Ito, Takeru2011-03-142011-05-172011-03-142011-05-171993-07Applied and Environmental Microbiology; 59:7 p. 2343-2345http://hdl.handle.net/10342/3303A specific enzymatic method to determine the amounts of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid (2,3-DHBA) was devised. A sample was incubated first with hippurate hydrolase andthenwith2,3-DHBA-3,4-dioxygenase. Itoicacidwas estimatedfromtheincreaseinA374.Theincubation with the first enzyme was omitted for the determination of 2,3-DHBA. Originally published Applied and Environmental Microbiiology, Vol. 59, No. 7, July 1993en-USAuthor notified of opt-out rights by Cammie Jennings.Ioic acidBacillus subtilis2,3-dihydroxybenzoic acidEnzymatic determination of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid.ArticlePMC182283