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    Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells

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    Author
    Chen, Yan-Hua; Lu, Qun; Goodenough, Daniel A.; Jeansonne, Beverly
    Abstract
    Occludin is an integral membrane protein that is tyrosine phosphorylated when localized at tight junctions. When Ca2 was depleted from the culture medium, occludin tyrosine phosphorylation was diminished from Madin-Darby canine kidney epithelial cells in 2 min. This dephosphorylation was correlated with a significant reduction in transepithelial electrical resistance (TER), indicating a global loss of the tight junction barrier function. Reconstitution of Ca2 resulted in a robust tyrosine rephosphorylation of occludin that was temporally associated with an increase in TER. Moreover, we demonstrate in this study that occludin was colocalized with the nonreceptor tyrosine kinase c-Yes at cell junction areas and formed an immunoprecipitable complex with c-Yes in vivo. This complex dissociated when the cells were incubated in medium without Ca2 or treated with a c-Yes inhibitor, CGP77675. In the presence of CGP77675 after Ca2 repletion, occludin tyrosine phosphorylation was completely abolished and both tight junction formation and the increase of the TER were inhibited. Our study thus provides strong evidence that occludin tyrosine phosphorylation is tightly linked to tight junction formation in epithelial cells, and that the nonreceptor tyrosine kinase c-Yes is involved in the regulation of this process. Originally published in Molecular Biology of the Cell, April 2002 13:4.
    URI
    http://hdl.handle.net/10342/2975
    Subject
     Occludin; Membrane Proteins; Tyrosine kinase; Protein phosphorylation 
    Date
    2002-04
    Citation:
    APA:
    Chen, Yan-Hua, & Lu, Qun, & Goodenough, Daniel A., & Jeansonne, Beverly. (April 2002). Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells. Molecular Biology of the Cell, 13(4), 1227- 1237. Retrieved from http://hdl.handle.net/10342/2975

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Chen, Yan-Hua, and Lu, Qun, and Goodenough, Daniel A., and Jeansonne, Beverly. "Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells". Molecular Biology of the Cell. 13:4. (1227-1237), April 2002. September 30, 2023. http://hdl.handle.net/10342/2975.
    Chicago:
    Chen, Yan-Hua and Lu, Qun and Goodenough, Daniel A. and Jeansonne, Beverly, "Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells," Molecular Biology of the Cell 13, no. 4 (April 2002), http://hdl.handle.net/10342/2975 (accessed September 30, 2023).
    AMA:
    Chen, Yan-Hua, Lu, Qun, Goodenough, Daniel A., Jeansonne, Beverly. Nonreceptor Tyrosine Kinase c-Yes Interacts with Occludin during Tight Junction Formation in Canine Kidney Epithelial Cells. Molecular Biology of the Cell. April 2002; 13(4): 1227-1237. http://hdl.handle.net/10342/2975. Accessed September 30, 2023.
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    • Pharmacology and Toxicology
    Publisher
    East Carolina University

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