Browsing by Author "Hughes, Robert"
-
Design, Expression, and Characterization of a Bifunctional Protein Chimera for Applications in Molecular Biology
Taylor, William (East Carolina University, 2019-05-02)There are many different methods of protein capture that are used to immobilize proteins on solid supports while still maintaining their overall function. In this study, the binding interaction between streptavidin and ... -
Investigation of Nonhydrolyzable ATP Analogues and Cofilin-Derived Peptides for Inhibition of Cofilin-Actin Rod Formation
O'Bryant, Collin Townsend (East Carolina University, 2021-08-02)Actin-Cofilin rods are an important marker of neurodegenerative disease and are commonly observed in the neurons of patients with Alzheimer's disease. The goal of this research is to investigate peptide and small-molecules ... -
OPTIMIZATION OF AN OPTOGENETICALLY ACTIVATED INITIATOR OF APOPTOSIS
Godwin, Walton Colby (East Carolina University, 2018-05-03)Optogenetic tools provide a window into the world of cell signaling pathways and protein-protein interactions with a precision and elegance that other biological probes can struggle to achieve. The creation of optogenetic ... -
SCREENING AND CHARACTERIZATION OF BIOCATALYSTS FOR SYNTHESIS OF WIELAND MIESCHER KETONE: A VERSATILE SYNTHETIC INTERMEDIATE
Patel, Mitul P (East Carolina University, 2020-12-07)Biocatalysts derived from biological molecules such as proteins, RNA, and DNA have long been exploited for applications in synthetic chemistry. Lipases, a versatile class of biocatalysts, are known to exhibit significant ... -
Square Wave Voltammetry Detection and CD Spectroscopy Structural Characterization of 5- Methyl Cytosine-Containing Oligomeric DNA Sequences
Preston, Victoria G (East Carolina University, 2023-01-04)DNA methylation, primarily at the 5-carbon in cytosine (5-methyl cytosine) plays a significant role in key processes and functions of the body, such as development of the brain and in neuronal cell differentiation. Lack ...