• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Other Campus Research
    • ECU Open Access Publishing Support
    • Carolina Consortium Publication Agreements
    • View Item
    •   ScholarShip Home
    • Other Campus Research
    • ECU Open Access Publishing Support
    • Carolina Consortium Publication Agreements
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes

    Thumbnail
    View/ Open
    Article (2.655Mb)

    Show full item record
    Author
    Koch, Peter J.; Webb, Saiphone; Gugger, Jessica A.; Salois, Maddison N.; Koster, Maranke I.
    Abstract
    Investigating basic biological mechanisms underlying human diseases relies on the availability of sufficient quantities of patient cells. As most primary somatic cells have a limited lifespan, obtaining sufficient material for biological studies has been a challenge. The development of induced pluripotent stem cell (iPSC) technology has been a game changer, especially in the field of rare genetic disorders. iPSC are essentially immortal, can be stored indefinitely, and can thus be used to generate defined somatic cells in unlimited quantities. Further, the availability of genome editing technologies, such as CRISPR/CAS, has provided us with the opportunity to create “designer” iPSC lines with defined genetic characteristics. A major advancement in biological research stems from the development of methods to direct iPSC differentiation into defined cell types. In this article, we provide the basic protocol for the generation of human iPSC-derived keratinocytes (iPSC-K). These cells have the characteristics of basal epidermal keratinocytes and represent a tool for the investigation of normal epidermal biology, as well as genetic and acquired skin disorders. © 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
    URI
    http://hdl.handle.net/10342/10977
    Subject
     epidermal biology; iPSC; induced pluripotent stem cell; iPSC-derived keratinocytes; keratinocytes; skin diseases 
    Date
    2022-04-05
    Citation:
    APA:
    Koch, Peter J., & Webb, Saiphone, & Gugger, Jessica A., & Salois, Maddison N., & Koster, Maranke I.. (April 2022). Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes. , (), - . Retrieved from http://hdl.handle.net/10342/10977

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Koch, Peter J., and Webb, Saiphone, and Gugger, Jessica A., and Salois, Maddison N., and Koster, Maranke I.. "Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes". . . (), April 2022. February 02, 2023. http://hdl.handle.net/10342/10977.
    Chicago:
    Koch, Peter J. and Webb, Saiphone and Gugger, Jessica A. and Salois, Maddison N. and Koster, Maranke I., "Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes," , no. (April 2022), http://hdl.handle.net/10342/10977 (accessed February 02, 2023).
    AMA:
    Koch, Peter J., Webb, Saiphone, Gugger, Jessica A., Salois, Maddison N., Koster, Maranke I.. Differentiation of Human Induced Pluripotent Stem Cells into Keratinocytes. . April 2022; (): . http://hdl.handle.net/10342/10977. Accessed February 02, 2023.
    Collections
    • Carolina Consortium Publication Agreements

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback