Ferguson, Gail P.Datta, AnupBaumgartner, JohnRoop, R. Martin IICarlson, Russ W.Walker, Graham C.2011-02-282011-05-172011-02-282011-05-172004-04-06Proceedings of the National Academy of Sciences; 101:14 p. 5012-5017http://hdl.handle.net/10342/3258Sinorhizobium meliloti, a legume symbiont, and Brucella abortus, a phylogenetically related mammalian pathogen, both require the bacterial-encoded BacA protein to establish chronic intracellular infections in their respective hosts. We found that the bacterial BacA proteins share sequence similarity with a family of eukaryotic peroxisomal-membrane proteins, including the human adrenoleukodystrophy protein, required for the efficient transport of verylong- chain fatty acids out of the cytoplasm. This insight, along with the increased sensitivity of BacA-deficient mutants to detergents and cell envelope-disrupting agents, led us to discover that BacA affects the very-long-chain fatty acid (27-OHC28:0 and 29-OHC30:0) content of both Sinorhizobium and Brucella lipid A. We discuss models for how BacA function affects the lipid-A fatty-acid content and why this activity could be important for the establishment of chronic intracellular infections. Originally published Proceedings of the National Academy of Sciences, Vol. 101, No. 14, Apr 2004en-USSinorhizobium melilotiBrucella abortusBacA proteinLipid fatty acidArticlePMC387365