Advisor | Ables, Elizabeth Tweedie | |
Author | Beachum, Allison N | |
Date Accessioned | 2021-09-11T16:15:23Z | |
Date Available | 2023-07-01T08:02:01Z | |
Date Created | 2021-07 | |
Date of Issue | 2021-06-14 | |
xmlui.metadata.dc.date.submitted | July 2021 | |
Identifier (URI) | http://hdl.handle.net/10342/9408 | |
Description | Germ cells follow a precise and coordinated molecular timeline to produce a viable oocyte. While undergoing mitotic expansion via incomplete cytokinesis, cysts of undifferentiated cells form and remain interconnected prior to meiotic initiation, through mechanisms that are not well-defined. In somatic cells, Ras-related nuclear protein (Ran) spatiotemporally regulates mitotic spindle assembly, cleavage furrow formation and abscission. Here, we identify Ran and [beta]-importins as critical regulators of cyst development in the Drosophila ovary. Depletion of Ran or the [beta]-importins Tnpo-SR and Cadmus results in egg chambers with variable numbers of germ cells, suggesting abnormal cyst development and cyst fragmentation, and consequently disrupts oocyte selection. We demonstrate that Ran, Tnpo-SR, and Cadmus regulate key cellular processes during cyst formation, including cell cycle dynamics, fusome biogenesis, and ring canal stability, all independently of mitotic spindle assembly. Further, Tnpo-SR and Cadmus control cyclin accumulation and suppress cytokinesis, suggesting that [beta]-importins sequester protein cargos that normally promote the mitotic-to-meiotic transition. Our data demonstrates that Ran and [beta]-importins are critical for the cell fate decisions of germ cells, a role that is likely conserved in other organisms. | |
Mimetype | application/pdf | |
Language | en | |
Publisher | East Carolina University | |
Subject | stem cells | |
Subject | oocyte | |
Subject | karyopherin | |
Library of Congress Subject Headings | Drosophila--Genetic aspects | |
Library of Congress Subject Headings | Oogenesis | |
Title | The small GTPase Ran and β-importins Tnpo-SR and Cadmus promote ovarian cyst formation in Drosophila | |
Type | Master's Thesis | |
xmlui.metadata.dc.date.updated | 2021-08-30T15:41:14Z | |
Department | Biology | |
xmlui.metadata.dc.contributor.committeeMember | Anderson, Eric | |
xmlui.metadata.dc.contributor.committeeMember | Kew, Kimberly A | |
xmlui.metadata.dc.degree.name | M.S. | |
xmlui.metadata.dc.degree.level | Masters | |
xmlui.metadata.dc.degree.discipline | MS-Biology | |
xmlui.metadata.dc.degree.grantor | East Carolina University | |
xmlui.metadata.dc.degree.department | Biology | |
xmlui.metadata.dc.access.option | Restricted Campus Access Only | |
xmlui.metadata.dc.embargo.lift | 2023-07-01 | |
xmlui.metadata.dc.type.material | text | |