Browsing by Subject "Molecular biology"
Now showing items 1-20 of 65
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Analysis of Myopodin in Hela Cervical Carcinoma Cells
(East Carolina University, 2014)Synaptopodin-2 is a proline-rich, actin-binding protein that exists in many isoforms. Due to its large proline content, synaptopodin-2 exists in a naturally unfolded state and possesses the characteristics of a hub protein. ... -
Analysis of scn5Laa and scn5Lab Gene Function in Danio rerio (Zebrafish) Heart Development through TALENs/CRISPR-CAS9-mediated Gene Knockout
(East Carolina University, 2015)Research in our laboratory is focused on understanding the molecular developmental genetic effects of crude oil exposure on vertebrate embryogenesis. Our research has demonstrated that exposure of zebrafish embryos to ... -
Analysis of the hex regulon in Pseudomonas aeruginosa
(East Carolina University, 2012)Pseudomonas aeruginosa is an opportunistic pathogen whose defense mechanisms include biofilm production through carbohydrate metabolism. The activity of the enzymes in the hex regulon are required for catabolism of glucose ... -
Anti-Cancer Activity of Cucurbitacin IIa
(East Carolina University, 2012)Cancer is a debilitating disease resulting from uncontrolled proliferation. One major treatment strategy for cancer is the application of chemotherapeutic drugs which kill cancer cells. Cucurbitacins are a new family ... -
The bhuTUV and bhuO genes play vital roles in the ability of Brucella abortus to use heme as an iron source and are regulated in an iron-responsive manner by RirA and Irr
(East Carolina University, 2012)Brucella abortus is a Gram negative intracellular pathogen that causes the zoonotic disease brucellosis. Antibiotic treatment for brucellosis in humans is prolonged and sometimes followed by relapses. Currently, the United ... -
CaMKII Protein Expression and Phosphorylation in Mouse Skeletal Muscle Following Atrophy and Hypertrophy
(East Carolina University, 2012)The maintenance of skeletal muscle mass is vital for life, and elucidation of the molecular mechanisms that control this process is a critical first step towards the development of pharmaceutical treatments for muscle ... -
Characterization of MiR319-Regulated TCPs in Maize Development
(East Carolina University, 1/13/16)Maize produces two inflorescences, the tassel and the ear, that are essential for reproduction. Both inflorescences arise from similar inflorescence primordia and are patterned largely by the same developmental regulators. ... -
Characterization of Site-Directed Mutants in the Cytochrome c-550 Protein of Photosystem II
(East Carolina University, 2010)Photosynthesis is the process by which cyanobacteria, algae, and higher plants convert light energy to chemical energy via the biosynthesis of carbohydrates. Photosystem II is a multi-protein/pigment complex embedded in ... -
Characterization of the GINS subunit psf1 in Drosophila Melanogaster
(East Carolina University, 2010)GINS (Go Ichi Ni San; 5,1,2,3 in Japanese) is a heterotetrameric protein complex known to be essential for the establishment of DNA replication forks and the progression of the replisome. The GINS complex is known to be ... -
Characterization of Two Novel Mutants of DNA Polymerase Delta in Drosophila melanogaster
(East Carolina University, 2011)DNA is the genetic material for all living organisms which is constantly being unpackaged, replicated and repackaged. The replication of this genetic material involves numerous different proteins; however, DNA polymerase ... -
Characterizing the Expression Pattern of miR167-Regulated ZmArf3 and ZmArf30 in Maize Inflorescences
(East Carolina University, 2014)Plant growth and development depends on the activity of meristems, pools of stem cells that generate leaves and other organs. Maize (corn) produces two inflorescences, the tassel and ear, which are patterned by a series ... -
COMPARATIVE ANALYSIS OF TWO HUMAN CANCER CELL LINES FOLLOWING EXPOSURE TO LOW DOSE RADIATION
(East Carolina University, 2011)Over the past two decades, studies on the cellular response to very low dose radiation have been revolutionizing historically-held paradigms of cellular radioresponse. Indeed, a host of seemingly contradictory events have ... -
Comparison of ZnO nanoparticles and ZnCl₂ induced germ cell apoptosis in Caenorhabditis elegans
(East Carolina University, 2014)There is inadequate research data available on manufactured ZnO nanoparticles. Manufactured nanoparticles are widely used in various products and production of manufactured nanoparticles is becoming increasingly abundant. ... -
Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development
(East Carolina University, 2012)The different mechanisms involved in vertebrate synovial joint development are actively being uncovered. A variety of studies have thus far discovered the involvement of several large molecules in the processes of joint ... -
The contribution of motility and chemotaxis in the Borrelia burgdorferi infectious life cycle
(East Carolina University, 2013)Lyme disease has emerged as an increasing problem for people in the east and northeastern part of the United States. It can cause a chronic debilitating infection if left untreated and is difficult to diagnose. The illness ... -
[Delta]-catenin: implications in prostate cancer progression
(East Carolina University, 2014)Prostate cancer (PCa) is the most commonly diagnosed cancer and the second most common cause of cancer death among men in the US. Due to the advances in research, the ability to detect and cure PCa has improved and led to ... -
Development of Computational Tools and Resources for Cotton microRNA Analysis
(East Carolina University, 2014)MicroRNAs (miRNAs) are an extensive class of small regulatory RNAs which regulate gene expression at the posttranscriptional levels. miRNAs target genes for mRNA cleavage or translation inhibition based on the complementary ... -
Drosophila Ctf4 is essential for genome stability and normal cell cycle progression
(East Carolina University, 2010)Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding ... -
Effect of Duodenal-Jejunal Bypass on Skeletal Muscle Insulin Signaling in Goto-Kakizaki Rats
(East Carolina University, 2009)Gastric bypass surgery (RYGBP) for the treatment of obesity has proven to clinically reverse type 2 diabetes mellitus. RYGBP involves both gastric reduction and bypass of the proximal small intestine. Duodenal-Jejunal ... -
Effects of 5-fluorouracil Drug Treatment on the Expression Profile of MicroRNAs in MCF7 Breast Cancer Cells
(East Carolina University, 2010)Breast cancer is one of the leading causes of deaths in women worldwide. 5-flourouracil (5-FU) is a classic chemotherapeutic drug that has been widely used in the treatment of breast cancer patients. In this study, using ...