Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces
Author
Tsuji, Shoutaro; Yamashita, Makiko; Hoffman, Donald R.; Nishiyama, Akihito; Shinohara, Tsutomu; Ohtsu, Takashi; Shibata, Yoshimi
Abstract
Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum protein and bound to Mycobacterium bovis bacillus Calmette-Gu´erin (BCG). Human intelectin-1-binding to BCG was inhibited by Ca2+- depletion, galactofuranosyl disaccharide, ribose, or xylose, and was dependent on the trimeric structure of human intelectin-1. Although monomeric, mouse intelectin-1 bound to BCG, with its C-terminal region contributing to efficient binding. Human intelectin-1-transfected cells not
only secreted intelectin-1 into culture supernatant but also expressed intelectin-1 on the cell surface. The cell surface intelectin-1 was not a glycosylphosphatidylinositolanchored membrane protein. Intelectin-1-transfected cells captured BCG more than untransfected cells, and the BCG adherence was inhibited by an inhibitory saccharide of intelectin-1. Intelectin-1-preincubated cells took up BCG more than untreated cells, but the adhesion of intelectin-1- bound BCG was the same as that of untreated BCG. Mouse macrophages phagocytosedBCGmore efficiently in medium containing mouse intelectin-1 than in control medium. These results indicate that intelectin is a host defense lectin that assists phagocytic clearance of microorganisms. Originally published Glycobiology, Vol. 19, No. 5, May 2009
Subject
Date
2009-05
Citation:
APA:
Tsuji, Shoutaro, & Yamashita, Makiko, & Hoffman, Donald R., & Nishiyama, Akihito, & Shinohara, Tsutomu, & Ohtsu, Takashi, & Shibata, Yoshimi. (May 2009).
Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces.
Glycobiology,
19(5),
518-
526. Retrieved from
http://hdl.handle.net/10342/3041
MLA:
Tsuji, Shoutaro, and Yamashita, Makiko, and Hoffman, Donald R., and Nishiyama, Akihito, and Shinohara, Tsutomu, and Ohtsu, Takashi, and Shibata, Yoshimi.
"Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces". Glycobiology.
19:5. (518-526),
May 2009.
September 26, 2023.
http://hdl.handle.net/10342/3041.
Chicago:
Tsuji, Shoutaro and Yamashita, Makiko and Hoffman, Donald R. and Nishiyama, Akihito and Shinohara, Tsutomu and Ohtsu, Takashi and Shibata, Yoshimi,
"Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces," Glycobiology 19, no.
5 (May 2009),
http://hdl.handle.net/10342/3041 (accessed
September 26, 2023).
AMA:
Tsuji, Shoutaro, Yamashita, Makiko, Hoffman, Donald R., Nishiyama, Akihito, Shinohara, Tsutomu, Ohtsu, Takashi, Shibata, Yoshimi.
Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces. Glycobiology.
May 2009;
19(5):
518-526.
http://hdl.handle.net/10342/3041. Accessed
September 26, 2023.
Collections
Publisher
East Carolina University