Browsing by Author "Mannie, Mark"
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Development of a Human IFN-β Expression System using Chinese Hamster Ovarian Cells
Hylton, Todd Cameron (East Carolina University, 2019-05-02)Two human IFN-β expression systems were derived based on the pIRES2-AcGFP1 plasmid backbone. One expression plasmid encoded human IFN-β fused to a C-terminal linker and an 8-histidine affinity chromatography tag. A second ... -
IFN-β Facilitates Neuroantigen-Dependent Induction of CD25+ FOXP3+ Regulatory T Cells That Suppress Experimental Autoimmune Encephalomyelitis
Wang, Duncheng; Ghosh, Debjani; Islam, Touhidul; Moorman, Cody; Thomason, Ashton; Wilkinson, Daniel; Mannie, Mark (2016-10-15)This study introduces a flexible format for tolerogenic vaccination that incorporates IFN-β and neuroantigen (NAg) in the Alum adjuvant. Tolerogenic vaccination required all three components, IFN-β, NAg, and Alum, for ...