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    Predifferentiated Embryonic Stem Cells Prevent Chronic Pain Behaviors and Restore Sensory Function Following Spinal Cord Injury in Mice

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    Author
    Hendricks, Wesley A.; Pak, Elena S.; Owensby, Paul J.; Menta, Kristie J.; Glazova, Margarita; Moretto, Justin; Hollis, Sarah Elizabeth; Brewer, Kori L.; Murashov, Alexander K.
    Abstract
    Embryonic stem (ES) cells have been investigated in repair of the CNS following neuronal injury and disease; however, the efficacy of these cells in treatment of postinjury pain is far from clear. In this study, we evaluated the therapeutic potential of predifferentiated mouse ES cells to restore sensory deficits following spinal cord injury (SCI) in mice. The pain model used unilateral intraspinal injection of quisqualic acid (QUIS) into the dorsal horn between vertebral levels T13 and L1. Seven days later, 60,000 predifferentiated ES cells or media were transplanted into the site of the lesion. Histological analysis at 7, 14, and 60 days posttransplantation revealed that animals receiving ES cell transplants suffered significantly less tissue damage than animals receiving media alone. Transplanted cells provided immediate effects on both spontaneous and evoked pain behaviors. Treatment with ES cells resulted in 0% (n = 28) excessive grooming behavior versus 60% (18 of 30) in media-treated animals. In the acetone test (to assess thermal allodynia), mice recovered to preinjury levels by 12 days after ES cell transplant, whereas control animals injected with media after SCI did not show any improvement up to 60 days. Similarly, the von Frey test (to assess mechanical allodynia) and the formalin test (to assess nociceptive hyperalgesia) showed that transplantation of predifferentiated ES cells significantly reduced these pain behaviors following injury. Here we show that predifferentiated ES cells act in a neuroprotective manner and provide antinociceptive and therapeutic effects following excitotoxic SCI.Originally published in Molecular Medicine Vol. 12, No. 1-3 2006.
    URI
    http://hdl.handle.net/10342/3012
    Subject
     Embryonic stem cells; Neuronal injuries; Spinal cord injuries 
    Date
    2006
    Citation:
    APA:
    Hendricks, Wesley A., & Pak, Elena S., & Owensby, Paul J., & Menta, Kristie J., & Glazova, Margarita, & Moretto, Justin, & Hollis, Sarah Elizabeth, & Brewer, Kori L., & Murashov, Alexander K.. (January 2006). Predifferentiated Embryonic Stem Cells Prevent Chronic Pain Behaviors and Restore Sensory Function Following Spinal Cord Injury in Mice. , (. Retrieved from http://hdl.handle.net/10342/3012

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    MLA:
    Hendricks, Wesley A., and Pak, Elena S., and Owensby, Paul J., and Menta, Kristie J., and Glazova, Margarita, and Moretto, Justin, and Hollis, Sarah Elizabeth, and Brewer, Kori L., and Murashov, Alexander K.. "Predifferentiated Embryonic Stem Cells Prevent Chronic Pain Behaviors and Restore Sensory Function Following Spinal Cord Injury in Mice". . . (.), January 2006. August 11, 2022. http://hdl.handle.net/10342/3012.
    Chicago:
    Hendricks, Wesley A. and Pak, Elena S. and Owensby, Paul J. and Menta, Kristie J. and Glazova, Margarita and Moretto, Justin and Hollis, Sarah Elizabeth and Brewer, Kori L. and Murashov, Alexander K., "Predifferentiated Embryonic Stem Cells Prevent Chronic Pain Behaviors and Restore Sensory Function Following Spinal Cord Injury in Mice,"  , no. (January 2006), http://hdl.handle.net/10342/3012 (accessed August 11, 2022).
    AMA:
    Hendricks, Wesley A., Pak, Elena S., Owensby, Paul J., Menta, Kristie J., Glazova, Margarita, Moretto, Justin, Hollis, Sarah Elizabeth, Brewer, Kori L., Murashov, Alexander K.. Predifferentiated Embryonic Stem Cells Prevent Chronic Pain Behaviors and Restore Sensory Function Following Spinal Cord Injury in Mice. . January 2006; () . http://hdl.handle.net/10342/3012. Accessed August 11, 2022.
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    • Physiology
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    East Carolina University

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