Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains
Baulac, Stephanie; Lu, Hope; Strahle, Jennifer; Yang, Ting; Goldberg, Matthew S.; Shen, Jie; Schlossmacher; Lemere, Cynthia A.; Lu, Qun; Xia, Weiming
Mutations in the DJ-1 gene have been linked to autosomal recessive familial Parkinson's disease. To understand the function of DJ-1, we determined the DJ-1 expression in both zebrafish and post mortem human brains. We found that DJ-1 was expressed early during zebrafish development and throughout adulthood. Knock down (KD) of DJ-1 by injection of morpholino did not cause dramatic morphologic alterations during development, and no loss of dopaminergic neurons was observed in embryos lacking DJ-1. However, DJ-1 KD embryos were more susceptible to programmed cell death. While a slight reduction in staining for islet-1 positive neurons was observed in both DJ-1 KD and H2O2 treated embryos, the number of apoptotic cells was significantly increased in both KD and H2O2 treated embryos. Interestingly, DJ-1 expression was increased in brains of zebrafish under conditions of oxidative stress, indicating that DJ-1 is a part of stress-responsive machinery. Since oxidative stress is one of the major contributors to the development of Alzheimer's disease (AD), we also examined DJ-1 expression in AD brains. Using DJ-1 specific antibodies, we failed to detect a robust staining of DJ-1 in brain tissues from control subjects. However, DJ-1 immunoreactivity was detected in hippocampal pyramidal neurons and astrocytes of AD brains. Therefore, our results strongly suggest that DJ-1 expression is not necessary during zebrafish development but can be induced in zebrafish exposed to oxidative stress and is present in human AD brains. Originally published Molecular Neurodegeneration, Vol. 4, No. 12, Feb 2009
Baulac, Stephanie, & Lu, Hope, & Strahle, Jennifer, & Yang, Ting, & Goldberg, Matthew S., & Shen, Jie, & Schlossmacher, & Lemere, Cynthia A., & Lu, Qun, & Xia, Weiming. (February 2009). Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains. Molecular Neurodegeneration, 4(12), 1- 14. Retrieved from http://hdl.handle.net/10342/3217
Baulac, Stephanie, and Lu, Hope, and Strahle, Jennifer, and Yang, Ting, and Goldberg, Matthew S., and Shen, Jie, and Schlossmacher, and Lemere, Cynthia A., and Lu, Qun, and Xia, Weiming. "Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains". Molecular Neurodegeneration. 4:12. (1-14), February 2009. August 14, 2018. http://hdl.handle.net/10342/3217.
Baulac, Stephanie and Lu, Hope and Strahle, Jennifer and Yang, Ting and Goldberg, Matthew S. and Shen, Jie and Schlossmacher and Lemere, Cynthia A. and Lu, Qun and Xia, Weiming, "Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains," Molecular Neurodegeneration 4, no. 12 (February 2009), http://hdl.handle.net/10342/3217 (accessed August 14, 2018).
Baulac, Stephanie, Lu, Hope, Strahle, Jennifer, Yang, Ting, Goldberg, Matthew S., Shen, Jie, Schlossmacher, Lemere, Cynthia A., Lu, Qun, Xia, Weiming. Increased DJ-1 expression under oxidative stress and in Alzheimer's disease brains. Molecular Neurodegeneration. February 2009; 4(12): 1-14. http://hdl.handle.net/10342/3217. Accessed August 14, 2018.
East Carolina University