Physical and Functional Interactions between the Herpes Simplex Virus UL15 and UL28 DNA Cleavage and Packaging Proteins

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Author
Koslowski, Kim M.; Shaver, Patti R.; Casey, James T. II; Wilson, Todd; Yamanaka, Gregory; Sheaffer, Amy K.; Tenney, Daniel J.; Pederson, Nels E.
Abstract
Herpes simplex virus (HSV) DNA is cleaved from concatemers and packaged into capsids in infected cell nuclei. This process requires seven viral proteins, including UL15 and UL28. UL15 expressed alone displays a nuclear localization, while UL28 remains cytoplasmic. Coexpression with UL15 enables UL28 to enter nuclei, suggesting an interaction between the two proteins. Additionally, UL28 copurified with UL15 from HSV-infected cells after ion-exchange and DNA affinity chromatography, and the complex sedimented as a 1:1 heterodimer upon sucrose gradient centrifugation. These findings are evidence of a physical interaction of UL15 and UL28 and a functional role for UL15 in directing UL28 to the nucleus. Originally published in Journal of Virology, 1999 Vol. 73, No. 2.
URI
http://hdl.handle.net/10342/3034
Subject
 Herpes simplex virus; Viral Proteins 
Date
1999-02
Citation:
APA:
Koslowski, Kim M., & Shaver, Patti R., & Casey, James T. II, & Wilson, Todd, & Yamanaka, Gregory, & Sheaffer, Amy K., & Tenney, Daniel J., & Pederson, Nels E.. (February 1999). Physical and Functional Interactions between the Herpes Simplex Virus UL15 and UL28 DNA Cleavage and Packaging Proteins. Journal of Virology, 73(2), 1704- 1707. Retrieved from http://hdl.handle.net/10342/3034

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MLA:
Koslowski, Kim M., and Shaver, Patti R., and Casey, James T. II, and Wilson, Todd, and Yamanaka, Gregory, and Sheaffer, Amy K., and Tenney, Daniel J., and Pederson, Nels E.. "Physical and Functional Interactions between the Herpes Simplex Virus UL15 and UL28 DNA Cleavage and Packaging Proteins". Journal of Virology. 73:2. (1704-1707), February 1999. December 01, 2023. http://hdl.handle.net/10342/3034.
Chicago:
Koslowski, Kim M. and Shaver, Patti R. and Casey, James T. II and Wilson, Todd and Yamanaka, Gregory and Sheaffer, Amy K. and Tenney, Daniel J. and Pederson, Nels E., "Physical and Functional Interactions between the Herpes Simplex Virus UL15 and UL28 DNA Cleavage and Packaging Proteins," Journal of Virology 73, no. 2 (February 1999), http://hdl.handle.net/10342/3034 (accessed December 01, 2023).
AMA:
Koslowski, Kim M., Shaver, Patti R., Casey, James T. II, Wilson, Todd, Yamanaka, Gregory, Sheaffer, Amy K., Tenney, Daniel J., Pederson, Nels E.. Physical and Functional Interactions between the Herpes Simplex Virus UL15 and UL28 DNA Cleavage and Packaging Proteins. Journal of Virology. February 1999; 73(2): 1704-1707. http://hdl.handle.net/10342/3034. Accessed December 01, 2023.
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East Carolina University