Author | Grammatikos, Alexandros P. | |
Author | Ghosh, Debjani | |
Author | Devlin, Amy | |
Author | Kyttaris, Vasileios C. | |
Author | Tsokos, George C. | |
Date Accessioned | 2016-06-14T13:54:21Z | |
Date Available | 2016-06-14T13:54:21Z | |
Date of Issue | 2013 | |
Identifier (Citation) | PLoS ONE; 8:8 p. 1-9 | en_US |
ISSN | 1932-6203 | |
Identifier (URI) | http://hdl.handle.net/10342/5577 | |
Description | Engagement of the CD3/T cell receptor complex in systemic lupus erythematosus (SLE) T cells involves Syk rather than the zeta-associated protein. Because Syk is being considered as a therapeutic target we asked whether Syk is central to the multiple aberrantly modulated molecules in SLE T cells. Using a gene expression array, we demonstrate that forced expression of Syk in normal T cells reproduces most of the aberrantly expressed molecules whereas silencing of Syk in SLE T cells normalizes the expression of most abnormally expressed molecules. Protein along with gene expression modulation for select molecules was confirmed. Specifically, levels of cytokine IL-21, cell surface receptor CD44, and intracellular molecules PP2A and OAS2 increased following Syk overexpression in normal T cells and decreased after Syk silencing in SLE T cells. Our results demonstrate that levels of Syk affect the expression of a number of enzymes, cytokines and receptors that play a key role in the development of disease pathogenesis in SLE and provide support for therapeutic targeting in SLE patients. | en_US |
Related URI | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0074550 | en_US |
Subject | T cells | en_US |
Subject | Blood donors | en_US |
Subject | Flow cytometry | en_US |
Subject | Cytokines | en_US |
Subject | Cytokines | en_US |
Title | Spleen Tyrosine Kinase (Syk) Regulates Systemic Lupus Erythematosus (SLE) T Cell Signaling | en_US |
Type | Article | en_US |
Identifier (PMID) | pmc3754955 | en_US |
Identifier (DOI) | 10.1371/journal.pone.0074550 | |
Journal Name | PLoS ONE | en_US |
Journal Volume | 8 | en_US |
Journal Issue | 8 | en_US |
Article Pages | 1-9 | en_US |