Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.
Author
Qin, Zhuan; Tu, Jiagang; Lin, Tao; Norris, Steven J.; Li, Chunhao; Motaleb, Md A.
Abstract
Periplasmic flagella are essential for the distinct morphology and motility of spirochetes. A
flagella-specific type III secretion system (fT3SS) composed of a membrane-bound export
apparatus and a cytosolic ATPase complex is responsible for the assembly of the periplasmic flagella. Here, we deployed cryo-electron tomography (cryo-ET) to visualize the fT3SS
machine in the Lyme disease spirochete Borrelia burgdorferi. We show, for the first time,
that the cytosolic ATPase complex is attached to the flagellar C-ring through multiple spokes
to form the “spoke and hub” structure in B. burgdorferi. This structure not only strengthens
structural rigidity of the round-shaped C-ring but also appears to rotate with the C-ring. Our
studies provide structural insights into the unique mechanisms underlying assembly and
rotation of the periplasmic flagella and may provide the basis for the development of novel
therapeutic strategies against several pathogenic spirochetes.
Date
2018-11-09
Citation:
APA:
Qin, Zhuan, & Tu, Jiagang, & Lin, Tao, & Norris, Steven J., & Li, Chunhao, & Motaleb, Md A.. (November 2018).
Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex..
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/8433
MLA:
Qin, Zhuan, and Tu, Jiagang, and Lin, Tao, and Norris, Steven J., and Li, Chunhao, and Motaleb, Md A..
"Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.". .
. (),
November 2018.
May 20, 2024.
http://hdl.handle.net/10342/8433.
Chicago:
Qin, Zhuan and Tu, Jiagang and Lin, Tao and Norris, Steven J. and Li, Chunhao and Motaleb, Md A.,
"Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.," , no.
(November 2018),
http://hdl.handle.net/10342/8433 (accessed
May 20, 2024).
AMA:
Qin, Zhuan, Tu, Jiagang, Lin, Tao, Norris, Steven J., Li, Chunhao, Motaleb, Md A..
Cryo-electron tomography of periplasmic flagella in Borrelia burgdorferi reveals a distinct cytoplasmic ATPase complex.. .
November 2018;
():
.
http://hdl.handle.net/10342/8433. Accessed
May 20, 2024.
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