Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains
Author
Suárez-Esquivel, Marcela; Ruiz-Villalobos, Nazareth; Castillo-Zeledón, Amanda; Jiménez-Rojas, César; Roop, Martin R II; Comerci, Diego J.; Barquero-Calvo, Elías; Chacón-Díaz, Carlos; Caswell, Clayton; Baker, Kate S.; Chaves-Olarte, Esteban; Thomson, Nicholas R.; Moreno, Edgardo; Letesson, Jean J.; De Bolle, Xavier; Guzmán-Verri, Caterina
Abstract
Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing (WGS) analysis of the reference strain Brucella abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version at www.wikipedia.Comparison of this genome with two publically available 2308 genomes showed significant differences, particularly indels related to insertional elements, suggesting variability related to the transposition of these elements within the same strain. Considering the outcome of high resolution genomic techniques in the bacteriology field, the conventional concept of strain definition needs to be revised.
Date
2017-09-29
Citation:
APA:
Suárez-Esquivel, Marcela, & Ruiz-Villalobos, Nazareth, & Castillo-Zeledón, Amanda, & Jiménez-Rojas, César, & Roop, Martin R II, & Comerci, Diego J., & Barquero-Calvo, Elías, & Chacón-Díaz, Carlos, & Caswell, Clayton, & Baker, Kate S., & Chaves-Olarte, Esteban, & Thomson, Nicholas R., & Moreno, Edgardo, & Letesson, Jean J., & De Bolle, Xavier, & Guzmán-Verri, Caterina. (September 2017).
Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8466
MLA:
Suárez-Esquivel, Marcela, and Ruiz-Villalobos, Nazareth, and Castillo-Zeledón, Amanda, and Jiménez-Rojas, César, and Roop, Martin R II, and Comerci, Diego J., and Barquero-Calvo, Elías, and Chacón-Díaz, Carlos, and Caswell, Clayton, and Baker, Kate S., and Chaves-Olarte, Esteban, and Thomson, Nicholas R., and Moreno, Edgardo, and Letesson, Jean J., and De Bolle, Xavier, and Guzmán-Verri, Caterina.
"Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains". .
. (),
September 2017.
April 19, 2024.
http://hdl.handle.net/10342/8466.
Chicago:
Suárez-Esquivel, Marcela and Ruiz-Villalobos, Nazareth and Castillo-Zeledón, Amanda and Jiménez-Rojas, César and Roop, Martin R II and Comerci, Diego J. and Barquero-Calvo, Elías and Chacón-Díaz, Carlos and Caswell, Clayton and Baker, Kate S. and Chaves-Olarte, Esteban and Thomson, Nicholas R. and Moreno, Edgardo and Letesson, Jean J. and De Bolle, Xavier and Guzmán-Verri, Caterina,
"Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains," , no.
(September 2017),
http://hdl.handle.net/10342/8466 (accessed
April 19, 2024).
AMA:
Suárez-Esquivel, Marcela, Ruiz-Villalobos, Nazareth, Castillo-Zeledón, Amanda, Jiménez-Rojas, César, Roop, Martin R II, Comerci, Diego J., Barquero-Calvo, Elías, Chacón-Díaz, Carlos, Caswell, Clayton, Baker, Kate S., Chaves-Olarte, Esteban, Thomson, Nicholas R., Moreno, Edgardo, Letesson, Jean J., De Bolle, Xavier, Guzmán-Verri, Caterina.
Brucella abortus strain 2308 Wisconsin genome: importance of the definition of reference strains. .
September 2017;
():
.
http://hdl.handle.net/10342/8466. Accessed
April 19, 2024.
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