Hermann, Brian P.Cheng, KerenSingh, AnukritiRoa-De La Cruz, LorenaMutoji, Kazadi N.Chen, I-ChungGildersleeve, HeidiLehle, Jake D.Mayo, MaxWesternstroer, BirgitLaw, Nathan C.Oatley, Melissa J.Velte, Ellen K.Niedenberger, Bryan A.Fritze, DanielleSilber, ShermanGeyer, Christopher B.Oatley, Jon M.McCarrey, John R.2020-04-072020-04-072018-11-06http://hdl.handle.net/10342/7995Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduc- tion and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for aggregates of certain spermato- genic cell types, the full continuum of gene expres- sion patterns underlying ongoing spermatogenesis in steady state was previously unclear. Here, we cat- alog single-cell transcriptomes for >62,000 individual spermatogenic cells from immature (postnatal day 6) and adult male mice and adult men. This allowed us to resolve SSC and progenitor spermatogonia, eluci- date the full range of gene expression changes dur- ing male meiosis and spermiogenesis, and derive unique gene expression signatures for multiple mouse and human spermatogenic cell types and/or subtypes. These transcriptome datasets provide an information-rich resource for studies of SSCs, male meiosis, testicular cancer, male infertility, or contra- ceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve sper- matogenesis in vitro.Article10.1016/j.celrep.2018.10.026