Alcantara, Rosemarie B.Read, Richard D. A.Valderas, Michelle WrightBrown, Timothy D.Roop, R. Martin II2011-02-042011-05-172011-02-042011-05-172004-08Infection and Immunity; 72:8 p. 4911-4917http://hdl.handle.net/10342/3200Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display little or no attenuation in cultured murine macrophages or mice. These experimental findings confirm the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages. In contrast to previous reports, however, these results indicate that exogenous tryptophan and phenylalanine are available for use by the brucellae in the phagosomal compartment. Originally published Infection and Immunity, Vol. 72, No. 8, Aug 2004en-USAuthor notified of opt-out rights by Cammie JenningsPurine biosynthesis pathwaysBrucella abortusTryptophan usePhenylalanine useArticlePMC47068410.1128/IAI.72.8.4911-4917.2004