Browsing Biology by Author "Biology: Molecular Biology and Biotechnology"
-
Analysis of Complex DNA Damage Associated with Tumor Growth
Ferguson, Nicholas Foster (East Carolina University, 2012)The innate response of a fully functioning immune system's ability to recognize and react to the growth of a tumor by releasing reactive oxygen species (ROS) may lead to chronic inflammation in many cases. This chronic ... -
CHARACTERIZATION OF MIGRATING NEURAL CREST CELLS FROM HINDBRAIN EXPLANTS IN ZEBRAFISH
Murolo, Daniel (East Carolina University, 2010)Our lab was interested in creating a reproducible mechanism for obtaining neural crest cells in culture from cultured hindbrain explants of zebrafish. We have characterized the population of cells migrating from the ... -
Characterization of Site-Directed Mutants in the Cytochrome c-550 Protein of Photosystem II
Manne, Akarsh (East Carolina University, 2010)Photosynthesis is the process by which cyanobacteria, algae, and higher plants convert light energy to chemical energy via the biosynthesis of carbohydrates. Photosystem II is a multi-protein/pigment complex embedded in ... -
Characterization of the GINS subunit psf1 in Drosophila Melanogaster
Sufrinko, Brian (East Carolina University, 2010)GINS (Go Ichi Ni San; 5,1,2,3 in Japanese) is a heterotetrameric protein complex known to be essential for the establishment of DNA replication forks and the progression of the replisome. The GINS complex is known to be ... -
Consequences of Reduced Versican Expression in Embryonic Chick Synovial Joint Development
Nagchowdhuri, Partha S. (East Carolina University, 2012)The different mechanisms involved in vertebrate synovial joint development are actively being uncovered. A variety of studies have thus far discovered the involvement of several large molecules in the processes of joint ... -
DERIVATION OF TOLEROGENIC THERAPEUTIC VACCINES FOR MOUSE MODELS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
Islam, S.M. Touhidul (East Carolina University, 2012)Multiple sclerosis (MS) is a chronic inflammatory disease that results in autoimmune demyelination of the human central nervous system (CNS). Experimental autoimmune encephalomyelitis (EAE) is a widely-studied animal ... -
DISINTEGRIN-LIKE DOMAIN IN KSHV ENCODED gB IS A NECESSITY TO PROMOTE VIRUS LATENCY
Walker, Lia R. (East Carolina University, 2011)KSHV glycoprotein B (gB) is a lytic structural protein expressed on the envelope of mature virions and on the membrane of cells supporting lytic infection. Previous studies have identified that in addition to the role of ... -
Drosophila Ctf4 is essential for genome stability and normal cell cycle progression
Gosnell, Justin A. (East Carolina University, 2010)Proper DNA replication and well-timed cell cycle progression are vital to the normal functioning of a cell. Precise coordination between these mechanisms' constituent proteins ensures their processivity while safeguarding ... -
Drosophila Psf2 Has a Role In Chromosome Condensation
Chmielewski, Jeffrey Patrick (East Carolina University, 2012)The condensation state of chromosomes is a critical parameter in multiple processes within the cell. Failures in the maintenance of appropriate condensation states may lead to genomic instability, mis-expression of genes, ... -
Effects of 5-fluorouracil Drug Treatment on the Expression Profile of MicroRNAs in MCF7 Breast Cancer Cells
Shah, Maitri Yogen (East Carolina University, 2010)Breast cancer is one of the leading causes of deaths in women worldwide. 5-flourouracil (5-FU) is a classic chemotherapeutic drug that has been widely used in the treatment of breast cancer patients. In this study, using ... -
ESTABLISHING LINKAGE BETWEEN GINS COMPLEX SUB-UNIT Sld5 AND CHECKPOINT PROTEIN Chk2 (loki) USING DROSOPHILA MELANOGASTER AS MODEL ORGANISM
Devadasan, Divya (East Carolina University, 2012)Eukaryotic DNA replication is controlled by a number of proteins that ensures the process takes place accurately. GINS, a hetero-tetrameric protein complex is known to be essential for the initiation and progression of ... -
Hox Gene Expression During Oreochromis niloticus Pharyngeal Arch Development : Discovering the Hox Code
Lyon, Raymond Stewart (East Carolina University, 2010)Hox genes encode transcription factors and have been extensively studied by evolutionary and developmental biologists. Hox genes are responsible for determining specific regional identities along the anterior-posterior and ... -
Identification, Characterization, and Abiotic Stress Analysis of microRNAs in Nicotiana tabacum
Frazier, Taylor Price (East Carolina University, 2010)microRNAs (miRNAs) are non-coding RNAs with short sequences that negatively regulate gene expression at the post-transcriptional levels by either binding to mRNAs for degradation, or by inhibiting protein translation. ... -
Microbial Carbon Assimilation within the Walls of Deep-sea Hydrothermal Vent Chimneys
Blumenfeld, Heather N. (East Carolina University, 2011)Carbon is one of the most abundant elements on Earth stored in a multitude of reservoirs and constantly cycled through various processes occurring on our planet. Although it represents only a small percentage of the total ... -
Molecular Epidemiology of MRSA Among Patients and Employees in a Surgical Intensive Care Unit
Augustino, Kerri L. (East Carolina University, 2011)Methicillin-resistant Staphylococcus aureus (MRSA) is a pervasive drug resistant human pathogen and has become widespread in hospitals around the world. MRSA infections cause approximately 19,000 deaths among hospitalized ... -
Tumor Growth Induces Oxidative Clustered DNA Lesions Damage in Distant Mouse Tissue in vivo
Kryston, Thomas (East Carolina University, 2010)Our goal was to ascertain if DNA damage induced by oxidative stress is capable of being exploited as a precancerous or cancer biomarker. The specific DNA damage that we assessed was oxidatively induced non-DSB clustered ...