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    Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity

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    Author
    Chalovich, Joseph M.; Bryan, Joseph; Benson, Caryl E.; Velaz, Laly
    Abstract
    Cleavage of caldesmon with chymotrypsin yields a series of fragments which bind both calmodulin and actin and inhibit the binding of myosin subfragments to actin and the subsequent stimulation of ATPase activity. Several of these fragments have been purified by cation exchange chromatography and their amino-terminal sequences determined. The smallest fragment has a molecular mass of about 7.3 kDa and extends from Leu597 to Phe665. This polypeptide inhibits the actin-activated ATPase of myosin S-1; this inhibition is augmented by smooth muscle tropomyosin and relieved by Ca2+- calmodulin. The binding of the 7.3-kDa fragment to actin is competitive with the binding of S - 1 to actin. Thus, this polypeptide has several of the important features characteristic of intact caldesmon. However, although an intact caldesmon molecule covers between six and nine actin monomers, the 7.3-kDa fragment binds to actin in a 1:1 complex. Comparison of this fragment with others suggests that a small region of caldesmon is responsible for at least part of the interaction with both calmodulin and actin. Originally published in the Journal of Biological Chemistry, vol. 267, No. 23, 1992
    URI
    http://hdl.handle.net/10342/2993
    Subject
     Caldesmon; Calmodulin; Actin 
    Date
    1992-08-15
    Citation:
    APA:
    Chalovich, Joseph M., & Bryan, Joseph, & Benson, Caryl E., & Velaz, Laly. (August 1992). Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity. , (. Retrieved from http://hdl.handle.net/10342/2993

    Display/Hide MLA, Chicago and APA citation formats.

    MLA:
    Chalovich, Joseph M., and Bryan, Joseph, and Benson, Caryl E., and Velaz, Laly. "Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity". . . (.), August 1992. August 09, 2022. http://hdl.handle.net/10342/2993.
    Chicago:
    Chalovich, Joseph M. and Bryan, Joseph and Benson, Caryl E. and Velaz, Laly, "Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity,"  , no. (August 1992), http://hdl.handle.net/10342/2993 (accessed August 09, 2022).
    AMA:
    Chalovich, Joseph M., Bryan, Joseph, Benson, Caryl E., Velaz, Laly. Localization and Characterization of a 7.3-kDa Region of Caldesmon Which Reversibly Inhibits Actomyosin ATPase Activity. . August 1992; () . http://hdl.handle.net/10342/2993. Accessed August 09, 2022.
    Collections
    • Biochemistry and Molecular Biology
    Publisher
    East Carolina University

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