Browsing by Author "Roop, R. Martin II"
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Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing
Khan, Sharik R.; Gaines, Jennifer M.; Roop, R. Martin II; Farrand, Stephen K. (East Carolina University, 2008-08)Experiments requiring strong repression and precise control of cloned genes can be difficult to conduct because of the relatively high basal level of expression of currently employed promoters. We report the construction ... -
Deficiency of a Sinorhizobium meliloti bacA Mutant in Alfalfa Symbiosis Correlates with Alteration of the Cell Envelope
Ferguson, Gail P.; Roop, R. Martin II; Walker, Graham C. (East Carolina University, 2002-10)The BacA protein is essential for the long-term survival of Sinorhizobium meliloti and Brucella abortus within acidic compartments in plant and animal cells, respectively. Since both the S. meliloti and B. abortus bacA ... -
Genetic Organization and Iron-Responsive Regulation of the Brucella abortus 2,3-Dihydroxybenzoic Acid Biosynthesis Operon, a Cluster of Genes Required for Wild-Type Virulence in Pregnant Cattle
Bellaire, Bryan H.; Elzer, Philip H.; Hagius, Sue; Walker, Joel; Baldwin, Cynthia L.; Roop, R. Martin II (East Carolina University, 2003-04)Brucella abortus reportedly produces the monocatechol siderophore 2,3-dihydroxybenzoic acid (2,3-DHBA) in response to iron limitation. Nucleotide sequence analysis of the cloned DHBA biosynthesis locus from virulent B. ... -
Intact Purine Biosynthesis Pathways Are Required for Wild-Type Virulence of Brucella abortus 2308 in the BALB/c Mouse Model
Alcantara, Rosemarie B.; Read, Richard D. A.; Valderas, Michelle Wright; Brown, Timothy D.; Roop, R. Martin II (East Carolina University, 2004-08)Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALB/c mouse model, while isogenic mutants with mini-Tn5 insertions in pheA, trpB, and dagA display ... -
A Novel Formamidase is Required for Riboflavin Biosynthesis in Invasive Bacteria
Baumgartner, John; Pitzer, Josh E.; Roop, R. Martin II; Yurge, Svetlana N. (2002) -
Opsonized Virulent Brucella abortus Replicates within Nonacidic, Endoplasmic Reticulum-Negative, LAMP-1-Positive Phagosomes in Human Monocytes
Bellaire, Bryan H.; Roop, R. Martin II; Cardelli, James A. (East Carolina University, 2005-06)Cells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucellaspecific ... -
Production of the Siderophore 2,3-Dihydroxybenzoic Acid Is Required for Wild-Type Growth of Brucella abortus in the Presence of Erythritol under Low-Iron Conditions In Vitro
Bellaire, Bryan H.; Elzer, Philip H.; Baldwin, Cynthia L.; Roop, R. Martin II (East Carolina University, 2003-05)Production of the siderophore 2,3-dihyroxybenzoic acid (2,3-DHBA) is required for the wild-type virulence of Brucella abortus in cattle. A possible explanation for this requirement was uncovered when it was determined that ... -
RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst
Roux, Christelle M.; Booth, Natha J.; Bellaire, Bryan H.; Gee, Jason M.; Roop, R. Martin II; Kovach, Michael E.; Tsolis, Renee M.; Elzer, Philip H.; Ennis, Don G. (East Carolina University, 2006-07)Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in ... -
Similarity to peroxisomal-membrane protein family reveals that Sinorhizobium and Brucella BacA affect lipid-A fatty acids
Ferguson, Gail P.; Datta, Anup; Baumgartner, John; Roop, R. Martin II; Carlson, Russ W.; Walker, Graham C. (East Carolina University, 2004-04-06)Sinorhizobium meliloti, a legume symbiont, and Brucella abortus, a phylogenetically related mammalian pathogen, both require the bacterial-encoded BacA protein to establish chronic intracellular infections in their respective ...