Browsing by Subject "Pseudomonas aeruginosa"
Now showing items 1-18 of 18
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Adaptations of Pseudomonas aeruginosa to the Cystic Fibrosis Lung Environment Can Include Deregulation of zwf, Encoding Glucose-6-Phosphate Dehydrogenase
(East Carolina University, 2005-11)Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, ... -
Autolysis and Autoaggregation in Pseudomonas aeruginosa Colony Morphology Mutants
(East Carolina University, 2002-12)Two distinctive colony morphologies were noted in a collection of Pseudomonas aeruginosa transposon insertion mutants. One set of mutants formed wrinkled colonies of autoaggregating cells. Suppressor analysis of a subset ... -
Cloning and Characterization of the Pseudomonas aeruginosa zwf Gene Encoding Glucose-6-Phosphate Dehydrogenase, an Enzyme Important in Resistance to Methyl Viologen (Paraquat)
(East Carolina University, 1998-04)In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD1- or NADP1-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. ... -
Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa
(East Carolina University, 2002-12)A set of 30 mutants exhibiting reduced production of the phenazine poison pyocyanin were isolated following transposon mutagenesis of Pseudomonas aeruginosa PAO1. The mutants could be subdivided into those with defects in ... -
The Global Carbon Metabolism Regulator Crc Is a Component of a Signal Transduction Pathway Required for Biofilm Development by Pseudomonas aeruginosa
(East Carolina University, 2000-01)The transition from a planktonic (free-swimming) existence to growth attached to a surface in a biofilm occurs in response to environmental factors, including the availability of nutrients. We show that the catabolite ... -
The Influence of Iron on Pseudomonas aeruginosa Physiology: A regulatory link between iron and quorum sensing
(East Carolina University, 2008-06-06)In iron-replete environments, the Pseudomonas aeruginosa Fur (ferric uptake regulator) protein represses expression of two small regulatory RNAs encoded by prrF1 and prrF2. Here we describe the effects of iron and PrrF ... -
The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.
(East Carolina University, 1996-10)The gene (crc) responsible for catabolite repression control in Pseudomonas aeruginosa has been cloned and sequenced. Flanking the crc gene are genes encoding orotate phosphoribosyl transferase (pyrE) and RNase PH (rph). ... -
PqsE Functions Independently of PqsR-Pseudomonas Quinolone Signal and Enhances the rhl Quorum-Sensing System
(East Carolina University, 2008-11)Pseudomonas aeruginosa is an opportunistic pathogen that causes both acute and chronic infections in immunocompromised individuals. This gram-negative bacterium produces a battery of virulence factors that allow it to ... -
The Pseudomonas aeruginosa devB/SOL Homolog, pgl, Is a Member of the hex Regulon and Encodes 6-Phosphogluconolactonase
(East Carolina University, 2000-07)A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3- phosphate ... -
Pseudomonas aeruginosa PqsA Is an Anthranilate-Coenzyme A Ligase
(East Carolina University, 2008-02)Pseudomonas aeruginosa is an opportunistic human pathogen which relies on several intercellular signaling systems for optimum population density-dependent regulation of virulence genes. The Pseudomonas quinolone signal ... -
Pseudomonas aeruginosa relA Contributes to Virulence in Drosophila melanogaster
(East Carolina University, 2004-10)The stringent response is a mechanism by which bacteria adapt to nutritional deficiencies through the production of the guanine nucleotides ppGpp and pppGpp, produced by the RelA enzyme. We investigated the role of the ... -
The Pseudomonas Quinolone Signal Regulates rhl Quorum Sensing in Pseudomonas aeruginosa
(East Carolina University, 2000-05)The opportunistic pathogen Pseudomonas aeruginosa uses intercellular signals to control the density-dependent expression of many virulence factors. The las and rhl quorum-sensing systems function, respectively, through the ... -
The regulation of anthranilate synthesis for the production of pqs in pseudomonas aeruginosa
(East Carolina University, 2013)The ubiquitous bacterium P. aeruginosa is an important human pathogen that causes devastating infections in immunocompromised patients and chronic infections in cystic fibrosis patients. The ability of P. aeruginosa to ... -
SPECTROSCOPIC INVESTIGATIONS OF BINDING MECHANISMS BETWEEN ANTIMICROBIAL PEPTIDES AND MODELS OF THE MEMBRANES OF PSEUDOMONAS AERUGINOSA AND KLEBSIELLA PNEUMONIAE
(East Carolina University, 2014)The wide application of antibiotics goes back over sixty years to the first use of penicillin in the mid-1940s. Antimicrobial agents have well-documented activity and have played a significant role in defending against ... -
The Effects of the Unnatural Amino Acid TTO- 53 on the Biofilm of Staphylococcus aureus
(East Carolina University, 2023-07-18)There are over 30 different species of staphylococci and many cause infection that can lead to hospitalization or death. The member most associated with chronic infection is Staphylococcus aureus. Staphylococcus aureus is ... -
Transcriptional regulation of 'pqsR', a gene that encodes a regulator of quinolone signal synthesis and virulence in 'Pseudomonas aeruginosa'
(East Carolina University, 2015)The gram-negative bacterium 'Pseudomonas aeruginosa' is an opportunistic pathogen that frequently causes nosocomial infections and serious chronic lung infections in cystic fibrosis patients. During infection 'P. aeruginosa' ... -
Two Distinct Pathways Supply Anthranilate as a Precursor of the Pseudomonas Quinolone Signal
(East Carolina University, 2007-05)Pseudomonas aeruginosa is an opportunistic pathogen that causes serious infections in immunocompromised patients and those with cystic fibrosis (CF). This gram-negative bacterium uses multiple cell-to-cell signals to control ... -
A two-component response regulator, gltR, is required for glucose transport activity in Pseudomonas aeruginosa PAO1.
(East Carolina University, 1996-10)A 729-bp open reading frame (gltR) was identified in Pseudomonas aeruginosa PAO1 that encodes a product homologous to the two-component response regulator family of proteins. Disruption of gltR caused loss of glucose ...