee9cbaefc51ae3aa3a321af7fa71d926
https://thescholarship.ecu.edu/bitstream/10342/6161/5/megandickersonsigned.pdf
bf9b39cc74b15fd723059b617c1f5822
https://thescholarship.ecu.edu/bitstream/10342/6161/7/MILLER-MASTERSTHESIS-2017.pdf.txt
3eeb7baddcffe4218e494753c4b2466b
Vision Coach
Reaction Time
Coach
Standing
Sitting
oai:TheScholarship.intra.ecu.edu:10342/68282022-09-30T17:36:23Zcom_10342_73com_10342_1col_10342_117col_10342_72
Examining Factors Associated with Physical Activity During Cardiac Rehabilitation
Grogan, Meagan
Crane , Patricia
Recurrent myocardial infarctions (MIs) account for a third of the incident rate for all MIs. Physical activity (PA) is an important health behavior to prevent a recurrent MI. Fatigue, sleepiness, anxiety, and depression are all barriers to PA. The purpose of this pilot study was to examine how fatigue tolerance, sleep, depression, and anxiety influenced PA in those attending cardiac rehabilitation (CR) after an MI or coronary artery bypass surgery.
Using a repeated measure design, we interviewed a pilot sample of 8 adults who were beginning CR. Each participant completed a demographic and health status form. A 100mm visual analog scale was used to determine fatigue tolerance. Sleep was measured using the Epworth Sleepiness Scale, the Patient Health Questionnaire-9 measured depression, and anxiety was measured using the Generalized Anxiety Disorder-7. Participants wore a Garmin activity tracker for one week and step data (PA) were calculated on full days of activity. All data were analyzed using Statistical Package for Social Sciences 25.
Most of the participants (N = 8) were White (88%), women (62.5%), and had a mean age of 70.5 (SD = 10.5). All had high cholesterol, 88% high blood pressure, and 38% had a history of smoking. The mean depression score was 7 (SD = 4.3; range 1-15) indicating mild depression. Sleep (M = 7.1; SD = 6) and anxiety (M = 4.88; SD = 5) were in normal ranges. Average number of daily steps was 6000 (SD = 2517; range = 2863-9526). The average fatigue that they could tolerate (M = 59; SD = 26.7) and the average fatigue that slowed them down (M = 49.6; SD = 24.7) were lower than their perceived normal fatigue level for people similar to them (M = 70.4; SD = 24). Average daily steps were negatively associated with fatigue level that slows them down (r = -.78) but none of the other fatigue tolerability measures correlated with PA.
Most are not reaching the amount of PA deemed to be cardioprotective. Because most indicated lower fatigue tolerance than what they perceived as normal, further study of fatigue tolerance and PA is warranted. A larger sample size is needed to determine the relationship of fatigue tolerance, sleep, depression and anxiety on PA.
East Carolina University
2018-05-03
Honors Thesis
http://hdl.handle.net/10342/6828
https://thescholarship.ecu.edu/bitstream/10342/6828/1/GROGAN-HONORSTHESIS-2018.pdf
11433ed861ff677fee6e674ff68974f4
https://thescholarship.ecu.edu/bitstream/10342/6828/2/Scan%20Apr%2025%2c%202018%20at%2011_39%20AM.pdf
da021b94260f0aa3ffcf8e24ff60bb16
https://thescholarship.ecu.edu/bitstream/10342/6828/3/LICENSE.txt
da1cb18a748ae9bbd5f8e35013c116ee
https://thescholarship.ecu.edu/bitstream/10342/6828/5/GROGAN-HONORSTHESIS-2018.pdf.txt
bbdc5f33273e53582bb453e9a626bed6
https://thescholarship.ecu.edu/bitstream/10342/6828/7/Scan%20Apr%2025%2c%202018%20at%2011_39%20AM.pdf.txt
81051bcc2cf1bedf378224b0a93e2877
Physical Activity
Cardiac Rehabilitation
oai:TheScholarship.intra.ecu.edu:10342/75902022-12-14T17:29:05Zcom_10342_74com_10342_73com_10342_122col_10342_3934col_10342_124
DEVELOPMENT OF SMALL-MODELCULE INHIGITORS OF THE INTIATING PROTEASES, C1r AND C1s, OF THE CLASSICAL COMPLEMENT PATHWAY
Rohlik, Denise
Garcia, Brandon L.
Complement is a proteolytic cascade that upon activation plays a key effector role in the innate immune system and acts to prime the adaptive immune response. During normal homeostatic events, complement is tightly regulated for its roles in immune complex clearance, lysis of target cells, opsonization, and recruitment of leukocytes and monocytes to target areas. Several endogenous regulators are responsible for the control of complement activation, however when dysregulation occurs, aberrant complement activation has been linked to autoimmune, proinflammatory, and neurodegenerative diseases, including Alzheimer's disease. Inhibition of the classical complement component C1 may ameliorate hallmarks of autoimmune and inflammatory disease. The serine proteases within the C1 complex, C1r and C1s, are promising therapeutic targets for structure-based small-molecule drug development. We investigated the activity of a series of small-molecule compounds identified in a large-scale fragment library screen and those from a cheminformatics computational docking screen in which hit compounds were predicted to bind the C1r or C1s proteases. Using surface plasmon resonance and ELISA-based assays for hit validation, we analyzed the binding affinities and the inhibitory IC50's of several compounds predicted to bind and inhibit the activation of C1r or C1s in a dose-dependent manner. In this study, we have identified four lead compounds (cmp-1611, cmp-1663, cmp-1696, cmp-1827) and their 10 active structural analogues that target and inhibit C1r activation. Given their abilities to bind and inhibit C1r and favorable physicochemical properties, our lead compounds may provide a starting point for optimizing affinity and specificity necessary for developing novel routes of therapeutic upstream complement inhibition.
2019-12
Thesis
en_US
http://hdl.handle.net/10342/7590
https://thescholarship.ecu.edu/bitstream/10342/7590/3/ROHLIK-MASTERSTHESIS-2019.pdf
b88b910900c48c6aadda6e488b0136e6
https://thescholarship.ecu.edu/bitstream/10342/7590/2/license.txt
8a4605be74aa9ea9d79846c1fba20a33
https://thescholarship.ecu.edu/bitstream/10342/7590/4/Rohlik_Non-exclusive%20dist%20form_1.pdf
9d8aeed32be1642048a311865ec442c2
https://thescholarship.ecu.edu/bitstream/10342/7590/5/Rohlik_Thesis%20Committee%20Approval%20Form_2.pdf
658c223a586cc8ae4d1974ccd7ccacfe
https://thescholarship.ecu.edu/bitstream/10342/7590/6/ROHLIK-MASTERSTHESIS-2019.pdf.txt
bfe7a7a6b5e8c3ed34d4549fabed5b34
small-molecule inhibitor
C1s
Complement Pathway, Classical
Peptide Hydrolases
Endopeptidases
Complement C1r
oai:TheScholarship.intra.ecu.edu:10342/18692021-03-03T20:52:43Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
The Effects of Social Stories on Language and Social Appropriateness in Children with Autism Spectrum Disorders
Taylor, Kelly M.
Heilmann, John
This study employed a single-subject design to determine the effects of Social Stories<super>TM</super> treatment approach on the language and social skills, specifically turn-taking, of four children with autism spectrum disorders within the context of a summer language camp through East Carolina University. Four language measures and five behavioral measures were obtained through activities targeting behaviors included within the context of the Social Story. Three of the four participants displayed an increase in language measures while all four participants displayed an increase in at least one of the behavioral measures associated with turn taking. Language performance is thought to have been influenced by scaffolding and depended, in part, upon the language levels of the participants. Behavioral outcomes suggest that Social Stories can be feasible in increasing positive behaviors as well as decreasing negative behaviors.
East Carolina University
2009
Master's Thesis
en_US
http://hdl.handle.net/10342/1869
https://thescholarship.ecu.edu/bitstream/10342/1869/2/Taylor_ecu_0600M_10013.pdf.txt
e713050ba60299ddda64401c556e9292
https://thescholarship.ecu.edu/bitstream/10342/1869/1/Taylor_ecu_0600M_10013.pdf
9a86ff42613221a7dd2c813f464ceda7
https://thescholarship.ecu.edu/bitstream/10342/1869/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Health Sciences, Speech Pathology
Speech communication
Health Sciences, Rehabilitation and Therapy
Autism
Language
Language camp
Social skills
Social stories
Turn taking
Autistic Disorder
Speech Pathology
oai:TheScholarship.intra.ecu.edu:10342/49202021-03-03T20:56:24Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Speech in noise ability, outer hair cell function, and working memory for trained flute players.
Caldwell, Kelly A.
Vermiglio, Andrew J.
The process of auditory speech recognition requires verbal ability, working memory, recall, and adequate auditory abilities to recognize speech. There is a well-known positive effect of musical training and experience on verbal working memory and speech recognition in noise compared to those without formal musical training. This study was conducted to determine the relationships between outer hair cell function, speech in noise ability, and working memory for flute players (N=12) and non-musician controls (N=10). The secondary purpose of this study is to determine the differences between flute players and matched controls on these three variables. Test included pure tone audiometry, distortion product otoacoustic emissions (DPOAEs), working memory, and three speech in noise tests. Significant group differences were found between HINT thresholds using four talker babble in the Noise Front and Noise Right conditions. Non-musician controls were found to demonstrate a significant relationship between HINT 4T NF and bilateral high frequency DPOAEs. Flute players were found to demonstrate a significant negative relationship between working memory and outer hair cell function for the left ear, and a significant negative relationship between right high frequency DPOAEs and years of experience. Incidentally, the flute player group reported more perceived difficulty hearing speech in noise than the non-musician control group despite higher mean high frequency DPOAE response amplitudes than the controls. These data imply that another auditory or cognitive factor contributes to perceived difficulty recognizing speech in the presence of noise.
East Carolina University
2015
Master's Thesis
http://hdl.handle.net/10342/4920
https://thescholarship.ecu.edu/bitstream/10342/4920/1/Caldwell_ecu_0600O_11455.pdf
2151f39e56b7f9b9710d15461d255876
https://thescholarship.ecu.edu/bitstream/10342/4920/2/CaldwellNEDL.pdf
f97d483df3c66cec463f2dc3acfde5a8
https://thescholarship.ecu.edu/bitstream/10342/4920/3/Caldwell_ecu_0600O_11455.pdf.txt
e67f0c5ec0832035be1bae0024c961b6
Audiology
Speech Therapy
Neuroscience
Auditory processing
Cognitive processing
Outer hair cells
Speech in noise
Working Memory
Speech Perception--physiology
Noise
Auditory Pathways--physiology
Memory, Short-Term--physiology
Perceptual Masking
Pitch Discrimination
Speech Reception Threshold Test
Otoacoustic Emission, Spontaneous
Music
oai:TheScholarship.intra.ecu.edu:10342/103802023-01-04T16:42:19Zcom_10342_73com_10342_122col_10342_117col_10342_124
Caring : a nursing administration phenomenon
Fogleman, Kathi B.
Lawler, Therese G.
East Carolina University
Thesis
en
http://hdl.handle.net/10342/10380
https://thescholarship.ecu.edu/bitstream/10342/10380/1/Fogleman_Caring-a-nursing-administration-phenomenon_1993.pdf
a014f5af999cba2a5b419a594b42beb6
https://thescholarship.ecu.edu/bitstream/10342/10380/2/Fogleman_Caring-a-nursing-administration-phenomenon_1993.pdf.txt
dbac5b05308274ee421975064a331b12
https://thescholarship.ecu.edu/bitstream/10342/10380/4/Fogleman_Permissions_Yes.pdf
0f8ae640ef5d9782258c57eb65c93a5b
NURS
oai:TheScholarship.intra.ecu.edu:10342/69832021-03-03T21:19:02Zcom_10342_122com_10342_74com_10342_73col_10342_124col_10342_100
Molecular Histology of EphrinA1 Expression in Mouse Heart Visualized Molecular Histology of EphrinA1 Expression in Mouse Heart Visualized with MALDI-MSI
Parks, Justin C.
Virag, Jitka A. I.
EphrinA1 is a tyrosine kinase receptor localized in the cellular membrane of healthy cardiomyocytes, the expression of which is lost upon myocardial infarction (MI). Intra-cardiac injection of the recombinant form of ephrinA1 (ephrinA1-Fc) at the time of ischemic injury in mice has shown beneficial effects by reducing tissue injury and resultant infarct size post-MI. To date, immunohistochemistry and Western blotting comprise the only experimental approaches utilized to localize and quantify relative changes of ephrinA1 in tissue sections and homogenates of whole left ventricle, respectively. Herein, we used matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) coupled with a time-of-flight mass spectrometer (MALDI/TOF MS) to identify intact ephrinA1 in cardiac tissue as well as ephrinA1 fragments that have undergone tryptic digestion. The next step for the characterization and understanding of ephrinA1's role in cardiac tissue was to develop an integrated quantitative method using MALDI-MSI technologies. For this thesis, an optimized a protocol for relative quantitation of endogenous tryptic ephrinA1 peptides detected in the healthy murine myocardium was developed using a standard curve generated with analytical standards. In healthy myocardium, there was approximately 50 ng of endogenous ephrinA1 per tissue section of 9.43 mm2 average area. MALDI-MSI thus provided a tool for the determination of not only anatomical distribution, but also relative quantitation of endogenous ephrinA1 in cardiac tissue, advancing our understanding of ephrinA1 expression profile in cardiac tissue. In order to further study ephrinA1 in cardiac tissue, MALDI-MSI was used to study the effects of ephrinA1-Fc treatment 1, 2, 4, and 7 days post-MI. In addition to studying the effects of ephrinA1-Fc on endogenous expression levels of ephrinA1 post-MI, we also inquired about the possibility of gender differences in response to injury as well as EphrinA1-Fc treatment.
East Carolina University
2018-07-25
Master's Thesis
en
http://hdl.handle.net/10342/6983
https://thescholarship.ecu.edu/bitstream/10342/6983/1/PARKS-MASTERSTHESIS-2018.pdf
a68efda66a83cecfc462c02afaae9c7c
https://thescholarship.ecu.edu/bitstream/10342/6983/2/PROQUEST_LICENSE.txt
42352e1ca790c03bf35c3406ae4a60ef
https://thescholarship.ecu.edu/bitstream/10342/6983/3/LICENSE.txt
754864f7569c0f6c595e3aab060d2c67
https://thescholarship.ecu.edu/bitstream/10342/6983/4/Thesis%20signatures%20with%20titles%202.0_2.pdf
ed694e6ad2f11a7a3c874c1819022e0b
https://thescholarship.ecu.edu/bitstream/10342/6983/5/Thesis%20signatures%20with%20titles%202.0_1.pdf
ed694e6ad2f11a7a3c874c1819022e0b
https://thescholarship.ecu.edu/bitstream/10342/6983/6/Thesis%20signatures%20with%20titles%202.0.pdf
ed694e6ad2f11a7a3c874c1819022e0b
https://thescholarship.ecu.edu/bitstream/10342/6983/7/Thesis%20signatures.pdf
ba3218b2cbecb3244530084dc5175738
https://thescholarship.ecu.edu/bitstream/10342/6983/8/Non-Exclusive-Distribution-License_jv.pdf
762486245c9960f8854bb4efd9d6da1d
https://thescholarship.ecu.edu/bitstream/10342/6983/9/Non-Exclusive-Distribution-License.pdf
05a586f84beaab5c0ac13906f0929b6f
https://thescholarship.ecu.edu/bitstream/10342/6983/11/PARKS-MASTERSTHESIS-2018.pdf.txt
7de0444868f988e172025b5a367a8206
MALDI-MSI
EphrinA1
Myocardial Infarction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Mice
Animals
Histological Techniques
oai:TheScholarship.intra.ecu.edu:10342/47732021-03-03T20:56:24Zcom_10342_73com_10342_1col_10342_117col_10342_72
Providing Access to Child Passenger Restraint Information: Is there a Role for Elementary Schools and Daycare Facilities?
Edwards, Meredith
Miles, Jane
The correct and appropriate use of a child passenger restraint system can increase the likelihood of a child surviving a motor vehicle crash and avoiding serious injury. Despite the benefits of the use of child passenger restraints, many parents do not use them correctly or at all, and one barrier that has prevented some parents from the correct use of passenger restraints for their children is not receiving updated information about correct use as their child grows up (Macy, Cunningham, Resnicow & Freed, 2014). This project was conducted at an elementary school and multiple daycare centers associated with an early childhood development program in eastern North Carolina. The purpose was to evaluate the need for an ongoing process at the elementary school and daycare facilities that could increase awareness and educate parents and guardians about appropriate use of passenger restraints as children grow. Collaboration with key informants included the daycare providers, a child care health consultant, the school nurse, administrators, teachers and staff at the school. The two objectives of this project were (1) to complete an evaluation of current practices for providing educational information to parents and guardians in the school and daycare facilities, and (2) to make recommendations, based on best practices, regarding the feasibility of establishing a process that would provide information on child passenger restraints through these facilities. It was found that little was being done to share child passenger safety information with parents, although facility and school staff stated that they would be willing to provide such information. Of 50 parents contacted by facility care providers, 52% had not received child passenger safety information in the last year, and 76% indicated they would like to receive more information. Based on the findings of the evaluation, recommendations were shared with daycare providers and elementary school teachers to establish a process to provide child passenger restraint information to parents.
2015
Honors Thesis
http://hdl.handle.net/10342/4773
https://thescholarship.ecu.edu/bitstream/10342/4773/2/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
https://thescholarship.ecu.edu/bitstream/10342/4773/1/Meredith%20Edwards%20senior%20honors%20project.doc
0137b11d05b9d4711db2218a937423f7
https://thescholarship.ecu.edu/bitstream/10342/4773/3/Meredith%20Edwards%20senior%20honors%20project.doc.txt
5ed948e63138d2443758ab7e96bfdf51
Child passenger restraints
Child safety
oai:TheScholarship.intra.ecu.edu:10342/60212021-03-03T21:10:15Zcom_10342_122com_10342_107com_10342_73col_10342_124col_10342_112
PERCEIVED EFFECTIVENESS OF A DRIVING AND COMMUNITY MOBILITY BOOTCAMP FOR INDIVIDUALS WITH AUTISM SPECTRUM DISORDER
Case, Rebecca
Dickerson, Anne Elizabeth
This study was designed to determine the perceived effectiveness of a driving and community mobility Bootcamp for young adults with high functioning autism spectrum disorder (HFASD). Seven participants and their parents completed surveys prior to and after the completion of the Bootcamp. They were asked a variety of questions about skills, knowledge, and functional abilities of the participant to perform driving and community mobility skills in both a likert scale and open-ended format. The results showed both parents and participants' perceived significant increases in the participant's abilities, knowledge and confidence. The participants and their parents also provided positive open-ended responses, comments, and opinions adding to the Bootcamp's success. Although only a survey on perceived changes, combined with objective data from other study elements, results suggest the Bootcamp was an effective intervention for increasing driving and community mobility skills for young adults with HFASD.
East Carolina University
2016-12-15
Master's Thesis
en
http://hdl.handle.net/10342/6021
https://thescholarship.ecu.edu/bitstream/10342/6021/1/CASE-MASTERSTHESIS-2016.pdf
fd4df6eec60443dfc8af2e47cc7eeeed
https://thescholarship.ecu.edu/bitstream/10342/6021/2/LICENSE.txt
e7af53f0b04961a0e9eb3db1754c5d60
https://thescholarship.ecu.edu/bitstream/10342/6021/3/PROQUEST_LICENSE.txt
cc4eac45c5001196aa1fa875f204eb02
https://thescholarship.ecu.edu/bitstream/10342/6021/4/Thesis%20Signatures.pdf
1bc23633b6800aaf66544c46d3072874
https://thescholarship.ecu.edu/bitstream/10342/6021/5/NEDL_Case_Thesis.pdf
0987b5739b7379aa91ef60daf3bc5959
https://thescholarship.ecu.edu/bitstream/10342/6021/7/CASE-MASTERSTHESIS-2016.pdf.txt
4f0ecb4d42a0f8b500fc511d829d7f93
Driving and Community Mobility, Instrumental Activity of Daily Living
Executive Function
Automobile Driving
Autism Spectrum Disorder
Humans
Perception
Residence Characteristics
oai:TheScholarship.intra.ecu.edu:10342/28242021-03-03T20:52:36Zcom_10342_107com_10342_73com_10342_122col_10342_110col_10342_124
Stability of conversational language samples from children in kindergarten: the effects of time, sample length, and topic
DeBrock, Lindsay
Heilmann, John
The purpose of this study was to examine the reliability of measures from conversational language samples in the school-aged population. Two 10-minute conversational language samples were collected from 20 at-risk kindergarten children to determine their reliability and feasibility as an assessment and progress monitoring tool. All samples were collected and transcribed by one examiner using an outlined elicitation protocol in which the children were asked open ended questions about school and home activities in various time segments. Test-retest reliability was determined across eleven language measures for each child. Significant reliability correlation coefficients were observed for each language sample measure. The amount of variance due to sample length and topic were analyzed utilizing generalizability theory, which observes various facets accounting for measurement error. Variance was calculated for each language measure. Conversational language samples were found to have strong test-retest reliability across all language measures. The greatest amount of variance in measures was attributed to the child in most language measures with the interaction between the child, session, and length/topic accounting for the second largest amount of variance in most   measures. Length and topic accounted for negligible amounts of variance in language measures. These findings demonstrated that shorter conversational language samples are reliable across time and that the length and topic of the sample do not have a substantial impact on the reliability of language measures. Â
East Carolina University
2010
Master's Thesis
en_US
http://hdl.handle.net/10342/2824
https://thescholarship.ecu.edu/bitstream/10342/2824/2/DeBrock_ecu_0600M_10111.pdf.txt
59756216b2032859ea48c8161397e6f2
https://thescholarship.ecu.edu/bitstream/10342/2824/1/DeBrock_ecu_0600M_10111.pdf
2f11c22f3dd7983f0624b70c4bf6d971
https://thescholarship.ecu.edu/bitstream/10342/2824/3/license.txt
48d772d9ef478e9dd063ea202fa5e0d9
Health Sciences, Speech Pathology
Speech-Language Pathology--methods
uketd_dc///com_10342_73/100