Nectin like-5 overexpression correlates with the malignant phenotype in cutaneous melanoma
Bevelacqua, Valentina; Bevelacqua, Ylenia; Candido, Saverio; Skarmoutsou, Evangelia; Amoroso, Alfredo; Guarneri, Claudio; Strazzanti, Angela; Gangemi, Pietro; Mazzarino, Maria C.; D'Amico, Fabio; McCubrey, James A.; Libra, Massimo; Malaponte, Grazia
NECL-5 is involved in regulating cell–cell junctions, in cooperation with cadherins, integrins and platelet-derived growth factor receptor, that are essential for intercellular communication. Its role in malignant transformation was previously described. It has been reported that transformation of melanocytes is associated with altered expression of adhesion molecules suggesting the potential involment of NECL-5 in melanoma development and prognosis. To shed light on this issue, the expression and the role of NECL-5 in melanoma tissues was investigated by bioinformatic and molecular approaches. NECL-5 was up-regulated both at the mRNA and the protein levels in WM35, M14 and A375 cell lines compared with normal melanocytes. A subsequent analysis in primary and metastatic melanoma specimens confirmed “in vitro” findings. NECL-5 overexpression was observed in 53 of 59 (89.8%) and 12 of 12 (100%), primary melanoma and melanoma metastasis, respectively; while, low expression of NECL-5 was detected in 12 of 20 (60%) benign nevi. A significant correlation of NECL-5 overexpression was observed with most of known negative melanoma prognostic factors, including lymph-node involvement (P = 0.009) and thickness (P = 0.004). Intriguingly, by analyzing the large series of melanoma samples in the Xu dataset, we identified the transcription factor YY1 among genes positively correlated with NECL-5 (r = 0.5). The concordant computational and experimental data of the present study indicate that the extent of NECL-5 expression correlates with melanoma progression.
Bevelacqua, Valentina, & Bevelacqua, Ylenia, & Candido, Saverio, & Skarmoutsou, Evangelia, & Amoroso, Alfredo, & Guarneri, Claudio, & Strazzanti, Angela, & Gangemi, Pietro, & Mazzarino, Maria C., & D'Amico, Fabio, & McCubrey, James A., & Libra, Massimo, & Malaponte, Grazia. (August 2012). Nectin like-5 overexpression correlates with the malignant phenotype in cutaneous melanoma. Oncotarget, 3(8), 882- 892. Retrieved from http://hdl.handle.net/10342/5699
Bevelacqua, Valentina, and Bevelacqua, Ylenia, and Candido, Saverio, and Skarmoutsou, Evangelia, and Amoroso, Alfredo, and Guarneri, Claudio, and Strazzanti, Angela, and Gangemi, Pietro, and Mazzarino, Maria C., and D'Amico, Fabio, and McCubrey, James A., and Libra, Massimo, and Malaponte, Grazia. "Nectin like-5 overexpression correlates with the malignant phenotype in cutaneous melanoma". Oncotarget. 3:8. (882-892), August 2012. December 19, 2018. http://hdl.handle.net/10342/5699.
Bevelacqua, Valentina and Bevelacqua, Ylenia and Candido, Saverio and Skarmoutsou, Evangelia and Amoroso, Alfredo and Guarneri, Claudio and Strazzanti, Angela and Gangemi, Pietro and Mazzarino, Maria C. and D'Amico, Fabio and McCubrey, James A. and Libra, Massimo and Malaponte, Grazia, "Nectin like-5 overexpression correlates with the malignant phenotype in cutaneous melanoma," Oncotarget 3, no. 8 (August 2012), http://hdl.handle.net/10342/5699 (accessed December 19, 2018).
Bevelacqua, Valentina, Bevelacqua, Ylenia, Candido, Saverio, Skarmoutsou, Evangelia, Amoroso, Alfredo, Guarneri, Claudio, Strazzanti, Angela, Gangemi, Pietro, Mazzarino, Maria C., D'Amico, Fabio, McCubrey, James A., Libra, Massimo, Malaponte, Grazia. Nectin like-5 overexpression correlates with the malignant phenotype in cutaneous melanoma. Oncotarget. August 2012; 3(8): 882-892. http://hdl.handle.net/10342/5699. Accessed December 19, 2018.