Identification of E2F1 as a positive transcriptional regulator for delta-catenin
Author
Kim, Kwonseop; Oh, Minsoo; Ki, Hyunkyoung; Wang, Tao; Bareiss, Sonja; Fini, M. Elizabeth; Li, Dawei; Lu, Qun
Abstract
Delta-Catenin is upregulated in human carcinomas. However, little is known about the potential transcriptional factors that regulate delta-catenin expression in cancer. Using a human delta-catenin reporter system, we have screened several nuclear signaling modulators to test whether they can affect delta-catenin transcription. Among beta-catenin/LEF-1, Notch1, and E2F1, E2F1 dramatically increased delta-catenin-luciferase activities while beta-catenin/LEF-1 induced only a marginal increase. Rb suppressed the upregulation of delta-catenin-luciferase activities induced by E2F1 but did not interact with delta-catenin. RT-PCR and Western blot analyses in 4 different prostate cancer cell lines revealed that regulation of delta-catenin expression is controlled mainly at the transcriptional level. Interestingly, the effects of E2F1 on delta-catenin expression were observed only in human cancer cells expressing abundant endogenous delta-catenin. These studies identify E2F1 as a positive transcriptional regulator for delta-catenin, but further suggest the presence of strong negative regulator(s) for delta-catenin in prostate cancer cells with minimal endogenous delta-catenin expression. Originally published Biochem Biophys REs Commun, Vol. 369, No. 2, May 2008
Subject
Date
2008-05-02
Citation:
APA:
Kim, Kwonseop, & Oh, Minsoo, & Ki, Hyunkyoung, & Wang, Tao, & Bareiss, Sonja, & Fini, M. Elizabeth, & Li, Dawei, & Lu, Qun. (May 2008).
Identification of E2F1 as a positive transcriptional regulator for delta-catenin.
Biochemical and Biophysical Research Communications,
369(2),
414-
420. Retrieved from
http://hdl.handle.net/10342/3324
MLA:
Kim, Kwonseop, and Oh, Minsoo, and Ki, Hyunkyoung, and Wang, Tao, and Bareiss, Sonja, and Fini, M. Elizabeth, and Li, Dawei, and Lu, Qun.
"Identification of E2F1 as a positive transcriptional regulator for delta-catenin". Biochemical and Biophysical Research Communications.
369:2. (414-420),
May 2008.
April 26, 2024.
http://hdl.handle.net/10342/3324.
Chicago:
Kim, Kwonseop and Oh, Minsoo and Ki, Hyunkyoung and Wang, Tao and Bareiss, Sonja and Fini, M. Elizabeth and Li, Dawei and Lu, Qun,
"Identification of E2F1 as a positive transcriptional regulator for delta-catenin," Biochemical and Biophysical Research Communications 369, no.
2 (May 2008),
http://hdl.handle.net/10342/3324 (accessed
April 26, 2024).
AMA:
Kim, Kwonseop, Oh, Minsoo, Ki, Hyunkyoung, Wang, Tao, Bareiss, Sonja, Fini, M. Elizabeth, Li, Dawei, Lu, Qun.
Identification of E2F1 as a positive transcriptional regulator for delta-catenin. Biochemical and Biophysical Research Communications.
May 2008;
369(2):
414-420.
http://hdl.handle.net/10342/3324. Accessed
April 26, 2024.
Collections
Publisher
East Carolina University