Alteration of In Vivo Cellulose Ribbon Assembly by Carboxymethylcellulose and Other Polysaccharides
Author
White, Alan; Haigler, Candace H.; Cooper, Kay M.; Brown, R. Malcolm Jr
Abstract
In vivo cellulose ribbon assembly by the Gram-negative bacterium Acetobacter xylinum can be altered by incubation in carboxymethylcellulose (CMC), a negatively charged water-soluble cellulose derivative, and also by incubation in a variety of neutral, water-soluble cellulose derivatives. In the presence of all of these substituted celluloses, normal fasciation of microfibril bundles to form the typical twisting ribbon is prevented. Alteration of ribbon assembly is most extensive in the presence of CMC, which often induces synthesis of separate, intertwining bundles of microfibrils. Freeze- etch preparations of the bacterial outer membrane suggest that particles that are thought to be associated with cellulose synthesis or extrusion may be specifically organized to mediate synthesis of microfibril bundles. These data support the previous hypothesis that the cellulose ribbon of A. xylinum is formed by a hierarchical, cell- directed, self-assembly process. The relationship of these results to the regulation of cellulose microfibril size and wall extensibility in plant cell walls is discussed.
Date
1982
Citation:
APA:
White, Alan, & Haigler, Candace H., & Cooper, Kay M., & Brown, R. Malcolm Jr. (January 1982).
Alteration of In Vivo Cellulose Ribbon Assembly by Carboxymethylcellulose and Other Polysaccharides.
,
(),
-
. Retrieved from
http://hdl.handle.net/10342/1934
MLA:
White, Alan, and Haigler, Candace H., and Cooper, Kay M., and Brown, R. Malcolm Jr.
"Alteration of In Vivo Cellulose Ribbon Assembly by Carboxymethylcellulose and Other Polysaccharides". .
. (),
January 1982.
December 08, 2023.
http://hdl.handle.net/10342/1934.
Chicago:
White, Alan and Haigler, Candace H. and Cooper, Kay M. and Brown, R. Malcolm Jr,
"Alteration of In Vivo Cellulose Ribbon Assembly by Carboxymethylcellulose and Other Polysaccharides," , no.
(January 1982),
http://hdl.handle.net/10342/1934 (accessed
December 08, 2023).
AMA:
White, Alan, Haigler, Candace H., Cooper, Kay M., Brown, R. Malcolm Jr.
Alteration of In Vivo Cellulose Ribbon Assembly by Carboxymethylcellulose and Other Polysaccharides. .
January 1982;
():
.
http://hdl.handle.net/10342/1934. Accessed
December 08, 2023.
Collections
Publisher
East Carolina University