The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
Author
Hermann, Brian P.; Cheng, Keren; Singh, Anukriti; Roa-De La Cruz, Lorena; Mutoji, Kazadi N.; Chen, I-Chung; Gildersleeve, Heidi; Lehle, Jake D.; Mayo, Max; Westernstroer, Birgit; Law, Nathan C.; Oatley, Melissa J.; Velte, Ellen K.; Niedenberger, Bryan A.; Fritze, Danielle; Silber, Sherman; Geyer, Christopher B.; Oatley, Jon M.; McCarrey, John R.
Abstract
Spermatogenesis is a complex and dynamic cellular differentiation process critical to male reproduc- tion and sustained by spermatogonial stem cells (SSCs). Although patterns of gene expression have been described for aggregates of certain spermato- genic cell types, the full continuum of gene expres- sion patterns underlying ongoing spermatogenesis in steady state was previously unclear. Here, we cat- alog single-cell transcriptomes for >62,000 individual spermatogenic cells from immature (postnatal day 6) and adult male mice and adult men. This allowed us to resolve SSC and progenitor spermatogonia, eluci- date the full range of gene expression changes dur- ing male meiosis and spermiogenesis, and derive unique gene expression signatures for multiple mouse and human spermatogenic cell types and/or subtypes. These transcriptome datasets provide an information-rich resource for studies of SSCs, male meiosis, testicular cancer, male infertility, or contra- ceptive development, as well as a gene expression roadmap to be emulated in efforts to achieve sper- matogenesis in vitro.
Date
2018-11-06
Citation:
APA:
Hermann, Brian P., & Cheng, Keren, & Singh, Anukriti, & Roa-De La Cruz, Lorena, & Mutoji, Kazadi N., & Chen, I-Chung, & Gildersleeve, Heidi, & Lehle, Jake D., & Mayo, Max, & Westernstroer, Birgit, & Law, Nathan C., & Oatley, Melissa J., & Velte, Ellen K., & Niedenberger, Bryan A., & Fritze, Danielle, & Silber, Sherman, & Geyer, Christopher B., & Oatley, Jon M., & McCarrey, John R.. (November 2018).
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/7995
MLA:
Hermann, Brian P., and Cheng, Keren, and Singh, Anukriti, and Roa-De La Cruz, Lorena, and Mutoji, Kazadi N., and Chen, I-Chung, and Gildersleeve, Heidi, and Lehle, Jake D., and Mayo, Max, and Westernstroer, Birgit, and Law, Nathan C., and Oatley, Melissa J., and Velte, Ellen K., and Niedenberger, Bryan A., and Fritze, Danielle, and Silber, Sherman, and Geyer, Christopher B., and Oatley, Jon M., and McCarrey, John R..
"The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids". .
. (),
November 2018.
May 07, 2024.
http://hdl.handle.net/10342/7995.
Chicago:
Hermann, Brian P. and Cheng, Keren and Singh, Anukriti and Roa-De La Cruz, Lorena and Mutoji, Kazadi N. and Chen, I-Chung and Gildersleeve, Heidi and Lehle, Jake D. and Mayo, Max and Westernstroer, Birgit and Law, Nathan C. and Oatley, Melissa J. and Velte, Ellen K. and Niedenberger, Bryan A. and Fritze, Danielle and Silber, Sherman and Geyer, Christopher B. and Oatley, Jon M. and McCarrey, John R.,
"The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids," , no.
(November 2018),
http://hdl.handle.net/10342/7995 (accessed
May 07, 2024).
AMA:
Hermann, Brian P., Cheng, Keren, Singh, Anukriti, Roa-De La Cruz, Lorena, Mutoji, Kazadi N., Chen, I-Chung, Gildersleeve, Heidi, Lehle, Jake D., Mayo, Max, Westernstroer, Birgit, Law, Nathan C., Oatley, Melissa J., Velte, Ellen K., Niedenberger, Bryan A., Fritze, Danielle, Silber, Sherman, Geyer, Christopher B., Oatley, Jon M., McCarrey, John R..
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. .
November 2018;
():
.
http://hdl.handle.net/10342/7995. Accessed
May 07, 2024.
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