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Selection of reliable reference genes in caenorhabditis elegans for analysis of nanotoxicity
(2012-03-15)
Despite rapid development and application of a wide range of manufactured metal oxide nanoparticles (NPs), the understanding of potential risks of using NPs is less completed, especially at the molecular level. The nematode ...
Genome-wide functional analysis of the cotton transcriptome by creating an integrated EST database
(2011-11-08)
A total of 28,432 unique contigs (25,371 in consensus contigs and 3,061 as singletons) were assembled from all 268,786 cotton ESTs currently available. Several in silico approaches [comparative genomics, Blast, Gene Ontology ...
MicroRNA expression analysis in the cellulosic biofuel crop switchgrass (panicum virgatum) under abiotic stress
(2012-03-28)
Switchgrass has increasingly been recognized as a dedicated biofuel crop for its broad adaptation to marginal lands and high biomass. However, little is known about the basic biology and the regulatory mechanisms of gene ...
Triclosan Disrupts SKN-1/Nrf2- Mediated Oxidative Stress Response in C. elegans and Human Mesenchymal Stem Cells
(2017-10-03)
Triclosan (TCS), an antimicrobial chemical with potential endocrine-disrupting properties, may pose a risk to early embryonic development and cellular homeostasis during adulthood. Here, we show that TCS induces toxicity ...
Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum
(2018-05-17)
A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4)
was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular
weight DNA, which was partially cleaved ...
Effects of aluminum oxide nanoparticles on the growth, development, and microrna expression of tobacco (nicotiana tabacum)
(2012-05-11)
Nanoparticles are a class of newly emerging environmental pollutions. To date, few experiments have been conducted to investigate the effect nanoparticles may have on plant growth and development. It is important to study ...
Nicotine exposure and transgenerational impact: a prospective study on small regulatory microRNAs
(2014-12-17)
Early developmental stages are highly sensitive to stress and it has been reported that pre-conditioning with tobacco smoking during adolescence predisposes those youngsters to become smokers as adults. However, the molecular ...
A high-efficiency CRISPR/Cas9 system for targeted mutagenesis in Cotton (Gossypium hirsutum L.)
(2017-03-03)
The complex allotetraploid genome is one of major challenges in cotton for repressing gene expression.
Developing site-specific DNA mutation is the long-term dream for cotton breeding scientists. The
clustered regularly ...
Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops
(2017-10-30)
Ten strains of endophytic diazotroph were isolated and identified from the plants collected from three different agricultural crop species, wheat, rice and maize, using the nitrogen-free selective isolation conditions. The ...
Differential expression of microRNAs during fiber development between fuzzless- lintless mutant and its wild-type allotetraploid cotton
(2017-01-31)
Cotton is one of the most important textile crops but little is known how microRNAs regulate cotton fiber development. Using a well-studied cotton fiberless mutant Xu-142-fl, we compared 54 miRNAs for their expression ...