• Find People
  • Campus Map
  • PiratePort
  • A-Z
    • About
    • Submit
    • Browse
    • Login
    View Item 
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Pharmacology and Toxicology
    • View Item
    •   ScholarShip Home
    • Division of Health Sciences
    • Brody School of Medicine
    • Pharmacology and Toxicology
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of The ScholarShipCommunities & CollectionsDateAuthorsTitlesSubjectsTypeDate SubmittedThis CollectionDateAuthorsTitlesSubjectsTypeDate Submitted

    My Account

    Login

    Statistics

    View Google Analytics Statistics

    Perfluorooctanoic acid-induced developmental cardiotoxicity in an avian model

    Thumbnail
    View/ Open
    Jiang_ecu_0600D_10876.pdf (3.041Mb)

    Show full item record
    Author
    Jiang, Qixiao
    Abstract
    Perfluorooctanoic acid (PFOA) is a synthetic chemical used in polyfluormer production. It is a widespread environmental contaminant and is known to induce developmental toxicity in rodent models. As several related compounds also induce developmental cardiotoxicity in avian models, a series of experiments were carried out in chicken embryos and hatchling chickens to assess the effects of PFOA on developing hearts and to explore underlying mechanisms. Histology was used to assess late stage embryo hearts and echocardiography was used to assess morphological and functional parameters of hearts in living one-day-old hatchlings. Hearts of exposed embryos had a thinned right ventricular wall and multiple morphological and functional changes were detected in the left ventricles of exposed hatchlings. As PFOA is known to activate the peroxisome proliferator activated receptor á (PPARá) in rodent models, the PPARá agonist WY 14,643 was used to assess the role of PPARá in PFOA-induced developmental cardiotoxicity. Real-time polymerase chain reaction (PCR) and western blot were used to assess hearts of early stage embryos for expression of genes related to heart development. WY 14,643 partially mimicked the morphological and functional effects of PFOA only in the left ventricle of hearts of hatchling chickens and bone morphogenic protein 2 (BMP2) signaling pathways were involved in mediating the process. Additionally, primary cardiomyocyte cultures were used to explore mechanisms of toxicity at the cellular level. Viability, contractility, and reactive oxygen species (ROS) generation were evaluated in primary cardiomyocytes isolated from hearts of embryos exposed to PFOA in ovo or were exposed in vitro. Primary cardiomyocytes isolated from embryos exposed to PFOA in ovo functioned similarly to hearts of exposed hatchling chickens. Primary cardiomyocytes exposed in vitro responded differently, suggesting that the cardiotoxicity is likely from interference of PFOA in an early stage of heart development rather than from overt cytotoxicity to cardiomyocytes. In conclusion, this dissertation work indicates that PFOA induces morphological and functional alterations in hearts of chicken embryos and hatchling chickens, that mechanisms of the cardiotoxicity may involve PPARá and BMP2 signaling pathways, and that PFOA is likely interfering in an early stage of heart development.  
    URI
    http://hdl.handle.net/10342/1818
    Subject
     Toxicology; Avian; Cardiotoxicity; Developmental toxicity; Perfluorooctanoic acid 
    Date
    2013
    Citation:
    APA:
    Jiang, Qixiao. (January 2013). Perfluorooctanoic acid-induced developmental cardiotoxicity in an avian model (Doctoral Dissertation, East Carolina University). Retrieved from the Scholarship. (http://hdl.handle.net/10342/1818.)

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Jiang, Qixiao. Perfluorooctanoic acid-induced developmental cardiotoxicity in an avian model. Doctoral Dissertation. East Carolina University, January 2013. The Scholarship. http://hdl.handle.net/10342/1818. March 07, 2021.
    Chicago:
    Jiang, Qixiao, “Perfluorooctanoic acid-induced developmental cardiotoxicity in an avian model” (Doctoral Dissertation., East Carolina University, January 2013).
    AMA:
    Jiang, Qixiao. Perfluorooctanoic acid-induced developmental cardiotoxicity in an avian model [Doctoral Dissertation]. Greenville, NC: East Carolina University; January 2013.
    Collections
    • Dissertations
    • Pharmacology and Toxicology
    Publisher
    East Carolina University

    xmlui.ArtifactBrowser.ItemViewer.elsevier_entitlement

    East Carolina University has created ScholarShip, a digital archive for the scholarly output of the ECU community.

    • About
    • Contact Us
    • Send Feedback