Browsing by Author "Li, Yong-qing"
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Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus 2 Subtilis Cells Upon Prophage Induction During Spore Germination and 3 Outgrowth, and Vegetative Growth, Using Raman Tweezers and Live-Cell 4 Imaging
Wu, Mei-yan; Li, Yong-qing; Li, Wen-wei; Christie, Graham; Setlow, Peter (2021) -
Characterization of Wet-Heat Inactivation of Single Spores of Bacillus Species by Dual-Trap Raman Spectroscopy and Elastic Light Scattering
Zhang, Pengfei; Kong, Lingbo; Setlow, Peter; Li, Yong-qing (2010-03)Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to investigate dynamic processes during high-temperature treatment of individual spores of Bacillus cereus, Bacillus megaterium, ... -
A Clostridium difficile-Specific, Gel-Forming Protein Required for Optimal Spore Germination
Donnelly, M. Lauren; Li, William; Li, Yong-qing; Hinkel, Lauren; Setlow, Peter; Shen, Aimee (2017-01-17)Clostridium difficile is a Gram-positive spore-forming obligate anaerobe that is a leading cause of antibiotic-associated diarrhea worldwide. In order for C. difficile to initiate infection, its aerotolerant spore form ... -
Kinetics of Germination of Individual Spores of Geobacillus stearothermophilus as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Zhou, Tingting; Dong, Zhiyang; Setlow, Peter; Li, Yong-qing (2013-09-13)Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very heat resistant. Raman spectroscopy and differential interference contrast microscopy were used to monitor the kinetics of ... -
Memory of Germinant Stimuli in Bacterial Spores
Wang, Shiwei; Faeder, James R.; Setlow, Peter; Li, Yong-qing (2015-11)Bacterial spores, despite being metabolically dormant, possess the remarkable capacity to detect nutrients and other molecules in their environment through a biochemical sensory apparatus that can trigger spore germination, ... -
Single-cell analysis reveals individual spore responses to simulated space vacuum
He, Lin; Wang, Shiwei; Cortesão, Marta; Wu, Muying; Moeller, Ralf; Setlow, Peter; Li, Yong-qing (2018-12-04)Outer space is a challenging environment for all forms of life, and dormant spores of bacteria have been frequently used to study the survival of terrestrial life in a space journey. Previous work showed that outer space ... -
Stable optical trapping and sensitive characterization of nanostructures using standing- wave Raman tweezers
Wu, Mu-ying; Ling, Dong-xiong; Ling, Lin; Li, William; Li, Yong-qing (2017-02-17)Optical manipulation and label-free characterization of nanoscale structures open up new possibilities for assembly and control of nanodevices and biomolecules. Optical tweezers integrated with Raman spectroscopy allows ... -
Uptake of and resistance to the antibiotic berberine by individual dormant, germinating and outgrowing bacillus spores as monitored by laser tweezers raman spectroscopy
Wang, Shiwei; Yu, Jing; Suvira, Milomir; Setlow, Peter; Li, Yong-qing (2015-12-04)Berberine, an alkaloid originally extracted from the plant Coptis chinensis and other herb plants, has been used as a pharmacological substance for many years. The therapeutic effect of berberine has been attributed to ...