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Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity
(East Carolina University, 1992-08-15)
Cleavage of caldesmon with chymotrypsin yields a series of fragments which bind both calmodulin and actin and inhibit the binding of myosin subfragments to actin and the subsequent stimulation of ATPase activity. Several ...
Calponin interaction with alpha-actinin-actin: evidence for a structural role for calponin.
(East Carolina University, 1999-12)
The purpose of this study was to address the paradox of calponin localization with a-actinin and filamin, two proteins with tandem calponin homology (CH) domains, by determining the effect of these proteins on the binding ...
A Long Helix from the Central Region of Smooth Muscle Caldesmon
(East Carolina University, 1991-07-25)
The central region of smooth muscle caldesmon is predicted to form α-helices on the basis of its primary structure. We have isolated a fragment (CT54) that contains this region. The hydrodynamic
properties and the electron ...
Characterization of a caldesmon fragment that competes with myosin-ATP binding to actin.
(East Carolina University, 1993-08)
The protein caldesmon inhibits actin-activated ATP hydrolysis of myosin and inhibits the binding of myosin*ATP to actin. Afragment isolated from a chymotryptic digest of caldesmon contains features of the intact molecule ...
Equilibration and Exchange of Fluorescently Labeled Molecules in Skeletal Muscle Fibers Studied Using Confocal Microscopy
(East Carolina University, 1995-04)
Diffusion of molecules into skinned muscle fibers is often necessary when studying muscle contraction and its regulation. Usually, it is assumed that diffusion of molecules is fast also in the structured system of a muscle ...
The Interaction of Caldesmon with the COOH Terminus of Actin
(East Carolina University, 1991-10-25)
Caldesmon interacts with the NH2-terminal region of actin. It is now shown in airfuge centrifugation experiments that modification of the penultimate cysteine residue of actin significantly weakens its binding to caldesmon ...
Deoxyguanosine-resistant Leukemia L1210 Cells: Loss of Specific Deoxyribonecleoside Kinase Activity
(East Carolina University, 1993-01-05)
A mouse leukemia L1210 cell line was selected for resistance to deoxyguanosine. The deoxyguanosine-resistant cells (dGuo-R) were 126-fold less sensitive to deoxyguanosine than the wild-type cells. The IC50 values for araC ...
Kinetics of Binding of Caldesmon to Actin
(East Carolina University, 1995-04-28)
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...