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    Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces

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    Author
    Tsuji, Shoutaro; Yamashita, Makiko; Hoffman, Donald R.; Nishiyama, Akihito; Shinohara, Tsutomu; Ohtsu, Takashi; Shibata, Yoshimi
    Abstract
    Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum protein and bound to Mycobacterium bovis bacillus Calmette-Gu´erin (BCG). Human intelectin-1-binding to BCG was inhibited by Ca2+- depletion, galactofuranosyl disaccharide, ribose, or xylose, and was dependent on the trimeric structure of human intelectin-1. Although monomeric, mouse intelectin-1 bound to BCG, with its C-terminal region contributing to efficient binding. Human intelectin-1-transfected cells not only secreted intelectin-1 into culture supernatant but also expressed intelectin-1 on the cell surface. The cell surface intelectin-1 was not a glycosylphosphatidylinositolanchored membrane protein. Intelectin-1-transfected cells captured BCG more than untransfected cells, and the BCG adherence was inhibited by an inhibitory saccharide of intelectin-1. Intelectin-1-preincubated cells took up BCG more than untreated cells, but the adhesion of intelectin-1- bound BCG was the same as that of untreated BCG. Mouse macrophages phagocytosedBCGmore efficiently in medium containing mouse intelectin-1 than in control medium. These results indicate that intelectin is a host defense lectin that assists phagocytic clearance of microorganisms. Originally published Glycobiology, Vol. 19, No. 5, May 2009
    URI
    http://hdl.handle.net/10342/3041
    Subject
     Galactofuranose; Innate immunity; Intelectin; Lectin; Mycobacteria 
    Date
    2009-05
    Citation:
    APA:
    Tsuji, Shoutaro, & Yamashita, Makiko, & Hoffman, Donald R., & Nishiyama, Akihito, & Shinohara, Tsutomu, & Ohtsu, Takashi, & Shibata, Yoshimi. (May 2009). Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces. Glycobiology, (19:5), p.518-526. Retrieved from http://hdl.handle.net/10342/3041

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    MLA:
    Tsuji, Shoutaro, and Yamashita, Makiko, and Hoffman, Donald R., and Nishiyama, Akihito, and Shinohara, Tsutomu, and Ohtsu, Takashi, and Shibata, Yoshimi. "Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces". Glycobiology. 19:5. (518-526.), May 2009. April 19, 2021. http://hdl.handle.net/10342/3041.
    Chicago:
    Tsuji, Shoutaro and Yamashita, Makiko and Hoffman, Donald R. and Nishiyama, Akihito and Shinohara, Tsutomu and Ohtsu, Takashi and Shibata, Yoshimi, "Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces," Glycobiology 19, no. 5 (May 2009), http://hdl.handle.net/10342/3041 (accessed April 19, 2021).
    AMA:
    Tsuji, Shoutaro, Yamashita, Makiko, Hoffman, Donald R., Nishiyama, Akihito, Shinohara, Tsutomu, Ohtsu, Takashi, Shibata, Yoshimi. Capture of heat-killed Mycobacterium bovis bacillus Calmette-Guérin by intelectin-1deposited on cell surfaces. Glycobiology. May 2009; 19(5) 518-526. http://hdl.handle.net/10342/3041. Accessed April 19, 2021.
    Collections
    • Pathology and Laboratory Medicine
    Publisher
    East Carolina University

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