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Detection of Environmental DNA Damage Using Electrochemical Sensors and Mass Spectrometry
(East Carolina University, 2018-05-04)
Nickel exposure has been linked to a variety of health complications including instances of tumors and cancer in living organisms. Exposure to nickel can occur due to pollution from mining or industrial applications. ...
Heavy Metal Concentrations in Biofluid Samples and their Correlation to Metabolic Syndrome
(East Carolina University, 2018-05-01)
The goal of this project was to determine if toxic metals such as Cr, Mn, As, Ni, Cd, and Pb are stored in the adipose tissue of farm animals, in particular swine. To detect these trace elements, a method of extraction and ...
Membrane Localization of Pyrene-Trehalose Conjugates (PYRETs)
(East Carolina University, 2018-05-01)
The disaccharide trehalose is found in many organisms that can survive in extremely cold or dry environments. According to the “hydration hypothesis,” trehalose interacts with the glycerol/phosphate regions of lipid bilayers ...
Using LC/MS to Quantify Metabolites in Urine Samples Post Clinical Exposure to Benzoates in Beverages
(East Carolina University, 2018-05-01)
Evidence has suggested that benzoate consumption has the potential to cause weight loss prevention. Benzoate is conjugated with glycine in the mitochondrial matrix to form hippurate which is eliminated in urine. IRB approval ...
Elucidating the Mechanisms of Transthyretin Aggregation in Transthyretin Amyloidosis
(East Carolina University, 2018-07-24)
Accumulation of insoluble aggregates (amyloids) is a characteristic feature of many neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. Aggregation and deposition of Transthyretin (TTR) protein ...
Counting Protons: Using Isothermal Titration Calorimetry to Determine the Number of Protons Exchanged Upon Ca(II) and Cd(II) Binding to Wild-type HcTnC and Mutant C84A and C35A/C84A Proteins
(East Carolina University, 2018-07-20)
Maintaining a delicate balance of metal ions in the cell is crucial to the survival of many organisms. Divalent cadmium, Cd(II), is a non-essential metal ion that can disrupt this delicate balance and lead to deleterious ...
Using Fluorescence and Infrared Spectroscopy to Analyze the Interaction of Disaccharides with Lipid Bilayers
(East Carolina University, 2018-07-24)
It is well known that sugars, such as trehalose, dehydrate lipid membranes based upon the Water Replacement Hypothesis. Here, we have investigated the interaction between different sugars (sucralose, sucrose, and trehalose) ...
protaTETHER – a method for the incorporation of variable linkers in protein fusions reveals impacts of linker flexibility in a PKAc‐GFP fusion protein
(2018-03-14)
Protein fusions are of fundamental importance in the study of cellular biology and the elucidation of cell signaling pathways, and the importance of linkers for the proper function of protein fusions is well documented in ...
Fluorescent Derivatives of Prostamides: Tools for Studying Anti-Cancer Activity
(East Carolina University, 2018-07-23)
The endocannabinoid arachidonoyl ethanolamide (AEA) and prostaglandins derived from it selectively induce apoptosis in tumorigenic cell lines due to overexpression of COX-2. It is likely that a novel J-series prostamide, ...
THE DEVELOPMENT OF BETA-ACTIN MUTANTS WITH ALTERED BINDING AFFINITES FOR ATP AND ADP
(East Carolina University, 2018-12-10)
A number of neurodegenerative diseases are accompanied by the formation of cofilin-actin rods in neural cells. These rods can cause synaptic dysfunction and block transport within neurites. The overall goal of this project ...