Browsing Physics by Author "Setlow, Peter"
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Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus 2 Subtilis Cells Upon Prophage Induction During Spore Germination and 3 Outgrowth, and Vegetative Growth, Using Raman Tweezers and Live-Cell 4 Imaging
Wu, Mei-yan; Li, Yong-qing; Li, Wen-wei; Christie, Graham; Setlow, Peter (2021) -
Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics
Peng, Lixin; Chen, De; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2009-05-15)Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneously measure levels of Ca-dipicolinic acid (CaDPA) and changes in spore morphology and refractive index during germination ... -
How Moist Heat Kills Spores of Bacillus subtilis
Coleman, William H.; Chen, De; Li, Yong-Qing; Cowan, Ann E.; Setlow, Peter (East Carolina University, 2007-12)Populations of Bacillus subtilis spores in which 90 to 99.9% of the spores had been killed by moist heat gave only two fractions on equilibrium density gradient centrifugation: a fraction comprised of less dense spores ... -
Kinetics of Germination of Individual Spores of as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Zhou, Tingting; Dong, Zhiyang; Setlow, Peter; Li, Yong-Qing (2013)Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very heat resistant. Raman spectroscopy and differential interference contrast microscopy were used to monitor the kinetics of ... -
Levels of Ca2+-Dipicolinic Acid in Individual Bacillus Spores Determined Using Microfluidic Raman Tweezers
Huang, Shu-shi; Chen, De; Pelczar, Patricia L.; Vepachedu, Venkata Ramana; Setlow, Peter; Li, Yong-Qing (East Carolina University, 2007-07)Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a 1:1 chelate with calcium ion (Ca-DPA) comprises 5 to 15% of the dry weight of spores of Bacillus species. Ca-DPA is important in spore resistance to many ... -
Memory of Germinant Stimuli in Bacterial Spores
Wang, Shiwei; Faeder, James R.; Setlow, Peter; Li, Yong-Qing (2015-11)Bacterial spores, despite being metabolically dormant, possess the remarkable capacity to detect nutrients and other molecules in their environment through a biochemical sensory apparatus that can trigger spore germination, ... -
Role of Dipicolinic Acid in the Germination, Stability, and Viability of Spores of Bacillus subtilis
Magge, Anil; Granger, Amanda C.; Wahome, Paul G.; Setlow, Barbara; Vepachedu, Venkata R.; Loshon, Charles A.; Peng, Lixin; Chen, De; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2008-07)Spores of Bacillus subtilis spoVF strains that cannot synthesize dipicolinic acid (DPA) but take it up during sporulation were prepared in medium with various DPA concentrations, and the germination and viability of these ... -
Superdormant Spores of Bacillus Species Have Elevated Wet-Heat Resistance and Temperature Requirements for Heat Activation
Ghosh, Sonali; Zhang, Pengfei; Li, Yong-Qing; Setlow, Peter (East Carolina University, 2009-09)Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, D-glucose, or L-valine, respectively, germinate ... -
Uptake of and Resistance to the Antibiotic Berberine by Individual Dormant, Germinating and Outgrowing Spores as Monitored by Laser Tweezers Raman Spectroscopy
Wang, Shiwei; Yu, Jing; Suvira, Milomir; Setlow, Peter; Li, Yong-Qing (2015-12)Article Authors Metrics Comments Related Content Abstract Introduction Materials and Methods Results Discussion Supporting Information Acknowledgments Author Contributions References Reader Comments (0) Media ...