Identification and mapping of two divergent, unlinked major histocompatibility complex class II B genes in Xiphophorus fishes.
Author
McConnell, Thomas J.; Godwin, Ulla B.; Norton, Stephen F.; Nairn, Rodney S.; Kazianis, Steven; Morizot, Donald C.
Abstract
We have isolated two major histocompatibility complex (MHC) class II B genes from the inbred fish
strain Xiphophorus maculatus Jp 163 A. We mapped one of these genes, designated here as DXB, to linkage
group III, linked to a malic enzyme locus, also syntenic with human and mouse MHC. Comparison of
genomic and cDNA clones shows the gene consists of six exons and five introns. The encoded b1 domain
has three amino acids deleted and a cytoplasmic tail nine amino acids longer than in other teleost class
II b chains, more similar to HLA-DRB, clawed frog Xela-F3, and nurse shark Gici-B. Key residues for
disulfide bonds, glycosylation, and interaction with a chains are conserved. These same features are also
present in a swordtail (Xiphophorus helleri) genomic DXB PCR clone. A second type of class II B clone was
amplified by PCR from X. maculatus and found to be orthologous to class II genes identified in other
fishes. This DAB-like gene is 63% identical to the X. maculatus DXB sequence in the conserved b2-encoding
exon and was mapped to new unassigned linkage group LG U24. The DXB gene, then, represents an
unlinked duplicated locus not previously identified in teleosts. Originally published Genetics Vol. 149, No. 4, August 1998
Date
1998-08
Citation:
APA:
McConnell, Thomas J., & Godwin, Ulla B., & Norton, Stephen F., & Nairn, Rodney S., & Kazianis, Steven, & Morizot, Donald C.. (August 1998).
Identification and mapping of two divergent, unlinked major histocompatibility complex class II B genes in Xiphophorus fishes..
Genetics,
149(4),
1921-
1934. Retrieved from
http://hdl.handle.net/10342/3050
MLA:
McConnell, Thomas J., and Godwin, Ulla B., and Norton, Stephen F., and Nairn, Rodney S., and Kazianis, Steven, and Morizot, Donald C..
"Identification and mapping of two divergent, unlinked major histocompatibility complex class II B genes in Xiphophorus fishes.". Genetics.
149:4. (1921-1934),
August 1998.
September 22, 2023.
http://hdl.handle.net/10342/3050.
Chicago:
McConnell, Thomas J. and Godwin, Ulla B. and Norton, Stephen F. and Nairn, Rodney S. and Kazianis, Steven and Morizot, Donald C.,
"Identification and mapping of two divergent, unlinked major histocompatibility complex class II B genes in Xiphophorus fishes.," Genetics 149, no.
4 (August 1998),
http://hdl.handle.net/10342/3050 (accessed
September 22, 2023).
AMA:
McConnell, Thomas J., Godwin, Ulla B., Norton, Stephen F., Nairn, Rodney S., Kazianis, Steven, Morizot, Donald C..
Identification and mapping of two divergent, unlinked major histocompatibility complex class II B genes in Xiphophorus fishes.. Genetics.
August 1998;
149(4):
1921-1934.
http://hdl.handle.net/10342/3050. Accessed
September 22, 2023.
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Publisher
East Carolina University