Browsing by Author "Gaines, Jennifer M."
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Broad-Host-Range Expression Vectors with Tightly Regulated Promoters and Their Use To Examine the Influence of TraR and TraM Expression on Ti Plasmid Quorum Sensing
Khan, Sharik R.; Gaines, Jennifer M.; Roop, R. Martin II; Farrand, Stephen K. (East Carolina University, 2008-08)Experiments requiring strong repression and precise control of cloned genes can be difficult to conduct because of the relatively high basal level of expression of currently employed promoters. We report the construction ... -
The Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice
Anderson, Eric; Paulley, James T.; Gaines, Jennifer M.; Valderas, Michelle W.; Martin, Daniel W.; Menscher, Evan A.; Brown, Timothy D.; Burns, Colin Sanderson; Roop II, R. Martin (East Carolina University, 2009-08)The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. Phenotypic analysis of an isogenic mntH mutant indicates that MntH is the sole high-affinity ...