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    Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms

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    Author
    Jiao, Yongqin; Cody, George D.; Harding, Anna K.; Wilmes, Paul; Schrenk, Matthew; Wheeler, Korin E.; Banfield, Jillian F.; Thelen, Michael P.
    Abstract
    We examined the chemical composition of extracellular polymeric substances (EPS) extracted from two natural microbial pellicle biofilms growing on acid mine drainage (AMD) solutions. The EPS obtained from a mid-developmental-stage biofilm (DS1) and a mature biofilm (DS2) were qualitatively and quantitatively compared. More than twice as much EPS was derived from DS2 as from DS1 (approximately 340 and 150 mg of EPS per g [dry weight] for DS2 and DS1, respectively). Composition analyses indicated the presence of carbohydrates, metals, proteins, and minor quantities of DNA and lipids, although the relative concentrations of these components were different for the two EPS samples. EPS from DS2 contained higher concentrations of metals and carbohydrates than EPS from DS1. Fe was the most abundant metal in both samples, accounting for about 73% of the total metal content, followed by Al, Mg, and Zn. The relative concentration profile for these metals resembled that for the AMD solution in which the biofilms grew, except for Si, Mn, and Co. Glycosyl composition analysis indicated that both EPS samples were composed primarily of galactose, glucose, heptose, rhamnose, and mannose, while the relative amounts of individual sugars were substantially different in DS1 and DS2. Additionally, carbohydrate linkage analysis revealed multiply linked heptose, galactose, glucose, mannose, and rhamnose, with some of the glucose in a 4-linked form. These results indicate that the biochemical composition of the EPS from these acidic biofilms is dependent on maturity and is controlled by the microbial communities, as well as the local geochemical environment. Originally published Applied and Environmental Microbiology, Vol. 76, No. 9, May 2010
    URI
    http://hdl.handle.net/10342/3307
    Subject
     Extracellular polymeric substances; Acidic biofilms; Microbial communities; Geochemical environment 
    Date
    2010-05
    Citation:
    APA:
    Jiao, Yongqin, & Cody, George D., & Harding, Anna K., & Wilmes, Paul, & Schrenk, Matthew, & Wheeler, Korin E., & Banfield, Jillian F., & Thelen, Michael P.. (May 2010). Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms. Applied and Environmental Microbiology, 76(9), 2916- 2922. Retrieved from http://hdl.handle.net/10342/3307

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    MLA:
    Jiao, Yongqin, and Cody, George D., and Harding, Anna K., and Wilmes, Paul, and Schrenk, Matthew, and Wheeler, Korin E., and Banfield, Jillian F., and Thelen, Michael P.. "Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms". Applied and Environmental Microbiology. 76:9. (2916-2922), May 2010. February 21, 2019. http://hdl.handle.net/10342/3307.
    Chicago:
    Jiao, Yongqin and Cody, George D. and Harding, Anna K. and Wilmes, Paul and Schrenk, Matthew and Wheeler, Korin E. and Banfield, Jillian F. and Thelen, Michael P., "Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms," Applied and Environmental Microbiology 76, no. 9 (May 2010), http://hdl.handle.net/10342/3307 (accessed February 21, 2019).
    AMA:
    Jiao, Yongqin, Cody, George D., Harding, Anna K., Wilmes, Paul, Schrenk, Matthew, Wheeler, Korin E., Banfield, Jillian F., Thelen, Michael P.. Characterization of Extracellular Polymeric Substances from Acidophilic Microbial Biofilms. Applied and Environmental Microbiology. May 2010; 76(9): 2916-2922. http://hdl.handle.net/10342/3307. Accessed February 21, 2019.
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